Download or read book Capillary Electrophoresis of Proteins and Peptides written by Mark A. Strege and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 340 pages. Available in PDF, EPUB and Kindle. Book excerpt: Throughout the more than 20 years that have followed the beginnings of capillary electrophoresis (CE), its application to the analysis of proteins and peptides has continued to be reliable, versatile, and productive. Over time, CE has matured to become a superb complement to HLPC, and in many cases has also evolved as an automated and quantitative replacement for conventional slab gel electrophoresis methods such as SDS-PAGE and isoelectric focusing. Within Capillary Electrophoresis of Proteins and Peptides, we have assembled contributions from researchers who are applying state-of-the-art CE for protein and peptide analysis, including topics that we believe are of great potential both in the present and for the future. In comparison to traditional separation methods, CE represents a miniaturized analysis technique (especially in its microchip-based format) that is highly dependent upon the basic fundamentals of effective sample recovery and high sensitivity detection. With these issues in mind, Chapters 1–4 describe recently developed approaches for both capillary coatings and analyte detection via laser-induced fluorescence. Since the discipline of biotechnology has established itself as a primary platform for the application of CE to the analysis of proteins and peptides, Chapters 5–7 demonstrate a variety of examples of the specific techniques that have been applied for the development of biopharmaceuticals and their commercialization. The methods covered here include also the analysis of oligosaccharides from glycoproteins.

Download or read book Capillary Gel Electrophoresis written by Andras Guttman and published by Newnes. This book was released on 2021-12-04 with total page 391 pages. Available in PDF, EPUB and Kindle. Book excerpt: Capillary Gel Electrophoresis and Related Microseparation Techniques covers all theoretical and practical aspects of capillary gel electrophoresis. It also provides an excellent overview of the key application areas of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods. It not only gives readers a better understanding of how to utilize this technology, but also provides insights into how to determine which method will provide the best technical solutions to particular problems. This book can also serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses. - Covers all theoretical and practical aspects of capillary gel electrophoresis - Excellent overview of the key applications of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods - Teaches readers how to use the technology and select methods that are ideal for fundamental problems - Can serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses

Download or read book Handbook of Capillary Electrophoresis Second Edition written by James P. Landers and published by CRC Press. This book was released on 1996-12-23 with total page 918 pages. Available in PDF, EPUB and Kindle. Book excerpt: Because new information was discovered at an incredible rate since the publication of the successful first edition of this Handbook, this fully updated second edition covers all areas of interest in the field of capillary electrophoresis (CE). A relatively new technology, CE is a principle method for studying the physicochemical properties of proteins, peptides, and other macromolecules. Where applicable, the 30 chapters provide basic underlying theories as well as application-oriented aspects of each technique.Keep up with all the developments in this growing field with the Handbook of Capillary Electrophoresis, Second Edition - a complete guide to the fundamentals of CE and the latest research. The chapters are organized into five units: Modes: Presents a theoretical development of the basic principles governing separation with several modes, including CEC, and discusses their practical aspects. Analyte: Applies CE to the analysis of a specific class of analytes, including organic and inorganic ions, pharmaceuticals, glycoconjugates, peptides, proteins, and DNA fragments. Fundamental Aspects of CE: Technique-oriented information for the practitioner, including the importance of the sample matrix, on-line preconcentration of samples, modes of detection, and specific aspects of CE data analysis. Applications of CE: Includes single cell analysis, CE in DNA sequencing, CE as a clinical diagnostic tool, identifying and quantifying drugs, and for characterizing interacting species. Specialized Aspects of CE: Discusses interfacing CE with mass spectrometry, high-volume throughput continuous CE, microchip CE, control of EOF, and much more. The Handbook of Capillary Electrophoresis, Second Edition, pulls together diverse areas and applications of CE, resulting in an excellent tool for scientists involved in biotechnology and clinical chemistry, as well as the pharmaceutical, bioscience, chemical, and instrument-manufacturing industries. With an applications-oriented focus, the handbook is also a superb manual for workshops, seminars, and graduate courses in separation science.

