Download or read book Protoplast Fusion written by Y. Y. Gleba and published by Springer. This book was released on 2011-12-27 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Although only about 3 years have passed since the preparation of the original manuscript of this book for the Russian edition, the number of successful experiments on somatic hybridization of higher plants has doubled. Although the main inferences of the first edition still remain in force, most of them have received conclusive experimental support and, moreover, some new con clusions have been drawn. It can be expected that these inferences and conclusions will constitute a more or less durable foundation for somatic cell genetics of higher plants. We thus hope this book will also remain useful over the next years, in spite of the rapid progress of experiments and the increase in the number of scientific reports in this field. Though it might appear strange to an uninvolved observer, the principal progress in hybridization of somatic cells of higher plants has been due to plant physiologists (who entered the field by elaborating methods and techniques for plant cell and, later, for isolated protoplast culture) rather than plant geneticists. However, further qualitative improvement in this field is inconceivable without the instillation of genetic ideology and the strict logic of genetic experiments. The main purpose ofthis book is the attempt to organize the available experimental data in terms and cate gories of genetic analysis. For this reason, this book lays no claim on being a comprehensive treatise on somatic hybridization.
Download or read book Plant Cell and Tissue Culture written by J. Reinert and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 85 pages. Available in PDF, EPUB and Kindle. Book excerpt: The techniques of plant organ, tissue, and cell culture concentrated on reproducibility, simplicity and accu are now established in many research laboratories racy with sufficient illustration to make all mani throughout the world and are being used in numerous pulations clear. areas of plant science. Methods have been developed The drawings of items used in the bench layout to propagate plants and free them from viruses using diagrams are symbolic and are 'keyed in' by number to shoot tip culture. The regeneration of plants from callus the list of materials and equipment. A line around an culture has also proved useful commercially. Elegant item indicates that is sterile. techniques have been used to synthesise somatic The adoption of an integrated text in which diagrams hybrids by the fusion of protoplasts and to transform are related spatially to the methods will, we hope, help cells. These and many other techniques have been the student to grasp the techniques quickly and effec and can be used to investigate a variety of botanical tively. This is first and foremost a manual which has its phenomena as well as to improve crop plants and now place on the laboratory bench open in front of the provide an important part of the basic experimental student, a book to be used! skills required by a majority of experimental botanists.
Download or read book PLANT PROTOPLASTS written by Fowke and published by Boca Raton, Fla. : CRC Press. This book was released on 1985-10-04 with total page 264 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protoplast isolation and culture; Regeneration of plants; Protoplasts for studies of cell organelles; Protoplast fusion, Plant protoplasts and the cell cycle; Isolated protoplasts as laboratory tools in the study of cell wall deposition; Cytodifferentiation; The use of protoplasts for studies on membrane transport in plants; Protoplasts in virology; Plant protoplasts and genetic variation; Plant mutant isolation via protoplasts; Hybridization by somatic cell fusion; Plant protoplast transformation by Agrobacterium in relation to plant biotechnology.
Download or read book Tissue Culture of Trees written by John H. Dodds and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 157 pages. Available in PDF, EPUB and Kindle. Book excerpt: 1 John H. Dodds The culture offragmen ts of plant tissue is not a particularly new science, in fact as long ago as 1893 Rechinger (1893) described the formation of callus on isolated fragments of stems and roots. The culture of plant tissues in vitro on a nutrient medium was performed by Haberlandt (1902), however, his attempts were unsuccessful because he chose too simple a medium that lacked critical growth factors. Over the last fifty years there has been a surge of development in plant tissue culture techniques and a host of techniques are now avail able (Dodds and Roberts, 1982). The major areas are as follows. Callus Culture Callus is a rather ill-dermed material. but is usually described as an un organised proliferating mass of tissue. Although callus cultures have a great deal of potential in the biotechnological aspects of tissue culture, i.e. secondary product formation, they are not very suitable for plant propagation. The key reason for their unsuitability is that genetic aber rations occur during mitotic divisions in callus growth (D'amato.l965). The aberrations can be of a major type, such as aneuploidy or endo reduplication. It follows therefore that the genetic status of the re generated plants is different from that of the parent type. In general terms this genetic instability is undesirable, but there are occasions when a callus stage can be purposely included to diversify the genetic base of the crop.
