Download or read book Protein Refolding written by George Georgiou and published by . This book was released on 1991 with total page 248 pages. Available in PDF, EPUB and Kindle. Book excerpt: the refolding process is often the critical bottleneck in the production of high-value proteins, and recently acquired insights have yet to be translated into technological advantages. These proceedings bridge the gap between fundamental and applied studies, addressing such issues as in vivo protein folding, protein aggregation and inclusion body formation, elucidation of the folding pathway, characterization of folding intermediates, and practical considerations in protein renaturation. The symposium was part of the 199th ACS National Meeting, Boston, April 1990. Annotation copyrighted by Book News, Inc., Portland, OR
Download or read book Protein Folding written by C. M. Dobson and published by Cambridge University Press. This book was released on 1995 with total page 128 pages. Available in PDF, EPUB and Kindle. Book excerpt: Discusses the molecular mechanisms controlling protein folding in vivo and in vitro.
Download or read book Protein Folding written by Tony R. Obalinsky and published by Nova Publishers. This book was released on 2006 with total page 294 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proteins are one of the most basic components of all living cells and therefore serve a vital purpose in the cells of animals, plants and bacteria. They are comprised of chains of amino acids, which are held together by ribosome. These chains have many different patterns, which are known as `folds.' These folds are complicated, and therefore susceptible to irregularities that are known to be the source of many diseases. Cystic fibrosis, mad cow disease, Alzheimer's disease, emphysema and others are all initiated by improper protein folds. It is clear that, improving our understanding of protein folding is a key to fighting these diseases. This book presents recently performed research from around the world on this important subject.
Download or read book Protein Folding written by Victor Muñoz and published by Humana. This book was released on 2021-11-30 with total page 419 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides comprehensive protocols on experimental and computational methods that are used to study probe protein folding reactions and mechanisms. Chapters divided into five parts detail protein engineering, protein chemistry, experimental approaches to investigate the thermodynamics and kinetics of protein folding transitions, probe protein folding at the single molecule, analysis and interpretation of computer simulations, procedures and tools for the prediction of protein folding properties. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Protein Folding: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field.
Download or read book Protein Folding written by Alka Dwevedi and published by Springer. This book was released on 2014-12-01 with total page 61 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book will discuss classes of proteins and their folding, as well as the involvement of bioinformatics in solving the protein folding problem. In vivo and in vitro folding mechanisms are examined, as well as the failures of in vitro folding, a mechanism helpful in understanding disease caused by misfolding. The role of energy landscapes is also discussed and the computational approaches to these landscapes.
Download or read book Protein Stability and Folding written by Bret A. Shirley and published by . This book was released on 1995 with total page 377 pages. Available in PDF, EPUB and Kindle. Book excerpt: In Protein Stability and Folding: Theory and Practice, world-class scientists present in a single volume a comprehensive selection of hands-on recipes for all of the major techniques needed to understand the conformational stability of proteins, as well as their three-dimensional folding. The distinguished contributors provide clear, step-by-step instructions along with many troubleshooting tips, alternative procedures, and informative explanations about why certain steps are necessary. Even highly skilled researchers will find many time-saving methods. Among the techniques discussed are fluorescent, ultraviolet, and infrared spectroscopy; HPLC peptide mapping; differential scanning calorimetry; and hydrogen exchange. Shirley's Protein Stability and Folding: Theory and Practice will ensure a significant difference in the outcome of your experiments, producing the result desired even for beginners.
Download or read book Bioseparations of Proteins written by Ajit Sadana and published by Elsevier. This book was released on 1997-11-27 with total page 367 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book covers the fundamentals of protein inactivation during bioseparation and the effect on protein processing. Bioseparation of Proteins is unique because it provides a background of the bioseparation processes, and it is the first book available to emphasize the influence of the different bioseparation processes on protein inactivation.Bioseparation of Proteins covers the extent, mechanisms of, and control of protein inactivation during these processes along with the subsequent and essential validation of these processes. The book focuses on the avoidance of protein (biologicalproduct) inactivation at each step in a bioprocess. It compares protein inactivation exhibited during the different bioseparation processes by different workers and provides a valuable framework for workers in different areas interested in bioseparations.Topics include separation and detection methods; estimates of protein inactivation and an analysis of this problem for different separation processes; strategies for avoiding inactivation; the molecular basis of surface activity and protein adsorption,process monitoring, and product validation techniques; and the economics of various bioseparation processes and quality control procedures.Key Features* Protein inactivation and other aspects of biological stability are critical to an effective bioseparation process; This book is a detailed and critical review of the available literature in an area that is essential to the effectiveness, validation, and economics of bioseparation processes for drugs and other biological products; Conveniently assembled under one cover, the survey of the literature and resulting perspective will greatly assist engineers and chemists in designingand improving their own processes; Key features of the text include:* detailed data on biological stability under various bioseparation conditions* extensive case studies from the literature on separation processes, validation, and economics* simplified analysis of protein refolding and inactivation mechanisms* consideration of adsorption theories and the effect of heterogeneity* coverage of both classical and novel bioseparation techniques, including chromatographic procedures
