Download or read book Production of Cellulase Enzyme from Trichoderma Reesei QM9414 Using Ground Oil Palm Trunk as the Substrate in a Packed Reactor written by Putri Faridatul Akmar and published by . This book was released on 1991 with total page 7 pages. Available in PDF, EPUB and Kindle. Book excerpt: The possibility of using ground oil palm trunk as substrate for production of cellulase in a packed reactor was investigated. The maximum activity of B-glucosidase (323.6x10 -3 IU/ml) was detected after 360hr after inoculation and carboxymethycellulase activity (119.0x10 -3 IU/ml) after 228hr after inoculation. [Authors' abstract].
Download or read book Utilisation of Oil Palm Trunk as Substrate for the Production of Cellulase from Trichoderma Reesei QM9414 in a Packed Bioreactors written by Putri Faridatul Akmar and published by . This book was released on 1993 with total page 3 pages. Available in PDF, EPUB and Kindle. Book excerpt: Comparison with respect to enzymes production between the two substrate can be seen from Fig. 1.0. Enzymes produced when oil palm trunk was used as substrate were higher in activity compared to when cellulose floc was used as substrate. The maximum production of Filter Paper Activity and carboxymethyl cellulase activity was observed on the 4th day with 0.40 and 0.66 Uml-1 respectively whilst B-glucosidase activity was detected much later on the 16th day at 0.05 Uml-1. The results obtained indicated that oil palm trunk was found to be suitable as substrate for maintaining the growth of Trichoderma reesei QM9414 as wel as for the production f cellulolytic enzymes. [Authors' abstract].
Download or read book Cellulase Production by Trichoderma Reesei Rut C30 written by Chi-Ming Lo and published by . This book was released on 2008 with total page 223 pages. Available in PDF, EPUB and Kindle. Book excerpt: As the price of oil continues to increase and the concern over global warming heightens, finding alternative renewable sources of energy becomes more and more imperative. Considering the abundance of lignocellulosic biomass, the potential significance of its conversion to fuel such as ethanol has long been recognized. Ethanol production from lignocellulosic biomass requires the breakdown of the solid material to simple sugars that can be consumed by microorganisms. The breakdown of lignocelluloses includes the important step of hydrolyzing cellulose, which are [Beta]-1, 4 linked polymers of glucose, by a group of enzymes collectively termed cellulase. The cost of cellulase production profoundly influences the economics of the entire ethanol production process. Trichoderma reesei Rut C-30 is the most commonly used fungal strain for industrial cellulase production. With an overall goal of decreasing the cellulase production cost, this thesis work was focused on two topics: (1) investigation of the performance of different cellulase-inducing substrates in T. reesei Rut C-30 fermentation and (2) development of a more advanced model to describe the culture behaviors of T. reesei Rut C-30 grown on lactose-based media. The cellulase-inducing substrates investigated in this work included the acid-treated hydrolysate, lactose, and the sophorolipids produced by the yeast Candida bombicola. In addition to the above focused topics, the effects of culture conditions on cellulase production were also investigated. The acid-treated hardwood hydrolysates used in the study were prepared by a two-stage process, with different durations of boiling and acid concentrations. The results indicated that the inducing ability of the hydrolysates decreased with increasing boiling time (less oligomers). The observation was attributed to the lower amounts of inducing oligomers remaining after the longer boiling in acid. When compared with cultures growing on mixed carbon substrates of cellulose and glucose, the culture growing on hydrolysates showed a longer lag phase of about 24 hours (before the active cell growth began), but produced adequately comparable final cellulase activity. The study with lactose as an inducing substrate was conducted in both batch and continuous culture systems with lactose and other relevant substrates (glycerol, glucose and galactose) as the carbon source. Instead of direct ingestion, lactose is believed to be hydrolyzed by extracellular enzymes to glucose and galactose, which are then taken up by the cells. The study results indicated that glucose strongly represses the galactose metabolism: Cells started to consume galactose only after the glucose had been depleted. A mathematical model incorporating all important metabolic activities was developed to describe the culture behaviors. All of the experimental results obtained were used in model fitting to generate a set of best-fit model parameters. The study provided significant conceptual and quantitative insights to the lactose metabolism and cellulase production by T. reesei Rut C-30. This study was also the first to hypothesize and demonstrate the use of sophorolipids as the inducing substrate for cellulase production. A unique process for cellulase production using a mixed culture of T. reesei Rut C30 and Candida Bombicola growing on glycerol-based media was investigated. Hypothetically, the sophorolipids produced by C. bombicola were hydrolyzed to form sophorose, which then served as the inducer for cellulase production by T. reesei. Further study to optimize the sophorolipid-induced cellulase production process is recommended.
