Download or read book Protein Separation with Mathematical Modelling for Chromatographic Operation written by Hsien-Chih Ma and published by . This book was released on 1989 with total page 578 pages. Available in PDF, EPUB and Kindle. Book excerpt: We have performed experiments and derived mathematical models for packed bed columns used for liquid phase chromatographic separations of proteins with impulse input of feed solutions. These models can now be used to describe the relationships between the elution characteristics (peak height, peak position, and shapes) and the operating conditions (flow rate and buffer conditions) of ion exchange and gel permeation column chromatography for protein separations. The surface adsorption model was discussed relative to the nature of the mobile and stationary phases in ion exchange column chromatography for two distinct cases: with and without pore diffusion. For large solute molecules, such as proteins and enzymes, the surface adsorption model without pore diffusion is adequate for prediction of elution profiles from ion exchange columns. This model is shown to be sufficient, since the solute molecules cannot readily diffuse into the solid matrix of column packings. For smaller solute molecules, such as amino acids and peptides, one must consider both the pore diffusion in the solid matrix and the axial dispersion in the mobile phase. A separate gel permeation model for chromatography was developed to focus on the diffusion of solute molecules involving no adsorption on solid phase. The retention times of the large solute molecules are less than that of smaller molecules because of the lower probability for diffusion into the solid matrix of column packings. Thus, the application of a specific model depends on the origin of packing materials in the chromatography column, the size of the solute molecules, and the interactions between the solid and mobile phases. Effects of model parameters (column length, cross sectional area, flow rate, effective contact area, void fraction, particle size, axial dispersion, mass transfer coefficient, equilibrium constant, and pore diffusivity) on the calculated elution profiles are discussed based on the "series mass transfer mechanism". These effects are incorporated to describe the transport behaviors of solute molecules between the solid and liquid phases. The model protein system of hemoglobin and an albumin mixture was experimentally separated by cycling the change of pH in ion exchange column chromatography experiments, in order to study the transport relationship between the protein elution profile and transient pH wave. A pH phase lag within the column is needed to define for the pH cyclic zone operation in order to verify the elution characteristics between the experimental and predicted elution profiles. The success of our cycling techniques and models is further shown on the real protein system where we purified alkaline phosphatase from human placenta on an ion exchange packed bed with cycling of the buffer concentration. The optimal protein separation technique resulted in a high recovery and high purity product for this real protein enzyme system. The concentration phase lag and iso-ionic points are defined and combined with the relationships between the buffer concentrations and model parameters in order to predict the elution characteristics. The calculated and the experimental profiles are shown to be in good agreement when using the surface adsorption model without pore diffusion. The derived models can also be applied to determine the Number of Theoretical Plates (N) and Height Equivalent to Theoretical Plates (HETP) from the calculated profiles (peak height, peak width, retention time, and retention volume). The model parameters can be obtained from the limited experimental data for the desired operating conditions (mobile phase composition, flow rate, and column dimensions) in order to evaluate the column efficiency and optimization of column operation.

Download or read book Preparative Chromatography for Separation of Proteins written by Arne Staby and published by John Wiley & Sons. This book was released on 2017-02-02 with total page 608 pages. Available in PDF, EPUB and Kindle. Book excerpt: Preparative Chromatography for Separation of Proteins addresses a wide range of modeling, techniques, strategies, and case studies of industrial separation of proteins and peptides. • Covers broad aspects of preparative chromatography with a unique combination of academic and industrial perspectives • Presents Combines modeling with compliantce useing of Quality-by-Design (QbD) approaches including modeling • Features a variety of chromatographic case studies not readily accessible to the general public • Represents an essential reference resource for academic, industrial, and pharmaceutical researchers

