Download or read book PUF 8 and MPK 1 Genetic and Chemical Control of Spermatocyte Dedifferentiation in Caenorhabditis Elegans written by Matthew A Gaddy and published by . This book was released on 2021 with total page 52 pages. Available in PDF, EPUB and Kindle. Book excerpt: Stem cells face a number of major fate decision during their development: the decision to self-renew or differentiate, and then whether to remain differentiated or dedifferentiate, as occurs in some oncogenesis. A regulatory network controlling these decisions is vital to the development of all multicellular organisms, including humans. Aberrant regulation can result in either loss of specific cell type or uncontrolled cell proliferation, leading to tumors. However, our understanding of how differentiated cell can be reverted to an undifferentiated state remains far more limited.Using the nematode C. elegans germline as a model system, we previously reported that PUF-8 (a PUF RNA-binding protein) and LIP-1 (a dual-specificity phosphatase) inhibit the formation of germline tumors via repressing the dedifferentiation of spermatocytes into mitotic cells (termed "spermatocyte dedifferentiation") at least in part by inhibiting MPK-1 (an ERK MAPK homolog) activation. To gain insight into the molecular competence for spermatocyte dedifferentiation, we compared the germline phenotypes between two competent mutants -- puf-8(q725); lip-1(zh15) with a high MPK-1 activity and puf-8(q725); fem-3(q20gf) with a low MPK-1 activity. puf-8(q725); lip-1(zh15) mutants developed germline tumors more aggressively than puf-8(q725); fem-3(q20gf) mutants at 25̊C with aging. This result suggests that MPK-1 activation is critical to induce the formation of germline tumors via spermatocyte dedifferentiation. This idea was confirmed by treatment of puf-8(q725); fem-3(q20gf) mutant worms with Resveratrol, which stimulates MPK-1 activation. Our results show that 100 mM RSV significantly induced the formation of germline tumors via spermatocyte dedifferentiation at 25̊C with aging. Therefore, we conclude that MPK-1 activation is required to promote the formation of germline tumors via spermatocyte dedifferentiation in the absence of PUF-8. Since PUF-8 and MPK-1 are broadly conserved, we therefore suggest that similar molecular mechanisms may control dedifferentiation-mediated tumorigenesis in other organisms, including humans.
Download or read book Transcriptional and Translational Regulation of Stem Cells written by Gary Hime and published by Springer Science & Business Media. This book was released on 2013-05-22 with total page 370 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume describes the latest findings on transcriptional and translational regulation of stem cells. Both transcriptional activators and repressors have been shown to be crucial for the maintenance of the stem cell state. A key element of stem cell maintenance is repression of differentiation factors or developmental genes – achieved transcriptionally, epigenetically by the Polycomb complex, and post-transcriptionally by RNA-binding proteins and microRNAs. This volume takes two approaches to this topic – (1) illustrating the general principles outlined above through a series of different stem cell examples – embryonic, iPS and adult stem cells, and (2) describing several molecular families that have been shown to have roles in regulation of multiple stem cell populations.
Download or read book The Denervated Muscle written by Ernest Gutmann and published by Springer. This book was released on 2013-12-20 with total page 499 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Ras Activity and Signaling written by Ignacio Rubio and published by Humana. This book was released on 2022-05-26 with total page 439 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume compiles new experimental approaches and concepts focusing mostly, but not solely, on ways to manipulate and regulate Ras activity and its downstream signaling output. Chapters detail standard methodologies, biochemical methods, Ras processing trafficking and localization, Ras signaling and inhibition, and in vivo models for studying Ras function. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Ras Activity and Signaling: Methods and Protocols aims to provide support and guidance to lab workers in their work on Ras GTPases and in the design of new projects requiring novel methodologies.
Download or read book C elegans written by Kevin Strange and published by Springer Science & Business Media. This book was released on 2008-02-05 with total page 292 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular biology has driven a powerful reductionist, or “molecule-c- tric,” approach to biological research in the last half of the 20th century. Red- tionism is the attempt to explain complex phenomena by defining the functional properties of the individual components of the system. Bloom (1) has referred to the post-genome sequencing era as the end of “naïve reductionism. ” Red- tionist methods will continue to be an essential element of all biological research efforts, but “naïve reductionism,” the belief that reductionism alone can lead to a complete understanding of living organisms, is not tenable. Organisms are clearly much more than the sum of their parts, and the behavior of complex physiological processes cannot be understood simply by knowing how the parts work in isolation. Systems biology has emerged in the wake of genome sequencing as the s- cessor to reductionism (2–5). The “systems” of systems biology are defined over a wide span of complexity ranging from two macromolecules that interact to carry out a specific task to whole organisms. Systems biology is integrative and seeks to understand and predict the behavior or “emergent” properties of complex, multicomponent biological processes. A systems-level characteri- tion of a biological process addresses the following three main questions: (1) What are the parts of the system (i. e.