Download or read book Handbook of Capillary and Microchip Electrophoresis and Associated Microtechniques written by James P. Landers and published by CRC Press. This book was released on 2007-12-18 with total page 1598 pages. Available in PDF, EPUB and Kindle. Book excerpt: Now in its third edition, this bestselling work continues to offer state-of-the-art information on the development and employment of capillary electrophoresis. With special emphasis on microseparations and microfluidics, it features new chapters describing the use of microchip electrophoresis and associated microtechniques, with a focus on the extraordinary breadth of work undertaken to expand CE methodologies in recent years. Enhanced by contributions from leading international experts, the Handbook of Capillary and Microchip Electrophoresis and Associated Microtechniques, Third Edition remains a seminal reference for the chemistry, biology, and engineering fields.

Download or read book Analytical and Preparative Separation Methods of Biomacromolecules written by Hassan Y. Aboul-Enein and published by CRC Press. This book was released on 2020-10-15 with total page 482 pages. Available in PDF, EPUB and Kindle. Book excerpt: Reports up-to-date research developments on purifying and isolation large organic molecules. The text provides information on high-performance liquid chromatography and capillary electrophoresis (CE) as tools for analyzing biomacromolecules and developing new biochemical and medicinal compounds. It applies biochemical separation technology to the study of macromolecules such as proteins, polysaccharides, nucleic acids and more.

Download or read book Advances in Protein Chemistry and Structural Biology written by Alexander McPherson and published by Academic Press. This book was released on 2010-09-09 with total page 241 pages. Available in PDF, EPUB and Kindle. Book excerpt: Structural genomics is the systematic determination of 3-D structures of proteins representative of the range of protein structure and function found in nature. The goal is to build a body of structural information that will predict the structure and potential function for almost any protein from knowledge of its coding sequence. This is essential information for understanding the functioning of the human proteome, the ensemble of tens of thousands of proteins specified by the human genome. While most structural biologists pursue structures of individual proteins or protein groups, specialists in structural genomics pursue structures of proteins on a genome wide scale. This implies large-scale cloning, expression and purification. One main advantage of this approach is economy of scale. - Examines the three dimensional structure of all proteins of a given organism, by experimental methods such as X-ray crystallography and NMR spectroscopy - Looks at structural genomics as a foundation of drug discovery as discovering new medicines is becoming more challenging and the pharmaceutical industry is looking to new technologies to help in this mission

Download or read book Physical Biology written by Ahmed H. Zewail and published by Imperial College Press. This book was released on 2008 with total page 582 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is an avant-garde book edited by Nobel Laureate Ahmed Zewail with contributions from eminent scientists including four Nobel prize winners. The perspectives of these world leaders in physics, chemistry, and biology define potential new frontiers at the interface of disciplines and including physical, systems, and synthetic biology.This book brings about the confluence of concepts and tools, and that of different disciplines, to address significant problems of our time: visualization; theory and computation for complexity; macromolecular function, protein folding and misfolding; and systems integration from cells to consciousness. The scope of tools is wide-ranging, spanning imaging, crystallography, microfluidics, single-molecule spectroscopy, and synthetic probe targeting. Concepts such as dynamic self-assembly, molecular recognition, non-canonical amino acids, and others are covered in various chapters as they are cornerstones in building the trilogy description of behavior-structure, dynamics, and function.The volume is uniquely structured to provide overviews with historical perspectives on the evolution of ideas and on the future of physical biology and biological complexity, from atoms to medicine.

Download or read book Biophysico Chemical Processes Involving Natural Nonliving Organic Matter in Environmental Systems written by Nicola Senesi and published by John Wiley & Sons. This book was released on 2009-07-23 with total page 905 pages. Available in PDF, EPUB and Kindle. Book excerpt: An up-to-date resource on natural nonliving organic matter Bringing together world-renowned researchers to explore natural nonliving organic matter (NOM) and its chemical, biological, and ecological importance, Biophysico-Chemical Processes Involving Natural Nonliving Organic Matter in Environmental Systems offers an integrated view of the dynamics and processes of NOM. This multidisciplinary approach allows for a comprehensive treatment encompassing all the formation processes, properties, reactions, environments, and analytical techniques associated with the latest research on NOM. After briefly outlining the historical background, current ideas, and future prospects of the study of NOM, the coverage examines: The formation mechanisms of humic substances Organo-clay complexes The effects of organic matter amendment Black carbon in the environment Carbon sequestration and dynamics in soil Biological activities of humic substances Dissolved organic matter Humic substances in the rhizosphere Marine organic matter Organic matter in atmospheric particles In addition to the above topics, the coverage includes such relevant analytical techniques as separation technology; analytical pyrolysis and soft-ionization mass spectrometry; nuclear magnetic resonance; EPR, FTIR, Raman, UV-visible adsorption, fluorescence, and X-ray spectroscopies; and thermal analysis. Hundreds of illustrations and photographs further illuminate the various chapters. An essential resource for both students and professionals in environmental science, environmental engineering, water science, soil science, geology, and environmental chemistry, Biophysico-Chemical Processes Involving Natural Nonliving Organic Matter in Environmental Systems provides a unique combination of the latest discoveries, developments, and future prospects in this field.

