Download or read book Partitioning In Aqueous Two Phase System written by Harry Walter and published by Elsevier. This book was released on 2012-12-02 with total page 731 pages. Available in PDF, EPUB and Kindle. Book excerpt: Partitioning in Aqueous Two-Phase Systems: Theory, Methods, Uses, and Applications to Biotechnology is a collection of papers that discusses the applications of aqueous two-phase systems to problems of separation and extraction of macromolecules, organelles, and cells. Papers focus on the theoretical basis and the practical details of the procedures used. Some of the papers describe in one or a few steps how two components can be separated by the investigator manipulating their partitions so that one component is in one phase and the other component is in the other phase or at the interface. Investigators can also avail of developed batch extractions for plant organelles, cell membranes, nucleic acids, and proteins. The book cites as an example the partitioning of right-side-out and inside-out vesicles (obtained from fragments of thylakoid membranes) to the top and bottom phases, respectively, of a Dx-PEG system. Other papers describe the use of the countercurrent distribution when single extraction steps are not sufficient to produce a separation in materials that do not differ greatly in their partitioning behavior. The collection can prove valuable for bio-chemists, cellular biologists, micro-biologists, and developmental biologists.
Download or read book Three Phase Partitioning written by Munishwar Nath Gupta and published by Elsevier. This book was released on 2021-08-11 with total page 346 pages. Available in PDF, EPUB and Kindle. Book excerpt: Three Phase Partitioning: Applications in Separation and Purification of Biological Molecules and Natural Products presents applications in diverse areas of both chemical technology and biotechnology. This book serves as a single resource for learning about both the economical, facile and scalable processes, along with their potential for applications in the separation and purification of materials and compounds across the entire spectra of chemical and biological nature. The book begins by explaining the origins and fundamentals of TPP and continues with chapters on related applications, ranging from the purification of parasite recombinant proteases to oil extraction from oilseeds and oleaginous microbes, and more. Written by researchers who have been pioneers in developing and utilizing three phase partitioning Focuses on applications, with chapters detailing relevance to a wide variety of areas and numerous practical examples Designed to give laboratory workers the information needed to undertake the challenge of designing successful three-phase partitioning protocols
Download or read book Chromatography written by and published by Krishna Prakashan Media. This book was released on with total page 412 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Aqueous Two Phase Partitioning written by Boris Y. Zaslavsky and published by CRC Press. This book was released on 1994-11-15 with total page 720 pages. Available in PDF, EPUB and Kindle. Book excerpt: Covers the fundamental principles of solute partitioning in aqueous two-phase systems, explains their important practical features, and furnishes methods of characterization. The information provided by the partition behaviour of a solute in an aqueous two-phase system is examined.
Download or read book Thermodynamics of Phase Equilibria in Food Engineering written by Camila Gambini Pereira and published by Academic Press. This book was released on 2018-10-17 with total page 682 pages. Available in PDF, EPUB and Kindle. Book excerpt: Thermodynamics of Phase Equilibria in Food Engineering is the definitive book on thermodynamics of equilibrium applied to food engineering. Food is a complex matrix consisting of different groups of compounds divided into macronutrients (lipids, carbohydrates, and proteins), and micronutrients (vitamins, minerals, and phytochemicals). The quality characteristics of food products associated with the sensorial, physical and microbiological attributes are directly related to the thermodynamic properties of specific compounds and complexes that are formed during processing or by the action of diverse interventions, such as the environment, biochemical reactions, and others. In addition, in obtaining bioactive substances using separation processes, the knowledge of phase equilibria of food systems is essential to provide an efficient separation, with a low cost in the process and high selectivity in the recovery of the desired component. This book combines theory and application of phase equilibria data of systems containing food compounds to help food engineers and researchers to solve complex problems found in food processing. It provides support to researchers from academia and industry to better understand the behavior of food materials in the face of processing effects, and to develop ways to improve the quality of the food products. Presents the fundamentals of phase equilibria in the food industry Describes both classic and advanced models, including cubic equations of state and activity coefficient Encompasses distillation, solid-liquid extraction, liquid-liquid extraction, adsorption, crystallization and supercritical fluid extraction Explores equilibrium in advanced systems, including colloidal, electrolyte and protein systems
Download or read book Laboratory Instrumentation written by Mary C. Haven and published by John Wiley & Sons. This book was released on 1994-10-28 with total page 522 pages. Available in PDF, EPUB and Kindle. Book excerpt: The new edition of this widely-used sourcebook details the startlingly array of diagnostic equipment available in the medical laboratory of the nineties, and also covers maintenance and quality assurance for each type of instrument. This book includes 17 completely rewritten chapters and 7 new ones, on nephelometry and turbidimetry, gas chromatography, mass spectrometry, flow cytometry, automated immunoassay systems, automated blood bank systems, and physician's office laboratory instrumentation.