Download or read book Black Mountain Breakdown written by Lee Smith and published by Penguin. This book was released on 2012-03-06 with total page 264 pages. Available in PDF, EPUB and Kindle. Book excerpt: Crystal Spangler lives in rural Appalachia. She's the apple of her mother's eye -- not yet beautiful, but she will be. She's the most popular girl at Black Rock High. She makes cheerleader, gets good grades, and is elected beauty queen. Crystal discovers God, goes to college, and falls in love. When she comes home, she's disheveled and confused. Crystal becomes a wealthy politician's wife. But there's something calling her, drawing her back to where it all began, in the shadow of Black Mountain . . .
Download or read book Saint Monkey A Novel written by Jacinda Townsend and published by W. W. Norton & Company. This book was released on 2014-02-24 with total page 387 pages. Available in PDF, EPUB and Kindle. Book excerpt: "[A] compelling debut…Townsend's writing [is] full of fresh turns of phrase and keen insights." —Ayana Mathis, New York Times Book Review Fourteen-year-old Audrey Martin, with her Poindexter glasses and her head humming the 3/4 meter of gospel music, knows she’ll never get out of Kentucky—but when her fingers touch the piano keys, the whole church trembles. Her best friend, Caroline, daydreams about Hollywood stardom, but both girls feel destined to languish in a slow-moving stopover town in Montgomery County. That is, until chance intervenes and a booking agent offers Audrey a ticket to join the booming jazz scene in Harlem—an offer she can’t resist, not even for Caroline. And in New York City the music never stops. Audrey flirts with love and takes the stage at the Apollo, with its fast-dancing crowds and blinding lights. But fortunes can turn fast in the city—young talent means tough competition, and for Audrey failure is always one step away. Meanwhile, Caroline sinks into the quiet anguish of a Black woman in a backwards country, where her ambitions and desires only slip further out of reach. Jacinda Townsend’s remarkable first novel is a coming-of-age story made at once gripping and poignant by the wild energy of the Jazz Era and the stark realities of segregation. Marrying musical prose with lyric vernacular, Saint Monkey delivers a stirring portrait of American storytelling and marks the appearance of an auspicious new voice in literary fiction.
Download or read book Storming Heaven A Novel written by Denise Giardina and published by W. W. Norton & Company. This book was released on 2010-07-05 with total page 360 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the story of the miners and the union they wanted, of the people who loved them and the people who wanted to kill their dreams. Annadel, West Virginia, was a small town rich in coal, farms, and close-knit families, all destroyed when the coal company came in. It stole everything it hadn't bothered to buy—land deeds, private homes, and ultimately, the souls of its men and women. Four people tell this powerful, deeply moving tale: Activist Mayor C. J. Marcum. Fierce, loveless union man Rondal Lloyd. Gutsy nurse Carrie Bishop, who loved Rondal. And lonely, Sicilian immigrant Rosa Angelelli, who lost four sons to the deadly mines. They all bear witness to nearly forgotten events of history, culminating in the final, tragic Battle of Blair Mountain—when the United States Army greeted ten thousand unemployed pro-union miners with airplanes, bombs, and poison gas. It was the first crucial battle of a war that has yet to be won.
Download or read book The First Fifty Years 1913 1963 written by Arthur Andersen & Co and published by . This book was released on 1963 with total page 226 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Mountain Life and Work written by and published by . This book was released on 1984 with total page 348 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Pastor s Wife written by Elizabeth Von Arnim and published by . This book was released on 1915 with total page 534 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Building Cultural Competence written by Darla K. Deardorff and published by Taylor & Francis. This book was released on 2023-07-03 with total page 517 pages. Available in PDF, EPUB and Kindle. Book excerpt: For HR directors, corporate trainers, college administrators, diversity trainers and study abroad educators, this book provides a cutting-edge framework and an innovative collection of ready-to-use tools and activities to help build cultural competence—from the basics of understanding core concepts of culture to the complex work of negotiating identity and resolving cultural differences.Building Cultural Competence presents the latest work in the intercultural field and provides step-by-step instructions for how to effectively work with the new models, frameworks, and exercises for building learners’ cultural competence. Featuring fresh activities and tools from experienced coaches, trainers, and facilitators from around the globe, this collection of over 50 easy-to-use activities and models has been used successfully worldwide in settings that range from Fortune 500 corporations to the World Bank, non-profits, and universities. Learn updates on classic models like the DIE (Description, Interpretation, Evaluation) framework and the U-Curve model of adjustment. Engage in new exercises to help build intercultural competence, using the practical step-by-step guidance on how to effectively facilitate these activities. Stay relevant and have positive impact with clients, organizations, and students with these well-organized, easy-to-implement, and high impact collection of frameworks, models, and activities.The new, research-based models work for developing cultural competence in any environment, and for designing effective cultural competence courses. Education abroad administrators will be able to use these activities in their pre- departure orientations for students going abroad. Corporate human resource professionals will find these activities invaluable in cultural competence building programs.
Download or read book Cross Cultural Case Study written by Christopher Pole and published by JAI Press Incorporated. This book was released on 2000-12-20 with total page 200 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume is one of a series of research annuals on qualitative research designed to take up issues and debates in this area that relate to methodology, the relationship between data collection and data analysis, and other topics.