Download or read book A Mathematical Approach to Protein Biophysics written by L. Ridgway Scott and published by Springer. This book was released on 2017-12-04 with total page 295 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book explores quantitative aspects of protein biophysics and attempts to delineate certain rules of molecular behavior that make atomic scale objects behave in a digital way. This book will help readers to understand how certain biological systems involving proteins function as digital information systems despite the fact that underlying processes are analog in nature. The in-depth explanation of proteins from a quantitative point of view and the variety of level of exercises (including physical experiments) at the end of each chapter will appeal to graduate and senior undergraduate students in mathematics, computer science, mechanical engineering, and physics, wanting to learn about the biophysics of proteins. L. Ridgway Scott has been Professor of Computer Science and of Mathematics at the University of Chicago since 1998, and the Louis Block Professor since 2001. He obtained a B.S. degree (Magna Cum Laude) from Tulane University in 1969 and a PhD degree in Mathematics from the Massachusetts Institute of Technology in 1973. Professor Scott has published over 130 papers and three books, extending over biophysics, parallel computing and fundamental computing aspects of structural mechanics, fluid dynamics, nuclear engineering, and computational chemistry. Ariel Fernández (born Ariel Fernández Stigliano) is an Argentinian-American physical chemist and mathematician. He obtained his Ph. D. degree in Chemical Physics from Yale University and held the Karl F. Hasselmann Endowed Chair Professorship in Bioengineering at Rice University. He is currently involved in research and entrepreneurial activities at various consultancy firms. Ariel Fernández authored three books on translational medicine and biophysics, and published 360 papers in professional journals. He holds two patents in the field of biotechnology.

Download or read book Index Medicus written by and published by . This book was released on 2004 with total page 2036 pages. Available in PDF, EPUB and Kindle. Book excerpt: Vols. for 1963- include as pt. 2 of the Jan. issue: Medical subject headings.

Download or read book Quantitive Analysis Of Biospec written by Per Lundahl and published by Routledge. This book was released on 2021-09-01 with total page 244 pages. Available in PDF, EPUB and Kindle. Book excerpt: First published in 2004, this book collects several up-to-date methods for quantitative analysis of biospecific interactions, a field that has a long history that perhaps can be said to have begun with the classical paper of G. Scatchard in 1949 (The attractions of proteins for small molecules and ions, but which has advanced impressively during the last few years. A precise spatial arrangement of just a few hydrogen bonds can confer a remarkably specific reversible association between two molecules. A web of weak interactions governs biospecific recognition in general. The binding equilibria in living cells tune and coordinate a multitude of functions. The thermodynamic properties of such interactions are often studied by binding experiments in simplified and essentially ideal systems. However, similar types of studies may elucidate the biologically relevant dynamic steady-state conditions in living cells and organisms, allowing for the very wide range of interactant concentrations and the interplay between the many reactions and interactions. The development in biosciences will continue with in-depth studies of macromolecules and membranes. More detailed knowledge will allow analyses of delicate balances between substances and events in the complex systems involved in life processes. Methods to study biospecific affinities are thus highly important tools for understanding mechanisms and effects of molecular binding events in vivo and in vitro, e.g., in biochemical, biomedical and pharmaceutical research, and for biotechnological research and production.

Download or read book Quantitative Chemical Analysis Sixth Edition written by Daniel C. Harris and published by Macmillan. This book was released on 2003 with total page 948 pages. Available in PDF, EPUB and Kindle. Book excerpt: For instructors who wish to focus on practical, industrial, or research chemistry. Includes case studies, applications boxes, and spreadsheet applications.

