Download or read book Pollen Cryopreservation Protocols written by P.E. Rajasekharan and published by Springer Nature. This book was released on 2023-02-20 with total page 605 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume focuses upon pollen cryopreservation in various crops from the most widely cultivated tomato to the lesser known medicinal species like Decalepis arayalpatra. Chapters detail standard techniques such as pollen collection, cryopreservation, pollen , germination tests for viability, and specific crops. Step-by-step protocols are complemented by personal notes, precautions, and specifying the reagents to be used in each step to ensure the repeatability of the procedure across labs. Authoritative and cutting-edge, Pollen Cryopreservation Protocols aims to serve as reference for researchers studying plant breeding and plant conservation biology.
Download or read book Comparison of 5 Pollen Storage Protocols for Subtropical Pine Species written by Michael Edward Tighe and published by . This book was released on 2005 with total page 96 pages. Available in PDF, EPUB and Kindle. Book excerpt: Keywords: in vitro germination, pollen cryopreservation, subtropical pines, pollen storage.
Download or read book Plant Cryopreservation A Practical Guide written by Barbara B.M. Reed and published by Springer Science & Business Media. This book was released on 2007-10-31 with total page 534 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cryopreservation has proven to be an important tool for the storage and conservation of plant genetic resources. This book is a unique resource for plant scientists, providing more than 100 ready-to-use cryopreservation protocols for plant types from algae and bryophytes to a range of flowering plants. It includes techniques for diverse plant parts such as dormant buds, pollen, and apical meristems and for cell types such as suspension and callus cultures.
Download or read book Comparison of 5 Pollen Storage Protocols for Subtropical Pine Species written by and published by . This book was released on 2004 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Storage of pine pollen for breeding and conservation purposes is essential to conserve valuable genetic material over time. Customized storage protocols are often necessary to address differing strategies and program goals for specific pine species. Subtropical pine pollen has been and will be important for forestry programs around the world, and the evaluation of pollen storage protocols for these species permits the determination of successful strategies to maintain viability over time. Germination of pollen grains using in vitro agar gel methodology has been shown to successfully predict in vivo germination of pollen tubes. Five methods commonly used for temperate pine pollen storage were evaluated for their efficacy for maintaining temperate (P. taeda, P. radiata) and subtropical species (Pinus tecunumanii, P. oocarpa, P. maximinoi) pollen after 6 months. For best results, cryopreservation at -196 & deg;C should be used for subtropical pine pollen storage if available. When reliable resources for cryopreservation are limited, direct freezing at -20 & deg;C is recommended. Like the P. taeda and P. radiata pollen evaluated in this study, subtropical pine pollen exhibited increased in vitro germination rates when exposed to the lower temperatures evaluated after 6 months in storage. Temperate species pollen storage protocols evaluated in this study were used successfully for subtropical and tropical pine species. The 3 subtropical pine species' pollen assessed diverged from temperate pollen behavior when only prestored pollen is considered. Cryogenic and direct freezing storage methods yielded higher in vitro germination rates for subtropical species than temperate species (P. taeda and P. radiata). Vacuum drying/freezing and refrigeration, however, yielded higher in vitro germination rates for temperate species. Additional research into the physiology of these differences and the correlations with evolutionary phylogeny information is recommended to determine the caus.
Download or read book Cryopreservation of Pollen written by W. James Hill Craddock and published by . This book was released on 1987 with total page 180 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cryopreservation of pollen offers a simple and effective method of long-term pollen storage. Pollen may be stored in liquid nitrogen (LN) for many years without loss of its essential capabilities to pollinate, fertilize, and set normal fruit and seed when used in plant breeding, for controlled pollinations, or for the conservation of plant genetic resources. Pollen of Corylus, Fragaria, Pyrus, Rubus and Vaccinium was frozen in LN either freshly collected, air dried, or after equilibration with atmospheres of 0, 20, 40, 60, 80, 90, or 100% relative humidity. Survival was assayed by in vitro germination on a sucrose-agar medium and by controlled pollinations (in Corylus only). All pollen survived freezing in LN provided the pollen moisture content was adjusted properly. Pollen of the wind-pollinated genus Corylus was not tolerant of desiccation and survived freezing at very high moisture content levels relative to the insect-pollinated Rosaceae pollens which survived freezing only when dried prior to their immersion in LN. Guidelines are presented for the establishment of a pollen bank at the National Clonal Germplasm Repository (NCGR) in Corvallis, using cryopreservation for the long-term conservation of plant genetic resources.
Download or read book Plant Cell Culture Protocols written by Víctor M. Loyola-Vargas and published by Humana Press. This book was released on 2012-05-10 with total page 430 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell culture methodologies have become standard procedures in most plant laboratories. Currently, facilities for in vitro cell cultures are found in practically every plant biology laboratory, serving different purposes since tissue culture has turned into a basic asset for modern biotechnology, from the fundamental biochemical aspects to the massive propagation of selected individuals. “Plant Cell Culture Protocols, Third Edition is divided into five convenient sections that cover topics from general methodologies, such as culture induction, growth and viability evaluation, statistical analysis and contamination control, to highly specialized techniques, such as clonal propagation, haploid production, somatic embryogenesis, organelle transformation. The volume concludes with a section on the laborious process of measuring the epigenetics changes in tissue cultures.”Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Plant Cell Culture Protocols, Third Edition seeks to serve both professionals and novices with its guide to the most common and applicable techniques and methods for plant tissue and cell culture.
Download or read book Cryopreservation and Freeze Drying Protocols written by John G. Day and published by Springer Science & Business Media. This book was released on 2007-06-05 with total page 703 pages. Available in PDF, EPUB and Kindle. Book excerpt: In addition to outlining the fundamental principles associated with the conservation of biological resources, freeze-drying and cryopreservation, this text is a compilation of cryptopreservation and freeze-drying methodologies applicable to different biological materiels, developed by expert laboratories.
Download or read book Biotechnology of Medicinal Plants with Antiallergy Properties written by Saikat Gantait and published by Springer Nature. This book was released on with total page 683 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Current Trends in Cryobiology written by Audrey U. Smith and published by Springer. This book was released on 2013-11-11 with total page 261 pages. Available in PDF, EPUB and Kindle. Book excerpt: During the past 20 years there have been amazing developments in low temperature physics, engineering, and biology. They form part of the very rapid post-war growth in pure and applied sciences of every kind. During this period several branches of biology including immunology, molecular biology and, of course, cryobiology, have split off from their parent disciplines. One result of this splintering has been the development of separate jargons used by the specialists and sometimes incomprehensible to those working in closely allied fields. The pure physicists, chemists, and the applied scientists, including physicians, surgeons, and pathologists, find the new jargons particularly baffling. We have attempted in this monograph to present to cryogenic engineers a picture of cryobiologists and their problems using as few strange technical words as possible. We hope that this book will help to bridge the gap which has already formed between them in spite of the opportunities for collaboration in many projects. We hope that it may also be useful to scientific research workers and postgraduate students of many kinds united only by curiosity about cryobiology. We are very much indebted to Dr. K. Mendelssohn, who insti gated us to produce a monograph and who reassured us that cryogenic engineers are as keen to understand the current trends in biology, and particularly in cryobiology, as we are to enlist their help. We have had much help in preparing this book.
Download or read book Heterosis Breeding in Vegetable Crops written by Nagendra Rai and published by New India Publishing. This book was released on 2006 with total page 564 pages. Available in PDF, EPUB and Kindle. Book excerpt: With reference to India.
Download or read book Conservation and Utilization of Horticultural Genetic Resources written by P.E. Rajasekharan and published by Springer. This book was released on 2019-06-25 with total page 680 pages. Available in PDF, EPUB and Kindle. Book excerpt: The conservation of crop genetic resources is one of the important elements in efforts to sustainably increase agricultural production in low-income countries, and to guarantee long-term food security, especially for the low-income population groups in these countries. Horticultural crops, as high-value crops, have an important role to play in revitalizing rural economies and can add significantly to national economies. Moreover, horticulture provides more than twice the number of jobs compared to traditional cereal crop production, and the shifting of conventional agriculture towards high-value horticulture has increased employment opportunities in developing countries. To exploit this potential, researchers need a vast array of horticultural genetic resources and information on new traits. Horticultural crops, which are only a part of PGRFA (Plant Genetic Resources for Food and Agriculture), are characterized by a wide and varied range of species. In fact, there are five major horticultural crop groups: fruit and nut crops, vegetables, food legumes, roots and tubers, and lastly the ornamental and medicinal group. In this context, the present book provides a comprehensive overview of the current state of conservation and utilization of horticultural genetic resources, addressing contemporary approaches to conservation in connection with different technologies, including biotechnological approaches as practised in India and in some cases, globally. It includes a brief chapter on the unique nature of horticultural genetic resources, providing a rationale for viewing them as being distinct from field crop genetic resources. Subsequent chapters share insights on protocols for the conservation of selected horticultural crops ex situ, and focus on the increased need to complement these efforts with in situ conservation approaches. Geospatial tools are also briefly described, emphasizing their utility with regard to mapping and managing resources. The book also explores the wild gene pool in horticulture crops; discusses legal aspects related to horticultural genetic resources and biotechnological aspects; and describes the key aspects of sustainable management and replenishment. Given its scope, the book offers a valuable resource for all horticulturists, graduate students, researchers, policymakers, conservationists, and NGOs engaged in horticulture in particular and biodiversity in general.
Download or read book Cryopreservation of Plant Germplasm II written by L.E. Towill and published by Springer Science & Business Media. This book was released on 2013-03-14 with total page 406 pages. Available in PDF, EPUB and Kindle. Book excerpt: Ex situ preservation of germplasm for higher plant species has been accom plished using either seeds or clones, but storage of these under typical condi tions does not provide the extreme longevities that are needed to minimize risk of loss. Costs of maintenance and regeneration of stocks are also high. Systems that provide virtually indefinite storage should supplement existing methods and it is within this context that cryopreservation is presented. The use of low temperature preservation was initially more a concern of medicine and animal breeding, and was expanded to plants in the 1970s. Sur vival after cryogenic exposure has now been demonstrated for diverse plant groups including algae, bryophytes, fungi and higher plants. If survival is com monplace, then the eventual application is a cryopreservation system, whereby cells, tissues and organs are held indefinitely for use, often in the unforeseen future. The increasing interest and capabilities for application could not have occurred at a more opportune time since expanding human populations have placed unprecedented pressures on plant diversity. This book emphasizes cry opreservation of higher plants and was initially driven by the concern for loss of diversity in crops and the recognized need that this diversity would be essential for continued improvement of the many plants used by society for food, health and shelter. The interest in cryopreservation has been expanded by conservationists and their concerns for retaining, as much as possible, the diversity of natural populations. The need for cryopreservation, thus, is well established.
Download or read book Cryopreservation written by Marian Quain and published by BoD – Books on Demand. This book was released on 2020-06-10 with total page 192 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cryopreservation - Current Advances and Evaluations sheds light on storage of cells at subzero temperatures while ensuring that biological functionality is not compromised. Cryopreservation presents a perfect technique by which life can be preserved for posterity. However, there are many challenges to overcome and questions to answer, such as: Are organisms and metabolic systems functioning normally after cooling and thawing? This book provides comprehensive information on cryopreservation with a particular focus on cryoprotectant agents (CPAs). CPAs prevent ice from forming on cryogenically preserved cells, tissues, and organs, but can become toxic at high concentrations. As such, more research is needed to determine their precise mechanisms of action and to develop potential new CPAs that will not compromise the biology of cells. This book is an attempt in this direction.