Download or read book Towards Intensified Protein Refolding and Purification on Side Exclusion Chromatography written by Pegah Saremirad and published by . This book was released on 2015 with total page 220 pages. Available in PDF, EPUB and Kindle. Book excerpt: For industrial recombinant-protein processes, protein refolding and purification are crucial steps towards the recovery of considerable numbers of active and safe therapeutic products. In this thesis, intensification strategies for protein refolding and purification processes are explored. Development of an intensified process aims at simultaneous optimization of process performance indicators, namely: refolding yield, product purity, volumetric productivity and solvent consumption which in turn decrease the cost and time constraints to market. The first strategy investigated related to multivariable experimental work using size exclusion chromatography (SEC) as a refolding method and a denatured/reduced model protein (lysozyme). SEC was selected due to its potential for refolding of higher concentrations of protein compared to conventional refolding methods used currently in industry. The investigated variables were protein loading concentration, refolding buffer composition including pH, sodium chloride salt and ʟ-arginine, aggregation prevention additive, concentrations. The interplay of these process variables was studied and it was shown when ʟ-arginine is used, over the experimental space, the effects of pH and protein loading concentration on refolding yield are insignificant. This observation introduced the possibility of manipulating pH in a wider range without concerns for protein aggregation; for instance, to adjust the redox potential of the buffer without the need for costly redox couple chemicals to assist reformation of disulfide bridges in oxidative refolding of the protein. The results also provide more experimental evidence on the mechanism of aggregation prevention by ʟ-arginine. Secondly an experimentally-verified model of oxidative protein refolding on SEC was developed, with the goal of high-throughput process screening and optimization using the aforementioned model. Model development involved exploration of methods to find characteristic information on short-lived refolding kinetic species and lysozyme oxidative refolding kinetic schemes and constants under the two studied refolding environments, namely with and without ʟ-arginine additive. It was shown that ʟ-arginine prevents aggregation without considerable impact on the kinetics of lysozyme oxidative refolding. Finally, SEC in a multi-column continuous simulated moving bed configuration (SMB-SEC) was evaluated to fully exploit the potential of SEC for intensified protein refolding and purification. This configuration offers several advantages compared to single-column operation, including increased productivity per unit mass of solid phase, lower solvent consumption, and less diluted products, provided that operation parameters are screened and tuned for simultaneous optimization of process performance indicators. In this phase of the project, the effect of scale-up was predicted and considered for modifying and utilizing single-column model towards design/operation of a SMB-SEC. This thesis presents a framework for protein refolding and purification process development and optimization, including reduced cost of chemicals, improving the refolding yield, high-throughput measurements of parameters and finding a suitable reaction scheme of refolding and aggregation for mathematical model development applicable to both single-column and multi-column continuous operations, and defining appropriate process performance indicators for optimized operation of SMB-SEC.

Download or read book Separation Extraction and Concentration Processes in the Food Beverage and Nutraceutical Industries written by Syed S. H. Rizvi and published by Elsevier. This book was released on 2010-10-28 with total page 696 pages. Available in PDF, EPUB and Kindle. Book excerpt: Separation, extraction and concentration are essential processes in the preparation of key food ingredients. They play a vital role in the quality optimization of common foods and beverages and there is also increasing interest in their use for the production of high-value compounds, such as bioactive peptides from milk and whey, and the recovery of co-products from food processing wastes.Part one describes the latest advances in separation, extraction and concentration techniques, including supercritical fluid extraction, process chromatography and membrane technologies. It also reviews emerging techniques of particular interest, such as pervaporation and pressurised liquid extraction. Part two then focuses on advances in separation technologies and their applications in various sectors of the food, beverage and nutraceutical industries. Areas covered include dairy and egg processing, oilseed extraction, and brewing. This section discusses the characteristics of different foods and fluids, how food constituents are affected by separation processes and how separation processes can be designed and operated to optimize end product quality.With its team of experienced international contributors, Separation, extraction and concentration processes in the food, beverage and nutraceutical industries is an important reference source for professionals concerned with the development and optimisation of these processes. Describes the latest advances in separation, extraction and concentration techniques and their applications in various sectors of the food, beverage and nutraceutical industries Reviews emerging techniques of particular interest, such as pervaporation and pressurised liquid extraction Explores the characteristics of different foods and fluids and how food constituents are affected by separation processes

