Download or read book Pitfalls and Errors of HPLC in Pictures written by Veronika R. Meyer and published by Wiley-VCH. This book was released on 1999-01-06 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Errors are a common companion of all human acitvity, including work in the laboratory. Yet it is a great pity if erroneous results are produced with great effort and by using expensive instruments and demanding procedures. Therefore a book about sources of errors in high performance liquid chromatography, today′s most widely used analytical method, is highly recommended. Maybe the topic is not welcomed enthusiastically but, nevertheless, it is nessecary. In conception, at least, possibe problems can be devided into two categories. "Errors" are troublesome opponents of accurate and precise results which, however, can be understood. In contrast, "pitfalls" are totally unexpected intruders and the secret behind them is difficult to discover. The worst are those which are not detected but which affect the result anyway. The book does not, nevertheless, distinguish between the two types. The reader decide how they classify them. The single topics were presented in a clearly arranged style: a brief text with a corresponding figure which should encourage reading and thinking. Chemists and laboratory assistants get numerous hints how correct and reliable results could be transposed in daily laboratory work.

Download or read book Pitfalls and Errors of HPLC in Pictures written by Veronika Meyer and published by . This book was released on 1997 with total page 180 pages. Available in PDF, EPUB and Kindle. Book excerpt: Every user of HPLC is confronted with a multitude of pitfalls and sources of error. Adding 13 new topics to this new, second edition, Veronika Meyer now offers almost 100 exemplary solutions for such problems. All examples are presented with a concise, instructive text and an informative figure. These examples also include essential fundamentals as well as helpful strategies such as equipment tests or quality assurance strategies. A practice-oriented guide for obtaining correct and reliable analytical results! Book jacket.

Download or read book Pitfalls and Errors of HPLC in Pictures written by Veronika R. Meyer and published by John Wiley & Sons. This book was released on 2013-02-14 with total page 195 pages. Available in PDF, EPUB and Kindle. Book excerpt: The third edition of this popular problem-solving guide for this widely-used method includes eleven completely new examples and several updated ones, adding up to 100 contributions about pitfalls and errors in HPLC. Each example is presented on a double page with the text on the left-hand and a fi gure on the right-hand side, true to the motto 'a picture says more than a thousand words'. In addition, the author presents essential fundamentals as well as helpful strategies, such as equipment tests or quality assurance processes. New in this edition * Variability of the standard deviation * Infl uence of the acid type and concentration in the eluent * Water as an unintentional additive in the mobile phase * Inadequate purity of mobile phase water * Incomplete degassing * Inadequate stabilization of the extraction solvent * Tailing of phosphate compounds in the presence of steel * Different detection properties of diastereomers * Detector overload in ELSD * System suitability test * From repeatability to reproducibility A must-have resource for all users - showing how to use HPLC efficiently and obtain reliable results.

Download or read book Pitfalls and Errors of HPLC in Pictures written by Veronika Meyer and published by Wiley-VCH. This book was released on 2006-03-20 with total page 208 pages. Available in PDF, EPUB and Kindle. Book excerpt: The complexity of the HPLC method allows on one side a wide variety of usage. On the other side it is also a source of possible errors. This book does not only list them, but illustrate them with concrete examples. The topics were presented in a clearly arranged style: a brief text with a corresponding figure. Chemists and laboratory assistants get here numerous hints how correct and reliable results could be transposed in daily laboratory work.

Download or read book Chromatography written by James M. Miller and published by John Wiley & Sons. This book was released on 2005-12-16 with total page 518 pages. Available in PDF, EPUB and Kindle. Book excerpt: The first edition of Chromatography: Concepts and Contrasts, published in 1988, was one of the first books to discuss all the different types of chromatography under one cover. The second edition continues with these principles but has been updated to include new chapters on sampling and sample preparation, capillary electrophoresis and capillary electrochromatography (CEC), chromatography with mass spec detection, and industrial and governmental practices in regulated industries. Covers extraction, solid phase extraction (SPE), and solid phase microextraction (SPME), and introduces mass spectrometry Updated with the latest techniques in chromatography Discusses both liquid chromatography (LC)and gas chromatography(GC)

