Download or read book Preparation and Characterisation of Mammalian Plasma Membranes written by W. Howard Evans and published by . This book was released on 1978 with total page 266 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Preparation and Mammalian Plasma Membranes written by and published by . This book was released on 1979 with total page 522 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Preparation and Mammalian Plasma Membranes written by and published by Elsevier. This book was released on 1979-01-15 with total page 531 pages. Available in PDF, EPUB and Kindle. Book excerpt: Within recent years, affinity chromatography has become established as one of the most potent separatory techniques available to the biochemist. The technique has been exploited in almost every area of biochemistry and its applications are rapidly infiltrating cellular biology, immunology, medicine and technology. Because of this explosive development, this book is not intended as a comprehensive account of all the available systems but rather as a guide to current trends. However, whilst the detailed methodology of these systems vary, they are based on common principles.The aim of this book is to delineate these common principles and show how they are applied in practice.
Download or read book Preparation and Characterisation of Mammalian Plasma Membranes written by W. Howard Evans and published by . This book was released on 1979 with total page 522 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Preparation and Characterisation of Mammalian Plasma Membranes Part 1 written by W. Howard Evans and published by . This book was released on 1979 with total page 522 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Methods in Membrane Biology written by Edward D. Korn and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 260 pages. Available in PDF, EPUB and Kindle. Book excerpt: Volume 3 continues the approach carried out in the first two volumes of this se ries of publishing articles on membrane methodology which include, in addition to procedural details, incisive discussions of the ap plications of the methods and of their limitations. Wh at is the theoretical basis of the method, how and to what problems can it be applied, how does one interpret the results, what has thus far been achieved by the method, what lies in the future-these are the questions the authors have tried to answer. No area of membrane biology engages the interest of more investigators than studies of the plasma membrane. Four chapters in this volume are concerned with one or more aspects of the cell surface. Fundamental to all studies of the cell surface are the isolation and characterization of pure plasma membranes. Many preparations described in the literature are inadequate or are inadequately characterized. In the first chapter, Neville discusses the theoretical and practical bases of tissue fractionation, empha sizes the variations in enzyme content among plasma membranes from different sources, offers guidance in the choice of the proper criteria for assessing membrane purity, and suggests the best markers for detecting the possible presence of contaminating organelles. To review in detail each of the many preparations of plasma membranes that have been published is impossible.
Download or read book Laboratory Techniques in Biochemistry and Molecular Biology written by Elizabeth Work and published by . This book was released on 1978 with total page 266 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Laboratory Techniques in Biochemistry and Molecular Biology written by Thomas Spence Work and published by . This book was released on 1978 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Laboratory Techniques in Biochemistry and Molecular Biology written by T. S. Work and published by . This book was released on 1970 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Laboratory techniques in biochemistry and molecular biology written by E. Work and published by . This book was released on 1979 with total page 522 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Methods in Membrane Biology written by Edward D. Korn and published by Springer. This book was released on 1975-03-01 with total page 246 pages. Available in PDF, EPUB and Kindle. Book excerpt: Volume 3 continues the approach carried out in the first two volumes of this se ries of publishing articles on membrane methodology which include, in addition to procedural details, incisive discussions of the ap plications of the methods and of their limitations. Wh at is the theoretical basis of the method, how and to what problems can it be applied, how does one interpret the results, what has thus far been achieved by the method, what lies in the future-these are the questions the authors have tried to answer. No area of membrane biology engages the interest of more investigators than studies of the plasma membrane. Four chapters in this volume are concerned with one or more aspects of the cell surface. Fundamental to all studies of the cell surface are the isolation and characterization of pure plasma membranes. Many preparations described in the literature are inadequate or are inadequately characterized. In the first chapter, Neville discusses the theoretical and practical bases of tissue fractionation, empha sizes the variations in enzyme content among plasma membranes from different sources, offers guidance in the choice of the proper criteria for assessing membrane purity, and suggests the best markers for detecting the possible presence of contaminating organelles. To review in detail each of the many preparations of plasma membranes that have been published is impossible.
Download or read book Laboratory Techniques in Biochemistry and Molecular Biology written by Thomas Spence Work and published by . This book was released on 1979 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Molecular Biology of The Cell written by Bruce Alberts and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Membrane Protein Protocols written by Barry S. Selinsky and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 330 pages. Available in PDF, EPUB and Kindle. Book excerpt: Knowledge of the three-dimensional structure of a protein is absolutely required for the complete understanding of its function. The spatial orientation of amino acids in the active site of an enzyme demonstrates how substrate specificity is defined, and assists the medicinal chemist in the design of s- cific, tight-binding inhibitors. The shape and contour of a protein surface hints at its interaction with other proteins and with its environment. Structural ana- sis of multiprotein complexes helps to define the role and interaction of each individual component, and can predict the consequences of protein mutation or conditions that promote dissociation and rearrangement of the complex. Determining the three-dimensional structure of a protein requires milligram quantities of pure material. Such quantities are required to refine crystallization conditions for X-ray analysis, or to overcome the sensitivity limitations of NMR spectroscopy. Historically, structural determination of proteins was limited to those expressed naturally in large amounts, or derived from a tissue or cell source inexpensive enough to warrant the use of large quantities of cells. H- ever, with the advent of the techniques of modern gene expression, many p- teins that are constitutively expressed in minute amounts can become accessible to large-scale purification and structural analysis.
Download or read book Membrane Protein Structure and Function Characterization written by Jean-Jacques Lacapere and published by Humana Press. This book was released on 2017-08-10 with total page 419 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this present volume, different approaches are detailed to produce membrane proteins, purify them, study their function, determine their structure, and model them in membrane. Since every membrane protein behaves mostly in a unique way /fashion, knowledge of guidelines and tricks may help to increase chances to express, purify and characterize a peculiar membrane protein. Production of correctly folded protein remains a challenge. Moreover, getting a functional and stable protein requires to optimize membrane mimicking environments that can be detergent or artificial membranes. In some cases, the finding of the correct ligand which will stabilize the desired conformation is needed. In other cases, stabilization can be obtained using specific antibodies. This volume also presents different techniques to analyze the functional status of membrane proteins. Written in the highly successful Methods in Molecular Biology series format, chapters in Membrane Protein Structure and Function Characterization: Methods and Protocols provide different techniques to analyze the functional and structural status of membrane proteins. Chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Membrane Protein Structure and Function Characterization: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Download or read book Brush Border Membranes written by Ruth Porter and published by John Wiley & Sons. This book was released on 2009-09-14 with total page 192 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Novartis Foundation Series is a popular collection of the proceedings from Novartis Foundation Symposia, in which groups of leading scientists from a range of topics across biology, chemistry and medicine assembled to present papers and discuss results. The Novartis Foundation, originally known as the Ciba Foundation, is well known to scientists and clinicians around the world.
Download or read book Insect Cell Cultures written by Just M. Vlak and published by Springer Science & Business Media. This book was released on 2006-04-11 with total page 303 pages. Available in PDF, EPUB and Kindle. Book excerpt: A comprehensive reference work covering the key issues in insect cell cultures, this text includes 30 review papers on such topics as: cell lines (development, characterisation, physiology, cultivation and medium design); viruses (virus-cell interactions, replication, recombinant construction, infection kinetics, post-translational modification and passage effects); engineering (shear, bioreactors including perfusion, immobilisation, scale-up and modelling, downstream processing); applications; and economics and regulatory aspects.; This text should be useful for cell biologists, biochemists, molecular biologists, virologists, immunologists and other basic and applied disciplines related to cell culture engineering, both academic and industrial.
