Download or read book Polymeric Microfluidic Devices for DNA Analysis written by Yi Sun and published by . This book was released on 2010 with total page 382 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Application of Polymeric Microfluidic Devices for Separation of Single stranded DNA written by Shawn Llopis and published by . This book was released on 2006 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Integrated Biochips for DNA Analysis written by Robin Liu and published by Springer Science & Business Media. This book was released on 2008-12-03 with total page 216 pages. Available in PDF, EPUB and Kindle. Book excerpt: The objective of this book is to provide up-to-date coverage of some of the emerging developments in the field of integrated DNA biochips. It will prove a useful source of information for researchers in the field and for those who are just entering the field of biochip research.
Download or read book Microfluidics for Advanced Functional Polymeric Materials written by Liang-Yin Chu and published by John Wiley & Sons. This book was released on 2017-03-21 with total page 459 pages. Available in PDF, EPUB and Kindle. Book excerpt: A comprehensive and systematic treatment of our current understanding of the microfluidic technique and its advantages in the controllable fabrication of advanced functional polymeric materials. Introducing and summarizing recent advances and achievements in the field, the authors cover the design and fabrication of microfluidic devices, the fundamentals and strategies for controllable microfluidic generation of multiphase liquid systems, and the use of these liquid systems with an elaborate combination of their structures and compositions for generating novel polymer materials, such as microcapsules, microfibers, valves, and membranes. Clear diagrams and illustrations throughout the text make the relevant theory and technologies more readily accessible. The result is a specialist reference for materials scientists, organic, polymer and physical chemists, and chemical engineers.
Download or read book Lab on a Chip written by R. Edwin Oosterbroek and published by Elsevier. This book was released on 2003-10-02 with total page 404 pages. Available in PDF, EPUB and Kindle. Book excerpt: I TECHNOLOGIES -- Hydrogels and polymers as components of a lab on a chip -- Microreplication technologies for polymer-based æTAS applications -- Silicon and glass micromachining for æTAS -- Surface chemistry in polymer microfluidic systems -- Plastic microfluidic devices: electrokinetic manipulations, life science applications, and production technologies -- II METHODS -- Transverse diffusion in microfluidic systems -- Nanoliter & picoliter liquid handling -- Micro sequential injection system for monitoring of metabolites extruded by cultured cells -- III CELL- & BEAD-BASED SYSTEMS -- Handling of beads in microfluidic devices for biotech applications -- Particles and molecules handling in micro channels -- Cell counting and cell sizing in microstructures -- IV APPLICATIONS -- Microfabricated capillary array electrophoresis: -- implementation and applications -- Microfluidic systems for analysis of the proteome with mass spectrometry -- Interfacing æTAS to matrix assisted laser desorpt ...
Download or read book Microfluidic Devices for Forensic DNA Analysis written by Jayna J. Shah and published by . This book was released on 2007 with total page 159 pages. Available in PDF, EPUB and Kindle. Book excerpt: "The development of integrated, miniaturized, and portable DNA analysis systems is crucial to alleviate massive backlog of unanalyzed samples and to address ever increasing demand for these assays. This thesis work contributes towards the development of a fully integrated microdevice capable of 'sample in -- answer out' for forensic DNA analysis. Specifically, this work describes the development of rapid and robust fabrication protocol for solvent-actuated bonding of polymeric thermoplastic substrates at room temperature, the development of microchannel wall coating strategies to eliminate analyte-wall interactions for high resolution separation of single-stranded DNA, and the characterization of a thin-film planar microwave transmission line for microfluidic heating applications. The solvent-actuated bonding protocol was based on the difference in capillary forces between the microchannel and the interstitial space between the surfaces of the two substrates to be bonded. This force differential wicked the bonding solvent into the gap between the substrates causing them to bond. The technique was implemented by placing the two substrates under moderate pressure, applying a moderate pneumatic vacuum to the fluidic channel, and introducing tens of microliter of bonding solvent through one end of the fluidic channel. The effect of bonding solvent on the dimensions of the microchannel was analyzed, and the mechanical robustness of the bonded devices was also characterized. Electrophoretic separation of single-stranded DNA (ssDNA) was successfully performed to demonstrate the functionality of these devices. To enhance ssDNA separation performance, schemes to modify poly(methyl methacrylate) (PMMA) -- the primary substrate used in this work -- were explored. This two step process consisted of altering surface hydrophilicity via surface activation using either nitric acid or UV/ozone followed by coating the surfaces with adsorptive polymers. Contact-angle measurements of the pristine and modified PMMA substrates were performed to quantify the change in wettability of the surface. Twofold increase in the separation efficiency was achieved by implementing these surface passivation strategies. Finally, the use of a thin-film planar microwave transmission line as a microwave power source was investigated for on-chip microwave heating of fluids. The microwave characterization data was used to develop a first-order analytic model of the microwave power absorption. The model was used to understand microwave power flow through the device and to calculate the fraction of the incident power absorbed in the fluid. Additionally, a fit of the predicted temperature obtained using this model to the measured temperature was performed to evaluate efficiency of this heating method"--Abstract.