Download or read book HPLC Troubleshooting written by Günter J. Eppert and published by . This book was released on 2003 with total page 156 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Fundamentals of Environmental Sampling and Analysis written by Chunlong Zhang and published by John Wiley & Sons. This book was released on 2007-03-09 with total page 459 pages. Available in PDF, EPUB and Kindle. Book excerpt: An integrated approach to understanding the principles of sampling, chemical analysis, and instrumentation This unique reference focuses on the overall framework and why various methodologies are used in environmental sampling and analysis. An understanding of the underlying theories and principles empowers environmental professionals to select and adapt the proper sampling and analytical protocols for specific contaminants as well as for specific project applications. Covering both field sampling and laboratory analysis, Fundamentals of Environmental Sampling and Analysis includes: A review of the basic analytical and organic chemistry, statistics, hydrogeology, and environmental regulations relevant to sampling and analysis An overview of the fundamentals of environmental sampling design, sampling techniques, and quality assurance/quality control (QA/QC) essential to acquire quality environmental data A detailed discussion of: the theories of absorption spectroscopy for qualitative and quantitative environmental analysis; metal analysis using various atomic absorption and emission spectrometric methods; and the instrumental principles of common chromatographic and electrochemical methods An introduction to advanced analytical techniques, including various hyphenated mass spectrometries and nuclear magnetic resonance spectroscopy With real-life case studies that illustrate the principles plus problems and questions at the end of each chapter to solidify understanding, this is a practical, hands-on reference for practitioners and a great textbook for upper-level undergraduates and graduate students in environmental science and engineering.

Download or read book The HPLC Expert II written by Stavros Kromidas and published by John Wiley & Sons. This book was released on 2017-01-05 with total page 384 pages. Available in PDF, EPUB and Kindle. Book excerpt: How can I use my HPLC/UHPLC equipment in an optimal way, where are the limitations of the technique? These questions are discussed in detail in the sequel of the successful "HPLC Expert" in twelve chapters written by experts in the respective fields. The topics encompass - complementary to the first volume - typical HPLC users' problems and questions such as gradient optimization and hyphenated techniques (LC-MS). An important key aspect of the book is UHPLC: For which analytical problem is it essential, what should be considered? Besides presentation of latest developments directly from the main manufacturers, also UHPLC users and independent service engineers impart their knowledge. Consistent with the target groups, the level is advanced, but the emphasis is on practical applications.

Download or read book Method Validation in Pharmaceutical Analysis written by Joachim Ermer and published by John Wiley & Sons. This book was released on 2014-11-10 with total page 451 pages. Available in PDF, EPUB and Kindle. Book excerpt: This second edition of a global bestseller has been completely redesigned and extensively rewritten to take into account the new Quality by Design (QbD) and lifecycle concepts in pharmaceutical manufacturing. As in the first edition, the fundamental requirements for analytical method validation are covered, but the second edition describes how these are applied systematically throughout the entire analytical lifecycle. QbD principles require adoption of a systematic approach to development and validation that begin with predefined objectives. For analytical methods these predefined objectives are established as an Analytical Target Profile (ATP). The book chapters are aligned with recently introduced standards and guidelines for manufacturing processes validation and follow the three stages of the analytical lifecycle: Method Design, Method Performance Qualification, and Continued Method Performance Verification. Case studies and examples from the pharmaceutical industry illustrate the concepts and guidelines presented, and the standards and regulations from the US (FDA), European (EMA) and global (ICH) regulatory authorities are considered throughout. The undisputed gold standard in the field.

Download or read book Illustrated Pocket Dictionary of Chromatography written by Paul C. Sadek and published by John Wiley & Sons. This book was released on 2004-07-30 with total page 237 pages. Available in PDF, EPUB and Kindle. Book excerpt: Provides concise definitions, illustrations, formulas, and all of the other information that you need in a laboratory chromatography reference The Illustrated Pocket Dictionary of Chromatography offers the perfect quick reference to the parameters, systems, and components that support successful chromatographic separations. Concise, practical, and filled with detailed drawings, diagrams, and photographs, this pocket dictionary is designed for easy use in the laboratory. It provides a complete, up-to-date reference on analytical techniques such as gas chromatography, high-performance liquid chromatography, thin layer chromatography, and capillary electrophoresis as well as preparation techniques such as solid phase extraction and microextraction. The Illustrated Pocket Dictionary of Chromatography features: Explanations of key concepts Equations and formulas Parameters High-quality photographs of instruments, equipment, and components References for further research Explanations of common acronyms Ideal for the working chemist or technician in pharmaceutical, industrial, forensic, government, or other lab settings, the Illustrated Pocket Dictionary of Chromatography is also appropriate as a supplemental reference for analytical chemistry courses and labs. Students and analysts alike will find this compact yet comprehensive resource a valuable companion to their work in chromatography.