Download or read book Handbook of Methods and Instrumentation in Separation Science written by and published by Academic Press. This book was released on 2009-11-11 with total page 881 pages. Available in PDF, EPUB and Kindle. Book excerpt: Handbook of Methods and Instrumentation in Separation Science, Volume 1 provides concise overviews and summaries of the main methods used for separation. It is based on the Encyclopedia of Separation Science. The handbook focuses on the principles of methods and instrumentation. It provides general concepts concerning the subject matter; it does not present specific procedures. This volume discusses the separation processes including affinity methods, analytical ultracentrifugation, centrifugation, chromatography, and use of decanter centrifuge and dye. Each methodology is defined and compared with other separation processes. It also provides specific techniques, principles, and theories concerning each process. Furthermore, the handbook presents the applications, benefits, and validation of the processes described in this book. This handbook is an excellent reference for biomedical researchers, environmental and production chemists, flavor and fragrance technologists, food and beverage technologists, academic and industrial librarians, and nuclear researchers. Students and novices will also find this handbook useful for practice and learning. One-stop source for information on separation methods General overviews for quick orientation Ease of use for finding results fast Expert coverage of major separation methods Coverage of techniques for all sizes of samples, pico-level to kilo-level
Download or read book Cell Separation written by Thomas G. Pretlow and published by Academic Press. This book was released on 2014-05-10 with total page 399 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell Separation: Methods and Selected Applications, Volume 4 provides information pertinent to the design and application of methods for the separation of cells. This book covers a variety of topics, including liver cells, epidermal Langerhans cells, isolation of oval cells, clonal analysis, and the purification of polymorphonuclear leukocytes. Organized into 17 chapters, this volume begins with an overview of the central role of the liver in the metabolism of the body. This text then provides the analysis of Langerhans cells that allow modulation of their function and provide approaches to the treatment of skin disease. Other chapters consider the biological significance of oval cells. This book discusses as well the elucidation of the mechanisms of cellular proliferation, function, and differentiation in living tissues. The final chapter deals with the important applications of cell culture that involve continuous cell lines. This book is a valuable resource for cell biologists, experimental oncologists, hematologists, immunologists, and endocrinologists.
Download or read book Stationary Phases in Gas Chromatography written by G. E. Baiulescu and published by Elsevier. This book was released on 2013-10-22 with total page 377 pages. Available in PDF, EPUB and Kindle. Book excerpt: Stationary Phases in Gas Chromatography is a four-chapter book that begins with a description of chromatographic column, including its retention parameters; mobile and stationary phases; optimization of its process; and types of column in gas chromatography. Subsequent chapter focuses on the retention data and thermodynamic variables in the technique. The last two chapters report the different types of stationary phases in gas chromatography, as well as their selectivity.
Download or read book Chromatography for the Analysis of Lipids written by Eugene W. Hammond and published by CRC Press. This book was released on 1993-02-09 with total page 206 pages. Available in PDF, EPUB and Kindle. Book excerpt: Chromatography for the Analysis of Lipids focuses on the three main chromatographic techniques for analyzing lipids: thin layer (TLC), gas liquid (GLC), and high performance liquid chromatography (HPLC). It also covers supercritical fluid chromatography (SFC). Applications for each chromatographic technique are discussed with emphasis on their quantitative aspects and suitability for use, and variations in technique are covered in detail. Figures are used throughout the book to illustrate typical results, which shows end points that can be compared in your own laboratory. This important technical book will be an excellent workbench companion for chromatographers, food scientists, lipid chemists, analytical chemists, and others who use chromatographic techniques for lipid analysis.
Download or read book Advances in Intelligent Data Analysis written by David Hand and published by Springer Science & Business Media. This book was released on 1999-07-28 with total page 529 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book constitutes the refereed proceedings of the Third International Symposium on Intelligent Data Analysis, IDA-99 held in Amsterdam, The Netherlands in August 1999. The 21 revised full papers and 23 posters presented in the book were carefully reviewed and selected from a total of more than 100 submissions. The papers address all current aspects of intelligent data analysis; they are organized in sections on learning, visualization, classification and clustering, integration, applications and media mining.
