Download or read book Plant Microtechnique and Microscopy written by Steven E. Ruzin and published by Oxford University Press, USA. This book was released on 1999 with total page 322 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant microtechnique has generated renewed interest in recent years, due in part to the need for molecular biologists to visualize a gene or gene product in the context of the whole plant. Plant Microtechnique and Microscopy offers uniquely in-depth coverage of this reinvigorated field. Thoroughly covering classical aspects of microscope slide preparation, it goes a step beyond all other available manuals by also documenting the theory and practice of modern applications. The text opens with single-page "Quick Start" protocols that provide students with fundamental instructions to complete eight of the most common microtechnique protocols used today. The following sections cover the theory and practice of microtechnique. The traditional paraffin method is demonstrated by explicit step-by-step protocols, and theoretical background is incorporated to give students the tools required to design their own experiments and to interpret existing results. In addition, modern applications such as methacrylate embedding and sectioning, microwave tissue processing, fluorescence histochemistry, and in situ hybridization are discussed in detail. The manual also contains a definitive chapter on microscopy and describes, in both text and diagrams, the optical principles of techniques such as phase contrast and DIC as well as confocal and deconvolution wide-field microscopy. Appendices on laboratory practice (chemical toxicities, common calculations, and buffer tables), an extensive appendix on optics and its application to the microscope, and an extensive bibliography of over 550 references are also included. Ideal for courses in plant biology, Plant Microtechnique and Microscopy also serves as an indispensable reference for all students of microscopy, histology, and histological technique. It is a valuable addition to every biological laboratory.

Download or read book Botanical Microtechnique and Cytochemistry written by Graeme P. Berlyn and published by Iowa State Press. This book was released on 1976 with total page 338 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Botanical Microtechnique written by Albrecht Zimmermann and published by . This book was released on 1893 with total page 320 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Plant Microtechnique written by D. A. Jahansen and published by . This book was released on 1979 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Plant Microtechniques and Protocols written by Edward Chee Tak Yeung and published by Springer. This book was released on 2015-09-19 with total page 572 pages. Available in PDF, EPUB and Kindle. Book excerpt: A proper understanding of the structural organization of the plant body is essential to any study in plant biology. Experimental studies in vivo and in situ will lead to structural, physiological, and cellular changes of the experimental material. To study macroscopic and microscopic changes, different histological methods and microtechniques can be used as they provide valuable information of the experimental system. In addition, the observed structural changes allow investigators to set hypothesis for further studies based on one’s own observation. Thus, proper selection and utilization of microtechniques are a must for the success of a research program. At present, an up-to-date collection of protocols are not readily available in the literature. The latest work in plant microtechniques was published in 1999 by Ruzin but many others are no longer in print [e.g., Jensen (1964); O’Brien and McCully (1981)]. Furthermore, a majority of published works focus on techniques related to general processing and staining procedures. A comprehensive treatment that encompasses broader applications of microtechniques to other disciplines is lacking [e.g., archeology, wood science, etc.]. There is a need to create a comprehensive volume of botanical methods and protocols which includes traditional and novel techniques that can be used by researchers in plant science and investigators in other disciplines that require plant microtechniques in their research and teaching. This book covers a wide variety of applications and brings them up-to-date to make them understandable and relevant, especially to students using the methods for the first time. It is our intention to create a useful reference for plant histology and related methods that will serve as a foundation for plant scholars, researchers, and teachers in the plant sciences.​

Download or read book Elements of Botanical Microtechnique written by John E. Sass and published by Bente Press. This book was released on 2008-11 with total page 248 pages. Available in PDF, EPUB and Kindle. Book excerpt: ELEMENTS OF BOTANICAL MICROTECHNIQUE by JOHN E. SASS. PREFACE: Permanent slides for microscopic study are indispensable in the teaching of a basic course in botany and in some specialized advanced fields. In some advanced courses the students prefer many of the slides as a minor aspect of the course, but in elementary courses the slides usually are furnished. In the latter case the slides are either purchased from commercial sources or made in the departmental laboratory. Biological supply houses can furnish excellent slides of the subjects commonly used in elementary teaching, but the quality is likely to be variable, especially from concerns in which there is frequent turnover of the laboratory staff. Jobbing houses that purchase slides from constantly changing sources may also furnish disappointing slides at times. The more reputable concerns, however, try earnestly to meet the specifications of critical and reasonable purchasers. The relative merits of making slides and of purchasing them are in dispute and must be worked out in relation to local conditions Because of these uncertainties in the commercial supply and the need for specialized or unlisted items, the preparation of slides is an established service function in many biological departments. This work is often performed by a skilled professional technician with more or less supervision by the departmental staff. In other departments a member of the teaching staff, usually a morphologist, assumes this responsibility, with the aid of student assistants. Most research organizations maintain a technician for the preparation of research slides. There are many types of investigation in which it is possible for the technician to place the finished slides before the investigator, who then carries out the study and interpretation of the material. However, in many investigations some or all steps in the preparation require an intimate knowledge of the history, structure, and orientation of the material and the aims of the study...

Download or read book Botanical Microtechnique written by Albrecht Zimmermann and published by . This book was released on 2023-07-18 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a practical guide to the methods and techniques used for the microscopical investigation of plants. Written by Albrecht Zimmermann, a respected botanist and microscopist, the book provides detailed instructions for the preparation and staining of plant specimens for microscopic examination. With its comprehensive coverage of the subject, clear instructions, and abundant illustrations, this book is an essential reference for botanists, microscopists, and plant scientists. This work has been selected by scholars as being culturally important, and is part of the knowledge base of civilization as we know it. This work is in the "public domain in the United States of America, and possibly other nations. Within the United States, you may freely copy and distribute this work, as no entity (individual or corporate) has a copyright on the body of the work. Scholars believe, and we concur, that this work is important enough to be preserved, reproduced, and made generally available to the public. We appreciate your support of the preservation process, and thank you for being an important part of keeping this knowledge alive and relevant.

Download or read book Botanical Microtechnique and Cytochemistry written by Graeme P. Berlyn and published by Iowa State Press. This book was released on 1976 with total page 390 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Botanical Microtechnique written by Albrecht Zimmermann and published by . This book was released on 1893 with total page 324 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Plant Cell Tissue and Organ Culture written by Oluf Gamborg and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 367 pages. Available in PDF, EPUB and Kindle. Book excerpt: This manual provides all relevant protocols for basic and applied plant cell and molecular technologies, such as histology, electron microscopy, cytology, virus diagnosis, gene transfer and PCR. Also included are chapters on laboratory facilities, operation and management as well as a glossary and all the information needed to set up and carry out any of the procedures without having to use other resource books. It is especially designed for professionals and advanced students who wish to acquire practical skills and first-hand experience in plant biotechnology.

Download or read book Botanical Microtechnique written by Alfred Brooker Klugh and published by . This book was released on 1911 with total page 31 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Elements of Botanical Microtechnique written by John Eugene Sass and published by . This book was released on 1940 with total page 240 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Potanical Microtechnique written by John E. Sass and published by . This book was released on 1964 with total page 248 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Research Methodology In Plant Science written by P.S. Narayana and published by Scientific Publishers. This book was released on 2016-11-01 with total page 388 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book comprises of different chapters associated with methodology in Plant science (Botany), describing in a simple and comprehensive way. The importance of creativity and motivation in research, the planning and proposal of research project, the description of different techniques involved in research are described in an elaborate way. It also includes the sources/collection of scientific information, method of scientific report/paper/thesis writing etc. The book is also a source of different aspects of research methodology in plant science dealt with in a comprehensive manner tailored to the needs of postgraduate students/research scholars for easy understanding. The book is profusely illustrated. The different chapters described in the book include: Introduction, Microscopy, Plant micro-technique, Smear/Sqush technique, Plant tissue culture, Herbarium technique, Hydrogen ion concentration (pH), Centrifugation, Chromatography, Electrophoresis, Colorimetry, Spectro-photometry, Radio-isotopes in biology and Computers and their application in plant sciences. Chapters on Biostatistics, Biophysics and Bioinformatics have also been included to help the student in the statistical analysis of the results, physical principles involved in the operation of different instruments and basics of bioinformatics. We sincerely hope that this book helps to fill up the lacuna and provides what all that is needed about the research methods required for a scholar/student in plant sciences to pursue their higher studies.

Download or read book BOTANICAL MICROTECHNIQUE written by ALFRED BROOKER. KLUGH and published by . This book was released on 2018 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Botanical Microtechnique written by John E 1897-1971 Sass and published by Legare Street Press. This book was released on 2023-07-18 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is an essential resource for anyone studying plants at the microscopic level. Providing detailed instructions and illustrations, the author guides the reader through the techniques of botanical microtechnique, from sample preparation to slide mounting. Whether you're a botanist, a student, or simply interested in the natural world, this book is an invaluable guide for those who want to explore the world of plants in greater detail. This work has been selected by scholars as being culturally important, and is part of the knowledge base of civilization as we know it. This work is in the "public domain in the United States of America, and possibly other nations. Within the United States, you may freely copy and distribute this work, as no entity (individual or corporate) has a copyright on the body of the work. Scholars believe, and we concur, that this work is important enough to be preserved, reproduced, and made generally available to the public. We appreciate your support of the preservation process, and thank you for being an important part of keeping this knowledge alive and relevant.

Download or read book Botanical Microtechnique written by John E 1897-1971 Sass and published by Palala Press. This book was released on 2015-09-08 with total page 244 pages. Available in PDF, EPUB and Kindle. Book excerpt: This work has been selected by scholars as being culturally important, and is part of the knowledge base of civilization as we know it. This work was reproduced from the original artifact, and remains as true to the original work as possible. Therefore, you will see the original copyright references, library stamps (as most of these works have been housed in our most important libraries around the world), and other notations in the work. This work is in the public domain in the United States of America, and possibly other nations. Within the United States, you may freely copy and distribute this work, as no entity (individual or corporate) has a copyright on the body of the work.As a reproduction of a historical artifact, this work may contain missing or blurred pages, poor pictures, errant marks, etc. Scholars believe, and we concur, that this work is important enough to be preserved, reproduced, and made generally available to the public. We appreciate your support of the preservation process, and thank you for being an important part of keeping this knowledge alive and relevant.