Download or read book Polypeptide Materials with Engineered Functionalities written by Graciela E. Negri Jimenez and published by . This book was released on 2017 with total page 92 pages. Available in PDF, EPUB and Kindle. Book excerpt: Polypeptide materials were developed to address a variety of biomedical applications, including tissue engineering and drug delivery. This work was focused on increasing the pool of amino acid monomers to further the functionality and manipulation of polypeptide materials. This dissertation describes the development of polypeptide materials with stimuli-responsive behaviors and new functionality. A boronic acid-functionalized polypeptide-PEG copolymer was synthesized. This copolymer demonstrated the ability to reversibly bind to glycosylated enzymes; formed stable complexes at pH 7.4, and dissociated at pH 5.0, the pH of a lysosomal compartment. This complexation was specific to glycosylated enzymes and the boronic acid functionality used. These results suggest potential application of these copolymers in enzyme replacement therapies. A new L-lysine based -amino acid N-carboxyanhydride (NCA) monomer was synthesized in order to prepare photodegradable polypeptide hydrogels. The lysine derivative was caged using a UV-labile protecting group. Hydrogels were assembled using this residue as the hydrophobic block. Material properties could be adjusted by variation of hydrophobic block length and composition, as well as polymer concentration in water. These parameters were also used to tune the degradation profile of these hydrogels upon exposure to 365 nm light. Loading of dyes into these networks followed by irradiation demonstrated the ability for these gels to encapsulate and facilitate the triggered release of therapeutics. Finally, polysulfonium-based polypeptide hydrogels were synthesized, assembled and characterized using unnatural amino acid NCA monomers. Creation of these new materials relied on application of previously reported post-polymerization thioether alkylation methodology from the Deming lab to new building blocks. The resulting polymers were found to tolerate a range of functionality without loss of hydrogel properties. While hydrophobic block length and polymer concentration were used to tune gel properties, the choice of alkylating agent was also responsible for changing gel stiffness. Use of mixtures of different alkylating agents led to further fine adjustment of materials properties. The incorporation of azide groups was also tolerated by the scaffolds, and this functionality was used for subsequent click chemistry to demonstrate the retention of gel properties even after further functionalization. This methodology has implications for the incorporation of fluorescent labels or cell attachment-promoting ligands.
Download or read book Peptide Self Assembly and Engineering written by Xuehai Yan and published by John Wiley & Sons. This book was released on 2024-02-09 with total page 933 pages. Available in PDF, EPUB and Kindle. Book excerpt: Peptide Self-Assembly and Engineering State-of-the-art research in peptide self-assembly, with coverage of fundamental aspects of how peptides self-assemble and an extensive number of applications Peptide Self-Assembly and Engineering: Fundamentals, Structures, and Applications (2V set) covers the latest progresses in the field of peptide self-assembly and engineering, including the fundamental principles of peptide self-assembly, new theory of nucleation and growth, thermodynamics and kinetics, materials design rules, and precisely controlled structures and unique functions. The broad contents from this book enable readers to obtain a systematical and comprehensive knowledge in the field of peptide self-assembly and engineering. Contributed by the leading scientists and edited by a highly qualified academic and an authority in the field, Peptide Self-Assembly and Engineering includes information on: Emerging areas in peptide assembly, such as immune agents, bioelectronics, energy conversion, flexible sensors, biomimetic catalysis, and more Existing applications in biomedical engineering, nanotechnology, and photoelectronics, including tissue engineering, drug delivery, and biosensing devices History of peptide self-assembly for design of functional materials and peptides’ unique mechanical, optical, electronic, and biological properties Various solvent conditions, such as pH, ionic strength, and polarity, that can affect the structure and stability of peptide assemblies A very comprehensive reference covering the latest progresses in the field of peptide self-assembly and engineering, Peptide Self-Assembly and Engineering is an essential resource for all scientists performing research intersecting with the subject, including biochemists, biotechnologists, pharmaceutical chemists, protein chemists, materials scientists, and medicinal chemists.