Download or read book Yeast Protocols written by Ivor Howell Evans and published by Springer. This book was released on 1996 with total page 433 pages. Available in PDF, EPUB and Kindle. Book excerpt: Yeast Protocols contains many key techniques for studying the biology of yeasts at both the cellular and molecular levels. Working primarily from Saccharomyces cerevisiae, the expert contributors explain step-by-step how to successfully isolate, identify, and culture yeasts; the secrets of meiotic mapping; how to use PFGE in karyotyping and gene localization; the methods for purification and analysis of various cell components; and the construction and exploitation of genomic DNA clone banks. They also cover the latest methods for chromosome engineering, insertional mutagenesis by Ty elements, mRNA abundance and half-life measurements, the use of reporter gene systems, genotoxicity testing, and more. Yeast Protocols follows the widely applauded Humana Methods in Molecular Biology style: brief introductions putting the particular method in context, comprehensive lists of materials, cookbook style instructions, and troubleshooting notes to avoid common pitfalls and solve problems. The techniques can be used with confidence and success by both inexperienced newcomers and established researchers.
Download or read book Fungal Protoplasts written by J. Peberdy and published by CRC Press. This book was released on 1985-02-20 with total page 378 pages. Available in PDF, EPUB and Kindle. Book excerpt: Until now, information on fungal protoplasts has been scattered throughout various sources. With authoritative reviews of protoplast isolation and applications in fungal biology research, Fungal Protoplasts: Applications in Biochemistry and Genetics is the first volume devoted to a major area in experimental mycology-fungal protoplasts. Written by 18 pioneering experts, this unmatched, illustrated reference presents well-established knowledge of protoplast research as well as newer ideas and methods. The book encompasses advances in protoplast isolation techniques and methodology, uses of protoplasts in physiological, biochemical, and genetic studies, and developments in protoplast fusion that form the basis for transformation and gene cloning experiments, including applications in industrial biotechnology. This fact-filled book also features end-of-chapter bibliographies for further research.
Download or read book Protoplast Fusion written by I︠U︡riĭ I︠U︡rʹevich Gleba and published by Springer. This book was released on 1984-06 with total page 240 pages. Available in PDF, EPUB and Kindle. Book excerpt: Techniques of parasexual hybridization, protoplast fusion and parasexual hybridization of higher plants, use of somatic hybridization.
Download or read book Plant Protoplasts written by Fowke and published by CRC Press. This book was released on 2018-01-18 with total page 254 pages. Available in PDF, EPUB and Kindle. Book excerpt: The present book thus emphasizes the importance of plant protoplasts for fundamental research. This book is intended to be used b senior undergraduates, graduate students, and research scientists in plant biology.
Download or read book Plant Protoplasts and Genetic Engineering I written by Y. P. S. Bajaj and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 461 pages. Available in PDF, EPUB and Kindle. Book excerpt: Isolated protoplasts are a unique tool for genetic manipulation of plants. Since the discovery of a method for the enzymatic isolation of pro-· toplasts by Professor E. C. Cocking in 1960, tremendous progress has been made in this very fascinating area of research. I have witnessed the struggle in the 1960's and early 1970's, when obtaining a clean prepara tion of protoplasts was considered an achievement. I also shared the pioneering excitement and enthusiasm in this field during the 2nd Inter national Congress of Plant Tissue Culture held at Strasbourg in 1970, where Dr. I. Thkebe of Japan presented his work on the induction of division in tobacco protoplasts. This was followed by my participation in three international conferences devoted to plant protoplasts held in 1972 in Salamanca (Spain) and Versailles (France), and then in 1975 in Nottingham (England). The enthusiasm shown by plant scientists at these meetings was ample proof of the bright future of protoplast technology, and it became evident that protoplasts would playa major role in plant biotechnology, especially in genetic engineering. Since then we have never looked back, and now the methods for isolation, fusion, and culture, as well as regeneration of somatic hybrids, have become routine laboratory procedures for most plant species. Currently the focus is on cereal and tree protoplasts. In order to bring about any genetic manipulation through fusion, in corporation of DNA, and transformation, the regeneration of the entire plant through manipulation of protoplasts is a prerequisite.
