Download or read book Protein Lipidation in Cell Signaling written by Patrik J. Casey and published by . This book was released on 1995 with total page 4 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Lipid mediated Protein Signaling written by Daniel G.S. Capelluto and published by Springer Science & Business Media. This book was released on 2013-06-17 with total page 227 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides the most updated information of how membrane lipids mediate protein signaling from studies carried out in animal and plant cells. Also, there are some chapters that go beyond and expand these studies of protein-lipid interactions at the structural level. The book begins with a literature review from investigations associated to sphingolipids, followed by studies that describe the role of phosphoinositides in signaling and closing with the function of other key lipids in signaling at the plasma membrane and intracellular organelles.
Download or read book Lipid Second Messengers written by Robert M. Bell and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 326 pages. Available in PDF, EPUB and Kindle. Book excerpt: Lipids traditionally have been viewed as serving two functions: to form cellular membranes and to serve as energy stores. During the last two decades, a new role for lipids has taken center stage: lipids can act as signalling molecules. This book deals with a variety of lipids that have been shown to be messengers. Leading scientists explore all known lipid classes except steroid hormones. Researchers and educators in biochemistry as well as in molecular and cellular biology will appreciate this volume.
Download or read book Protein Lipidation written by and published by Elsevier. This book was released on 2000-10-19 with total page 349 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the first compilation of protein lipidation enzymes. This volume summarizes recent dramatic developments regarding enzymes responsible for protein lipidation, a process critical for a number of physiological functions, including cell proliferation and morphology. Inhibitors of protein lipidation have recently been shown to be useful as anticancer drugs. Enzymatic mechanisms, mutational analysis, and structural studies are presented. - The enzymatic mechanisms of protein lipidation - Three-dimensional structures of protein farnesytransferase, protein geranylgeranytransferase II, and n-myristoryltransferase
Download or read book Biogenesis of Fatty Acids Lipids and Membranes written by Otto Geiger and published by Springer. This book was released on 2019-01-29 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Concise chapters, written by experts in the field, cover a wide spectrum of topics on lipid and membrane formation in microbes (Archaea, Bacteria, eukaryotic microbes).All cells are delimited by a lipid membrane, which provides a crucial boundary in any known form of life. Readers will discover significant chapters on microbial lipid-carrying biomolecules and lipid/membrane-associated structures and processes.
Download or read book Artificial Lipidation as a Novel Molecular Approach to Inhibiting Proteins written by Mirian Avadisian and published by . This book was released on 2015 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Lipidation is a post-translational modification that covalently attaches lipid groups to proteins to restrict their motility to the cell membrane. In this study, we proposed to induce artificial protein-membrane anchorage through the use of a rationally designed Protein-Membrane Anchor (PMA) to inhibit a protein's motility and function within the cell. We hypothesized that induced membrane anchorage of proteins can hold significant therapeutic value when applied to cancer-promoting cell-signaling proteins. Our proof-of-concept PMA 1 was able to sequester and immobilize STAT3, a 93 kDa protein, to the plasma membrane in breast cancer cells. To the best of our knowledge we are the first to design a PMA that targets and restricts the motility of a soluble cytosolic protein. Unfortunately, our second generation PMAs, which incorporated more drug-like, nonphosphorylated molecules, were unable to localize STAT3 to the plasma membrane. However, the PMAs did inhibit STAT3 nuclear translocation in IL6 stimulated breast cancer cells, suggesting that they might be anchoring STAT3 on intracellular membranes. To prevent the physiological drawbacks associated with using large lipidic groups on drugs, we designed prodrug-like PMAs that activate in situ, called PPB-PMAs. The PPB-PMAs incorporate a motif that is recognized by farnesyltransferase to install a farnesyl group, inducing membrane anchorage. Our proof-of-principle PPB was able to undergo farnesylation in an in vitro enzymatic assay as well as anchor in the plasma membrane in breast cancer cells. Currently, the PPB-PMA is being tested for its ability to anchor its target protein, FKBP12. The versatility of the PMA strategy was also demonstrated through the use of metal coordination complex PMAs. These PMAs were able to selectively immobilize phospho-peptides to lipid bilayers. The progress towards designing, synthesizing and testing the novel PMAs is reported herein.
