Download or read book Photochemical Probes in Biochemistry written by Peter E. Nielsen and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 304 pages. Available in PDF, EPUB and Kindle. Book excerpt: The concept of using photochemical probes in the study of biological systems was developed by Westheimer who published the first photoaffinity labeling experiments more than twenty years ago (J.Bio1. Chem. 237, (1962) 3006). Since then the concept has been used successfully in various areas of biochemistry and recently several new interesting and exciting aspects of the concept have been developed. It is the general opinion by scientists in the "field" that the full potential of photochemical probes in biochemical studies has far from been exploited yet. This is mostly due to the interdisciplinary character of the concept involving photochemistry, synthetic chemistry as well as biochemistry/molecular biology. The perspective of the NATO advanced workshop on "Photochemical Probes in Biochemistry", held in Holte (Copenhagen) Denmark 14-19, August, 1988, was several fold. The workshop was to give an account of the "state of the art" of using photochemical probes in biochemistry as well as to bring together specialists in photochemistry, syn thetic chemistry and molecular biology in order to analyze advantages as well as the inherent problems and pitfalls of the concept and provide suggestions and guidelines for fu ture research. Furthermore, it is the hope of the editor that the present publication which gives an account of the lectures presented at the workshop, will provide an introduction to scientists who are not familiar with photochemical probes, but to whom these could help answer central and pertinent questions.
Download or read book Photochemical Probes in Biochemistry written by Peter E Nielsen and published by . This book was released on 1989-04-30 with total page 324 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Chemical Probes in Biology written by Manfred P. Schneider and published by Springer Science & Business Media. This book was released on 2003 with total page 430 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Fluorescent Probes in Cellular and Molecular Biology written by Jan Slavik and published by CRC Press. This book was released on 1994-02-28 with total page 320 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescent Probes in Cellular and Molecular Biology presents the most comprehensive evaluation of the theory, instrumentation, and practical applications of fluorescent probes available. It covers basic principles and discusses the use of fluorescent dyes as probes for solvent polarity, solvent viscosity, membrane fluidity, membrane potential, and ionic intracellular composition. This important also describes various applications of fluorescent labels and photolabels, fluorescent analogs, and fluorogenic substrates. No similar publication is devoted exclusively to fluorescent probes, making this an essential guide for anyone working with modern fluorescence techniques. The book is perfect for individuals working in the areas of photochemistry, photobiology, biology, biophysics, or biochemistry. It is also an excellent textbook for graduate students in biology, biophysics, and medicine.
Download or read book Analytical Use of Fluorescent Probes in Oncology written by Elli Kohen and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 442 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence is a very powerful tool for work at the frontier of cell biology, photobiology and bioinstrumentation. The stated aim of the workshop was to highlight the significance of fluorescence work for the understanding of cell and tissue physiology, physiopathology and pharmacology, particulary in terms of the analytical use of fluorescent probes in oncology. In the organization of the workshop a multidisciplinary approach was selected. The purpose of the Advanced Research Workshop (ARW) was to bring together researchers in the various disciplines of tissue optics, imaging, microspectrofluorometry and state of the art probes, in order to explore the full benefits that can be derived in biomedicine through the convergence of these approaches. When applied to in vivo and in situ studies, fluorescence and related optical methods enable us to explore within tissues, cells and organelles photon effects previously understood only in solution photochemistry. Processes which can be studied at the molecular level by photophysics, photochemistry and physical chemistry can be evaluated in living tissue by fluorescence spectroscopy and imaging at the intracellular level in terms of structure and function. Thus, fluorescence adds a new dimension to cell biology and physiology. This approach is now supported by a full and versatile, rapidly growing armamentarium of new selective probes for organelles, enzymes, cations, cytoskeleton and metabolic control.
Download or read book Advances in Photochemistry written by Douglas C. Neckers and published by John Wiley & Sons. This book was released on 2009-09-24 with total page 252 pages. Available in PDF, EPUB and Kindle. Book excerpt: More than a simple survey of the current literature, Advances in Photochemistry offers critical evaluations written by internationally recognized experts. These pioneering scientists offer unique and varied points of view of the existing data. Their articles are challenging as well as provocative and are intended to stimulate discussion, promote further research, and encourage new developments in the field. This series provides photochemists a forum for critical, authoritative evaluations of advances in every area of photochemistry, including organic, inorganic, and biological topics. Volume 26 continues to report recent advances with a significant, up-to-date selection of papers by internationally recognized researchers. Topics include: Photochemistry of triarylmethane dye leuconitrites. Structure and reactivity of organic intermediates as revealed by time-resolved infrared spectroscopy. Semiconductor photocatalysis for organic synthesis. Photophysical probes of DNA sequence-directed structure and dynamics
Download or read book Less Common Metals in Proteins and Nucleic Acid Probes written by Michael J. Clarke and published by Springer. This book was released on 2014-03-12 with total page 233 pages. Available in PDF, EPUB and Kindle. Book excerpt: Some of the more interesting elements in the chemistry of life are less commonly occuring ones such as nickel and molybdenum. This volume elucidates the chemistry of these elements in important enzymes and also explores the chemistry of elements that do not normally occur in biological molecules, but are useful in probing their structure and function. Topics include: Acquisition and transport of Ni. Mechanistic action of Ni in a wide variety of enzymes. Multielectron redox systems involving pterins in proteins. Chemistry of the pterin and flavin complexes of Mo, Fe, Cu and Ru ions. Replacement of iron in transferrin by a number of other metal ions. Use of polypyridyl complexes of ruthenium and other transition metals as probes of nucleic acid structure through photochemical reactions.