Download or read book Distant Hybridization of Crop Plants written by G. Kalloo and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 282 pages. Available in PDF, EPUB and Kindle. Book excerpt: Wild taxa are invaluable sources of resistance to diseases, insects/ pests, nematodes, temperature extremes, salinity and alkalinity stresses, and also of nutritional quality; adaptation; genetic diversity and new species. Utilization of wild relatives of a crop depends largely upon its crossability relations with cultivated varieties. Sev eral wild species are not crossable with the commercial cultivars due to various isolation barriers. Furthermore, in a few cases, hybridiza tion is possible only in one direction and reciprocal crosses are not successful, thus depriving the utilization of desired cytoplasm of many species. However, techniques have been developed to over come many barriers and hybrid plants are produced. New crop species have been developed by overcoming the F 1 sterility and producing amphidiploids and such crops are commercially being grown in the field. The segregation pattern ofF 1 hybrids produced by distant hybridization in segregating generations are different from the intervarietal hybrids. In former cases, generally, unidirectional segregation takes place in early generations and accordingly, selec tion procedures are adopted. In most of the cases, backcross or modified backcross methods have been followed to utilize wild species, and thus numerous types of resistance and other economical attributes have been transferred in the recurrent parents. Protoplast fusion has been amply demonstrated in a number of cases where sexual hybridization was not possible and, as a result, hybrids have been produced.
Download or read book Introduction to Plant Biotechnology written by H. S. Chawla and published by Science Publishers. This book was released on 2002 with total page 568 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant biotechnology has created unprecedented opportunities for the manipulation of biological systems of plants. To understand biotechnology, it is essential to know the basic aspects of genes and their organization in the genome of plant cells. This text on the subject is aimed at students.
Download or read book Protoplast Fusion written by Y. Y. Gleba and published by Springer. This book was released on 1984 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Although only about 3 years have passed since the preparation of the original manuscript of this book for the Russian edition, the number of successful experiments on somatic hybridization of higher plants has doubled. Although the main inferences of the first edition still remain in force, most of them have received conclusive experimental support and, moreover, some new con clusions have been drawn. It can be expected that these inferences and conclusions will constitute a more or less durable foundation for somatic cell genetics of higher plants. We thus hope this book will also remain useful over the next years, in spite of the rapid progress of experiments and the increase in the number of scientific reports in this field. Though it might appear strange to an uninvolved observer, the principal progress in hybridization of somatic cells of higher plants has been due to plant physiologists (who entered the field by elaborating methods and techniques for plant cell and, later, for isolated protoplast culture) rather than plant geneticists. However, further qualitative improvement in this field is inconceivable without the instillation of genetic ideology and the strict logic of genetic experiments. The main purpose ofthis book is the attempt to organize the available experimental data in terms and cate gories of genetic analysis. For this reason, this book lays no claim on being a comprehensive treatise on somatic hybridization.