Download or read book Protein Refolding Using Molecular Assistants written by Peter Eric Hanson and published by . This book was released on 1997 with total page 526 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Guide to Protein Purification written by Richard R Burgess and published by Academic Press. This book was released on 2009-11-03 with total page 915 pages. Available in PDF, EPUB and Kindle. Book excerpt: Guide to Protein Purification, Second Edition provides a complete update to existing methods in the field, reflecting the enormous advances made in the last two decades. In particular, proteomics, mass spectrometry, and DNA technology have revolutionized the field since the first edition's publication but through all of the advancements, the purification of proteins is still an indispensable first step in understanding their function. This volume examines the most reliable, robust methods for researchers in biochemistry, molecular and cell biology, genetics, pharmacology and biotechnology and sets a standard for best practices in the field. It relates how these traditional and new cutting-edge methods connect to the explosive advancements in the field. This "Guide to" gives imminently practical advice to avoid costly mistakes in choosing a method and brings in perspective from the premier researchers while presents a comprehensive overview of the field today. - Gathers top global authors from industry, medicine, and research fields across a wide variety of disciplines, including biochemistry, genetics, oncology, pharmacology, dermatology and immunology - Assembles chapters on both common and less common relevant techniques - Provides robust methods as well as an analysis of the advancements in the field that, for an individual investigator, can be a demanding and time-consuming process
Download or read book Oxidative Folding of Proteins written by Matthias J Feige and published by Royal Society of Chemistry. This book was released on 2018-07-27 with total page 450 pages. Available in PDF, EPUB and Kindle. Book excerpt: The formation of disulphide bonds is probably the most influential modification of proteins. These bonds are unique among post-translational modifications of proteins as they can covalently link cysteine residues far apart in the primary sequence of a protein. This has the potential to convey stability to otherwise marginally stable structures of proteins. However, the reactivity of cysteines comes at a price: the potential to form incorrect disulphide bonds, interfere with folding, or even cause aggregation. An elaborate set of cellular machinery exists to catalyze and guide this process: facilitating bond formation, inhibiting unwanted pairings and scrutinizing the outcomes. Only in recent years has it become clear how intimately connected this cellular machinery is with protein folding helpers, organellar redox balance and cellular homeostasis as a whole. This book comprehensively covers the basic principles of disulphide bond formation in proteins and describes the enzymes involved in the correct oxidative folding of cysteine-containing proteins. The biotechnological and pharmaceutical relevance of proteins, their variants and synthetic replicates is continuously increasing. Consequently this book is an invaluable resource for protein chemists involved in realted research and production.
Download or read book Biophysics And Biochemistry Of Protein Aggregation Experimental And Theoretical Studies On Folding Misfolding And Self assembly Of Amyloidogenic Peptides written by Jian-min Yuan and published by World Scientific. This book was released on 2017-06-02 with total page 327 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book reviews current research on the important processes involved in neurodegenerative diseases (e.g. Alzheimer's disease) and the peptides and proteins involved in the amyloidogenic processes. It covers the design and developments of anti-amyloid inhibitors, and gives readers a fundamental understanding of the underlying oligomerization and aggregation processes of these diseases from both computational and experimental points of view.
Download or read book Molecular Cell Biology written by Harvey F. Lodish and published by Macmillan. This book was released on 2008 with total page 1370 pages. Available in PDF, EPUB and Kindle. Book excerpt: The sixth edition provides an authoritative and comprehensive vision of molecular biology today. It presents developments in cell birth, lineage and death, expanded coverage of signaling systems and of metabolism and movement of lipids.
Download or read book Folding of Disulfide Proteins written by Rowen J. Y. Chang and published by Springer Science & Business Media. This book was released on 2011-08-12 with total page 290 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book aims to cover the knowledge of protein folding accumulated from studies of disulfide-containing proteins, including methodologies, folding pathways, and folding mechanism of numerous extensively characterized disulfide proteins. Folding of Disulfide Proteins will be valuable supplementary reading for general biochemistry, biophysics, molecular biology, and cellular biology courses for graduate and undergraduate students. This book can also be used for specialized graduate-level biochemistry, biophysics, and molecular biology courses dedicated to protein folding as well as related biological problems and diseases. Will also be of interest to everybody interested in problems related to protein folding, and anyone who is interested in understanding the mechanism of protein misfolding and protein misfolding-related diseases.
Download or read book Protein Folding Kinetics written by Bengt Nölting and published by Springer Science & Business Media. This book was released on 2013-03-09 with total page 197 pages. Available in PDF, EPUB and Kindle. Book excerpt: First methods book which includes many detailed descriptions Absolutely needed and thus timely for the scientific community Comprises 15% more content and includes the mentioned special features
Download or read book Protein Folding Evolution and Design written by Eugene I. Shakhnovich and published by IOS Press. This book was released on 2001 with total page 364 pages. Available in PDF, EPUB and Kindle. Book excerpt: This text presents the results of broad, interdisciplinary effort to study proteins in physical and evolutionary perpective. Among authors are physicists, computational, chemists, crystallographers and evolutionary biologists. Experimental and theoretical developments from molecules to cells are presented, providing a broad picture of modern biophysical chemistry.
Download or read book Handbook of Biogeneric Therapeutic Proteins written by Sarfaraz K. Niazi and published by CRC Press. This book was released on 2002-08-15 with total page 592 pages. Available in PDF, EPUB and Kindle. Book excerpt: More than 20 billion dollars worth of biopharmaceuticals are scheduled to go off-patent by 2006. Given the strong political impetus and the development of technological tools that can answer the questions regulatory authorities may raise, it is inevitable that the FDA and EMEA will allow biogeneric or biosimilar products. Even with all the regulato
Download or read book Protein Chromatography written by Sinéad T Loughran and published by Springer Nature. This book was released on 2023-10-01 with total page 495 pages. Available in PDF, EPUB and Kindle. Book excerpt: This third edition expands on the previous editions with updated and new chapters on protein chromatography. Chapters detail protein stability and storage, avoiding proteolysis, protein quantitation methods, generation and purification of recombinant proteins, recombinant antibody production, and the tagging of proteins. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Protein Chromatography: Methods and Protocols, Third Edition aims to provide commonly used methods and new approaches to help both new researchers and experts expand their knowledge.