Download or read book Microfluidics for Biological Applications written by Wei-Cheng Tian and published by Springer Science & Business Media. This book was released on 2009-03-02 with total page 429 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microfluidics for Biological Applications provides researchers and scientists in the biotechnology, pharmaceutical, and life science industries with an introduction to the basics of microfluidics and also discusses how to link these technologies to various biological applications at the industrial and academic level. Readers will gain insight into a wide variety of biological applications for microfluidics. The material presented here is divided into four parts, Part I gives perspective on the history and development of microfluidic technologies, Part II presents overviews on how microfluidic systems have been used to study and manipulate specific classes of components, Part III focuses on specific biological applications of microfluidics: biodefense, diagnostics, high throughput screening, and tissue engineering and finally Part IV concludes with a discussion of emerging trends in the microfluidics field and the current challenges to the growth and continuing success of the field.
Download or read book Forensic DNA Analysis written by Jaiprakash G. Shewale and published by CRC Press. This book was released on 2013-08-19 with total page 445 pages. Available in PDF, EPUB and Kindle. Book excerpt: The field of forensic DNA analysis has grown immensely in the past two decades and genotyping of biological samples is now routinely performed in human identification (HID) laboratories. Application areas include paternity testing, forensic casework, family lineage studies, identification of human remains, and DNA databasing. Forensic DNA Analysis:
Download or read book Microfluidic Techniques written by Shelley D. Minteer and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 245 pages. Available in PDF, EPUB and Kindle. Book excerpt: Hands-on researchers review the principles behind successful miniaturization and describe the key techniques for miniaturizing large-scale biochemical and bioanalytical methods for microchip analysis. The authors cover not only the most popular methods for the fabrication of microchips (photolithography, laser ablation, and soft lithography), but also microfluidic techniques for such bioanalytical assays and bioprocesses as DNA analysis, PCR, immunoassays, and cell reactors. Highlights include PCR on a microchip, microscale cell culturing, and the study of cellular processes on a microchip. The protocols offer step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Download or read book Simulating Deoxyribonucleic Acid Behavior in Microfluidic Devices written by Daniel Warner Trahan and published by . This book was released on 2010 with total page 115 pages. Available in PDF, EPUB and Kindle. Book excerpt: During most of the twentieth century, direct study of individual polymer molecules was impossible due to their small size. Therefore, polymers were typically studied in bulk solutions, and their behavior and interactions had to be understood through average bulk property measurements. Because the scale of most industrial applications greatly exceeded the size of these molecules, this level of analysis was satisfactory. In the last twenty years, however, the appearance of microfluidic devices, whose smallest length scales are comparable to the size of a polymer molecule, has offered ways to visually study the behavior of individual polymer molecules and made possible new and exciting applications that exploit the precise control afforded by the small size of these devices. One such application is gene mapping, which extracts, at a. coarse level, the information embedded in the base pair sequence of genomic DNA. This technology relies on the ability to manipulate single DNA molecules in order to perform such tasks as separating DNA based on length and stretching DNA away from its entropically coiled equilibrium state. Recently, many novel methods have been proposed to accomplish these tasks using microfabricated devices, and munch experimental work has been focused on identifying and characterizing the underlying physics governing these devices. Current understanding, however, is greatly hampered by