Download or read book Practical Instrumental Analysis written by Sergio Petrozzi and published by John Wiley & Sons. This book was released on 2012-09-24 with total page 388 pages. Available in PDF, EPUB and Kindle. Book excerpt: This practical book in instrumental analytics conveys an overview of important methods of analysis and enables the reader to realistically learn the (principally technology-independent) working techniques the analytical chemist uses to develop methods and conduct validation. What is to be conveyed to the student is the fact that analysts in their capacity as problem-solvers perform services for certain groups of customers, i.e., the solution to the problem should in any case be processed in such a way as to be "fit for purpose". The book presents sixteen experiments in analytical chemistry laboratory courses. They consist of the classical curriculum used at universities and universities of applied sciences with chromatographic procedures, atom spectrometric methods, sensors and special methods (e.g. field flow fractionation, flow injection analysis and N-determination according to Kjeldahl). The carefully chosen combination of theoretical description of the methods of analysis and the detailed instructions given are what characterizes this book. The instructions to the experiments are so detailed that the measurements can, for the most part, be taken without the help of additional literature. The book is complemented with tips for effective literature and database research on the topics of organization and the practical workflow of experiments in analytical laboratory, on the topic of the use of laboratory logs as well as on writing technical reports and grading them (Evaluation Guidelines for Laboratory Experiments). A small introduction to Quality Management, a brief glance at the history of analytical chemistry as well as a detailed appendix on the topic of safety in analytical laboratories and a short introduction to the new system of grading and marking chemicals using the "Globally Harmonized System of Classification and Labelling of Chemicals (GHS)", round off this book. This book is therefore an indispensable workbook for students, internship assistants and lecturers (in the area of chemistry, biotechnology, food technology and environmental technology) in the basic training program of analytics at universities and universities of applied sciences.

Download or read book Practical High Performance Liquid Chromatography written by Veronika R. Meyer and published by John Wiley & Sons. This book was released on 2013-03-25 with total page 342 pages. Available in PDF, EPUB and Kindle. Book excerpt: Jump into the HPLC adventure! Three decades on from publication of the 1st German edition of Veronika Meyer's book on HPLC, this classic text remains one of the few titles available on general HPLC aimed at practitioners. New sections on the following topics have been included in this fifth edition: Comparison of HPLC with capillary electrophoresis How to obtain peak capacity van Deemter curves and other coherences Hydrophilic interaction chromatography Method transfer Comprehensive two-dimensional HPLC Fast separations at 1000 bar HPLC with superheated water In addition, two chapters on the instrument test and troubleshooting in the appendix have been updated and expanded by Bruno E. Lendi, and many details have been improved and numerous references added. A completely new chapter is presented on quality assurance covering: Is it worth the effort? Verification with a second method Method validation Standard operating procedures Measurement uncertainty Qualifications, instrument test, and system suitability test The quest for quality Reviews of earlier editions "That this text is written by an expert in both the practice and teaching of HPLC is evident from the first paragraph....not only an enjoyable, fascinating and easy read, but a truly excellent text that has and will serve many teachers, students and practitioners very well." —The Analyst “...provides essential information on HPLC for LC practitioners in academia, industry, government, and research laboratories...a valuable introduction." - American Journal of Therapeutics

Download or read book Travelers Malaria written by Patricia Schlagenhauf-Lawlor and published by PMPH-USA. This book was released on 2008 with total page 428 pages. Available in PDF, EPUB and Kindle. Book excerpt: Travelers' Malaria is considered an essential resource for practitioners of travel medicine. This updated book focuses on the epidemiology, prevention and treatment of malaria in non-immune travelers and immigrants. Each chapter is an up-to-date monograph (with an abstract) and contains detailed references to published literature as well as to appropriate web sites. The purpose of the book is to serve as a reference for specialists in the field and for any practitioner who may confront the complexities of caring for malaria-exposed travelers in both pre- and post-travel settings.Travelers' Malaria contains 26 chapters.