Download or read book Primary and Secondary Metabolism of Plant Cell Cultures written by Karl-Hermann Neumann and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 381 pages. Available in PDF, EPUB and Kindle. Book excerpt: It must have been some feeling of frustration invoked by the genus loci of big conferences which brought Don Dougall, Mike Fowler, and me over a cup of coffee to think of organizing a small meeting on cell culture metabol ism. The basic aim was to bring people working on "primary metabolism" and those who work on "secondary metabolism" to interact with each other, hopefully to induce some new approaches to utilize the cell culture technique more efficiently in basic research and in its practical application. The out come of this was the small symposium at SchloE Rauischholzhausen near GieEen (Germany), and the reader of this small volume of articles on topics discussed will judge to what extent this aim was realized. Although F.e. Steward was unable to attend the meeting, we were happy that he contributed a review on some important turning points of the cell culture method in the past, and at the end of the book some participants of the meeting attempted some prognosis on possible future developments, summarized by Mike Fowler. It was the wish of our publisher to organize the contributions as a summary of the major topics of research of the individual groups represented at the meeting. Due to this, some readers will certainly feel that important areas of research in the cell culture field are missing. This is regrettable, but space had to be limited to keep this volume at a reasonable price.
Download or read book Somatic Hybridization in Crop Improvement II written by Toshiyuki Nagata and published by Springer Science & Business Media. This book was released on 2001-07-17 with total page 412 pages. Available in PDF, EPUB and Kindle. Book excerpt: Somatic hybrids through the fusion of plant protoplasts have widened the genetic variability of cultivated plants. As "Somatic Hybridization in Crop Improvement I", published in 1994, this volume describes how this discipline can contribute to the improvement of crops. It comprises 24 chapters dealing with interspecific and intergeneric somatic hybridization and cybridization. It is divided into four sections: I. Cereals: Barley, rice, and wheat. II. Vegetables and Fruits: Arabidopsis, Asparagus, Brassica, chicory, Citrus, Cucumis, Diospyros, Ipomoea, and various Solanaceous species, e.g., tomato, potato, and eggplant. III. Medicinal and Aromatic Plants: Atropa, Dianthus, Nicotiana, and Senecio. IV. Legumes/Pasture Crops: Alfalfa. This book is tailored to the needs of advanced students, teachers and researchers in the fields of plant breeding, genetic engineering, and plant tissue culture.
Download or read book Differentiation of Protoplasts and of Transformed Plant Cells written by J. Reinert and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 163 pages. Available in PDF, EPUB and Kindle. Book excerpt: H. BINDING and J. REINERT In collaboration with the first authors of this volume This volume is devoted to the development of cell clones and plants from ma nipulated cells: isolated protoplasts, cell fusion bodies, and transformed cells. Isolated protoplasts represent cells which are liberated from their walls and separated from the differentiation pattern of the organism. Investigations on re generation from protoplasts provide a better understanding of the process and control of developmental pathways. Whereas protoplast isolation results in alteration of the state of differentiation of a cell, protoplast fusion is a means for the creation of cells with novel genetic constitution. Fascinating features are (1) to hybridize cells which-unlike gametes -did not derive from meiosis products, (2) to bring together foreign plastids and mitochondria and to investigate their parasexual reactions, and (3) to match ge netic traits which had been separated for long periods of evolution. Highly sophisticated techniques have already been elaborated for the transfer of genes by the use of isolated DNA and gene transfer systems. Highly promising results have already been obtained by the use of Ti plasmids of Agrobacterium, but direct DNA transformation is also proving to be useful. Most of the results in these areas are preliminary and/or limited to a few sys tem.lt is the aim of this volume to present the main features, but at the same time to draw attention to problems and perspectives of protoplast regeneration and so matic cell genetics in order to stimulate further investigations.
Download or read book Plant Tissue Culture Manual written by K. Lindsey and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 138 pages. Available in PDF, EPUB and Kindle. Book excerpt: This manual comprises a range of techniques for research workers in the fields of cell and molecular biology, physiology, plant breeding and propagation, and genetic engineering.
Download or read book Fungal Protoplast written by D Lalithakumari and published by CRC Press. This book was released on 2019-04-24 with total page 198 pages. Available in PDF, EPUB and Kindle. Book excerpt: A detailed collection of the results obtained during the long history of the fungal protoplast work that has been published for different species. This overview is supplemented with research work into the improvement of biocontrol agents, carried out by the authors. Besides providing an overview of the literature, the book also acquaints one to pra