Download or read book New Developments in Lipid Protein Interactions and Receptor Function written by K.W.A. Wirtz and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 319 pages. Available in PDF, EPUB and Kindle. Book excerpt: A NATO Advanced Study Institute on "New Developments in Lipid-Protein Interactions and Receptor Function" was held on the Island of Spetsai, Greece, from August 16-27, 1992. This Institute was organized to bring together researchers in the field of membrane organization and dynamics with those actively involved in studies on receptor function, signal transduction mechanisms and gene regulation. 2 Presentations and discussions focussed on the regulation of intracellular Ca +-levels, on the second messengers derived from inositol lipids and on the specific phospholipase C isozymes involved in these processes. A major focus was on G-proteins and the effect of lipid anchors on their function. These principles of regulation were further discussed in the context of receptors for acetylcholine, lysophosphatidic acid and low-density lipoproteins. In addition, various aspects of the genomic regulation of cell growth and differentiation by transcription factors were presented. These topics were put into perspective by discussing the most recent developments in lipid-protein interactions, protein insertion into membranes, membrane lipid organization and lipid dynamics as mediated by phospholipid transfer proteins. This book presents the content of the major lectures and a selection of the most relevant of the most important topics posters. These proceedings offer a comprehensive account presented during the course of the Institute. The book is intended to make these proceedings accessible to a large audience.
Download or read book Cellular Lipid Binding Proteins written by Jan Jansen and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 229 pages. Available in PDF, EPUB and Kindle. Book excerpt: It is well established that cellular lipid binding proteins serve central roles in cellular lipid uptake and metabolism. Evidence has been presented that various metabolic diseases, such as hyperlipidemia, atherosclerosis, insulin resistance, and diabetes, are characterized by malfunctioning or deficiencies in cellular lipid binding proteins. For better understanding of the action of lipids as signaling compounds and the role of lipids in intermediary metabolism, it is essential to have detailed knowledge of the interactions between lipids and their cognant binding proteins. In view of this growing interest in lipid-protein interaction, the 4th International Conference on Lipid Binding Proteins was held in Maastricht, The Netherlands, in June 2001. The proceedings of the previous three meetings have been published in Molecular and Cellular Biochemistry. The present focused issue of Molecular and Cellular Biochemistry comprises selected papers based on the lectures and posters presented during the 4th conference, and provides insight into the significance of these proteins for the functioning of the cell.
Download or read book Lipid Mediated Signaling written by Eric J. Murphy and published by CRC Press. This book was released on 2010-03-05 with total page 454 pages. Available in PDF, EPUB and Kindle. Book excerpt: As the highly anticipated update to Lipid Second Messengers (CRC Press, 1999), Lipid-Mediating Signaling is a current and comprehensive overview of research methods used in lipid-mediated signal transduction. Pioneering experts provide a much-needed distillation of a decade's worth of advances in research techniques that are pertinent in understand
Download or read book Artificial Lipidation as a Novel Molecular Approach to Inhibiting Proteins written by Mirian Avadisian and published by . This book was released on 2015 with total page 426 pages. Available in PDF, EPUB and Kindle. Book excerpt: Lipidation is a post-translational modification that covalently attaches lipid groups to proteins to restrict their motility to the cell membrane. In this study, we proposed to induce artificial protein-membrane anchorage through the use of a rationally designed Protein-Membrane Anchor (PMA) to inhibit a protein's motility and function within the cell. We hypothesized that induced membrane anchorage of proteins can hold significant therapeutic value when applied to cancer-promoting cell-signaling proteins. Our proof-of-concept PMA 1 was able to sequester and immobilize STAT3, a 93 kDa protein, to the plasma membrane in breast cancer cells. To the best of our knowledge we are the first to design a PMA that targets and restricts the motility of a soluble cytosolic protein. Unfortunately, our second generation PMAs, which incorporated more drug-like, nonphosphorylated molecules, were unable to localize STAT3 to the plasma membrane. However, the PMAs did inhibit STAT3 nuclear translocation in IL6 stimulated breast cancer cells, suggesting that they might be anchoring STAT3 on intracellular membranes. To prevent the physiological drawbacks associated with using large lipidic groups on drugs, we designed prodrug-like PMAs that activate in situ, called PPB-PMAs. The PPB-PMAs incorporate a motif that is recognized by farnesyltransferase to install a farnesyl group, inducing membrane anchorage. Our proof-of-principle PPB was able to undergo farnesylation in an in vitro enzymatic assay as well as anchor in the plasma membrane in breast cancer cells. Currently, the PPB-PMA is being tested for its ability to anchor its target protein, FKBP12. The versatility of the PMA strategy was also demonstrated through the use of metal coordination complex PMAs. These PMAs were able to selectively immobilize phospho-peptides to lipid bilayers. The progress towards designing, synthesizing and testing the novel PMAs is reported herein.
