Download or read book Purification and Characterization of Penicillin binding Protein 2 of Escherichia Coli written by Ora K. Dillon-Carter and published by . This book was released on 1983 with total page 54 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book The Purification and Characterization of a DNA binding Protein Induced by Infection of Escherichia Coli with Bacteriophage written by Roberta Charlotte Reuben and published by . This book was released on 1973 with total page 290 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book The Oligopeptide Permease of Escherichia Coli written by Cheryl Ann Guyer and published by . This book was released on 1985 with total page 204 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book The Membrane Topology of Penicillin binding Protein 3 of Escherichia Coli written by and published by . This book was released on 1990 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this thesis I present a study of the membrane topology of PBP3 of Escherichia coli and examine its suitability as a candidate for crystallisation and subsequent X-ray diffraction studies.The bacteriocidal activity of the 3-lactam antibiotics resides in their ability to bind to and inactivate a series of enzymes that mediate the final stages in peptidoglycan synthesis. There are seven of these penicillin-binding proteins (PBP's) present in Escherichia coli. The four of highest molecular weight (PBP's 1A, IB, 2 and 3) appear essential for cell growth, and since PBP3 has the highest affinity for most of the 3-lactams in current clinical use, it is, from a medical perspective, probably the most important killing target.In the first part of this thesis I present an analysis of the membrane organisation of PBP3. A series of gene fusions were constructed between the coding region of mature ITEM 3-lactamase and random positions within the PBP3 gene. The position of the fusion junction in 84 in-frame fusions was determined, with fusions to 61 different positions in PBP3 being obtained. Fusions to positions within the first 31 residues of PBP3 resulted in enzymically active fusion proteins which were unable to protect single cells of E.coli from killing by ampicillin, indicating that the 3-lactamase moieties of these fusion proteins were not translocated to the periplasm. However, all in-frame fusions containing more than 35 residues of PBP3 were able to provide single cells of E.coli with appreciable levels of resistance to ampicillin, indicating that the B-lacamase moieties of these fusion proteins were translocated to the periplasm. This data therefore implies that PBP3 has a simple membrane topology with the bulk of the protein (residues 36 to the carboxy-terminus) exposed on the periplasmic side of the inner membrane. The pattern of proteolytic sensitivity of PBP3 in both spheroplasts and membrane vesicles later confirmed this model. That PBP3 was only anchored to the inner membrane through its NHa-terminus has been shown since replacement of its putative lipoprotein signal with a normal cleaved signal peptide resulted in a water-soluble, periplasmic form of the enzyme which still bound penicillin. The last part of this thesis concerns the characterisation of this soluble PBP, and examines the possibilities for its subsequent purification.

Download or read book The Membrane Topology of Penicillin binding Protein 3 of Escherichia Coli written by Lucas David Bowler and published by . This book was released on 1990 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book The Purification and Characterization of the Escherichia Coli Helicase II Protein and a Study of the Protein s Enzymatic Activities written by Gregory Thomas Runyon and published by . This book was released on 1991 with total page 620 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Bacterial Cell Wall written by J.-M. Ghuysen and published by Elsevier. This book was released on 1994-02-09 with total page 607 pages. Available in PDF, EPUB and Kindle. Book excerpt: Studies of the bacterial cell wall emerged as a new field of research in the early 1950s, and has flourished in a multitude of directions. This excellent book provides an integrated collection of contributions forming a fundamental reference for researchers and of general use to teachers, advanced students in the life sciences, and all scientists in bacterial cell wall research. Chapters include topics such as: Peptidoglycan, an essential constituent of bacterial endospores; Teichoic and teichuronic acids, lipoteichoic acids, lipoglycans, neural complex polysaccharides and several specialized proteins are frequently unique wall-associated components of Gram-positive bacteria; Bacterial cells evolving signal transduction pathways; Underlying mechanisms of bacterial resistance to antibiotics.

Download or read book Enzyme Technologies for Pharmaceutical and Biotechnological Applications written by Herbert A Kirst and published by CRC Press. This book was released on 2001-06-05 with total page 642 pages. Available in PDF, EPUB and Kindle. Book excerpt: A review of enzyme function in human and animal health, this book covers basic principles and applications in antibiotic biosynthesis, biocatalysis, and screening and assay optimization, as well as new and emerging technologies in the biotechnological and pharmaceutical industries. It offers real-world examples of timely laboratory, pharmaceutical and manufacturing processes, including large-scale applications of enzyme manipulations.