Download or read book Pilot Plant Production of Cellulase by Trichoderma Reesei QM9414 and the Effect of Dimethyl Sulfoxide on Cellulase Production by T Reesei MCG77 written by Paul J. Whalen and published by . This book was released on 1983 with total page 210 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Cellulase Enzyme Using Pome Production and Application written by Shah Samiur Rashid and published by LAP Lambert Academic Publishing. This book was released on 2012-05 with total page 264 pages. Available in PDF, EPUB and Kindle. Book excerpt: The study was conducted for the production of cellulase enzyme through liquid state bioconversion of palm oil mill effluent (POME) by filamentous fungi and characterization of the produced enzyme. Several experiments such as selection of filamentous fungi, optimization of media and process parameters, development of the separation/purification conditions for ultra-filtration, characterization and evaluation of purified cellulase enzyme through hydrolysis of oil palm empty fruit bunches (EFB) were carried out to achieve the entire objectives of the study. The results on isolation indicated that among the strains isolated, the strains IBPC108R, IBEC302B and IBEC305B gave the highest FPAse activity (0.3 U/ml) after 2 days of fermentation and IBEC302B showed the highest CMCase activity (0.42 U/ml) after 3 days of fermentation. Among the laboratory stocks, Aspergillus IBO-102MNB, gave the highest FPAse activity (0.28 U/ml) and Aspergillus IBO-103MNB gave the highest CMCase activity (0.62 U/ml) after 2 days of fermentation. Among the strains tested, the Trichoderma reesei RUT C-30, a commercial
Download or read book Cellulase Enzyme Production from Trichoderma Reesei written by Solomon Sudhakar Magapu and published by . This book was released on 2013-08-30 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Treatment of Oil Palm Empty Fruit Bunch Fiber for Subsequent Use as Substrate for Cellulase Production by Aspergillus Terreus Suk 1 written by Norhayati Abu Bakar and published by . This book was released on 2006 with total page 56 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cellulases are important in industrial enzyme which is a main part in industrial process such as in bio-bleaching and bioethanol production. Nowadays, our country encourages the production of bioethanol from the biomass material such as oil palm empty fruit bunch fiber in order to enhance the resources of ethanol production and reduce the cost.In this project, oil palm empty fruit bunch fiber was used as a substrate for the production of cellulase enzyme by Aspergillus terreus SUK-1. In order to enhance the cellulase production, pretreatment on substrate are performed with different type of methods. Comparison results of cellulase enzyme production are determined between the substrate which is pretreated with acid and alkaline pretreatment. The effect of steam explosion on the substrate also was identified in shaking flask fermentation. From the experimental work, it was found that the highest xylanase and carboxymethylcellulase activity was in fermentation of acid treated substrate than alkali treated substrate and untreated substrate It also found that by autoclaved the substrate with chemicals produced the highest cellulase enzyme with value 0.167836U/ml for xylanase and 0.3534U/ml for carboxymethylcellulase.
Download or read book Collection of Trichoderma Reesei Cellulase by Foaming written by Qin Zhang and published by . This book was released on 2007 with total page 197 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Facing the current energy crisis, people try to find alternative energy sources. Certain microbes can ferment soluble sugars to ethanol, which is a well known biofuel. Cellulosic biomass is the most abundant renewable resource. Cellulose can be broken down to soluble sugars by the cellulase enzymes produced only by some microbes. The combination of the above two processes, i.e., break-down of cellulose to sugars and conversion of sugars to ethanol, represents a major approach of solving the energy crisis through renewable biological processes. One of the bottle necks of this approach is the relatively high production cost of cellulase. In this research, the main task was to develop a separation process for cellulase using cost-effective and environment-friendly foam-based techniques. First, the foaming properties of the fermentation broth of Trichoderma reesei RUT C-30 were investigated. The experiment results showed that cellulase was not the dominant substance that caused the foaming of the fermentation broth, although the increase of cellulase concentration in the fermentation process appeared to parallel the increase in foaming tendency. Cellulase concentration was found to be only a weakly positive factor contributing to the foaming whereas the presence of cells and solid cellulose powders had negative effects on foaming. The cell wall was found to be hydrophobic according to the study conducted to observe the cells partition between hydrophobic (organic solvent: n-hexadecane) phase and hydrophilic (aqueous) phase. The finding of hydrophobic cell wall was consistent with the observation that the cells would come out with foam in the foaming study. To prevent the cell from being foamed out of the fermentor during the foaming process intended for cellulase collection, the cell immobilization in small (3mm*3mm*3mm) pieces of polyurethane (PU) foams with different porosity and pore size was also investigated. With some of the PU foam materials, the cells could be effectively immobilized and would not be foamed out during the foaming process. Nevertheless, this system did not function well in the typical stir-tank fermentor: the high shear stress present near the tip of the impeller blades appeared to strip the immobilized cells off the PU foam pellets and caused significant subsequent growth of free (not immobilized) cells in the broth. In a subsequent study of cellulase production using cellulose hydrolysate (CH) as the inducing substrate, it was observed that the addition of the hydrolysate resulted in higher enrichment ratios of cellulase concentration in the collected foamate (over the residual concentration in the spent broth). The foaming study was then expanded to include the addition of other cellulase substrates and substrate analogs, such as xylan hydrolysate (XH) and carboxylmethylcellulose (CMC). The results clearly demonstrated the addition of CH, XH and CMC would improve the enrichment and purification of cellulases by foaming. The improvements were attributed to the affinity binding between the substrates/substrate analogs and the cellulase enzymes and the increased partition of the substrate-enzyme complexes to the foam's air-liquid interfaces. To further pursue the above affinity foaming phenomenon for significantly improved collection and, potentially, purification of cellulase, a polymeric foaming agent was designed and synthesized. The polymeric surfactant has two parts, one is the hydrophobic region containing methylmethacrylate (MMA) and methacrylic acid (MAA), the other part is cellobiose, i.e., the substrate of the enzyme to bind with during foaming process. Unfortunately, the foaming ability of this synthetic surfactant was not good enough and its synthesis was complicated. As an easier first step, two glycolipid biosurfactants, sophorolipids and rhamnolipids, were investigated as the possible affinity foaming agents. Both glycolipids had a disaccharide moiety that was expected to bind selectively to the â-glucosidase in cellulase. While sophorolipids did not foam well, rhamnolipids showed great foaming ability and selectivity towards â-glucosidase. The enrichment ratio of â-glucosidase activity in the foamate reached up to 20 in the solutions prepared by cellulase powders (from Sigma). The significant benefits of such affinity foam collection and/or fractionation techniques were clearly shown."--Abstract.
Download or read book Experimental and Theoretical Studies on the Production of Cellulase by Trichoderma Reesei QM9414 Incorporating Particle Size and Filtration Effects written by Audrey McNulty and published by . This book was released on 1998 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Study of the Production of Cellulases with Trichoderma Reesei and Investigation of the Enzymatic Hydrolysis of Cellulosic Substrates written by Roberto Leyva and published by . This book was released on 1984 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Production of Trichoderma Reesei Cellulase and Enzymatic Hydrolysis of Lignocellululosic Materials for Chemical Feedstock Production written by and published by . This book was released on 1984 with total page 10 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Optimisation of Cellulase Production by Trichoderma Reesei Qm 9414 written by Wan Mohtar Wan Yusoff and published by . This book was released on 1986 with total page 4 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Fungal Metabolites written by Jean-Michel Mérillon and published by . This book was released on with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Feasibility Study of an Owner operator System for Producing Trichoderma Reesei QM 9414 Cellulase written by Carla M. Cass and published by . This book was released on 1984 with total page 112 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Enhanced Production of Cellulase Hemicellulase and glucosidase by Trichoderma Reesei Rut C 30 written by and published by . This book was released on 1981 with total page 13 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this paper, the Production of cellulases and Hemicellulases was studied with Trichoderma reesei Rut C-30, This organism produced, together with high cellulase activities, considerable amounts of xylanases and ?-glucosidase. Three cellulose concentration (1, 2.5, and 5.0%) were examined to determined the maximum levels of cellulase activity obtainable in submerged culture. Temperature and pH profiling was used to increase cell mass to maximum levels within two days and thereby enhancing fermentor productivity at higher substrate levels. Finally, the effect of temperature, pH, Tween-80 concentration, carbon sources, and substrate concentration on the ration of mycelial growth and extracellulose enzyme production are described.
Download or read book The Relative Activity of the Cellulase Enzyme System of Trichoderma Reesei with Native and Modified Cellulosic Substrates written by Ean-Tun Liaw and published by . This book was released on 1994 with total page 302 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Biosynthesis of Cellulase system from Trichoderma Reseei i e Reesei Characteristics written by Victor Ankang Awafo and published by . This book was released on 1997 with total page 418 pages. Available in PDF, EPUB and Kindle. Book excerpt:
