Download or read book Peptide Lipid Interactions written by Sidney A. Simon and published by Academic Press. This book was released on 2002-11-13 with total page 606 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume contains a comprehensive overview of peptide-lipid interactions by leading researchers. The first part covers theoretical concepts, experimental considerations, and thermodynamics. The second part presents new results obtained through site-directed EPR, electron microscopy, NMR, isothermal calorimetry, and fluorescence quenching. The final part covers problems of biological interest, including signal transduction, membrane transport, fusion, and adhesion. Key Features * world-renowned experts * state-of-the-art experimental methods * monolayers, bilayers, biological membranes * theoretical aspects and computer simulations * rafts * synaptic transmission * membrane fusion * signal transduction
Download or read book Protein Lipid Interactions written by C.Reyes Mateo and published by Springer Science & Business Media. This book was released on 2008-09-30 with total page 260 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biological membranes have long been identified as key elements in a wide variety of cellular processes including cell defense communication, photosynthesis, signal transduction, and motility; thus they emerge as primary targets in both basic and applied research. This book brings together in a single volume the most recent views of experts in the area of protein–lipid interactions, providing an overview of the advances that have been achieved in the field in recent years, from very basic aspects to specialized technological applications. Topics include the application of X-ray and neutron diffraction, infrared and fluorescence spectroscopy, and high-resolution NMR to the understanding of the specific interactions between lipids and proteins within biological membranes, their structural relationships, and the implications for the biological functions that they mediate. Also covered in this volume are the insertion of proteins and peptides into the membrane and the concomitant formation of definite lipid domains within the membrane.
Download or read book Protein Lipid Interactions written by Lukas K. Tamm and published by John Wiley & Sons. This book was released on 2006-05-12 with total page 470 pages. Available in PDF, EPUB and Kindle. Book excerpt: In 17 contributions by leading research groups, this first comprehensive handbook in the field covers the interactions between proteins and lipids that make the fabric of biological membranes from every angle. It examines the relevant hermodynamic and structural issues from a basic science perspective, and goes on to discuss biochemical and cell biological processes. The book covers physical principles as well as mechanisms of membrane fusion and fission. Additionally, chapters on bilayer structure and protein-lipid interactions as well as on how proteins shape lipids and vice versa, membrane penetration by toxins, protein sorting, and allosteric regulation of signal transduction across membranes make this a valuable information source for researchers in academia and industry.
Download or read book Membrane Protein Lipid Interactions Physics and Chemistry in the Bilayer written by Jordi H. Borrell and published by Springer. This book was released on 2016-03-15 with total page 116 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book has been conceives as a brief introduction to biomembranes physical chemistry for undergraduate students of sciences, and it is particularly dedicated to the lipid-protein membrane interactions. A general introduction is presented in Chapters 1 and 2. The following Chapters, 3 and 4, describe the most accepted theories on lipid-membrane protein interactions as well as the new experimental approaches, in particular, these arose from nano sciences as atomic for microscopy and single molecule force spectroscopy. The book emphasizes the relevance of physical parameters as the lateral surface pressure and the lipid curvature as actors for understanding the physicochemical properties of the biomembranes.
Download or read book Protein Lipid Interactions written by Anthony Watts and published by Elsevier. This book was released on 1993-10-08 with total page 378 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protein-lipid interactions as a field of study is now a mature area, and this volume of New Comprehensive Biochemistry has been published with two objectives in mind. Firstly, to look to the future, and try to envisage how the subject may develop in the near to medium future. Secondly, to present contrasting or complementary views on the same system. For example, the acetylcholine receptor is discussed from a predominantly structural aspect by Barrantes, and from the kinetic standpoint by Rankin, et al. The volume not only gives an update on specific aspects of the field, but also shows the way in which the phenomenon of protein-lipid interactions is now seemingly infiltrating many areas of biomembrane research, from recombinant DNA studies, protein insertion and assembly and reconstitution considerations to structural studies of membrane proteins.