Download or read book Cumulated Index Medicus written by and published by . This book was released on 1999 with total page 1860 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book The Amphipathic Helix written by Richard M. Epand and published by CRC Press. This book was released on 1993-07-09 with total page 422 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Amphipathic Helix is a comprehensive volume discussing amphipathic helices in systems as diverse as serum lipoproteins, lung surfactant, cytotoxic peptides, ion channels, mitochondrial targeting, peptide hormones, G proteins, T-cell recognition, DNA binding proteins, and antifreeze proteins. The book also includes general introductory material that defines amphipathic helices, discusses methods to identify amphipathic helical segments from the amino acid sequence of a protein, illustrates how amphipathic helices can be used in the de novo design of peptide and protein structures, and describes how these helices stabilize protein structures. There is also a section on techniques to determine helix orientation in a membrane environment using polarized attenuated total reflection infrared spectroscopy or solid state NMR spectroscopy. Recent developments on all these topics have been discussed by leading experts in this reference for researchers and students in biochemistry, biophysics, and pharmacology.

Download or read book Macromolecular Protein Complexes II Structure and Function written by J. Robin Harris and published by Springer Nature. This book was released on 2020-01-14 with total page 657 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book follows on from Volume 83 in the SCBI series (“Macromolecular Protein Complexes”), and addresses several important topics (such as the Proteasome, Anaphase Promoting Complex, Ribosome and Apoptosome) that were not previously included, together with a number of additional exciting topics in this rapidly expanding field of study. Although the first SCBI Protein Complex book focused on soluble protein complexes, the second (Vol. 87)addressed Membrane Complexes, and the third (Vol. 88) put the spotlight on Viral Protein and Nucleoprotein Complexes, a number of membrane, virus and even fibrillar protein complexes have been be considered for inclusion in the present book. A further book is also under preparation that follows the same pattern, in an attempt to provide a thorough coverage of the subject. Chapter 9 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Download or read book Bacterial Growth and Lysis written by M.A. de Pedro and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 467 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume is based on a FEMS Symposium entitled "Bacterial Growth and Lysis: Metabolism and Structure of the Bacterial Sacculus" held at the Monastery of Lluc (Mallorca, Spain) on 5-10 April, 1992. The goals of the symposium were to assess the present state of knowledge on the structure and physiology of the bacterial murein sacculus, and to develop new hypotheses and strategies to promote further development of the field. Consequently, the contributions compiled in this volume include broadly different approaches, from the introduction of new analytical methods to the presentation of provocative models for cell wall growth and division. Structural, biochemical, and genetic aspects are widely covered with special emphasis on the enzymology and regulation of murein hydrolases (autolysins). Comprehensive reviews on bacterial S layers and yeast cell walls are included to stimulate conceptual cross-feeding with these closely related topics. We believe that this book will provide the reader with a useful and up-to-date review of the topic. We would feel deeply rewarded by any positive influence this book may have on the future progress of the field, whereby all the scientific credit for it should be given to the authors of the excellent contributions presented.

Download or read book Antibiotic Inhibitors of Bacterial Cell Wall Biosynthesis written by Donald J. Tipper and published by Pergamon. This book was released on 1987 with total page 288 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the advent of antibiotic drugs, epidemic bacterial infections have ceased to be a scourge on mankind. This volume presents an overview of the field of antibiotic action. It also includes descriptions of the major mechanisms of resistance that are increasingly becoming a serious problem in dealing with bacterial infections. In detailing the precise mechanisms of action and resistance, allied to the rational application of modern organic chemistry, this information should highlight better structures for classical antibiotics, as well as other areas of bacterial metabolism that may be sensitive to chemotherapy. In such a multidisciplinary field, this accumulation of knowledge should be invaluable to all those working on better antibiotics.

Download or read book Antimicrobial Drug Resistance written by Douglas Mayers and published by Springer Science & Business Media. This book was released on 2009-07-06 with total page 673 pages. Available in PDF, EPUB and Kindle. Book excerpt: This ? rst edition of Antimicrobial Drug Resistance grew out of a desire by the editors and authors to have a comprehensive resource of information on antimicrobial drug resistance that encompassed the current information available for bacteria, fungi, protozoa and viruses. We believe that this information will be of value to clinicians, epidemiologists, microbiologists, virologists, parasitologists, public health authorities, medical students and fellows in training. We have endeavored to provide this information in a style which would be accessible to the broad community of persons who are concerned with the impact of drug resistance in our cl- ics and across the broader global communities. Antimicrobial Drug Resistance is divided into Volume 1 which has sections covering a general overview of drug resistance and mechanisms of drug resistance ? rst for classes of drugs and then by individual microbial agents including bacteria, fungi, protozoa and viruses. Volume 2 addresses clinical, epidemiologic and public health aspects of drug resistance along with an overview of the conduct and interpretation of speci? c drug resistance assays. Together, these two volumes offer a comprehensive source of information on drug resistance issues by the experts in each topic.

Download or read book Cambridge Scientific Biochemistry Abstracts written by and published by . This book was released on 1993-07 with total page 1498 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Atlas of Oral Microbiology From Healthy Microflora to Disease written by Xuedong Zhou and published by Springer Nature. This book was released on 2021-01-06 with total page 368 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is the second edition of Atlas of Oral Microbiology: From Healthy Microflora to Disease (ISBN 978-0-12-802234-4), with two new features: we add about 60 pictures of 14 newly isolated microbes from human dental plaque, at the same time, we re-organize the content of this book and provide more research progress about the oral microbiome bank of China, the invasion of oral microbiota into the gut, and the relationships between Oral Microflora and Human Diseases. This book is keeping up with the advanced edge of the international research field of oral microbiology. It innovatively gives us a complete description of the oral microbial systems according to different oral ecosystems. It collects a large number of oral microbial pictures, including cultural pictures, colonies photos, and electron microscopy photos. It is by far the most abundant oral microbiology atlas consists of the largest number of pictures. In the meantime, it also described in detail a variety of experimental techniques, including microbiological isolation, culture, and identification. It is an atlas with strong practical function. The editors and writers of this book have long been engaged in teaching and research work in oral microbiology and oral microecology. This book deserves a broad audience, and it will meet the needs of researchers, clinicians, teachers, and students major in biology, dental medicine, basic medicine, or clinical medicine. It can also be used to facilitate teaching and international academic exchanges.

Download or read book Practical Handbook of Microbiology written by Lorrence H Green and published by CRC Press. This book was released on 2021-05-04 with total page 2032 pages. Available in PDF, EPUB and Kindle. Book excerpt: Practical Handbook of Microbiology, 4th edition provides basic, clear and concise knowledge and practical information about working with microorganisms. Useful to anyone interested in microbes, the book is intended to especially benefit four groups: trained microbiologists working within one specific area of microbiology; people with training in other disciplines, and use microorganisms as a tool or "chemical reagent"; business people evaluating investments in microbiology focused companies; and an emerging group, people in occupations and trades that might have limited training in microbiology, but who require specific practical information. Key Features Provides a comprehensive compendium of basic information on microorganisms—from classical microbiology to genomics. Includes coverage of disease-causing bacteria, bacterial viruses (phage), and the use of phage for treating diseases, and added coverage of extremophiles. Features comprehensive coverage of antimicrobial agents, including chapters on anti-fungals and anti-virals. Covers the Microbiome, gene editing with CRISPR, Parasites, Fungi, and Animal Viruses. Adds numerous chapters especially intended for professionals such as healthcare and industrial professionals, environmental scientists and ecologists, teachers, and businesspeople. Includes comprehensive survey table of Clinical, Commercial, and Research-Model bacteria. The Open Access version of this book, available at http://www.taylorfrancis.com, has been made available under a Creative Commons Attribution-Non Commercial-No Derivatives 4.0 license. Chapter 21, "Archaea," of this book is freely available as a downloadable Open Access PDF under a Creative Commons Attribution-Non Commercial-No Derivatives 4.0 license available at http://www.taylorfrancis.com See Emanuel Goldman's Open Access article: "Lamarck redux and other false arguments against SARS-CoV-2 vaccination," https://www.embopress.org/doi/full/10.15252/embr.202254675