the fact that experiments can only provide limited information about the behavior of DNA molecules (e.g., they are unable to resolve details on small time and length scales). Therefore, simulations are an invaluable tool in the study of DNA behavior in microfiuidic devices by complementing and guiding experimental investigations. In this thesis, we present Brownian Dynamics simulations of the single molecule behavior of DNA in microfluidic devices related to gene mapping. In particular, we have considered the use of a post array to "precondition" the configuration of molecules for subsequent stretching in a contraction and compared our results to previous experiments. We found good qualitative agreement between experiments and simulations for DNA behavior in the post array, but our simulations consistently overpredicted the final stretch of molecules at the end of the contraction, which we attributed to nonlinear electrokinetic effects. We also investigate the electrophoretic collision of a DNA molecule with a. large, ideally conducting post. Field-induced compression was shown to play a critical role in the escape process of a molecule trapped on the post surface, and an extensive theoretical analysis is performed, describing both the local field-induced compression and the larger collision problem. Finally, we study the relaxation process of an initially stretched molecule in slit-like confinement. We present the first simulation results that exhibit two distinct relaxation times in the linear force regime, as previously reported in recent experiments. Our analysis is focused on the experimentally inaccessible dynamics in the transverse directions, particularly at short times and on small length scales. Comparisons to the predictions of a recent mechanistic model of confined relaxation were found to be satisfactory.
Download or read book Microfluidic Lab on a Chip for Chemical and Biological Analysis and Discovery written by Paul C.H. Li and published by CRC Press. This book was released on 2005-11-01 with total page 529 pages. Available in PDF, EPUB and Kindle. Book excerpt: The microfluidic lab-on-a-chip allows scientists to conduct chemical and biochemical analysis in a miniaturized format so small that properties and effects are successfully enhanced, and processes seamlessly integrated. This microscale advantage translates into greater sensitivity, more accurate results, and better information. Microfluidic
Download or read book Microfluidic Technologies for Human Health written by Utkan Demirci and published by World Scientific. This book was released on 2012 with total page 495 pages. Available in PDF, EPUB and Kindle. Book excerpt: Ch. 1. A microscale bioinspired cochlear-like sensor / Robert D. White, Robert Littrell, and Karl Grosh -- ch. 2. Systematic evaluation of the efficiencies of proteins and chemicals in pharmaceutical applications / Morgan Hamon and Jong Wook Hong -- ch. 3. Microfluidic glucose sensors / Jithesh V. Veetil [und weitere] -- ch. 4. Applications of microfabrication and microfluidic techniques in mesenchymal stem cell research / Abhijit Majumder [und weitere] -- ch. 5. Patient-specific modeling of low-density lipoprotein transport in coronary arteries / Ufuk Olgac -- ch. 6. Point-of-care microdevices for global health diagnostics of infectious diseases / Sau Yin Chin [und weitere] -- ch. 7. Integrated microfluidic sample preparation for chip-based molecular diagnostics / Jane Y. Zhang [und weitere] -- ch. 8. Microfluidic devices for cellular proteomic studies / Yihong Zhan and Chang Lu -- ch. 9. Microfluidics for neuroscience: novel tools and future implications / Vivian M. Hernandez and P. Hande Ozdinler -- ch. 10. Microfluidics: on-chip platforms as in vitro disease models / Shan Gao, Erkin Seker, and Martin L. Yarmush -- ch. 11. Application of microfluidics in stem cell and tissue engineering / Sasha H. Bakhru, Christopher Highley, and Stefan Zappe -- ch. 12. Microfluidic "on-the-fly" fabrication of microstructures for biomedical applications / Edward Kang, Sau Fung Wong, and Sang-Hoon Lee -- ch. 13. Microfluidics as a promising tool toward distributed viral detection / Elodie Sollier and Dino Di Carlo -- ch. 14. Electrophoresis and dielectrophoresis for lab-on-a-chip (LOC) analyses / Yagmur Demircan, Gurkan Yilmaz, and Haluk Kulah -- ch. 15. Ultrasonic embossing of carbon nanotubes for the fabrication of polymer microfluidic chips for DNA sample purification / Puttachat Khuntontong, Min Gong, and Zhiping Wang -- ch. 16. Ferrofluidics / A. Rezzan Kose and Hur Koser -- ch. 17. Antibody-based blood bioparticle capture and separation using microfluidics for global health / ZhengYuan Luo [und weitere] -- ch. 18. Applications of quantum dots for fluorescence imaging in biomedical research / ShuQi Wang [und weitere]