Download or read book Fluorescent Probes written by Godfrey S. Beddard and published by . This book was released on 1981 with total page 256 pages. Available in PDF, EPUB and Kindle. Book excerpt: The photophysical and photochemical properties of probes in biological and in model systems are topics of intense current research. The wavelength, intensity, time and polarisation dependence of fluorescence probes can provide information on properties as diverse as electric fields and changes in conformation and may be used in the formidable task of establishing structure-function relationships in proteins and cell membranes. The wide scope of interactions which can be studied by these fluorescent molecules is illustrated in the articles in this volume which are based on talks, although much extended and updated, given at a meeting on 'Fluorescence Probes in Proteins and Membranes' held in the Royal Institution in November 1979.
Download or read book Fluorescent Energy Transfer Nucleic Acid Probes written by Vladimir V. Didenko and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 368 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescent nucleic acid probes, which use energy transfer, include such constructs as molecular beacons, molecular break lights, Scorpion primers, TaqMan probes, and others. These probes signal detection of their targets by changing either the intensity or the color of their fluorescence. Not surpr- ingly, these luminous, multicolored probes carry more flashy names than their counterparts in the other fields of molecular biology. In recent years, fluor- cent probes and assays, which make use of energy transfer, have multiplied at a high rate and have found numerous applications. However, in spite of this explosive growth in the field, there are no manuals summarizing different p- tocols and fluorescent probe designs. In view of this, the main objective of Fluorescent Energy Transfer Nucleic Acid Probes: Designs and Protocols is to provide such a collection. Oligonucleotides with one or several chromophore tags can form fluor- cent probes capable of energy transfer. Energy transport within the probe can occur via the resonance energy transfer mechanism, also called Förster tra- fer, or by non-Förster transfer mechanisms. Although the probes using Förster transfer were developed and used first, the later non-Förster-based probes, such as molecular beacons, now represent an attractive and widely used option. The term “fluorescent energy transfer probes” in the title of this book covers both Förster-based fluorescence resonance energy transfer (FRET) probes and probes using non-FRET mechanisms. Energy transfer probes serve as molecule-size sensors, changing their fluorescence upon detection of various DNA reactions.
Download or read book The Discovery and Utility of Chemical Probes in Target Discovery written by Paul Brennan and published by Royal Society of Chemistry. This book was released on 2020-11-17 with total page 343 pages. Available in PDF, EPUB and Kindle. Book excerpt: Numerous genetic methods can be utilised to link a phenotype to a single molecular target but annotated small molecule chemical probes and even entire chemogenomic libraries are increasingly being used as a complementary approach. This book will comprehensively cover the state of the art in chemical probes and best practice for use in target discovery, illustrated throughout with examples. Ideal for students and established biochemists, the book will also cover new technologies for probe discovery, new probe modalities, the new field of probes for RNA targets and the mature field of kinase chemical probes.
Download or read book Nucleic Acid Photophysics and Photochemistry written by Spiridoula Matsika and published by Springer. This book was released on 2024-11-01 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume focuses on recent advancements in our current understanding of nucleic acid photochemistry and its relationship to biologically relevant phenomena. Understanding the photophysical properties of nucleic acids is an area of longstanding and active research. Over the years, the field has greatly benefitted from steady advances in spectroscopic techniques and computational methods to study molecular excited states, which have facilitated detailed studies of the behavior of nucleic acids and their components after they absorb light. Experiments performed on ultrafast time scales (femtoseconds - picoseconds) have permitted the accurate determination of excited state lifetimes, while computational studies have provided detailed microscopic information about the mechanisms involved. As our understanding of the fundamental photophysics of nucleic acids has advanced, current studies now focus on systems of higher complexity, and introduce novel optical techniques to investigate the interactions between nucleic acids and proteins. Spectroscopic studies of nucleic acids, particularly nucleic acid constructs labeled with optical probes, can yield richly detailed information important to molecular biology, biochemistry and biophysics. This book is a must-read for anyone interested in the photophysical properties of nucleic acids and their role in biologically relevant phenomena.
Download or read book Photochemical and Photobiological Reviews written by Kendric Smith and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 309 pages. Available in PDF, EPUB and Kindle. Book excerpt: The goals of the science of photobiology can be divided into four categories: to develop (I) ways to optimize the beneficial effects of light on man and his environment, (2) methods to protect organisms, including man, from the detrimental effects of light, (3) photochemical tools for use in studies of life processes, and (4) photochemical therapies in medicine. To achieve these goals will require the knowledgeable collaboration of biologists, chemists, engineers, mathematicians, physicians, and physicists; because photobiology is a truly multidisciplinary science. While a multi disciplinary science is more intellectually demanding, it also has a greater potential for unexpected breakthroughs that can occur when data from several areas of science are integrated into new concepts for theoretical or practical use. Photochemical and Photobiological Reviews continues to provide in depth coverage of the many specialty areas of photobiology. It is hoped that these reviews will provide an important service to the younger scientists in the field and to senior scientists in related fields, because they provide a ready access to the recent literature in the field, and more importantly, they frequently offer a critical evaluation of the direction that the field is taking, or suggest a redirection when appropriate. Kendric C. Smith Editor vii Contents v Contents of Earlier Volumes ...................................... . Preface ............................................... . vii Chapter 1. The Activation of Enzymes with Light ................... .
Download or read book Photoacoustic Probes for In Vivo Imaging written by and published by Academic Press. This book was released on 2021-08-03 with total page 502 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters. Each chapter is written by an international board of authors Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Enzymology series Updated release includes the latest information on the Photoacoustic Probes for In Vivo Imaging
Download or read book Chiral Photochemical Scissors Targeting Proteins written by Challa Kumar and published by World Scientific. This book was released on 2018-10-23 with total page 447 pages. Available in PDF, EPUB and Kindle. Book excerpt: The remarkable ability to target one or even a couple of sites on a large protein with a small molecule, under photochemical control, is a considerable challenge and this challenge has been addressed in some depth in this book. Systematic modification of the structure of the photoreagent provided valuable information on the binding site recognition as well as the mechanism of the photocleavage reaction. Some factors that impact the photocleavage reaction include the exact location of the probe binding site on the protein, conformations of the bound probe at the binding site, protein size, functional groups present on the probe that interact with the protein surroundings either in a favorable or unfavorable manner, overall charge on the photoreagent, and photophysical as well as photochemical properties of the probe. The protein photocleavage studies, in all case, were preceded by detailed binding studies by a variety of spectroscopic methods. Methods as simple as absorption and fluorescence spectroscopies or more sophisticated circular dichroism spectroscopy were used. Conclusions that are most consistent with the binding data indicated a single binding site on most proteins, irrespective of the probe or the protein, with only one exception noted so far. Photoactivation of the bound probe resulted in protein cleavage at a single site, in many instances. The specificity for the reaction has been investigated in detail and molecular modeling studies provided a firm ground to rationalize the observed cleavage sites. The reagents provide unique tools for sequencing large proteins by converting them into smaller fragments by non-biochemical transformations. Understanding of the rules for the above methodology are also investigated which provided rational methods for the design of small molecules that could bind at particular sites on large proteins, and this is a major breakthrough for a variety of fields including drug design, protein targeting, mass spectrometry, proteomics and other cutting-edge research areas.
Download or read book Photochemistry and Photophysics written by Jan F. Rabek and published by CRC Press. This book was released on 1991-04-23 with total page 224 pages. Available in PDF, EPUB and Kindle. Book excerpt: Photochemistry and Photophysics is a multi-volume set that presents a critical review of new developments that have occurred in the inorganic, organic, atmospheric, environmental, material, bio-and polymer fields of photochemistry and photophysics over the last decade. Specific topics covered in Volume III include photochemical processes at semiconductors, photoluminescence probes of porous solids, photoluminescence probes of polymer structures, and photomodification of cell membranes. Topics covered in Volume IV include magnetic fields in photochemistry, heterogenous photocatalysis by semiconductor powders, hydrophobic and hydrophyllic effects on photochemical and photophysical processes, and photoinitiators for free radical polymerization. The book provides essential information for students and researchers in photochemistry and photophysics.
Download or read book Optical Probes in Biology written by Jin Zhang and published by CRC Press. This book was released on 2016-04-19 with total page 481 pages. Available in PDF, EPUB and Kindle. Book excerpt: Optical probes, particularly the fluorescent varieties, enable researchers to observe cellular events in real time and with great spatial resolution. Optical Probes in Biology explores the diverse capabilities of these powerful and versatile tools and presents various approaches used to design, develop, and implement them. The book examines the use
Download or read book Dynamic Studies in Biology written by Maurice Goeldner and published by John Wiley & Sons. This book was released on 2006-03-06 with total page 584 pages. Available in PDF, EPUB and Kindle. Book excerpt: With contributions by more than 30 expert researchers, this handbook covers the whole spectrum from chemistry to cell biology and from theory to application. In so doing, it deals with a broad range of topics from the chemistry and biophysics of caged compounds to their application in time-resolved studies, comparing the properties of different caging groups. The authors describe in detail light-activation of proteins as well as nucleic acids, while a special section is devoted to multiphoton phototriggers. A must-have for every biochemist, biophysicist and molecular biologist developing and working with these novel methods.