Download or read book Introduction to Modern Liquid Chromatography written by Lloyd R. Snyder and published by John Wiley & Sons. This book was released on 2011-09-20 with total page 831 pages. Available in PDF, EPUB and Kindle. Book excerpt: The latest edition of the authoritative reference to HPLC High-performance liquid chromatography (HPLC) is today the leading technique for chemical analysis and related applications, with an ability to separate, analyze, and/or purify virtually any sample. Snyder and Kirkland's Introduction to Modern Liquid Chromatography has long represented the premier reference to HPLC. This Third Edition, with John Dolan as added coauthor, addresses important improvements in columns and equipment, as well as major advances in our understanding of HPLC separation, our ability to solve problems that were troublesome in the past, and the application of HPLC for new kinds of samples. This carefully considered Third Edition maintains the strengths of the previous edition while significantly modifying its organization in light of recent research and experience. The text begins by introducing the reader to HPLC, its use in relation to other modern separation techniques, and its history, then leads into such specific topics as: The basis of HPLC separation and the general effects of different experimental conditions Equipment and detection The column—the "heart" of the HPLC system Reversed-phase separation, normal-phase chromatography, gradient elution, two-dimensional separation, and other techniques Computer simulation, qualitative and quantitative analysis, and method validation and quality control The separation of large molecules, including both biological and synthetic polymers Chiral separations, preparative separations, and sample preparation Systematic development of HPLC separations—new to this edition Troubleshooting tricks, techniques, and case studies for both equipment and chromatograms Designed to fulfill the needs of the full range of HPLC users, from novices to experts, Introduction to Modern Liquid Chromatography, Third Edition offers the most up-to-date, comprehensive, and accessible survey of HPLC methods and applications available.

Download or read book HPLC Methods for Recently Approved Pharmaceuticals written by George Lunn and published by John Wiley & Sons. This book was released on 2005-05-06 with total page 743 pages. Available in PDF, EPUB and Kindle. Book excerpt: An indispensable resource for busy researchers Your time is valuable-too valuable to spend hunting through the technical literature in search of the right HPLC assay techniques for your projects. With HPLC Methods for Recently Approved Pharmaceuticals, you'll quickly identify and replicate the ideal procedures for your project needs, without having to refer to original source publications. More of your time can then be spent in the lab, not the library. Covering the relevant world literature through 2003, this book picks up where Dr. Lunn's acclaimed HPLC Methods for Pharmaceutical Analysis left off. It arms you with established HPLC assay techniques for hundreds of newly approved drugs, as well as drugs for which assay methods were only recently developed. Combining detailed descriptions of procedures with specially annotated references, this practical handbook gives you: * HPLC methods for 390 commonly prescribed pharmaceutical compounds * Various procedures for each drug listed together-making it easy to mix and match for customized approaches * Methods for drugs in biological fluids and for bulk and formulated drugs * Chemical structures, molecular weights and formulas, and CAS Registry Numbers * Cross-references to The Merck Index * Retention times of other drugs that can be assayed using the same methods

Download or read book Practical Methods in Cardiovascular Research written by Stefan Dhein and published by Springer. This book was released on 2006-02-28 with total page 1010 pages. Available in PDF, EPUB and Kindle. Book excerpt: Scientists working or planning to work in the field of cardiovascular research will welcome Methods in Cardiovascular Research as the reference book they have been waiting for. Not only general aspects of cardiovascular research are well presented but also detailed descriptions of methods, protocols and practical examples. Written by leading scientists in their field, chapters cover classical methods such as the Langendorff heart or working heart models as well as numerous new techniques and methods. Newcomers and experienced researchers alike will benefit from the troubleshooting guide in each chapter, the extensive reference lists for advanced reading and the great practical experience of the authors. Methods in Cardiovascular Research is a "must have" for anybody with an interest in cardiovascular research.

Download or read book Protein Purification written by Jan-Christer Janson and published by John Wiley & Sons. This book was released on 2012-01-03 with total page 542 pages. Available in PDF, EPUB and Kindle. Book excerpt: The authoritative guide on protein purification—now completely updated and revised Since the Second Edition of Protein Purification was published in 1998, the sequencing of the human genome and other developments in bioscience have dramatically changed the landscape of protein research. This new edition addresses these developments, featuring a wealth of new topics and several chapters rewritten from scratch. Leading experts in the field cover all major biochemical separation methods for proteins in use today, providing professionals in biochemistry, organic chemistry, and analytical chemistry with quick access to the latest techniques. Entirely new or thoroughly revised content includes: High-resolution reversed-phase liquid chromatography Electrophoresis in gels Conventional isoelectric focusing in gel slabs and capillaries and immobilized pH gradients Affinity ligands from chemical and biological combinatorial libraries Membrane separations Refolding of inclusion body proteins from E. coli Purification of PEGylated proteins High throughput screening techniques in protein purification The history of protein chromatography