Download or read book Fabrication of Polymer Based Microfluidic Devices for Chemical and Biological Analysis written by Bo Shen and published by . This book was released on 2016 with total page 104 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Fundamentals and Applications of Microfluidics Third Edition written by Nam-Trung Nguyen and published by Artech House. This book was released on 2019-01-31 with total page 567 pages. Available in PDF, EPUB and Kindle. Book excerpt: Now in its Third Edition, the Artech House bestseller, Fundamentals and Applications of Microfluidics, provides engineers and students with the most complete and current coverage of this cutting-edge field. This revised and expanded edition provides updated discussions throughout and features critical new material on microfluidic power sources, sensors, cell separation, organ-on-chip and drug delivery systems, 3D culture devices, droplet-based chemical synthesis, paper-based microfluidics for point-of-care, ion concentration polarization, micro-optofluidics and micro-magnetofluidics. The book shows how to take advantage of the performance benefits of microfluidics and serves as an instant reference for state-of-the-art microfluidics technology and applications. Readers find discussions on a wide range of applications, including fluid control devices, gas and fluid measurement devices, medical testing equipment, and implantable drug pumps. Professionals get practical guidance in choosing the best fabrication and enabling technology for a specific microfluidic application, and learn how to design a microfluidic device. Moreover, engineers get simple calculations, ready-to-use data tables, and rules of thumb that help them make design decisions and determine device characteristics quickly.
Download or read book Design of Polymeric Platforms for Selective Biorecognition written by Juan Rodríguez-Hernández and published by Springer. This book was released on 2015-08-21 with total page 388 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book addresses in an integrated manner all the critical aspects for building the next generation of biorecognition platforms - from biomolecular recognition to surface fabrication. The most recent strategies reported to create surface nano and micropatterns are thoroughly analyzed. This book contains descriptions of the types of molecules immobilized at surfaces that can be used for specific biorecognition, how to immobilize them, and how to control their arrangement and functionality at the surface. Small molecules, peptides, proteins and oligonucleotides are at the core of the biorecognition processes and will constitute a special part of this book. The authors include detailed information on biological processes, biomolecular screening, biosensing, diagnostic and detection devices, tissue engineering, development of biocompatible materials and biomedical devices.
Download or read book Polymer Analysis Polymer Theory written by Akihiro Abe and published by Springer Science & Business Media. This book was released on 2005-09-29 with total page 328 pages. Available in PDF, EPUB and Kindle. Book excerpt: This series presents critical reviews of the present and future trends in polymer and biopolymer science including chemistry, physical chemistry, physics and materials science. It is addressed to all scientists at universities and in industry who wish to keep abreast of advances in the topics covered. Impact Factor Ranking: Always number one in Polymer Science. More information as well as the electronic version of the whole content available at: www.springerlink.com
Download or read book Integrated Polymeric Surface Microfluidic Systems written by Thomas Henry Cauley (III.) and published by . This book was released on 2009 with total page 410 pages. Available in PDF, EPUB and Kindle. Book excerpt:
