Download or read book Phylogenetic Analysis of the Microbial Communities of Soils in the La Brea Asphalt Seeps and PAH Contaminated Rhizoplane written by Ian Nathaniel Balcom and published by . This book was released on 2007 with total page 296 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Dissertation Abstracts International written by and published by . This book was released on 2008 with total page 1006 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Temporal Changes in the Microbial Community of a PAH contaminated Soil During Bench top Bioremediation written by Caroline Gayle Rose and published by . This book was released on 2010 with total page 296 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Exploring Plant Rhizosphere Phyllosphere and Endosphere Microbial Communities to Improve the Management of Polluted Sites written by Michel Chalot and published by Frontiers Media SA. This book was released on 2022-01-11 with total page 419 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Long Term Impacts of a Genetically Engineered GEM and Polycyclic Aromatic Hydrocarbons PAHs on Soil Bacterial Communities written by Xiaoci Ji and published by . This book was released on 2014 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microbes capable of polyaromatic hydrocarbon (PAH) biodegradation can be used to remediate soils contaminated with these persistent pollutants. To monitor in situ PAH-biodegradation, the bioluminescent bio-reporter Pseudomonas fluorescens HK44, containing a lux luminescent gene cassette inserted into its naphthalene degradation operon, was released into PAH-contaminated soil in lysimeters in 1996. Three treatments were imposed: strain HK44 mixed with PAH-contaminated soil (PAH+, HK44+; n=3); strain HK44 mixed with uncontaminated soil (PAH–, HK44+; n=2) and PAH-contaminated soil alone (PAH+, HK44–; n=1). The objective of this study was to assess the long term impacts of these treatments on the indigenous soil bacterial community structure in the lysimeters. In 2010, 14 years after experiment initiation, replicate soil cores were taken from each lysimeter. Soil bacterial community structures were determined by 454 pyrosequencing of 16S rRNA gene amplicons. Even though PAH concentrations fell below detectable levels within the first couple years of the lysimeter experiment, PAH+ lysimeters showed significantly higher soil organic matter content (1.30 ± 0.23%) than PAH– lysimeters (0.81 ± 0.08%). 16S rRNA gene libraries reveal that there was a change in the bacterial community structure in PAH+ compared to PAH– lysimeters: 9.59% of OTUs (operational taxonomic units) were shared between PAH+ lysimeters while only 4.08% were shared between PAH+ and PAH– lysimeters. Multivariate ordination and cluster analysis, and phylogenetic tree-based analysis indicate that communities fell into three clusters: lysimeter 1 and 2 (both PAH+, HK44+); lysimeter 4 (PAH+, HK44+) and 6 (PAH+, HK44–); and lysimeter 3 and 5 (both PAH–, HK44+). Therefore over the long term, the addition of PAHs was more influential on bacterial community structure than the introduction of a GEM.
Download or read book Effect of Plant Host and Contamination on Structure of Rhizosphere Microbial Communities written by Olga A. Zelennikova and published by . This book was released on 2009 with total page 592 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Phylogenetic Physiological Profiling of Microbial Communities of Contaminated Soils written by and published by . This book was released on 2004 with total page 13 pages. Available in PDF, EPUB and Kindle. Book excerpt: The goals of this study were: (1) survey the microbial community in soil samples from a site contaminated with heavy metals using new rapid molecular techniques that are culture-independent; (2) identify phylogenetic signatures of microbial populations that correlate with metal ion contamination; and (3) cultivate these diagnostic strains using traditional as well as novel cultivation techniques in order to identify organisms that may be of value in site evaluation/management or bioremediation.
Download or read book Phylogenetic Characterization of Microbial Communities from Carbon Tetrachloride Contaminated Soil written by Cynthia K. Brinkman and published by . This book was released on 2006 with total page 74 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Microbial Community Structure During Bioremediation of Polycyclic Aromatic Hydrocarbon PAH Contaminated Soil written by Fengxiao Zhu and published by . This book was released on 2015 with total page 232 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Molecular Phylogenetic Analysis of Naturally Occurring Hyperthermophilic Microbial Communities written by Gene Stephen Wickham and published by . This book was released on 1995 with total page 410 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Rhizosphere Microbial Diversity in PAH s Contaminated and Uncontaminated Soil written by Livhuwani Priscilla Randima and published by . This book was released on 2009 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Molecular Based Methods Used for Studying Bacterial Diversity in Soils Contaminated with PAHs The Review written by Anna Gałązka and published by . This book was released on 2016 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Soil contamination could adversely affect microbial diversity, and perhaps also above- and below-ground ecosystem functioning. It is important to study microbial diversity not only for basic scientific research, but also to understand the link between diversity and community structure and function in the pollution site. The study of microbial diversity and their function in contaminated soil creates a serious problem because they observed significant limitations in methodology and taxonomy of this group. Methodology for the determination of bacterial diversity does not include their function in the soil and other environment areas. Microbes are known for their catabolic activity in bioremediation, but changes in microbial communities are still unpredictable. The bioremediation of a pollutant and its rate depend on the environmental conditions, number and type of the microorganisms, nature and chemical structure of the chemical compound being degraded. However, molecular methods have been used to study soil bacterial communities. While many anthropogenic activities, such as city development, agriculture, and use of pollution, can potentially affect soil microbial diversity, it is unknown how changes in microbial diversity can influence below-ground and above-ground ecosystems. There are problems associated with studying bacterial diversity in soil. These arise not only from methodological limitations, but also from a lack of taxonomic knowledge. Methods to measure microbial diversity in soil can be categorized into two groups: biochemical-based techniques and molecular-based techniques. But more common for studying microbial diversity in soil contaminated with polycyclic aromatic hydrocarbons are the molecular methods.
