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Book Using Genetic Tools to Inform Management and Study Local Adaptation in Pacific Salmon

Download or read book Using Genetic Tools to Inform Management and Study Local Adaptation in Pacific Salmon written by Wesley Alan Larson and published by . This book was released on 2015 with total page 187 pages. Available in PDF, EPUB and Kindle. Book excerpt: Genetic analysis represents a powerful tool for informing management and studying adaptation in wild populations. For example, genetic tools can be used to delineate conservation units, assign individuals of unknown ancestry back to their populations of origin, and identify genes that are important for local adaptation. The overall goal of my thesis was to apply genetic tools to improve population-specific management and identify the genetic basis of local adaptation in Pacific salmon. Pacific salmon (Oncorhynchus sp.) return to their natal spawning habitats with high fidelity, promoting the formation of distinct populations that are highly adapted to their local environment. Pacific salmon are also an extremely important economic, cultural, and subsistence resource. These characteristics make Pacific salmon ideal candidates for population-specific management and facilitate the study of local adaptation. My dissertation consists of six chapters divided into two major themes. The first three chapters focus on applied research questions aimed at developing and utilizing genetic tools to improve management of Chinook salmon (Oncorhynchus tshawytscha), and the last three chapters focus on understanding the genetic basis of local adaptation in sockeye salmon (Oncorhynchus nerka). In chapter one, we used an existing genetic baseline to elucidate the migration patterns of Chinook salmon in the marine environment. Chapters two and three explored the use of genomics in a management context, applying data from thousands of genetic markers to develop novel resources that will aid in the conservation of Chinook salmon from western Alaska. For chapter four, we investigated patterns of selection at the major histocompatibility complex (MHC) in populations of sockeye salmon from the Wood River basin in southwestern Alaska. In chapter five, we constructed a genetic linkage map and conducted QTL analysis in five families of sockeye salmon. Finally, in chapter six we merged the linkage map with population data to study the genomic basis of adaptive divergence among three ecotypes of sockeye salmon from the Wood River basin. Taken together, these studies highlight the utility of genetic tools, especially genomics, for improving management and studying local adaptation in Pacific salmon.

Book Genetic Sex of Chinook Salmon in the Columbia River Basin

Download or read book Genetic Sex of Chinook Salmon in the Columbia River Basin written by James J. Nagler and published by . This book was released on 2004 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: It has previously been documented that naturally spawning, female fall-run chinook salmon (Oncorhynchus tshawytscha) from the Hanford Reach on the Columbia River carried a male-linked genetic marker, OtY1. The primary objective of this study was to investigate the spatial and temporal nature of this observation by sampling adults from the Hanford Reach and two other new sites, the lower Yakima River and at Ives Island below Bonneville Dam, in sequential years (2001 and 2002). A hatchery population on the Columbia River (Priest Rapids Hatchery) was sampled too. A total of 364 male and 359 female fall-run chinook salmon were sampled over the two spawning seasons from all sites. Detection of the male-linked genetic marker OtY1 in male fish was found to be 100% consistent with the phenotypic male sex in all populations sampled. A variable proportion of females from all sites tested positive for OtY1. This ranged from 67% at Ives Island in 2001 to 0% at the Priest Rapids Hatchery in 2002. A significant reduction of OtY1 incidence in females sampled in 2002 compared to 2001 emerged at all sites. A second objective sought evidence that a YY genotype might exist in the populations sampled. A real-time PCR method based on the growth hormone pseudogene (GHp), another male-linked chinook salmon genetic marker, was developed and tested to determine whether a YY genotype might be detected. The experimental variability between different XY individuals precluded its use to discriminate an YY from an XY genotype. During associated work on new chinook salmon sex linked genetic markers a new Y chromosome linked genetic marker, OtY2, was discovered.

