Download or read book Probing Weak Single molecule Interactions written by Kenneth Anders Halvorsen and published by . This book was released on 2007 with total page 296 pages. Available in PDF, EPUB and Kindle. Book excerpt: Abstract: The focus of this thesis is the development, verification, and scientific use of a force probe designed to explore weak interactions between or within single biomolecules. The developed system utilizes a laser optical trap to apply piconewton forces to a functionalized probe bead that interacts with a reactive test bead, and high-speed video processing to measure nanometer displacements of both beads at a rate of ~1500 Hz. The position of the probe bead is used to report both the force on a single molecular bond and the change in length due to protein unfolding or refolding. Several feedback and automation systems have been integrated to facilitate the repetitive testing required to characterize these stochastic interactions. The measurement and application of piconewton-sized forces depends on accurate position detection and proper calibration of the optical trap. The accuracy of the position detection was determined to be 2-3 nm by analyzing a non-moving bead. To assess optical trap calibration, a detailed study and comparison of common methods was performed. Noise-based methods which rely on the variance or power spectrum were found to have systematic errors due to the finite integration time of the detection. To solve this problem, the effect of integration time on these measurements was quantified and new calibration methods were developed. Finally, the instrument was used to study the forced unfolding of the spectrin repeat, a small modular protein domain found in various load supporting proteins. The unfolding kinetics were determined by pulling on the molecule over several orders of magnitude in loading rate. The findings indicate that spectrin unfolding is governed by a single prominent energy barrier, characterized by a force scale of 2.5 pN and a stress-free unfolding rate of 0.003 s -1.

Download or read book Probing Weak Copper Chaperone Wilson Disease Protein Interactions at the Single molecule Level with Nanovesicle Trapping written by Jaime Benitez and published by . This book was released on 2010 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Copper is an essential cofactor for many metalloproteins, yet it can also be cytotoxic, therefore, intracellular copper trafficking is tightly regulated. Copper delivery inside cells is mediated by copper chaperones, which bind and deliver copper to their target proteins, preventing adventitious chemical reactions with the metal. Various pathways for copper transport exist; of particular interest to this thesis is the pathway between the copper chaperone Hah1 and Wilson disease protein (WDP). Limited dynamic information is available on how the copper chaperone Hah1 and the metal binding domains (MBDs) of WDP interact for copper transfer. In this thesis, the interaction dynamics of Hah1 and a single MBD of WDP is investigated. Since these protein-protein interactions are relatively weak in nature, a nanovesicle trapping strategy is used to increase the effective concentration of single molecules (Chapter 2). Individual interaction events are then monitored by single-molecule Forster resonance energy transfer (smFRET). The interaction dynamics of Hah1 and the fourth MBD (MBD4) of WDP are initially studied in the absence of copper. The protein-protein interaction scheme and associated rate constants for the interaction process are extracted from the FRET efficiency (EFRET) and waiting-time distributions (Chapter 3). The EFRET distributions obtained from interactions in the absence and presence of copper are then used to gain insight on the underlying copper transfer process (Chapter 4).

Download or read book Biology at the Single Molecule Level written by S.H. Leuba and published by Elsevier. This book was released on 2001-12-17 with total page 263 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the first book solely devoted to single-molecule biochemistry and molecular biology. Authors were selected on the basis of their contribution to this new and exciting field, and were asked to focus more on the biological problems that can be approached using single-molecule techniques rather than on the techniques per se. It is thought that such techniques will eventually dominate the physical characterization of biologically important macromolecules.

Download or read book Recent Advances in Fluorescent Probes for Super Resolution Microscopy written by Chayan Kanti Nandi and published by Frontiers Media SA. This book was released on 2021-07-21 with total page 105 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Roadmap of Scanning Probe Microscopy written by Seizo Morita and published by Springer Science & Business Media. This book was released on 2006-12-30 with total page 207 pages. Available in PDF, EPUB and Kindle. Book excerpt: Scanning tunneling microscopy has achieved remarkable progress and become the key technology for surface science. This book predicts the future development for all of scanning probe microscopy (SPM). Such forecasts may help to determine the course ultimately taken and may accelerate research and development on nanotechnology and nanoscience, as well as all in SPM-related fields in the future.

Download or read book Nanoscale Probes of the Solid Liquid Interface written by Andrew A. Gewirth and published by Springer Science & Business Media. This book was released on 2013-04-17 with total page 340 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nanoscale Probes of the Solid--Liquid Interface deals with the use of the scanning tunnelling microscope (STM) and related instrumentation to examine the phenomena occurring at the interface between solid and liquid. Scanning probe microscopy (the collective term for such instruments as the STM, the atomic force microscope and related instrumentation) allows detailed, real space atomic or lattice scale insight into surface structures, information which is ideally correlated with surface reactivity. The use of SPM methods is not restricted to ultrahigh vacuum: the STM and AFM have been used on samples immersed in solution or in ambient air, thus permitting a study of environmental effects on surfaces. At the solid--liquid interface the reactivity derives precisely from the presence of the solution and, in many cases, the application of an external potential. Topics covered in the present volume include: the advantages of studying the solid--liquid interface and the obtaining of additional information from probe measurements; interrelationships between probe tip, the interface and the tunnelling process; STM measurements on semiconductor surfaces; the scanning electrochemical microscope, AFM and the solid--liquid interface; surface X-ray scattering; cluster formation on graphite electrodes; Cu deposition on Au surfaces; macroscopic events following Cu deposition; deposition of small metallic clusters on carbon; overpotential deposition of metals; underpotential deposition; STM on nanoscale ceramic superlattices; reconstruction events on Au(ijk) surfaces; Au surface reconstructions; friction force measurements on graphite steps under potential control; and the biocompatibility of materials.

