Download or read book Protoplast Technology written by Kan Wang and published by Humana. This book was released on 2022-03-09 with total page 263 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed volume collects protocols from scientists who are actively engaged in developing or using protoplast technology. The book begins with chapters that focus on basic protoplast techniques and their utilities, such as protocols on protoplast isolation, transfection, and regeneration, as well as examples of how to use protoplasts for genome editing and gene function analysis in a number of major crop or model plant species. It continues by exploring protoplast automation, large scale functional genomics, and synthetic biology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Protoplast Technology: Methods and Protocols aims to inspire the new generation of researchers to further improve their protocols and apply this technology to accelerate the field of plant genomic study.

Download or read book Fungal Protoplasts written by J. Peberdy and published by CRC Press. This book was released on 1985-02-20 with total page 378 pages. Available in PDF, EPUB and Kindle. Book excerpt: Until now, information on fungal protoplasts has been scattered throughout various sources. With authoritative reviews of protoplast isolation and applications in fungal biology research, Fungal Protoplasts: Applications in Biochemistry and Genetics is the first volume devoted to a major area in experimental mycology-fungal protoplasts. Written by 18 pioneering experts, this unmatched, illustrated reference presents well-established knowledge of protoplast research as well as newer ideas and methods. The book encompasses advances in protoplast isolation techniques and methodology, uses of protoplasts in physiological, biochemical, and genetic studies, and developments in protoplast fusion that form the basis for transformation and gene cloning experiments, including applications in industrial biotechnology. This fact-filled book also features end-of-chapter bibliographies for further research.

Download or read book Tissue Culture of Trees written by John H. Dodds and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 157 pages. Available in PDF, EPUB and Kindle. Book excerpt: 1 John H. Dodds The culture offragmen ts of plant tissue is not a particularly new science, in fact as long ago as 1893 Rechinger (1893) described the formation of callus on isolated fragments of stems and roots. The culture of plant tissues in vitro on a nutrient medium was performed by Haberlandt (1902), however, his attempts were unsuccessful because he chose too simple a medium that lacked critical growth factors. Over the last fifty years there has been a surge of development in plant tissue culture techniques and a host of techniques are now avail able (Dodds and Roberts, 1982). The major areas are as follows. Callus Culture Callus is a rather ill-dermed material. but is usually described as an un organised proliferating mass of tissue. Although callus cultures have a great deal of potential in the biotechnological aspects of tissue culture, i.e. secondary product formation, they are not very suitable for plant propagation. The key reason for their unsuitability is that genetic aber rations occur during mitotic divisions in callus growth (D'amato.l965). The aberrations can be of a major type, such as aneuploidy or endo reduplication. It follows therefore that the genetic status of the re generated plants is different from that of the parent type. In general terms this genetic instability is undesirable, but there are occasions when a callus stage can be purposely included to diversify the genetic base of the crop.

Download or read book PLANT PROTOPLASTS written by Fowke and published by Boca Raton, Fla. : CRC Press. This book was released on 1985-10-04 with total page 264 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protoplast isolation and culture; Regeneration of plants; Protoplasts for studies of cell organelles; Protoplast fusion, Plant protoplasts and the cell cycle; Isolated protoplasts as laboratory tools in the study of cell wall deposition; Cytodifferentiation; The use of protoplasts for studies on membrane transport in plants; Protoplasts in virology; Plant protoplasts and genetic variation; Plant mutant isolation via protoplasts; Hybridization by somatic cell fusion; Plant protoplast transformation by Agrobacterium in relation to plant biotechnology.

Download or read book Plant Protoplasts and Genetic Engineering I written by Y. P. S. Bajaj and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 461 pages. Available in PDF, EPUB and Kindle. Book excerpt: Isolated protoplasts are a unique tool for genetic manipulation of plants. Since the discovery of a method for the enzymatic isolation of pro-· toplasts by Professor E. C. Cocking in 1960, tremendous progress has been made in this very fascinating area of research. I have witnessed the struggle in the 1960's and early 1970's, when obtaining a clean prepara tion of protoplasts was considered an achievement. I also shared the pioneering excitement and enthusiasm in this field during the 2nd Inter national Congress of Plant Tissue Culture held at Strasbourg in 1970, where Dr. I. Thkebe of Japan presented his work on the induction of division in tobacco protoplasts. This was followed by my participation in three international conferences devoted to plant protoplasts held in 1972 in Salamanca (Spain) and Versailles (France), and then in 1975 in Nottingham (England). The enthusiasm shown by plant scientists at these meetings was ample proof of the bright future of protoplast technology, and it became evident that protoplasts would playa major role in plant biotechnology, especially in genetic engineering. Since then we have never looked back, and now the methods for isolation, fusion, and culture, as well as regeneration of somatic hybrids, have become routine laboratory procedures for most plant species. Currently the focus is on cereal and tree protoplasts. In order to bring about any genetic manipulation through fusion, in corporation of DNA, and transformation, the regeneration of the entire plant through manipulation of protoplasts is a prerequisite.

Download or read book Plant Protoplasts written by Tessa Bengochea and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 97 pages. Available in PDF, EPUB and Kindle. Book excerpt: Isolated plant protoplasts are 'naked' cells that have had their cell wall removed either by mechanical action or by enzymic digestion. As a result of wall removal the only barrier that exists between the cell protoplasm and the external environment is the plasma membrane. The removal of the cell wall has drastic osmotic consequences for the isolated cell. Rather like animal cells, the isolated protoplasts must be maintained in an osmotically balanced (isotonic) nutrient medium or they will burst. As will be seen later, the isolation of plant protoplasts is not a particularly new event; as early as the 1890s scientists were isolating protoplasts mechanically and studying effects such as protoplast streaming. What has brought protoplast technology to the forefront of plant biology is the potential application of these structures in non-traditional plant improvement methods. Extreme care must be exercised by the reader, however, in be lieving some of the claims that have been put forward for the use of protoplasts in improvement programmes. In the 20 years since the first experiments in routine enzyme isolation of large numbers of protoplasts no real improvement has been obtained in a commercial crop plant. This does not mean that improvement is impossible; it simply indicates that it may be a longer term objective than some people would like to admit.

Download or read book Plant Protoplasts and Genetic Engineering V written by Professor Dr. Y. P. S. Bajaj and published by Springer Science & Business Media. This book was released on 2013-03-14 with total page 432 pages. Available in PDF, EPUB and Kindle. Book excerpt: In continuation of Volumes 8, 9, 22, and 23, this new volume deals with the regeneration of plants from isolated protoplasts and genetic transformation in various species of Actinidia, Allocasuarina, Anthurium, Antirrhinum, Asparagus, Beta, Brassica, Carica, Casuarina, Cyphomandra, Eucalyptus, Ipomoea, Larix, Limonium, Liriodendron, Malus, Musa, Physcomitrella, Physalis, Picea, Rosa, Tagetes, Triticum, and Ulmus. These studies reflect the far-reaching implications of protoplast technology in genetic engineering of plants. The book contains a wealth of useful information for advanced students, teachers, and researchers in the field of plant tissue culture, molecular biology, genetic engineering, plant breeding, and general biotechnology.

Download or read book Progress in Plant Protoplast Research written by K.J. Puite and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 415 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proceedings of the 7th International Protoplast Symposium, Wageningen, The Netherlands, December 6-11, 1987

Download or read book Plant Protoplasts written by Fowke and published by CRC Press. This book was released on 2018-01-18 with total page 254 pages. Available in PDF, EPUB and Kindle. Book excerpt: The present book thus emphasizes the importance of plant protoplasts for fundamental research. This book is intended to be used b senior undergraduates, graduate students, and research scientists in plant biology.

Download or read book Plant Protoplasts and Genetic Engineering IV written by Y. P. S. Bajaj and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 406 pages. Available in PDF, EPUB and Kindle. Book excerpt: In continuation of Volumes 8, 9, and 22 on in vitro manipulation of plant protplasts, this new volume deals with the regeneration of plants from protoplasts and genetic transformation in various species of Actinidia, Amoracia, Beta, Brassica, Cicer, Citrus, Cucumis, Duboisia, Fragaria, Glycine, Ipomoea, Lactuca, Lotus, Lycopersicon, Manihot, Medicago, Nicotiana, Petunia, Phaseolus, Pisum, Prunus, Psophocarpus, Saccharum, Solanum, Sorghum, Stylosanthes, and Vitis. These studies reflect the far-reaching implications of protoplast technology in genetic engineering of plants. They are of special interest to researchers in the field of plant tissue culture, molecular biology, genetic engineering, and plant breeding.

Download or read book Differentiation of Protoplasts and of Transformed Plant Cells written by J. Reinert and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 163 pages. Available in PDF, EPUB and Kindle. Book excerpt: H. BINDING and J. REINERT In collaboration with the first authors of this volume This volume is devoted to the development of cell clones and plants from ma nipulated cells: isolated protoplasts, cell fusion bodies, and transformed cells. Isolated protoplasts represent cells which are liberated from their walls and separated from the differentiation pattern of the organism. Investigations on re generation from protoplasts provide a better understanding of the process and control of developmental pathways. Whereas protoplast isolation results in alteration of the state of differentiation of a cell, protoplast fusion is a means for the creation of cells with novel genetic constitution. Fascinating features are (1) to hybridize cells which-unlike gametes -did not derive from meiosis products, (2) to bring together foreign plastids and mitochondria and to investigate their parasexual reactions, and (3) to match ge netic traits which had been separated for long periods of evolution. Highly sophisticated techniques have already been elaborated for the transfer of genes by the use of isolated DNA and gene transfer systems. Highly promising results have already been obtained by the use of Ti plasmids of Agrobacterium, but direct DNA transformation is also proving to be useful. Most of the results in these areas are preliminary and/or limited to a few sys tem.lt is the aim of this volume to present the main features, but at the same time to draw attention to problems and perspectives of protoplast regeneration and so matic cell genetics in order to stimulate further investigations.

Download or read book Plant Protoplasts and Genetic Engineering VII written by Y. P. S. Bajaj and published by Springer Science & Business Media. This book was released on 2013-03-14 with total page 339 pages. Available in PDF, EPUB and Kindle. Book excerpt: Twenty-seven chapters deal with the regeneration of plants from protoplasts and genetic transformation in various species of Agrostis, Allium, Anthriscus, Asparagus, Avena, Boehmeria, Carthamus, Coffea, Funaria, Geranium, Ginkgo, Gladiolus, Helianthus, Hordeum, Lilium, Lithospermum, Mentha, Panax, Papaver, Passiflora, Petunia, Physocomitrella, Pinus, Poa, Populus, Rubus, Saintpaulia, and Swertia. These studies reflect the far-reaching implications of protoplast technology in genetic engineering of plants. This volume is of special interest to advanced students, teachers, and research scientists in the field of plant tissue culture, molecular biology, genetic engineering, plant breeding, and general plant biotechnology.

Download or read book Plant Protoplasts and Genetic Engineering II written by Y. P. S. Bajaj and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 516 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Plant Protoplasts and Genetic Engineering VI written by Y.P.S Bajaj and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 329 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume comprising 28 chapters on the in vitro manipulation of plant protoplasts contributed by inter- national experts deals with the isolation, fusion, culture, immobilization, cryopreservation and ultrastructural studies on protoplasts and the regeneration of somatic hybrids and cybrids.

Download or read book Plant Protoplasts and Genetic Engineering III written by Y. P. S. Bajaj and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 349 pages. Available in PDF, EPUB and Kindle. Book excerpt: In continuation of Volumes 8 and 9 (1989) on in vitro manipulation of plant protoplasts, this new volume deals with the regeneration of plants from protoplasts and genetic transformation in various species of Agrostis, Arabidopsis, Atropa, Brassica, Catharanthus, Datura, Cucumis, Daucus, Digitalis, Duboisia, Eustoma, Festuca, Helianthus, Hordeum, Kalanchoe, Linum, Lobelia, Lolium, Lotus, Lycium, Lycopersicum, Mentha, Nicotiana, Pelargonium, Pisum, Pyrus, Salvia, Scopolia, and Solanum.These studies reflect the far reaching implications of protoplast technologyin genetic engineering of plants. They are of special interest to researchers in the field of plant tissue culture, molecular biology, genetic engineering, and plant breeding.

Download or read book Fungal Protoplasts written by John F. Peberdy and published by CRC Press. This book was released on 2020-10-28 with total page 369 pages. Available in PDF, EPUB and Kindle. Book excerpt: Until now, information on fungal protoplasts has been scattered throughout various sources. With authoritative reviews of protoplast isolation and applications in fungal biology research, Fungal Protoplasts: Applications in Biochemistry and Genetics is the first volume devoted to a major area in experimental mycology-fungal protoplasts. Written by 18 pioneering experts, this unmatched, illustrated reference presents well-established knowledge of protoplast research as well as newer ideas and methods. The book encompasses advances in protoplast isolation techniques and methodology, uses of protoplasts in physiological, biochemical, and genetic studies, and developments in protoplast fusion that form the basis for transformation and gene cloning experiments, including applications in industrial biotechnology. This fact-filled book also features end-of-chapter bibliographies for further research.

Download or read book The Physiological Properties of Plant Protoplasts written by Paul-Emile Pilet and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 296 pages. Available in PDF, EPUB and Kindle. Book excerpt: The idea for the p~esent book arose from a 3-day seminar which I organized in March 1984 for young research workers in plant physiology. Participants came from several universities of the French-speaking part of Switzerland and speakers from Basel, Mtinchen, Nottingham, Perpignan, Regensburg, Sheffield, Toulouse, Yale, ZUrich ... and Lausannne. The theme of the seminar was chosen from the range of research fields of our Institute. Meanwhile, feeling it was important to bear in mind that some of our hearers were not specialists in the chosen topic, I wished to pro vide a subject that would be of scientific, methodological and epistemologi cal interest. The critical analysis of the structural and functional characteristics of plant protoplasts exactly met these criteria. There exists ample material for discussion of the techniques of protoplast preparation, methods used in morphological, biological and biochemical studies, and for the comparison of protoplasts with the cells from which they are obtained.