Download or read book Nuclear Science Abstracts written by and published by . This book was released on 1967-11 with total page 874 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Determination of Octanol water Distribution Coefficients Water Solubilities and Sediment water Partition Coefficients for Hydrophobic Organic Pollutants written by Samuel W. Karickhoff and published by . This book was released on 1979 with total page 28 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Conservation of Easel Paintings written by Joyce Hill Stoner and published by Routledge. This book was released on 2013-02-15 with total page 916 pages. Available in PDF, EPUB and Kindle. Book excerpt: Conservation of Easel Paintings is the first comprehensive text on the history, philosophy, and methods of treatment of easel paintings that combines both theory with practice. With contributions from an international group of experts and interviews with important artists, this volume provides an all-encompassing guide to necessary background knowledge in technical art history, artists' materials, scientific methods of examination and documentation, with sections that present varying approaches and methods for treatment, including consolidation, lining, cleaning, retouching, and varnishing. The book concludes with a section featuring issues of preventive conservation, storage, shipping, exhibition, lighting, safety issues, and public outreach. Conservation of Easel Paintings is a crucial resource in the training of conservation students and will provide generations of practicing paintings conservators and interested art historians, curators, directors, collectors, dealers, artists, and students of art and art history with invaluable information and guidance.
Download or read book Chemical Engineering Volume 2 written by J H Harker and published by Elsevier. This book was released on 2013-10-22 with total page 1219 pages. Available in PDF, EPUB and Kindle. Book excerpt: Chemical Engineering Volume 2 covers the properties of particulate systems, including the character of individual particles and their behaviour in fluids. Sedimentation of particles, both singly and at high concentrations, flow in packed and fluidised beads and filtration are then examined. The latter part of the book deals with separation processes, such as distillation and gas absorption, which illustrate applications of the fundamental principles of mass transfer introduced in Chemical Engineering Volume 1. In conclusion, several techniques of growing importance - adsorption, ion exchange, chromatographic and membrane separations, and process intensification - are described. A logical progression of chemical engineering concepts, volume 2 builds on fundamental principles contained in Chemical Engineering volume 1 and these volumes are fully cross-referenced Reflects the growth in complexity and stature of chemical engineering over the last few years Supported with further reading at the end of each chapter and graded problems at the end of the book
Download or read book The Biology of Cholesterol and Related Steroids written by N. B. Myant and published by Butterworth-Heinemann. This book was released on 2014-04-24 with total page 925 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Biology of Cholesterol and Related Steroids focuses on the study of sterols in relation to living organisms. The publication first takes a look at the analysis of sterols and related steroids and the distribution of sterols and related steroids in nature, as well as the processes of extraction and separation and presence of sterols in plants, fungi, vertebrates, and invertebrates. The text then ponders on biosynthesis of sterols and metabolism of cholesterol. Topics include formation of fatty acid esters of cholesterol, steroid hormones, biosynthetic pathway to sterols, reaction mechanisms, and comparative aspects of sterol synthesis. The manuscript examines the developmental aspects of cholesterol metabolism and sterols in biological membranes. The book also reviews cholesterol synthesis in animal tissues, sterol metabolism in isolated cells, and epidemiology of the plasma cholesterol. Discussions focus on selection of statistical populations, genetic influences, regulation of sterol synthesis, general aspects of sterol metabolism, and removal of cell cholesterol in vivo. The publication is a dependable source of data for biochemists and readers interested in the biology of cholesterol and steroids.
Download or read book Aqueous Two Phase Systems written by Rajni Hatti-Kaul and published by Springer Science & Business Media. This book was released on 2008-02-05 with total page 442 pages. Available in PDF, EPUB and Kindle. Book excerpt: A mixture of two polymers, or one polymer and a salt, in an aqueous medium separates into two phases: this phenomenon is useful in biotechn- ogy for product separations. Separation of biological molecules and particles in these aqueous two-phase systems (ATPS) was initiated over 40 years ago by P.-Å. Albertsson, and later proved to be of immense utility in biochemical and cell biological research. A boost in the application of ATPS was seen when problems of separations in biotechnology processes were encountered. Its simplicity, biocompatibility, and amenability to easy scaleup operations make the use of ATPS very attractive for large-scale bioseparations. Despite the advantages ATPS enjoys over other separation techniques, the application of two-phase systems has for a long time been confined to selected labora- ries. Recent years have, however, shown a trend in which increasing numbers of researchers employ two-phase partitioning techniques in both basic and applied research.