Download or read book Preparative Chromatography written by H. Schmidt-Traub and published by John Wiley & Sons. This book was released on 2012-09-27 with total page 545 pages. Available in PDF, EPUB and Kindle. Book excerpt: Completely revised and substantially extended to reflect the developments in this fast-changing field. It retains the interdisciplinary approach that elegantly combines the chemistry and engineering involved to describe the conception and improvement of chromatographic processes. It also covers recent advances in preparative chromatographic processes for the separation of "smaller" molecules using standard laboratory equipment as well as the detailed conception of industrial chemical plants. The increase in biopharmaceutical substances is reflected by new and revised chapters on different modifications of continuous chromatography as well as ion-exchange chromatography and other separation principles widely used in biochromatography. Following an introductory section on the history of chromatography, the current state of research and the design of chromatographic processes, the book goes on to define the general terminology. There then follow sections on stationary phases, selection of chromatographic systems and process concepts. A completely new chapter deals with engineering and operation of chromatographic equipment. Final chapters on modeling and determination of model parameters as well as model based design, optimization and control of preparative chromatographic processes allow for optimal selection of chromatographic processes. Essential for chemists and chemical engineers in the chemical, pharmaceutical, and food industries.

Download or read book European Symposium on Computer Aided Process Engineering 12 written by J. Grievink and published by Elsevier. This book was released on 2002-04-29 with total page 1059 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book contains 182 papers presented at the 12th Symposium of Computer Aided Process Engineering (ESCAPE-12), held in The Hague, The Netherlands, May 26-29, 2002. The objective of ESCAPE-12 is to highlight advances made in the development and use of computing methodologies and information technology in the area of Computer Aided Process Engineering and Process Systems Engineering. The Symposium addressed six themes: (1) Integrated Product&Process Design; (2) Process Synthesis & Plant Design; (3) Process Dynamics & Control; (4) Manufacturing & Process Operations; (5) Computational Technologies; (6) Sustainable CAPE Education and Careers for Chemical Engineers. These themes cover the traditional core activities of CAPE, and also some wider conceptual perspectives, such as the increasing interplay between product and process design arising from the often complex internal structures of modern products; the integration of production chains creating the network structure of the process industry and optimization over life span dimensions, taking sustainability as the ultimate driver.

Download or read book Mathematical Modeling and Scale Up of Liquid Chromatography written by Tingyue Gu and published by Springer. This book was released on 2015-04-06 with total page 214 pages. Available in PDF, EPUB and Kindle. Book excerpt: Tingyue Gu's second edition provides a comprehensive set of nonlinear multicomponent liquid chromatography (LC) models for various forms of LC, such as adsorption, size exclusion, ion-exchange, reversed-phase, affinity, isocratic/gradient elution and axial/radial flow LC. Much has advanced since the first edition of this book and the author's software, described here, is now used for teaching and research in 32 different countries. This book comes together with a complete software package with graphical user interface for personal computers, offered free for academic applications. Additionally, this book provides detailed methods for parameter estimation of mass transfer coefficients, bed voidage, particle porosity and isotherms. The author gives examples of how to use the software for predicitons and scale-up. In contrast to the first edition, authors do not need to deal with complicated math. Instead, they focus on how to obtain a few parameters for simulation and how to compare simulation results with experimental data. After reading the detailed descriptions in the book, a reader is able to use the simulation software to investigate chromatographic behavior without doing actual experiments. This book is aimed at readers who are interested in learning about LC behaviors and at those who want to scale up LC for preparative- and large-scale applications. Both academic personnel and industrial practitioners can benefit from the use of the book. This new edition includes: - New models and software for pellicular (cored) beads in liquid chromatography - Introduction of user-friendly software (with graphical user interface) - Detailed descriptions on how to use the software - Step-by-step instructions on parameter estimation for the models - New mass-transfer correlations for parameter estimation - Experimental methods for parameter estimation - Several actual examples using the model for product development and scale-up - Updated literature review

Download or read book Optimization of Ion exchange Chromatography of Proteins Using Mathematical Models written by Anna Johnston and published by . This book was released on 1991 with total page 546 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Separation Processes in the Food and Biotechnology Industries written by A S Grandison and published by Elsevier. This book was released on 1996-01-01 with total page 303 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book reviews methods and techniques for separating food components and products of the biotechnology industry. The introduction focuses on food composition and some of the conventional separation techniques. Subsequent chapters deal with each specific type or area of application individually and include information on the basic principles, industrial equipment available, commercial applications and an overview of research and development.