Download or read book Sphingolipid Metabolism and Cell Signaling Part A written by and published by Elsevier. This book was released on 1999-11-01 with total page 786 pages. Available in PDF, EPUB and Kindle. Book excerpt: Sphingolipids are found in all eukaryotic and in some prokaryotic organisms and provide structure for cell membranes, lipoproteins, and other biological materials as well as participate in the regulation of cell growth, differentiation, and diverse cell functions, including cell-cell communication, cell-substratum interactions, and intracellular signal transduction. This volume presents methods used in studying enzymes of sphingolipid biosynthesis and turnover, including inhibitors of some of these enzymes, genetic approaches, and organic and enzymatic syntheses of sphingolipids and analogs. Its companion Volume 312 will contain information on analyzing sphingolipids, sphingolipid transport and trafficking, and sphingolipid-protein interactions and cellular targets.The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
Download or read book Protein Lipid Interactions written by C.Reyes Mateo and published by Springer. This book was released on 2005-12-05 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biological membranes have long been identified as key elements in a wide variety of cellular processes including cell defense communication, photosynthesis, signal transduction, and motility; thus they emerge as primary targets in both basic and applied research. This book brings together in a single volume the most recent views of experts in the area of protein–lipid interactions, providing an overview of the advances that have been achieved in the field in recent years, from very basic aspects to specialized technological applications. Topics include the application of X-ray and neutron diffraction, infrared and fluorescence spectroscopy, and high-resolution NMR to the understanding of the specific interactions between lipids and proteins within biological membranes, their structural relationships, and the implications for the biological functions that they mediate. Also covered in this volume are the insertion of proteins and peptides into the membrane and the concomitant formation of definite lipid domains within the membrane.
Download or read book Lipid Modifications of Proteins written by Patrick J. Casey and published by Academic Press. This book was released on 1995-05-16 with total page 808 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume is organized around the three major classes of lipids that have been identified in covalent attachment to proteins in eukaryotic cells: isoprenoids, saturated fatty acyl groups and glycosylphosphatidylinositol ( GPI ).