Download or read book Probing Biological Interactions One Molecule at a Time written by and published by . This book was released on 2016 with total page 151 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Applied Scanning Probe Methods VIII written by Bharat Bhushan and published by Springer Science & Business Media. This book was released on 2007-12-20 with total page 512 pages. Available in PDF, EPUB and Kindle. Book excerpt: The volumes VIII, IX and X examine the physical and technical foundation for recent progress in applied scanning probe techniques. This is the first book to summarize the state-of-the-art of this technique. The field is progressing so fast that there is a need for a set of volumes every 12 to 18 months to capture latest developments. These volumes constitute a timely comprehensive overview of SPM applications.

Download or read book Fundamental Physics for Probing and Imaging written by Wade Allison and published by Oxford University Press. This book was released on 2006-10-26 with total page 349 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book addresses the question 'What is physics for?' Physics has provided many answers for mankind by extending his ability to see. Modern technology has enabled the power of physics to see into objects to be used in archaeology, medicine including therapy, geophysics, forensics and other spheres important to the good of society. The book looks at the fundamental physics of the various methods and how they are used by technology. These methods are magnetic resonance, ionising radiation and sound. By taking a broad view over the whole field it encourages comparisons, but also addresses questions of risk and benefit to society from a fundamental viewpoint. This textbook has developed from a course given to third year students at Oxford and is written so that it can be used coherently as a basis for shortened courses by omitting a number of chapters.

Download or read book Scanning Probe Microscopy and Spectroscopy written by Dawn Bonnell and published by John Wiley & Sons. This book was released on 2000-12-05 with total page 520 pages. Available in PDF, EPUB and Kindle. Book excerpt: A practical introduction to basic theory and contemporary applications across a wide range of research disciplines Over the past two decades, scanning probe microscopies and spectroscopies have gained acceptance as indispensable characterization tools for an array of disciplines. This book provides novices and experienced researchers with a highly accessible treatment of basic theory, alongside detailed examples of current applications of both scanning tunneling and force microscopies and spectroscopies. Like its popular predecessor, Scanning Probe Microscopy and Spectroscopy, Second Edition features contributions from distinguished scientists working in a wide range of specialties at university, commercial, and government research labs around the world. Chapters have been edited for clarity, conciseness, and uniformity of presentation to provide professionals with a concise working reference to scanning probe microscopic and spectroscopic principles, techniques, and practices. This Second Edition has been substantially revised and expanded to reflect important advances and new applications. In addition to numerous examples, the Second Edition features expanded coverage of electrostatic and magnetic force microscopies, near-field optical microscopies, and new applications of buried interfaces in nanomechanics, electrochemistry, and biology. Scanning Probe Microscopy and Spectroscopy, Second Edition is an indispensable working resource for surface scientists, microscopists, and spectroscopists in materials science, chemistry, engineering, biochemistry, physics, and the life sciences. It is also an unparalleled reference text for advanced undergraduates and graduate students in those fields.

Download or read book Scanning Probe Microscopy in Nanoscience and Nanotechnology 2 written by Bharat Bhushan and published by Springer Science & Business Media. This book was released on 2010-12-17 with total page 823 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents the physical and technical foundation of the state of the art in applied scanning probe techniques. It constitutes a timely and comprehensive overview of SPM applications. The chapters in this volume relate to scanning probe microscopy techniques, characterization of various materials and structures and typical industrial applications, including topographic and dynamical surface studies of thin-film semiconductors, polymers, paper, ceramics, and magnetic and biological materials. The chapters are written by leading researchers and application scientists from all over the world and from various industries to provide a broader perspective.

Download or read book Scanning Probe Microscopy of Soft Matter written by Vladimir V. Tsukruk and published by John Wiley & Sons. This book was released on 2012-01-09 with total page 663 pages. Available in PDF, EPUB and Kindle. Book excerpt: Well-structured and adopting a pedagogical approach, this self-contained monograph covers the fundamentals of scanning probe microscopy, showing how to use the techniques for investigating physical and chemical properties on the nanoscale and how they can be used for a wide range of soft materials. It concludes with a section on the latest techniques in nanomanipulation and patterning. This first book to focus on the applications is a must-have for both newcomers and established researchers using scanning probe microscopy in soft matter research. From the contents: * Atomic Force Microscopy and Other Advanced Imaging Modes * Probing of Mechanical, Thermal Chemical and Electrical Properties * Amorphous, Poorly Ordered and Organized Polymeric Materials * Langmuir-Blodgett and Layer-by-Layer Structures * Multi-Component Polymer Systems and Fibers * Colloids and Microcapsules * Biomaterials and Biological Structures * Nanolithography with Intrusive AFM Tipand Dip-Pen Nanolithography * Microcantilever-Based Sensors

Download or read book Handbook of Imaging in Biological Mechanics written by Corey P. Neu and published by CRC Press. This book was released on 2014-10-24 with total page 579 pages. Available in PDF, EPUB and Kindle. Book excerpt: Emerging imaging techniques have opened new fronts to investigate tissues, cells, and proteins. Transformative technologies such as microCT scans, super-resolution microscopy, fluorescence-based tools, and other methods now allow us to study the mechanics of cancer, dissect the origins of cellular force regulation, and examine biological specimens

Download or read book Single Molecule Tools Part A Fluorescence Based Approaches written by and published by Academic Press. This book was released on 2010-08-17 with total page 559 pages. Available in PDF, EPUB and Kindle. Book excerpt: Single molecule tools have begun to revolutionize the molecular sciences, from biophysics to chemistry to cell biology. They hold the promise to be able to directly observe previously unseen molecular heterogeneities, quantitatively dissect complex reaction kinetics, ultimately miniaturize enzyme assays, image components of spatially distributed samples, probe the mechanical properties of single molecules in their native environment, and "just look at the thing" as anticipated by the visionary Richard Feynman already half a century ago. Single Molecule Tools, Part A: Fluorescence Based Approaches captures a snapshot of this vibrant, rapidly expanding field, presenting articles from pioneers in the field intended to guide both the newcomer and the expert through the intricacies of getting single molecule tools. - Includes time-tested core methods and new innovations applicable to any researcher employing single molecule tools - Methods included are useful to both established researchers and newcomers to the field - Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines

Download or read book Handbook of Clinical Nanomedicine written by Raj Bawa and published by CRC Press. This book was released on 2016-02-22 with total page 1708 pages. Available in PDF, EPUB and Kindle. Book excerpt: This handbook (55 chapters) provides a comprehensive roadmap of basic research in nanomedicine as well as clinical applications. However, unlike other texts in nanomedicine, it not only highlights current advances in diagnostics and therapeutics but also explores related issues like nomenclature, historical developments, regulatory aspects, nanosim

Download or read book Photoionization and Other Probes of Many Electron Interactions written by F. Wuilleumier and published by Springer Science & Business Media. This book was released on 2013-11-21 with total page 474 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Advanced Study Institute on "Photoionization and Other Probes of Many-Electron Interactions" was held at the Centre "Les Cigales" in Carry-Ie-Rouet (France), from August 31st till September 13th 1975. The Institute was sponsored by the Scienti fic Affairs Division of NATO. The "Centre National de la Recher che Scientifique" (France) gave also partial support to the French participants and the National Science Foundation (U. S . A. ) to the American participants. A total of 18 lecturers, and 54 students selected among more than 120 applicants, attended the Institute. Over the last few years, substantial progress has been made in the experimental study of photon- or electron interactions with atoms. In particular, the g. rowing number of facilities created to use the synchrotron radiation makes now possible the realization of new types of experiments. The accumulation of new results showed clearly it was necessary to introduce electron correlations in the theoretical models in order to explain the existence and the probability of a large number of processes, in particular multiple processes. Thus large progress has also been made in the theore tical description of the excitation of the electronic systems and their interactions. It was the purpose of this Institute to bring together theoreticians and experimentalists in order to provide an opportunity to present in details the state of the art, in experiment as well as in theory, and to favor discussions on future experimen tal and theoretical studies.

Download or read book Handbook of Lipid Membranes written by Cyrus R. Safinya and published by CRC Press. This book was released on 2021-09-14 with total page 371 pages. Available in PDF, EPUB and Kindle. Book excerpt: This handbook provides a unique overview of lipid membrane fundamentals and applications. The fascinating world of lipids that harbor and govern so many biological functionalities are discussed within the context of membrane structures, interactions, and shape evolution. Beyond the fundamentals in lipid science, this handbook focuses on how scientists are building bioinspired biomimetic systems for applications in medicine, cosmetics, and nanotechnology. Key Features: Includes experimental and theoretical overviews on the role of lipids, with or without associated biomolecules, as structural components imparting distinct membrane shapes and intermembrane interactions Covers the mechanisms of lipid-membrane curvature, by peptide and protein binding, and the roles of signalling lipids and the cytoskeleton in plasma membrane shape evolution Covers advanced X-ray and force measurement techniques Discusses applications in biomedicine, cosmetics, and nanotechnology, including lipid vectors in nucleic acid, drug delivery in dermal applications, and lipid-based sensors and artificial biointerfaces Covers artificial membranes from block copolymers, synthetic copolypeptides, and recombinant proteins Includes an exciting section that explores the role of lipids in the origin of life in hydrothermal conditions This book is a highly informative companion for professionals in biophysics, biochemistry, physical chemistry, and material and pharmaceutical sciences and bioengineering.