Book Natural origin Chinook Salmon and Steelhead Life History and Genetic Diversity at PIT Tag Detection Locations Throughout the Snake River Basin

Download or read book Natural origin Chinook Salmon and Steelhead Life History and Genetic Diversity at PIT Tag Detection Locations Throughout the Snake River Basin written by John S. Hargrove and published by . This book was released on 2019 with total page 54 pages. Available in PDF, EPUB and Kindle. Book excerpt: This report summarizes life history and genetic diversity information for wild adult steelhead and spring/summer Chinook Salmon sampled at Lower Granite Dam and later detected in a Distinct Population Segment (DPS) or Evolutionarily Significant Unit (ESU) population in the Snake River basin for the 01/01/2018 to 12/31/2018 reporting period. This reporting period covers analysis of individuals crossing Lower Granite Dam in spawn year 2018. A total of 2,306 steelhead and 1,372 Chinook Salmon were sampled at Lower Granite Dam, 905 and 704 of which were subsequently identified at a PIT tag detection location elsewhere in the Snake River basin. Panels of up to 379 SNPs were genotyped at both Idaho Department of Fish and Game's Eagle Fish Genetics Lab and its collaborating laboratory, the Columbia River Inter-Tribal Fish Commission's Hagerman Genetics Lab, to assign these fish to hatchery parents or wild genetic stocks. We describe the life history variation and genetic diversity of steelhead and Chinook Salmon detected in Snake River populations. The information presented in this report provides critical data for viable Salmonid population monitoring of the Snake River steelhead DPS and the Snake River spring/summer Chinook Salmon ESU.

Book Population Genetic Structure and Life History Variability in Oncorhynchus Nerka from the Snake River Basin  Final Report

Download or read book Population Genetic Structure and Life History Variability in Oncorhynchus Nerka from the Snake River Basin Final Report written by and published by . This book was released on 2006 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The authors used protein electrophoresis to examine genetic relationships among samples of sockeye salmon and kokanee (Oncorhynchus nerka) from the Snake River basin. A few collections from elsewhere in the Pacific Northwest were also included to add perspective to the analysis. After combining temporal samples that did not differ statistically within and between years, 32 different populations were examined for variation at 64 gene loci scored in all populations. Thirty-five (55%) of these gene loci surveyed were polymorphic in at least one population. Average heterozygosities were relatively low (0.006--0.041), but genetic differentiation among populations was pronounced: the value of Wright's F[sub ST] of 0.244 is higher than has been reported in any other study of Pacific salmon.

Book Natural origin Chinook Salmon and Steelhead Life History and Genetic Diversity at PIT Tag Detection Locations Throughout the Snake River Basin

Download or read book Natural origin Chinook Salmon and Steelhead Life History and Genetic Diversity at PIT Tag Detection Locations Throughout the Snake River Basin written by John S. Hargrove and published by . This book was released on 2021 with total page 37 pages. Available in PDF, EPUB and Kindle. Book excerpt: This report summarizes life history and genetic diversity information for wild adult steelhead and spring/summer Chinook Salmon sampled at Lower Granite Dam and later detected in a Distinct Population Segment (DPS) or Evolutionarily Significant Unit (ESU) population in the Snake River basin for the 01/01/2020 to 12/31/2020 reporting period. This reporting period covers analysis of individuals crossing Lower Granite Dam in spawn year (SY) 2020. A total of 2,301 steelhead but no Chinook Salmon were sampled at Lower Granite Dam and 1,138 steelhead were subsequently identified at PIT tag detection locations in the Snake River basin. Panels of up to 379 SNPs were genotyped at both Idaho Department of Fish and Game’s Eagle Fish Genetics Lab and the Columbia River Inter-Tribal Fish Commission’s Hagerman Genetics Lab, to assign these fish to hatchery parents or wild genetic stocks. We describe the life history variation and genetic diversity of steelhead detected in Snake River populations for SY2020. The information presented in this report provides critical data for viable Salmonid population monitoring of the Snake River steelhead DPS and the Snake River spring/summer Chinook Salmon ESU.

Book Genetic Baseline Study for Selected Fish Species  Study Plan Section 9 14  Initial Study Report

Download or read book Genetic Baseline Study for Selected Fish Species Study Plan Section 9 14 Initial Study Report written by and published by . This book was released on 2014 with total page 164 pages. Available in PDF, EPUB and Kindle. Book excerpt: Tissue samples suitable for genetic analyses are being collected from fish species in the Susitna River basin, Alaska, for conducting genetic analysis of chinook salmon, and establishing tissue repositories for all four other salmon species and 20 non-salmon fish species. Chinook salmon tissue will be used to characterize genetic population structure, examine genetic variation for use in mixed-stock analysis, and, if sufficient variation is found, estimate the contribution of chinook salmon originating upstream of Devils Canyon to select habitats in the lower River. Salmon and non-salmon tissue repositories will be used for future studies to characterize the genetic legacy and variation of species and populations of interest. This document details Alaska Energy Authority's status in implementing the study as set forth in the FERC-approved Revised study plan and as modified by FERC's Study plan determination issued February 1, 2013.

Book Analysis of Factors Influencing the Population Dynamics of Chinook Salmon  Oncorhynchus Tshawytscha  in Central California

Download or read book Analysis of Factors Influencing the Population Dynamics of Chinook Salmon Oncorhynchus Tshawytscha in Central California written by Robert Glenn Kope and published by . This book was released on 1987 with total page 252 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Book Genetic History of Chinook and Sockeye Salmon Analyzed Using Ancient and Contemporary Mitochondrial DNA

Download or read book Genetic History of Chinook and Sockeye Salmon Analyzed Using Ancient and Contemporary Mitochondrial DNA written by Bobbi May Johnson and published by . This book was released on 2016 with total page 277 pages. Available in PDF, EPUB and Kindle. Book excerpt: Pacific salmon (Oncorhynchus spp.) serve an important social and economic role in western North America. Despite historical abundance, native salmonids are now at risk of extinction throughout much of their native range. The accurate characterization of historic range, population size, and gene flow is essential for the development of successful conservation strategies. Therefore, conservation disciplines may look to the past to inform the future. One framework for such investigations is phylogeography, which examines geographical and genealogical connections in an effort to understand the evolutionary history of organisms. Another avenue is the study of genetic data from temporally diverse samples, which facilitates the direct observation of a genetic history.

Book Genetic Baseline Study for Selected Fish Species  Study Plan Section 9 14  Initial Study Report

Download or read book Genetic Baseline Study for Selected Fish Species Study Plan Section 9 14 Initial Study Report written by and published by . This book was released on 2014 with total page 42 pages. Available in PDF, EPUB and Kindle. Book excerpt: Tissue samples suitable for genetic analyses are being collected from fish species in the Susitna River basin, Alaska, for conducting genetic analysis of chinook salmon, and establishing tissue repositories for all four other salmon species and 20 non-salmon fish species. Chinook salmon tissue will be used to characterize genetic population structure, examine genetic variation for use in mixed-stock analysis, and, if sufficient variation is found, estimate the contribution of chinook salmon originating upstream of Devils Canyon to select habitats in the lower River. Salmon and non-salmon tissue repositories will be used for future studies to characterize the genetic legacy and variation of species and populations of interest. This draft document reports on Alaska Energy Authority's status in implementing the study as set forth in the FERC-approved Revised study plan and as modified by FERC's Study plan determination issued February 1, 2013.

Book Chinook Salmon and Steelhead Genotyping for Genetic Stock Identification at Lower Granite Dam

Download or read book Chinook Salmon and Steelhead Genotyping for Genetic Stock Identification at Lower Granite Dam written by John S. Hargrove and published by . This book was released on 2020 with total page 25 pages. Available in PDF, EPUB and Kindle. Book excerpt: This report summarizes progress in the development and implementation of genetic stock identification (GSI) in the Snake River basin for natural-origin steelhead and spring/summer Chinook Salmon for the 01/01/2019 to 12/31/2019 reporting period. Four objectives for the GSI project are addressed in this report: 1) the maintenance and evaluation of single nucleotide polymorphism (SNP) panels for high-throughput genotyping of steelhead and Chinook Salmon in the Snake and Columbia river basins; 2) the updating, maintenance, and testing of SNP baselines to describe genetic variation and for use as a reference in conducting GSI for both species; 3) the implementation of GSI to estimate genetic stock composition and life history diversity of steelhead and spring/summer Chinook Salmon passing Lower Granite Dam (LGR); and 4) the summarization of life history and genetic diversity information for steelhead and spring/summer Chinook Salmon detected at PIT tag detection systems. For both species, panels of up to 379 SNPs have been in use for GSI and parentage-based tagging (PBT) at both Idaho Department of Fish and Game’s Eagle Fish Genetics Lab, and its collaborating laboratory, the Columbia River Inter-Tribal Fish Commission’s Hagerman Genetics Lab. Steelhead SNP baseline version v3.1 consists of 45 collections and 5,967 individuals. Chinook Salmon SNP baseline v3.1 consists of 30 collections and 4,356 individuals. SNP baselines are used to describe genetic diversity and structure of natural-origin populations throughout the Snake River. Based on population structure we have defined 10 genetic stocks for steelhead and 7 genetic stocks for Chinook Salmon for GSI analysis at LGR. We summarize GSI results for returning adults and emigrating juveniles during 2018-2019 at LGR using v3.1 baselines as reference. The information presented in this report provides critical data for viable salmonid population (VSP) monitoring of the Snake River steelhead DPS and the Snake River spring/summer Chinook Salmon ESU.

Book Chinook Salmon and Steelhead Genotyping for Genetic Stock Identification at Lower Granite Dam

Download or read book Chinook Salmon and Steelhead Genotyping for Genetic Stock Identification at Lower Granite Dam written by John S. Hargrove and published by . This book was released on 2021 with total page 80 pages. Available in PDF, EPUB and Kindle. Book excerpt: This report summarizes progress in the development and implementation of genetic stock identification (GSI) in the Snake River basin for natural-origin steelhead and spring/summer Chinook Salmon for the 01/01/2020 to 12/31/2020 reporting period. Four objectives for the GSI project are addressed in this report: 1) the maintenance and evaluation of single nucleotide polymorphism (SNP) panels for high-throughput genotyping of steelhead and Chinook Salmon in the Snake and Columbia river basins; 2) the updating, maintenance, and testing of SNP baselines to describe genetic variation and for use as a reference in conducting GSI for both species; 3) the implementation of GSI to estimate genetic stock composition and life history diversity of steelhead and spring/summer Chinook Salmon passing Lower Granite Dam (LGR); and 4) the summarization of life history and genetic diversity information for steelhead and spring/summer Chinook Salmon detected at PIT tag detection systems. For both species, panels of up to 368 SNPs have been in use for GSI and parentage-based tagging (PBT) at both Idaho Department of Fish and Game’s Eagle Fish Genetics Lab, and its collaborating laboratory, the Columbia River Inter-Tribal Fish Commission’s Hagerman Genetics Lab. We present an updated Steelhead SNP baseline version 4, which consists of 43 collections and 2,719 individuals. Chinook Salmon SNP baseline v3.1 consists of 30 collections and 4,356 individuals. SNP baselines are used to describe genetic diversity and structure of natural-origin populations throughout the Snake River. Based on population structure we have defined 10 genetic stocks for steelhead and 7 genetic stocks for Chinook Salmon for GSI analysis at LGR. We summarize GSI results for returning adults and emigrating juveniles during 2019-2020 at LGR using v3.1 baselines as reference for both steelhead and Chinook Salmon. Finally, we describe the life history variation and genetic diversity of steelhead and Chinook Salmon detected at IPTDS. The information presented in this report provides critical data for viable salmonid population (VSP) monitoring of the Snake River steelhead DPS and the Snake River spring/summer Chinook Salmon ESU.

Book Progress in Development of a DNA Baseline for Genetic Identification of Chinook Salmon Stocks of the Copper River Basin  Alaska

Download or read book Progress in Development of a DNA Baseline for Genetic Identification of Chinook Salmon Stocks of the Copper River Basin Alaska written by Lisa Wishard Seeb and published by . This book was released on 2006 with total page 11 pages. Available in PDF, EPUB and Kindle. Book excerpt: This report serves as a first-year summary of progress of a contract study intended to document the timing and origins of chinook salmon, Oncorhynchus tshawytscha, stocks in the Copper River and adjacent ocean fisheries using DNA markers. The project is intended to investigate the genetic structure of chinook salmon from the Copper River drainage using both microsatellite and single nucleotide polymorphisms (SNPs). Identification of genetic stocks of chinook salmon within the Copper River drainage and knowledge of their run timing and migration patterns will provide valuable information to optimize management and provide for sustainable fisheries. The first objective of this contract study is to develop a DNA database to delineate major geographic and temporal stocks of chinook salmon within the Copper River. This objective requires collection of tissue samples as well as the laboratory DNA analyses of those tissues. A second objective is to investigate run timing and entry patterns within the Copper River through the analysis of radio-tagged and fishwheel samples from Baird Canyon. A third objective is to characterize the timing of Copper River stocks in the ocean fisheries and estimate the contribution to this fishery by stocks of non-Copper River origin. Finally, these data will be standardized and contributed to a coastwide DNA database so that Copper River chinook salmon can be tracked throughout their marine migration. This report reviews the first year of the study which concentrated on objective 1.