Download or read book The Coconut written by Steve Adkins and published by CABI. This book was released on 2024-01-25 with total page 252 pages. Available in PDF, EPUB and Kindle. Book excerpt: The coconut palm (Cocos nucifera L.) is one of the world's most important palms, and contributes significantly to the income and livelihood of many people in tropical countries. Widely referred to as the 'tree of life', coconut has been used as a source of food, drink, oil, medicine, shelter and wood for around 500 years. Every part of the coconut palm can be utilized. The demand for coconut fruit and its products has increased recently as people have become aware of its nutritional and health benefits, especially those of coconut water and virgin coconut oil. This book is a key resource for researchers and students in horticulture, plant science and agriculture, and those interested in the production of tropical crops, and practitioners in the coconut industry.
Download or read book A Protocol to Determine Seed Storage Behaviour written by T. D. Hong and published by Bioversity International. This book was released on 1996 with total page 62 pages. Available in PDF, EPUB and Kindle. Book excerpt: This publication provides an approach by which conservationists can determine whether or not long-term seed storage is feasible for a particular species, i.e. whether or not that species shows orthodox seed storage behaviour, and provides advice on the implementation of the protocol, examples of ways in which the results from seed storage studies could be misinterpreted due to confounding factors, as well as several alternative approaches for estimating seed storage behaviour prior to carryng out actual investigations with the seeds. In particular, the latter section introduces the concept of a multicriteria approach for estimating seed storage behaviour.
Download or read book Advances in Plant Breeding Strategies Breeding Biotechnology and Molecular Tools written by Jameel M. Al-Khayri and published by Springer. This book was released on 2016-02-02 with total page 656 pages. Available in PDF, EPUB and Kindle. Book excerpt: The basic concept of this book is to examine the use of innovative methods augmenting traditional plant breeding towards the development of new crop varieties under different environmental conditions to achieve sustainable food production. This book consists of two volumes: Volume 1 subtitled Breeding, Biotechnology and Molecular Tools and Volume 2 subtitled Agronomic, Abiotic and Biotic Stress Traits. This is Volume 1 which consists of 21 chapters covering domestication and germplasm utilization, conventional breeding techniques and the role of biotechnology. In addition to various biotechnological applications in plant breeding, it includes functional genomics, mutations and methods of detection, and molecular markers. In vitro techniques and their applications in plant breeding are discussed with an emphasis on embryo rescue, somatic cell hybridization and somaclonal variation. Other chapters cover haploid breeding, transgenics, cryogenics and bioinformatics.
Download or read book Cryopreservation of Plant Germplasm I written by Y. P. S. Bajaj and published by Springer Science & Business Media. This book was released on 2013-03-09 with total page 526 pages. Available in PDF, EPUB and Kindle. Book excerpt: The germ plasm of numerous plant species, especially those of forest trees, some agricultural crops, and medicinal plants, is endangered and threatened with extinction. This depletion of germplasm pools and the shrinkage of naturally occurring genetic resources have caused international concern. Conventionally, the germplasm of plants is conserved through seeds, tubers, roots, corms, rhizomes, bulbs, cuttings, etc. However, the germ plasm of a number of trees and plantation crops (such as coconut, cocao, coffee, oil palm, rubber, mango, horse chestnut, etc. ) cannot be preserved since their seed are short-lived (recalcitrant). Likewise, germplasm of vegetatively propagated crops (such as potato and cassava) cannot be stored on a long term basis and has to be grown and multiplied periodically in nurseries and fields. The plants are thus exposed to unpredictable weather conditions and diseases, with the result that instances are known where entire genetic stocks are lost. Therefore, unconventional methods are being developed for the storage and international exchange of germplasm. For this purpose in vitro cultures have been employed, but they can only enable short-to medium term preservation; moreover, cell cultures upon repeated subculture undergo genetic erosion. In view of the recent developments in the in vitro induction of genetic variability through somaclonal variation, somatic hybridization, recombinant DNA technology, etc. , new methods need to be employed for the storage of desirable cultures. In this regard freeze preservation of cells in liquid nitrogen (-196 0q, like that of semen, enables long-term storage, theoretically, for an indefinite period of time.
