Download or read book Protein Purification Using Expanded Bed Chromatography written by and published by . This book was released on 2003 with total page 86 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Expanded Bed Chromatography written by B. Mattiasson and published by Springer Science & Business Media. This book was released on 2013-04-17 with total page 258 pages. Available in PDF, EPUB and Kindle. Book excerpt: Expanded bed adsorption chromatography is a novel processing technique for the purification of biomolecules, combining clarification, concentration and initial purification in one step. By such an integration it is possible to reduce the number of steps in the purification process, to shorten the processing time and to improve the yields. The technology is new, and interesting developments have taken place concerning the adsorbents, the processing technology and potential applications. Both small-scale laboratory processes and larger industrial processes are being developed. Expanded bed chromatography is one of the most exciting new developments in downstream processing in recent years. The technology will be a standard procedure when new biotechnological processes are being developed.
Download or read book Expanded Bed Adsorption Chromatography written by Norhafizah Abdullah and published by LAP Lambert Academic Publishing. This book was released on 2009 with total page 272 pages. Available in PDF, EPUB and Kindle. Book excerpt: Purifying protein is often a complicated task. This is due to protein s heterogeneity, complexity and instability when not in their native environment. It is the goal of this book to assemble the most generic approach for protein purification. The use of an expanded bed adsorption (EBA) chromatography as an integrative product capture and concentration technique allows single step purification of biomolecules from complex feedstock a possible alternative. There are a number of different approaches that can be taken in the choice and design of an appropriate adsorbent for use in EBA procedure. In this book, experimental investigation was focused on the choice of the ligand to be used on the solid phase adsorbent to ensure selective isolation of the target protein from a complex feedstock. True biospecific affinity, immobilized metal affinity chelating and ion exchange strategies were investigated for EBA purification. Recombinant glutathione-S-transferase (GST) expressed intracellularly in E.coli was chosen as a model protein. The book is aimed at researchers working in the area of protein purification.
Download or read book Isolation and Purification of Proteins written by Rajni Hatti-Kaul and published by CRC Press. This book was released on 2003-02-05 with total page 688 pages. Available in PDF, EPUB and Kindle. Book excerpt: This publication details the isolation of proteins from biological materials, techniques for solid-liquid separation, concentration, crystallization, chromatography, scale-up, process monitoring, product formulation, and regulatory and commercial considerations in protein production. The authors discuss the release of protein from a biological host, selectivity in affinity chromatography, precipitation of proteins (both non-specific and specific), extraction for rapid protein isolation, adsorption as an initial step for the capture of proteins, scale-up and commercial production of recombinant proteins, and process monitoring in downstream processing.
Download or read book Expanded Bed Adsorption Chromatography for Protein Purification written by Janus Tingleff Søndergaard and published by . This book was released on 2014 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Protein Purification Techniques written by Simon Roe and published by OUP Oxford. This book was released on 2001-01-25 with total page 279 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proteins are an integral part of molecular and cellular structure and function and are probably the most purified type of biological molecule. In order to elucidate the structure and function of any protein it is first necessary to purify it. Protein purification techniques have evolved over the past ten years with improvements in equipment control, automation, and separation materials, and the introduction of new techniques such as affinity membranes and expanded beds. These developments have reduced the workload involved in protein purification, but there is still a need to consider how unit operations linked together to form a purification strategy, which can be scaled up if necessary. The two Practical Approach books on protein purification have therefore been thoroughly updated and rewritten where necessary. The core of both books is the provision of detailed practical guidelines aimed particularly at laboratory scale purification. Information on scale-up considerations is given where appropriate. The books are not comprehensive but do cover the major laboratory techniques and common sources of protein. Protein Purification Techniques focuses on unit operations and analytical techniques. It starts with an overview of purification strategy and then covers initial extraction and clarification techniques. The rest of the book concentrates on different purification methods with the emphasis being on chromatography. The final chapter considers general scale-up considerations. Protein Purification Applications describes purification strategies from common sources: mammalian cell culture, microbial cell culture, milk, animal tissue, and plant tissue. It also includes chapters on purification of inclusion bodies, fusion proteins, and purification for crystallography. A purification strategy that can produce a highly pure single protein from a crude mixture of proteins, carbohydrates, lipids, and cell debris to is a work of art to be admired. These books (available individually or as a set)are designed to give the laboratory worker the information needed to undertake the challenge of designing such a strategy.
Download or read book Protein Purification Applications written by Simon Roe and published by OUP Oxford. This book was released on 2001-01-25 with total page 182 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proteins are an integral part of molecular and cellular structure and function and are probably the most purified type of biological molecule. In order to elucidate the structure and function of any protein it is first necessary to purify it. Protein purification techniques have evolved over the past ten years with improvements in equipment control, automation, and separation materials, and the introduction of new techniques such as affinity membranes and expanded beds. These developments have reduced the workload involved in protein purification, but there is still a need to consider how unit operations linked together to form a purification strategy, which can be scaled up if necessary. The two Practical Approach books on protein purification have therefore been thoroughly updated and rewritten where necessary. The core of both books is the provision of detailed practical guidelines aimed particularly at laboratory scale purification. Information on scale-up considerations is given where appropriate. The books are not comprehensive but do cover the major laboratory techniques and common sources of protein. Protein Purification Techniques focuses on unit operations and analytical techniques. It starts with an overview of purification strategy and then covers initial extraction and clarification techniques. The rest of the book concentrates on different purification methods with the emphasis being on chromatography. The final chapter considers general scale-up considerations. Protein Purification Applications describes purification strategies from common sources: mammalian cell culture, microbial cell culture, milk, animal tissue, and plant tissue. It also includes chapters on purification of inclusion bodies, fusion proteins, and purification for crystallography. A purification strategy that can produce a highly pure single protein from a crude mixture of proteins, carbohydrates, lipids, and cell debris to is a work of art to be admired. These books (available individually or as a set)are designed to give the laboratory worker the information needed to undertake the challenge of designing such a strategy.
Download or read book Methods for Affinity Based Separations of Enzymes and Proteins written by Munishwar N. Gupta and published by Birkhäuser. This book was released on 2013-12-01 with total page 234 pages. Available in PDF, EPUB and Kindle. Book excerpt: One major concern of biotechnology is either using enzymes or producing them. Enzyme/protein production is therefore an important starting point for biotechnology. Bioseparation or Downstream Processing constitutes about 40-90% of the total production cost. Driven by economics, highly selective technologies applicable to large-scale processing have emerged during the last decade. These technologies are slowly diffusing to enzymologists who are working on a smaller scale, looking for fast and efficient purification protocols. The affinity-based techniques (including precipitation, two-phase extractions, expanded bed chromatography, perfusion chromatography and monoliths) described in this volume provide current and new cutting-edge methods. Consequently, the book is of main interest to researchers in biochemistry, biochemical engineering and biotechnology, working either in academic or industrial sectors.
Download or read book Protein Purification written by R.K. Scopes and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 287 pages. Available in PDF, EPUB and Kindle. Book excerpt: New textbooks at all levels of chemistry appear with great regularity. Some fields like basic biochemistry, organic reaction mechanisms, and chemical ther modynamics are well represented by many excellent texts, and new or revised editions are published sufficiently often to keep up with progress in research. However, some areas of chemistry, especially many of those taught at the grad uate level, suffer from a real lack of up-to-date textbooks. The most serious needs occur in fields that are rapidly changing. Textbooks in these subjects usually have to be written by scientists actually involved in the research which is advancing the field. It is not often easy to persuade such individuals to set time aside to help spread the knowledge they have accumulated. Our goal, in this series, is to pinpoint areas of chemistry where recent progress has outpaced what is covered in any available textbooks, and then seek out and persuade experts in these fields to produce relatively concise but instructive introductions to their fields. These should serve the needs of one semester or one quarter graduate courses in chemistry and biochemistry. In some cases the availability of texts in active research areas should help stimulate the creation of new courses. New York CHARLES R.
Download or read book Protein Purification written by Jan-Christer Janson and published by John Wiley & Sons. This book was released on 1998 with total page 712 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is a state-of-the-art sourcebook on modern high-resolution biochemical separation techniques for proteins. It contains all the basic theory and principles used in protein chromatography and electrophoresis.
Download or read book Protein Purification Protocols written by Paul Cutler and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 474 pages. Available in PDF, EPUB and Kindle. Book excerpt: The first edition of Protein Purification Protocols (1996), edited by Professor Shawn Doonan, rapidly became very successful. Professor Doonan achieved his aims of p- ducing a list of protocols that were invaluable to newcomers in protein purification and of significant benefit to established practitioners. Each chapter was written by an ex- rienced expert in the field. In the intervening time, a number of advances have w- ranted a second edition. However, in attempting to encompass the recent developments in several areas, the intention has been to expand on the original format, retaining the concepts that made the initial edition so successful. This is reflected in the structure of this second edition. I am indebted to Professor Doonan for his involvement in this new edition and the continuity that this brings. Each chapter that appeared in the original volume has been reviewed and updated to reflect advances and bring the topic into the 21st century. In many cases, this reflects new applications or new matrices available from vendors. Many of these have increased the performance and/or scope of the given method. Several new chapters have been introduced, including chapters on all the currently used protein fractionation and ch- matographic techniques. They introduce the theory and background for each method, providing lists of the equipment and reagents required for their successful execution, as well as a detailed description of how each is performed.
Download or read book Simulated Moving Bed Technology written by Alirio Rodrigues and published by Butterworth-Heinemann. This book was released on 2015-05-23 with total page 305 pages. Available in PDF, EPUB and Kindle. Book excerpt: Simulated Moving Bed Technology is one of only a few books solely dedicated to SMB. It aims to equip you with the tools and skills needed for SMB modelling, simulation, design and operation. The theory presented is supported by real-world examples from the petrochemical, sugar and pharma industries.The core of the book focuses on SMB process development and model validation. It explains basic process concepts and definitions, fundamental principles and equations, as well as typical applications and recent advances made to address challenges. Its specialist content makes it a must have book for people working with SMB technology, chemical engineering post-graduate students and process and R&D engineers in industry. - Will provide you with the tools and predictive approaches for use in SMB design and performance assessment - Covers SMB modelling, simulation, design and operation as applied to separation and reaction processes - Discusses process intensification through coupling SMB and reaction in SMBR, membrane permeation and SMBR in PermSMBR and introduces the design of SCPC
Download or read book Downstream Processing of Proteins written by Mohamed A. Desai and published by Springer Science & Business Media. This book was released on 2008-02-05 with total page 233 pages. Available in PDF, EPUB and Kindle. Book excerpt: Considerable effort and time is allocated to introducing cell culture and fermentation technology to undergraduate students in academia, generally through a range of courses in industrial biotechnology and related disciplines. Similarly, a large number of textbooks are available to describe the appli- tions of these technologies in industry. However, there has been a general lack of appreciation of the significant developments in downstream processing and isolation technology, the need for which is largely driven by the stringent re- latory requirements for purity and quality of injectable biopharmaceuticals. This is particularly reflected by the general absence of coverage of this s- ject in many biotechnology and related courses in educational institutions. For a considerable while I have felt that there is increasing need for an introductory text to various aspects of downstream processing, particularly with respect to the needs of the biopharmaceutical and biotechnology ind- try. Although there are numerous texts that cover various aspects of protein purification techniques in isolation, there is a need for a work that covers the broad range of isolation technology in an industrial setting. It is anticipated that Downstream Processing of Proteins: Methods and Protocols will play a small part in filling this gap and thus prove a useful contribution to the field. It is also designed to encourage educational strategists to broaden the coverage of these topics in industrial biotechnology courses by including accounts of this important and rapidly developing element of the industrial process.