Download or read book Lipid Metabolism in Signaling Systems written by John N. Fain and published by . This book was released on 1993 with total page 384 pages. Available in PDF, EPUB and Kindle. Book excerpt: Many of the phospholipases and even the receptor for inositol 1,4,5-trisphosphate have recently been cloned and sequenced and play an important role in neurotransmission. Methods developed in recent years for the study of the enzymes and intermediates involved in the regulation of neuronal signal transduction involving phospholipid turnover are presented in this volume. Major topics covered include: * Techniques for examining the role of phosphoinositides in signal transduction * Techniques related to protein kinase C * Analysis of inositol phosphates * Techniques to analyze phospholipid turnover in the brain
Download or read book Protein lipid Interactions in Plasma Membrane Biogenesis and Cellular Signaling Regulation written by Kirsten Leigh Bryant and published by . This book was released on 2013 with total page 282 pages. Available in PDF, EPUB and Kindle. Book excerpt: It is well established that protein interactions with phospholipids, particularly phosphoinositides, serve to regulate many different cellular processes. Due to the charged nature of biomembranes, electrostatic interactions are particularly effective between proteins and lipids at membrane interfaces where protein oligomerization and locally high charge densities of certain phospholipids can contribute significantly to cellular processes. Phosphoinositide distributions contribute to organelle identity, recruit proteins important for anterograde or retrograde trafficking to the right place at the right time, and directly influence the capacity of proteins to signal to downstream partners. Our studies provide evidence in support of the importance of protein-lipid interactions in the regulation of epidermal growth factor receptor (EGFR) activity and signaling, as well as in biosynthetic trafficking from the endoplasmic recticulum (ER) to the plasma membrane (PM). The polybasic juxtamembrane (JX) region of EGFR has been proposed to regulate protein activity by interacting with the PM and thus preventing tyrosine kinase domain activation. We demonstrate that reduction of the net charge of the JX region in wild-type EGFR results in constitutive activation of the receptor and conferral of its capacity to transform cells in a ligand-independent manner. These capabilities are maintained when the receptor is retained in the endoplasmic reticulum. In addition, we show that the polybasic JX region also plays a positive role in the response of EGFR to ligand. Receptors in which the net positive charge of the JX region is reduced show reduced activation and are deficient in downstream signaling, including their capacity to mediate Ca2+ mobilization. In addition, we demonstrate that protein-lipid interactions also contribute to proper biosynthetic trafficking in cells. We provide pharmacological and molecular genetic evidence for the functional requirement of a pool of phosphoinositide 4-phosphate, synthesized by phosphoinositide 4-kinase III[alpha], for ER to PM protein trafficking. In summary, strategies to perturb both protein structure and phospholipid availability provide evidence in support of roles for basic amino acid sequences and negatively charged phospholipids in biosynthetic trafficking and cellular signaling regulation.
Download or read book Membrane Biogenesis written by Jos A.F. Op den Kamp and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 474 pages. Available in PDF, EPUB and Kindle. Book excerpt: Many individual aspects of the dynamics and assembly of biological membranes have been studied in great detail. Cell biological approaches, advanced genetics, biophysics and biochemistry have greatly contributed to an increase in our knowledge in this field.lt is obvious however, that the three major membrane constituents - lipids, proteins and carbohydrates- are studied, in most cases separately and that a coherent overview of the various aspects of membrane biogenesis is not readily available. The NATO Advanced Study Institute on "New Perspectives in the Dynamics of Assembly of Biomembranes" intended to provide such an overview: it was set up to teach students and specialists the achievements obtained in the various research areas and to try and integrate the numerous aspects of membrane assembly into a coherent framework. The articles in here reflect this. Statting with detailed contributions on phospholipid structure, dynamics, organization and biogenesis, an up to date overview of the basic, lipidic backbone of biomembranes is given. Extensive progress is made in the research on membrane protein biosynthesis. In particular the post- and co-translational modification processes of proteins, the mechanisms of protein translocation and the sorting mechanisms which are necessary to direct proteins to their final, intra - or extracellular destination have been characterized in detail. Modern genetic approaches were indispensable in this research area: gene cloning, hybrid protein construction, site directed mutagenesis and sequencing techniques elucidated many functional aspects of specific nucleic acid and amino acid sequences.
Download or read book Protein Lipidation Protocols written by Michael H. Gelb and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 243 pages. Available in PDF, EPUB and Kindle. Book excerpt: In Protein Lipidation Protocols, Michael Gelb brings together a collection of readily reproducible techniques for studying protein lipidation, the covalent attachment of lipids to proteins. These cutting-edge methods-many never published before in a "hands-on" format-deal with glycosyl phosphatidylinositol (GPI)-containing compounds, protein fatty acylation, and protein prenylation. Included are novel techniques for determining the chemical structure of GPI-anchors, for radiolabeling the prenyl groups of protein in eukaryotic cells, a tool for developing inhibitors of the protein farnesyltransferase, and for an exciting lysosomal enzyme that cleaves fatty acyl groups from proteins, the first fatty acylase discovered. Protein Lipidation Protocols offers biochemists, cell and molecular biologists, medicinal chemists, and pharmaceutical researchers state-of-the-art tools for understanding the complex biochemistry of protein lipidation, as well as catalyzing the development of many important new biopharmaceuticals, including anticancer drugs.