Download or read book Peptide Materials written by Carlos Aleman and published by John Wiley & Sons. This book was released on 2013-03-29 with total page 479 pages. Available in PDF, EPUB and Kindle. Book excerpt: Peptides are the building blocks of the natural world; with varied sequences and structures, they enrich materials producing more complex shapes, scaffolds and chemical properties with tailorable functionality. Essentially based on self-assembly and self-organization and mimicking the strategies that occur in Nature, peptide materials have been developed to accomplish certain functions such as the creation of specific secondary structures (a- or 310-helices, b-turns, b-sheets, coiled coils) or biocompatible surfaces with predetermined properties. They also play a key role in the generation of hybrid materials e.g. as peptide-inorganic biomineralized systems and peptide/polymer conjugates, producing smart materials for imaging, bioelectronics, biosensing and molecular recognition applications. Organized into four sections, the book covers the fundamentals of peptide materials, peptide nanostructures, peptide conjugates and hybrid nanomaterials, and applications with chapters including: Properties of peptide scaffolds in solution and on solid substrates Nanostructures, peptide assembly, and peptide nanostructure design Soft spherical structures obtained from amphiphilic peptides and peptide-polymer hybrids Functionalization of carbon nanotubes with peptides Adsorption of peptides on metal and oxide surfaces Peptide applications including tissue engineering, molecular switches, peptide drugs and drug delivery Peptide Materials: From Nanostructures to Applications gives a truly interdisciplinary review, and should appeal to graduate students and researchers in the fields of materials science, nanotechnology, biomedicine and engineering as well as researchers in biomaterials and bio-inspired smart materials.
Download or read book Self Assembling Peptide Systems in Biology Medicine and Engineering written by A. Aggeli and published by Springer Science & Business Media. This book was released on 2007-05-08 with total page 364 pages. Available in PDF, EPUB and Kindle. Book excerpt: One ofthe major drivers in biological research is the establishment ofstructures and functions of the 50,000 or so proteins in our bodies. Each has a characteristic- dimensional structure, highly "ordered" yet "disordered"! This structure is essential for a protein's function and, significantly, it must be sustained in the competitive and complex environment of the living cell. It is now being recognised that when a cell loses control, proteins can se- assemble into more complex supermolecular structures such as the amyloid fibres and plaques associated with the pathogenesis of prion (CJD) or age-related (Alzheimer's) diseases. This is a pointer to the wider significance of the self-assembling properties of polypeptides. It has been long known that, in silk, polypeptides are assembled into- sheet structures which impart on the material its highly exploitable properties of flexibility combined with high tensile strength. But only now emerging is the recognition that peptides can Self-assemble into a wide variety of non-protein-like structures, including fibrils, fibres, tubules, sheets and monolayers. These are exciting observations and, more so, the potential for materials and medical exploitations is so wide ranging that over 80 scientists from Europe, USA, Japan and Israel. met 1-6 July 1999 in Crete, to discuss the wide-ranging implications of these novel developments. There was a spirit of excitement about the workshop indicative of an important new endeavor. The emerging perception is that of a new class of materials set to become commercially viable early in the 21st century.
Download or read book Peptides and Peptide based Biomaterials and their Biomedical Applications written by Anwar Sunna and published by Springer. This book was released on 2017-10-26 with total page 309 pages. Available in PDF, EPUB and Kindle. Book excerpt: Solid-binding peptides have been used increasingly as molecular building blocks in nanobiotechnology as they can direct the assembly and functionalisation of a diverse range of materials and have the ability to regulate the synthesis of nanoparticles and complex nanostructures. Nanostructured materials such as β-sheet fibril-forming peptides and α-helical coiled coil systems have displayed many useful properties including stimulus-responsiveness, modularity and multi-functionality, providing potential technological applications in tissue engineering, antimicrobials, drug delivery and nanoscale electronics. The current situation with respect to self-assembling peptides and bioactive matrices for regenerative medicine are reviewed, as well as peptide-target modeling and an examination of future prospects for peptides in these areas.
Download or read book Polypeptide Materials written by Maxim G. Ryadnov and published by Humana. This book was released on 2020-08-28 with total page 268 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book details the synthesis and assembly of polypeptide materials across length scales, i.e. proteins and peptides, their precursors, conjugates, and derivatives. A particular emphasis is made on measurement tools and procedures for material characterization, both physicochemical and functional. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Polypeptide Materials: Methods and Protocols serves to reflect the inter-disciplinary nature of molecular biology as well as the importance of developing innovative measurement methods to advance this vital area of research.
Download or read book Protein based Engineered Nanostructures written by Aitziber L. Cortajarena and published by Springer. This book was released on 2016-09-27 with total page 286 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is devoted to the engineering of protein-based nanostructures and nanomaterials. One key challenge in nanobiotechnology is to be able to exploit the natural repertoire of protein structures and functions to build materials with defined properties at the nanoscale using “bottom-up” strategies. This book addresses in an integrated manner all the critical aspects that need to be understood and considered to design the next generation of nano-bio assemblies. The book covers first the fundamentals of the design and features of the protein building blocks and their self-assembly illustrating some of the most relevant examples of nanostructural design. Finally, the book contains a section dedicated to demonstrated applications of these novel bioinspired nanostructures in different fields from hybrid nanomaterials to regenerative medicine. This book provides a comprehensive updated review of this rapidly evolving field.
Download or read book Peptide Applications in Biomedicine Biotechnology and Bioengineering written by Sotirios Koutsopoulos and published by Woodhead Publishing. This book was released on 2017-11-16 with total page 655 pages. Available in PDF, EPUB and Kindle. Book excerpt: Peptide Applications in Biomedicine, Biotechnology and Bioengineering summarizes the current knowledge on peptide applications in biomedicine, biotechnology and bioengineering. After a general introduction to peptides, the book addresses the many applications of peptides in biomedicine and medical technology. Next, the text focuses on peptide applications in biotechnology and bioengineering and reviews of peptide applications in nanotechnology. This book is a valuable resource for biomaterial scientists, polymer scientists, bioengineers, mechanical engineers, synthetic chemists, medical doctors and biologists. Presents a self-contained work for the field of biomedical peptides Summarizes the current knowledge on peptides in biomedicine, biotechnology and bioengineering Covers current and potential applications of biomedical peptides
Download or read book Functional Polypeptides for Biomedical Applications written by Jessica R. Kramer and published by . This book was released on 2012 with total page 230 pages. Available in PDF, EPUB and Kindle. Book excerpt: Synthetic polypeptides have shown great promise as materials for biotechnology and medicine, with applications in tissue engineering, drug delivery, and as therapeutics. Despite significant advances in the preparation of well-defined polypeptides and the development of self-assembling materials, a need remains for a broader scope of polypeptides with functionalities that mimic the complexity and function of post-translationally modified natural proteins. The display of functionalities that have therapeutic effects, target materials to specific tissues, passivate the immune response, or have stimuli responsive behavior, are highly desirable yet generally require complex and inefficient synthetic approaches. This dissertation reports several distinct advances in the preparation of a wide variety of highly functional polypeptide materials. Progress in both the polymerization of functionalized NCA monomers and the post-polymerization modification of polypeptides is described. A new method of NCA purification was developed, and has allowed access to diverse monomers with functionalities previously unattainable due to impurities that impeded polymerization. This purification technique was applied to the synthesis of glycosylated NCAs, which yielded the first living polymerizations of glycosylated NCAs and gave access to glycopolypeptides with unique properties and conformations. The display of sugar functionalities from synthetic polymers is an area of great interest due to the many attractive properties imparted upon the parent material, such as non-ionic water solubility, biological targeting, and shielding of the polypeptide from proteases. These glycopolypeptides were used to explore the effect of conformation on self-assembly and ligand binding, and to develop vesicles with potential medical applications in targeted drug delivery. In addition, a new conjugation technique for facile post-polymerization modification of polypeptides is described. This conjugation utilizes the unique chemistry of the natural amino acid methionine to allow chemoselective introduction of a wide variety of functional groups via alkylation. We investigated the stability of various alkylated methionines to thiolysis, and developed a simple method to attach and later remove different groups. Finally, work performed during an NSF-IGERT funded internship at HRL Laboratories Inc. is described. Surface modification of carbon foam anodes for microbial fuel cell applications was explored, and the use of thermogravimetric analysis as technique to evaluate biofilm formation was developed.
Download or read book Peptide Based Materials written by Timothy Deming and published by Springer Science & Business Media. This book was released on 2012-01-10 with total page 184 pages. Available in PDF, EPUB and Kindle. Book excerpt: Synthesis of Polypeptides by Ring-Opening Polymerization of α-Amino Acid N-Carboxyanhydrides, by Jianjun Cheng and Timothy J. Deming.- Peptide Synthesis and Self-Assembly, by S. Maude, L. R. Tai, R. P. W. Davies, B. Liu, S. A. Harris, P. J. Kocienski and A. Aggeli.- Elastomeric Polypeptides, by Mark B. van Eldijk, Christopher L. McGann, Kristi L. Kiick andJan C. M. van Hest.- Self-Assembled Polypeptide and Polypeptide Hybrid Vesicles: From Synthesis to Application, by Uh-Joo Choe, Victor Z. Sun, James-Kevin Y. Tan and Daniel T. Kamei.- Peptide-Based and Polypeptide-Based Hydrogels for Drug Delivery and Tissue Engineering, by Aysegul Altunbas and Darrin J. Pochan.-
Download or read book Peptide Based Materials written by Timothy Deming and published by Springer Science & Business Media. This book was released on 2012-01-13 with total page 184 pages. Available in PDF, EPUB and Kindle. Book excerpt: Synthesis of Polypeptides by Ring-Opening Polymerization of α-Amino Acid N-Carboxyanhydrides, by Jianjun Cheng and Timothy J. Deming.- Peptide Synthesis and Self-Assembly, by S. Maude, L. R. Tai, R. P. W. Davies, B. Liu, S. A. Harris, P. J. Kocienski and A. Aggeli.- Elastomeric Polypeptides, by Mark B. van Eldijk, Christopher L. McGann, Kristi L. Kiick andJan C. M. van Hest.- Self-Assembled Polypeptide and Polypeptide Hybrid Vesicles: From Synthesis to Application, by Uh-Joo Choe, Victor Z. Sun, James-Kevin Y. Tan and Daniel T. Kamei.- Peptide-Based and Polypeptide-Based Hydrogels for Drug Delivery and Tissue Engineering, by Aysegul Altunbas and Darrin J. Pochan.-
Download or read book Peptides and Proteins as Biomaterials for Tissue Regeneration and Repair written by Mario Barbosa and published by Woodhead Publishing. This book was released on 2017-09-25 with total page 392 pages. Available in PDF, EPUB and Kindle. Book excerpt: Peptides and Proteins as Biomaterials for Tissue Regeneration and Repair highlights the various important considerations that go into biomaterial development, both in terms of fundamentals and applications. After covering a general introduction to protein and cell interactions with biomaterials, the book discusses proteins in biomaterials that mimic the extracellular matrix (ECM). The properties, fabrication and application of peptide biomaterials and protein-based biomaterials are discussed in addition to in vivo and in vitro studies. This book is a valuable resource for researchers, scientists and advanced students interested in biomaterials science, chemistry, molecular biology and nanotechnology. Presents an all-inclusive and authoritative coverage of the important role which protein and peptides play as biomaterials for tissue regeneration Explores protein and peptides from the fundamentals, to processing and applications Written by an international group of leading biomaterials researchers
Download or read book Structural and Mechanistic Features of Genetically Engineered Polypeptides for Non biological Inorganic Materials written by John Laurence Kulp (III.) and published by . This book was released on 2006 with total page 281 pages. Available in PDF, EPUB and Kindle. Book excerpt: Technological advances have facilitated the generation of genetically engineered peptides and proteins that possess the capabilities of recognizing and binding to inorganic solids and/or controlling nucleation processes that form inorgnaic solids. However, very little is known regarding the structure of these interesting polypeptides and how structure contributes to functionality. This thesis presents studies done on three different genetically engineered peptides. First is a gold binding polypeptide (14 amino acids) that was selected to specifically bind gold {111} surfaces but has also been shown to nucleate gold crystals. Results show that the sequence binds two gold ions, one specifically in the -KTQATS motif and another nonspecifically at the C-terminal. Conformationally, the peptide possesses an open extended conformation available to adapt to the gold surface (at neutral pH). Next is an examination of a single-wall nanohorn binding peptide (12 amino acids). The conformation of this peptide, at neutral pH, is also in an open labile conformation that allows for adsorption onto the nanohorn surface. Repeats of these two peptides have shown to have a higher affinity for their respective surfaces and are also examined. The repeats show similarly unstructured states at neutral pH. Lastly is a genetically engineered protein p288 (116 amino acids), which catalyzes dendritic structures from both inorganic (NaCl, KCl, and CuSO4) and organic (sucrose) substrates. Again, this protein has a unstructured conformation at neutral pH. Studies of all three sequences (and some repeats) show that in the presence of trifluorethanol, a structure stabilizing solvent, that alpha-helical secondary structures are formed. This stabilization could play an important role in molecular assembly applications of these species. From a small genetically engineered peptide (12 amino acids) to a large genetically engineered protein (116 amino acids) there are conformationally similar features that must be owed to inherent ability to bind to and/or control the nucleation of inorganics.
Download or read book Biomaterials for Tissue Engineering Applications written by Jason A. Burdick and published by Springer Science & Business Media. This book was released on 2010-12-07 with total page 562 pages. Available in PDF, EPUB and Kindle. Book excerpt: A concise overview of tissue engineering technologies and materials towards specific applications, both past and potential growth areas in this unique discipline is provided to the reader. The specific area of the biomaterial component used within the paradigm of tissue engineering is examined in detail. This is the first work to specifically covers topics of interest with regards to the biomaterial component. The book is divided into 2 sections: (i) general materials technology (e.g., fibrous tissue scaffolds) and (ii) applications in the engineering of specific tissues (e.g., materials for cartilage tissue engineering). Each chapter covers the fundamentals and reflects not only a review of the literature, but also addresses the future of the topic. The book is intended for an audience of researchers in both industry and academia that are interested in a concise overview regarding the biomaterials component of tissue engineering, a topic that is timely and only growing as a field.
Download or read book Self Assembled Peptide Nanostructures written by Jaime Castillo and published by CRC Press. This book was released on 2012-11-21 with total page 326 pages. Available in PDF, EPUB and Kindle. Book excerpt: The self-organization of bionanostructures into well-defined functional machineries found in nature has been a priceless source of ideas for researchers. The molecules of life, proteins, DNA, RNA, etc., as well as the structures and forms that these molecules assume serve as rich sources of ideas for scientists or engineers who are interested in developing bio-inspired materials for innovations in biomedical fields. In nature, molecular self-assembly is a process by which complex three-dimensional structures with well-defined functions are constructed, starting from simple building blocks such as proteins and peptides. This book introduces readers to the theory and mechanisms of peptide self-assembly processes. The authors present the more common peptide self-assembled building blocks and discuss how researchers from different fields can apply self-assembling principles to bionanotechnology applications. The advantages and challenges are mentioned together with examples that reflect the state of the art of the use of self-assembled peptide building blocks in nanotechnology.
Download or read book Biomaterials Surface Science written by Andreas Taubert and published by John Wiley & Sons. This book was released on 2013-07-12 with total page 616 pages. Available in PDF, EPUB and Kindle. Book excerpt: At the interface of biology, chemistry, and materials science, this book provides an overview of this vibrant research field, treating the seemingly distinct disciplines in a unified way by adopting the common viewpoint of surface science. The editors, themselves prolific researchers, have assembled here a team of top-notch international scientists who read like a "who's who" of biomaterials science and engineering. They cover topics ranging from micro- and nanostructuring for imparting functionality in a top-down manner to the bottom-up fabrication of gradient surfaces by self-assembly, from interfaces between biomaterials and living matter to smart, stimuli-responsive surfaces, and from cell and surface mechanics to the elucidation of cell-chip interactions in biomedical devices. As a result, the book explains the complex interplay of cell behavior and the physics and materials science of artificial devices. Of equal interest to young, ambitious scientists as well as to experienced researchers.
Download or read book Biomimetic Protein Based Elastomers written by Namita Roy Choudhury and published by Royal Society of Chemistry. This book was released on 2022-04-20 with total page 297 pages. Available in PDF, EPUB and Kindle. Book excerpt: Elastomeric proteins are ubiquitous in nature, where they have evolved precise structures and properties that are necessary to perform specific biological roles and functions. This book emphasizes the impact of amino acid sequence on modulating protein structure, properties, and function. Examples include conformational ensemble dynamics, environmental responsiveness, self-assembly, physico-mechanical properties, morphology, and properties tailored for biomedical applications. This foundational framework is not only critical to advance scientific understanding and knowledge on elastomeric proteins but also enables the conceptualization, rational design, and development of biosynthetic elastomers and their analogous polypeptides for a variety of applications. Edited and contributed by pioneering researchers in the field, the book provides a timely overview of the materials, along with the synthesis techniques, the unique characteristics of elastomeric proteins, and biomedical and industrial applications. The book will provide a reference for graduate students and researchers interested in designing biomimetic proteins tailored for various functions.