Download or read book Protein Purification written by Robert K. Scopes and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 397 pages. Available in PDF, EPUB and Kindle. Book excerpt: New textbooks at all levels of chemistry appear with great regularity. Some fields such as basic biochemistry, organic re action mechanisms, and chemical thermodynamics are weil represented by many excellent texts, and new or revised editions are published sufficiently often to keep up with progress in research. However, some areas of chemistry, especially many of those taught at the graduate level, suffer from a real lack of up-to-date textbooks. The most serious needs occur in fields that are rapidly changing. Textbooks in these subjects usually have to be written by scientists actually involved in the research that is advancing the field. It is not often easy to persuade such individuals to set time aside to help spread the knowledge they have accumulated. Our goal, in this series, is to pinpoint areas of chemistry where recent progress has outpaced what is covered in any available textbooks, and then seek out and persuade experts in these fields to produce relatively concise but instruc tive introductions to their fields. These should serve the needs of one semester or one quarter graduate courses in chemistry and biochemistry. In so me cases the availability of texts in active research areas should help stimulate the creation of new courses.
Download or read book Isolation and Purification of Proteins written by Rajni Hatti-Kaul and published by CRC Press. This book was released on 2003-02-05 with total page 653 pages. Available in PDF, EPUB and Kindle. Book excerpt: This publication details the isolation of proteins from biological materials, techniques for solid-liquid separation, concentration, crystallization, chromatography, scale-up, process monitoring, product formulation, and regulatory and commercial considerations in protein production. The authors discuss the release of protein from a biological host, selectivity in affinity chromatography, precipitation of proteins (both non-specific and specific), extraction for rapid protein isolation, adsorption as an initial step for the capture of proteins, scale-up and commercial production of recombinant proteins, and process monitoring in downstream processing.
Download or read book Protein Liquid Chromatography written by M. Kastner and published by Elsevier. This book was released on 2000 with total page 978 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protein Liquid Chromatography is a handbook-style guide to liquid chromatography as a tool for isolating and purifying proteins, consisting of 25 individual chapters divided into three parts: Part A covers commonly-used, classic modes of chromatography such as ion-exchange, size-exclusion, and reversed-phase; Part B deals with various target protein classes such as membrane proteins, recombinant proteins, and glycoproteins; and Part C looks at various miscellaneous related topics, including coupling reaction, buffer solution additives, and software. The text as a whole can be viewed as a systematic survey of available methods and how best to use them, but also attempts to provide an exhaustive coverage of each facet. How to solve a specific problem using a chosen method is the overall essence of the volume. The principle philosophy of this compilation is that practical application is everything; therefore, both classical and modern methods are presented in detail, with examples involving conventional, medium- and high-pressure techniques. Over-exposure to history, concept, and theory has deliberately been avoided. The reader will find a wealth of tips and tricks from users for users, including advice on the advantages and disadvantages of each method. Easy-to-read sections on "Getting started now" and "Where to go from here" attempt to provide hands-on, fool-proof detailed practical procedures with complete and even standard model runs for any scientist or technician at work in this area.
Download or read book Preparative Chromatography for Separation of Proteins written by Arne Staby and published by John Wiley & Sons. This book was released on 2017-03-06 with total page 577 pages. Available in PDF, EPUB and Kindle. Book excerpt: Preparative Chromatography for Separation of Proteins addresses a wide range of modeling, techniques, strategies, and case studies of industrial separation of proteins and peptides. • Covers broad aspects of preparative chromatography with a unique combination of academic and industrial perspectives • Presents Combines modeling with compliantce useing of Quality-by-Design (QbD) approaches including modeling • Features a variety of chromatographic case studies not readily accessible to the general public • Represents an essential reference resource for academic, industrial, and pharmaceutical researchers