Download or read book Encyclopedia of Analytical Science written by and published by Elsevier. This book was released on 2019-04-02 with total page 5142 pages. Available in PDF, EPUB and Kindle. Book excerpt: The third edition of the Encyclopedia of Analytical Science, Ten Volume Set is a definitive collection of articles covering the latest technologies in application areas such as medicine, environmental science, food science and geology. Meticulously organized, clearly written and fully interdisciplinary, the Encyclopedia of Analytical Science, Ten Volume Set provides foundational knowledge across the scope of modern analytical chemistry, linking fundamental topics with the latest methodologies. Articles will cover three broad areas: analytical techniques (e.g., mass spectrometry, liquid chromatography, atomic spectrometry); areas of application (e.g., forensic, environmental and clinical); and analytes (e.g., arsenic, nucleic acids and polycyclic aromatic hydrocarbons), providing a one-stop resource for analytical scientists. Offers readers a one-stop resource with access to information across the entire scope of modern analytical science Presents articles split into three broad areas: analytical techniques, areas of application and and analytes, creating an ideal resource for students, researchers and professionals Provides concise and accessible information that is ideal for non-specialists and readers from undergraduate levels and higher

Download or read book Quantitative Chemical Analysis written by Daniel C. Harris and published by Macmillan. This book was released on 2010-04-30 with total page 892 pages. Available in PDF, EPUB and Kindle. Book excerpt: QCA is the bestselling textbook of choice for analytical chemistry. It offers a modern portrait of the techniques of chemical analysis, backed by a wealth of real world applications. This edition features new coverage of spectroscopy and statistics, new pedagogy and enhanced lecturer support.

Download or read book Annual Reports in Organic Synthesis 1999 written by Philip M. Weintraub and published by Gulf Professional Publishing. This book was released on 1999-08-23 with total page 428 pages. Available in PDF, EPUB and Kindle. Book excerpt: One of the more difficult problems facing chemists today is that of "keeping up with the literature." Thus, an organized annual review of synthetically useful information would prove beneficial to nearly all organic chemists, both specialists and non-specialists in synthesis. Weintraub et al. are very successful in creating such an original review. Key Features * Provides clearly illustrated structures of compounds in every feasible synthetic pathway * Presents a general review of structure/activity information for each synthetic compound * Includes extensive reference information on additional publications available for each reaction discussed * Yields a current awareness of the literature, ideas for research, and questions for cumulative exams * Highlights papers dealing with asymmetric syntheses * Remains the most comprehensive, reasonably priced compilation of its type

Download or read book Chemical Reagents for Protein Modification written by Roger L. Lundblad and published by CRC Press. This book was released on 2014-07-22 with total page 678 pages. Available in PDF, EPUB and Kindle. Book excerpt: The use of the chemical modification of proteins has evolved over the past 80 years, benefiting from advances in analytical, physical, and organic chemistry. Over the past 30 years, the use of chemical reagents to modify proteins has been crucial in determining the function and structure of purified proteins. This groundbreaking work is part of the

Download or read book Peptide and Protein Drug Analysis written by Ronald Reid and published by CRC Press. This book was released on 1999-11-12 with total page 926 pages. Available in PDF, EPUB and Kindle. Book excerpt: Furthering efforts to simulate the potency and specificity exhibited by peptides and proteins in healthy cells, this remarkable reference supplies pharmaceutical scientists with a wealth of techniques for tapping the enormous therapeutic potential of these molecules-providing a solid basis of knowledge for new drug design. Provides a broad, comprehensive overview of peptides and proteins as mediators of cell movement, proliferation, differentiation, and communication. Written by more than 50 leading international authorities, Peptides and Protein Drug Analysis discusses strategies for dealing with the complexity of peptides and proteins in conformational flexibility and amino acid sequence variability analyzes drug formulations facilitated by solid-phase peptide synthesis and recombinant DNA technology examines chemical purity analysis by high-pressure chromatographic, capillary electrophoretic, gel electrophoretic, and isoelectric focusing methods highlights drug design elements derived from protein folding, bioinformatics, and computational chemistry demonstrates uses of unnatural mutagenesis and combinatorial chemistry explores mass spectrometry, protein sequence, and carbohydrate analysis illustrates bioassays and other new functional analysis methods surveys spectroscopic techniques such as ultraviolet, fluorescence, Fourier transform infrared, and nuclear magnetic resonance (NMR) addresses ways of distinguishing between levels of therapeutic and endogenous agents in cells reviews structural analysis tools such as ultracentrifugation and light, X-ray, and neutron scattering and more! Featuring over 3400 bibliographic citations and more than 500 tables, equations, and illustrations, Peptide and Protein Drug Analysis is a must-read resource for pharmacists; pharmacologists; analytical, organic, and pharmaceutical chemists; cell and molecular biologists; biochemists; and upper-level undergraduate and graduate students in these disciplines.