Download or read book Analysis of PAH degrading Bacteria Associated with Phytoremediation written by Endang Susilawati and published by . This book was released on 2003 with total page 170 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Microbial Taxonomy Phylogeny and Biodiversity written by Jesús L. Romalde and published by Frontiers Media SA. This book was released on 2019-12-31 with total page 375 pages. Available in PDF, EPUB and Kindle. Book excerpt: The great diversity of microbial life is the remaining major reservoir of unknown biological diversity on earth. To understand this vast, but largely unperceived diversity with its untapped genetic, enzymatic and industrial potential, microbial systematics is undergoing a revolutionary change in its approach to describe novel taxa based on genomic/envirogenomic information. The characterization of an organism is no longer bounded by methodological barriers, and it is now possible to fully sequence the whole genome of a strain to study individual genes, or to examine the genetic information by using different techniques. In fact, application of genomics is helping not only to provide a better understanding of the boundaries of genera and higher levels of classification, but also to refine our definition of the species concept. In addition, increased understanding of phylogeny is allowing to predict the genetic potential of microorganisms for biotechnological applications and adaptation to environmental changes. The present Research Topic on “Microbial Taxonomy, Phylogeny and Biodiversity” compiles a collection of papers covering the use of genomic sequence data in microbial taxonomy and systematics, including evolutionary relatedness of microorganisms; application of comparative genomics in systematic studies; or metagenomic approaches for biodiversity studies. We hope that this eBook incentives and encourages researchers for future discussions on microbial taxonomy and phylogenetics.
Download or read book Examination of the Bacterial Diversity of a PAH Contaminated Soil written by Jocelyn Lisa Fraga and published by . This book was released on 2001* with total page 140 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Molecular Analysis of Mycobacterium Community Shifts in Soil During the Biodegradation of Polycyclic Aromatic Hydrocarbons written by Pui Yi Cheung and published by . This book was released on 2001 with total page 278 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cing exogenous PAH-degraders, nutrient- and surfactant-amendments, and acclimation of soil microorganisms to PAHs, on PAH mineralization patterns and the degradative potential of the community were measured and correlated with Mycobacterium population dynamics. Results indicated that inoculation of soil with exogenous PAH-degrader had both positive and negative effects on PAH degradation, probably due to competition with indigenous microorganisms. Both nutrients and surfactants enhanced the rate and level of PAH degradation in contaminated soils, but the soil that had the highest level of mineralization had the least changes in Mycobacterium community structure. Pre-exposure of soil microorganisms to a PAH decreased the acclimation period and increased the initial rates for subsequent degradation of the same or a different PAH, in some soils. Contamination of soil samples with PAHs all resulted in Mycobacterium community shifts, as revealed by PCR-TGGE. The results obtained provided insight into understanding the ecological limitations to effective bioremediation of contaminated environmental sites.
Download or read book Successional Trajectories of Rhizosphere Bacterial Communities Over Consecutive Seasons written by and published by . This book was released on 2015 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: It is well known that rhizosphere microbiomes differ from those of surrounding soil, and yet we know little about how these root-associated microbial communities change through the growing season and between seasons. We analyzed the response of soil bacteria to roots of the common annual grass Avena fatua over two growing seasons using high-throughput sequencing of 16S rRNA genes. Over the two periods of growth, the rhizosphere bacterial communities followed consistent successional patterns as plants grew, although the starting communities were distinct. Succession in the rhizosphere was characterized by a significant decrease in both taxonomic and phylogenetic diversity relative to background soil communities, driven by reductions in both richness and evenness of the bacterial communities. Plant roots selectively stimulated the relative abundance of Alphaproteobacteria, Betaproteobacteria, and Bacteroidetes but reduced the abundance of Acidobacteria, Actinobacteria, and Firmicutes. Taxa that increased in relative abundance in the rhizosphere soil displayed phylogenetic clustering, suggesting some conservation and an evolutionary basis for the response of complex soil bacterial communities to the presence of plant roots. The reproducibility of rhizosphere succession and the apparent phylogenetic conservation of rhizosphere competence traits suggest adaptation of the indigenous bacterial community to this common grass over the many decades of its presence. We document the successional patterns of rhizosphere bacterial communities associated with a "wild" annual grass, Avena fatua, which is commonly a dominant plant in Mediterranean-type annual grasslands around the world; the plant was grown in its grassland soil. Most studies documenting rhizosphere microbiomes address "domesticated" plants growing in soils to which they are introduced. Rhizosphere bacterial communities exhibited a pattern of temporal succession that was consistent and repeatable over two growing seasons. There are few studies assessing the reproducibility over multiple seasons. Through the growing season, the rhizosphere community became progressively less diverse, likely reflecting root homogenization of soil microniches. Phylogenetic clustering of the rhizosphere dynamic taxa suggests evolutionary adaptation to Avena roots. The reproducibility of rhizosphere succession and the apparent phylogenetic conservation of rhizosphere competence traits suggest adaptation of the indigenous bacterial community to this common grass over the many decades of its presence.