Download or read book Protein Chromatography written by Giorgio Carta and published by John Wiley & Sons. This book was released on 2020-06-02 with total page 440 pages. Available in PDF, EPUB and Kindle. Book excerpt: An all-in-one practical guide on how to efficiently use chromatographic separation methods Based on a training course that teaches the theoretical as well as practical aspects of protein bioseparation to bioprocess professionals, this fully updated and revised new edition offers comprehensive coverage of continuous chromatography and provides readers with many relevant examples from the biopharmaceutical industry. Divided into two large parts, Protein Chromatography: Process Development and Scale-Up, Second Edition presents all the necessary knowledge for effective process development in chromatographic bioseparation, both on small and large scale. The first part introduces chromatographic theory, including process design principles, to enable the reader to rationalize the set-up of a bioseparation process. The second part illustrates by way of case studies and sample protocols how the theory learned in the first part may be applied to real-life problems. Chapters look at: Downstream Processing of Biotechnology Products; Chromatography Media; Laboratory and Process Columns and Equipment; Adsorption Equilibrium; Rate Processes; and Dynamics of Chromatography Columns. The book closes with chapters on: Effects of Dispersion and Rate Processes on Column Performance; Gradient Elution Chromatography; and Chromatographic Column Design and Optimization. -Presents the most pertinent examples from the biopharmaceutical industry, including monoclonal antibodies -Provides an overview of the field along with design tools and examples illustrating the advantages of continuous processing in biopharmaceutical productions -Focuses on process development and large-scale bioseparation tasks, making it an ideal guide for the professional bioengineer in the biotech and pharma industries -Offers field-tested information based on decades of training courses for biotech and chemical engineers in Europe and the U.S. Protein Chromatography: Process Development and Scale-Up, Second Edition will appeal to biotechnologists, analytical chemists, chromatographers, chemical engineers, pharmaceutical industry, biotechnological industry, and biochemists.

Download or read book 19th European Symposium on Computer Aided Process Engineering written by Jacek Jezowski and published by Elsevier. This book was released on 2009-06-12 with total page 1341 pages. Available in PDF, EPUB and Kindle. Book excerpt: The 19th European Symposium on Computer Aided Process Engineering contains papers presented at the 19th European Symposium of Computer Aided Process Engineering (ESCAPE 19) held in Cracow, Poland, June 14-17, 2009.The ESCAPE series serves as a forum for scientists and engineers from academia and industry to discuss progress achieved in the area of CAPE. * CD-ROM that accompanies the book contains all research papers and contributions * International in scope with guest speeches and keynote talks from leaders in science and industry * Presents papers covering the latest research, key top areas and developments in computer aided process engineering (CAPE)

Download or read book Highly Selective Separations in Biotechnology written by G. Street and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 242 pages. Available in PDF, EPUB and Kindle. Book excerpt: Success in meeting the challenge to produce the commercial products anticipated by the exploitation of biological processes depends upon provid ing effective separation protocols. Effectiveness can be measured in terms of selectivity, purity, resolution and validatory success. The major processing problems are associated with either the selective recovery of molecules which are present in low concentrations from complex mixtures or the selective removal of contaminants from the desired molecule. Central to the evolution of processes satisfying this demand are the regulatory requirements being imposed by governments on the purity of a product, especially in the health care market. Synthetic organic chemists are increasingly finding it advantageous to conduct one or more steps using either enzymic biotransformations where molecules with a single and consistent stereochemistry or chirality are required. The underlying princi ples behind the methods, techniques and processes currently being used and developed commercially rely upon the biospecific nature and properties of the desired molecule. When these factors are married to the more traditional techniques of precipitation, chromatography, liquid-liquid extraction and membrane processes, powerful tools emerge, allowing highly selective separations to be designed. The logical extension of these combinations is to apply genetic engineering techniques to influence the separations at a more fundamental and structural level by modifying the target protein at source, during its synthesis, to facilitate its separation in a given, selective manner, leading to the distinct possibility of producing 'designer' separation programmes.

Download or read book Analytical and Preparative Separation Methods of Biomacromolecules written by Hassan Y. Aboul-Enein and published by CRC Press. This book was released on 2020-10-14 with total page 479 pages. Available in PDF, EPUB and Kindle. Book excerpt: Reports up-to-date research developments on purifying and isolation large organic molecules. The text provides information on high-performance liquid chromatography and capillary electrophoresis (CE) as tools for analyzing biomacromolecules and developing new biochemical and medicinal compounds. It applies biochemical separation technology to the study of macromolecules such as proteins, polysaccharides, nucleic acids and more.

Download or read book Preparative and Production Scale Chromatography written by G. Ganetsos and published by CRC Press. This book was released on 2019-11-11 with total page 812 pages. Available in PDF, EPUB and Kindle. Book excerpt: Describes the latest developments in the scaling-up and application of chromatographic operations and demonstrates that production-scale chromatography is a powerful and invaluable separation process. The book covers every important process design and reveals actual, immediately applicable techniques and is designed to appeal to design, chemical/biochemical, and research and development engineers, process development managers, bioprocess technologists, analytical and clinical chemists and biochemists, pharmacists, and upper-level undergraduate, graduate, and continuing-education students in these disciplines.

Download or read book Protein Purification Process Engineering written by Roger Harrison and published by Routledge. This book was released on 2019-07-16 with total page 377 pages. Available in PDF, EPUB and Kindle. Book excerpt: Offers coverage of the development of protein purification processes for large-scale commercial operations, and addresses process development, scale-up, applications and mathematical descriptions. Technologies currently used at the commercial scale are covered in depth.

Download or read book Purification of Biotechnological Products written by Adalberto Pessoa Jr and published by CRC Press. This book was released on 2024-05-23 with total page 539 pages. Available in PDF, EPUB and Kindle. Book excerpt: This outstanding text focuses on providing professionals and students working in the pharmaceutical and biotechnology field with the background necessary for developing of a product or process and with the necessary rigor required by federal regulatory agencies in the pharmaceutical industry. The material will enable teachers, lecturers and professors in biotechnology to prepare courses on basic concepts and applications for the purification of biotechnological products of industrial interest. These can be applied in practice, for example, with projects on purification development on an industrial scale or useful unit operations for the development of bioproducts of commercial interest. Features: Purification and development of new bioproducts and improvement of those being produced Provides a background and concepts on the purification of biomolecules and with an industrial perspective It allows professionals to understand the entire process of developing a biopharmaceutical or bio-food, from bench to industry in biotechnology; one of the fastest-growing sectors of the economy It promotes the dissemination of information in a didactic way which is of paramount importance for interdisciplinary fields It enables the reader to follow step-by-step stages of the development of a new biopharmaceutical, and allows the optimization of existing processes

Download or read book Comprehensive Biotechnology written by and published by Elsevier. This book was released on 2019-07-17 with total page 4876 pages. Available in PDF, EPUB and Kindle. Book excerpt: Comprehensive Biotechnology, Third Edition, Six Volume Set unifies, in a single source, a huge amount of information in this growing field. The book covers scientific fundamentals, along with engineering considerations and applications in industry, agriculture, medicine, the environment and socio-economics, including the related government regulatory overviews. This new edition builds on the solid basis provided by previous editions, incorporating all recent advances in the field since the second edition was published in 2011. Offers researchers a one-stop shop for information on the subject of biotechnology Provides in-depth treatment of relevant topics from recognized authorities, including the contributions of a Nobel laureate Presents the perspective of researchers in different fields, such as biochemistry, agriculture, engineering, biomedicine and environmental science