Download or read book Free Energy Calculations written by Christophe Chipot and published by Springer Science & Business Media. This book was released on 2007-01-08 with total page 528 pages. Available in PDF, EPUB and Kindle. Book excerpt: Free energy constitutes the most important thermodynamic quantity to understand how chemical species recognize each other, associate or react. Examples of problems in which knowledge of the underlying free energy behaviour is required, include conformational equilibria and molecular association, partitioning between immiscible liquids, receptor-drug interaction, protein-protein and protein-DNA association, and protein stability. This volume sets out to present a coherent and comprehensive account of the concepts that underlie different approaches devised for the determination of free energies. The reader will gain the necessary insight into the theoretical and computational foundations of the subject and will be presented with relevant applications from molecular-level modelling and simulations of chemical and biological systems. Both formally accurate and approximate methods are covered using both classical and quantum mechanical descriptions. A central theme of the book is that the wide variety of free energy calculation techniques available today can be understood as different implementations of a few basic principles. The book is aimed at a broad readership of graduate students and researchers having a background in chemistry, physics, engineering and physical biology.
Download or read book Current Topics in Membranes written by Sidney A. Simon and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: "This volume contains a comprehensive overview of peptide-lipid interactions by leading researchers. The first part covers theoretical concepts, experimental considerations, and thermodynamics. The second part presents new results obtained through site-directed EPR, electron microscopy, NMR, isothermal calorimetry, and fluorescence quenching. The final part covers problems of biological interest, including signal transduction, membrane transport, fusion, and adhesion."--[Source inconnue].
Download or read book Progress in Protein lipid Interactions written by A. Watts and published by Elsevier Science & Technology. This book was released on 1985 with total page 316 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Membrane Peptide Interactions written by Nuno C. Santos and published by . This book was released on 2020 with total page 302 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book summarizes the importance of peptide-membrane interactions, mostly aiming at developing new therapeutic approaches. The experimental and computational methodologies used to investigate such interactions reveal the evolution of existing biophysical methodologies, shedding some light on potential applications of peptides, as well as on the improvement of their design. Understanding the determinants for peptide-membrane interactions may also improve the knowledge of membrane functions such as the membrane transport, fusion, and signaling processes, contributing to the development of new agents for highly relevant applications ranging from disease treatment to food technology.
Download or read book Peptide Liposome Model Systems for Triggered Release written by Camilla Skyttner and published by Linköping University Electronic Press. This book was released on 2018-09-12 with total page 78 pages. Available in PDF, EPUB and Kindle. Book excerpt: Liposomes are widely used in drug delivery to improve drug efficacy and to reduce side effects. For liposome-encapsulated drugs to become bioavailable and provide a therapeutic effect they must be released, which typically is a slow process that primarily relies on passive diffusion, liposome rupture or endocytotic uptake. Achieving drug concentrations within the therapeutic window can thus be challenging, resulting in poor efficacy and higher risks drug resistance. Finding means to modulate lipid membrane integrity and to trigger rapid and efficient release of liposomal cargo is thus critical to improve current and future liposomal drug delivery systems. The possibilities to tailor lipid composition and surface functionalization is vital for drug delivery applications but also make liposomes attractive model systems for studies of membrane active biomolecules. The overall aim of this thesis work has been to develop new strategies for triggering and controlling changes in lipid membrane integrity and to study the interactions of membrane active peptides with model lipid membranes using both de novo designed and biologically derived synthetic amphipathic cationic peptides. Two different sets of designed peptides have been explored that can fold and heterodimerize into a coiled coil and helix-loop-helix fourhelix bundle, respectively. Conjugation of the cationic lysine rich peptides to liposomes triggered a rapid and concentration dependent release. The additions of their corresponding glutamic acid-rich complementary peptides inhibited the release of liposomal cargo. Possibilities to reduce the inhibitory effect by both proteolytic digestion of the inhibitory peptide and by means of heterodimer exchange have been investigated. Moreover, the effects of peptide size and composition and ability to fold have been studied in order to elucidate the factors that influence the membrane permeabilizing effects of the peptides. In addition, the membrane activity of a the two-peptide bacteriocin PLNC8? and PLNC8? has been explored using liposomes as a model system. PLNC8?? are expressed by Lactobacillus plantarum and were shown to display pronounced membrane-partition folding coupling, leading to rapid release of liposome encapsulated carboxyfluorescein. PLNC8?? also kill and suppressed growth of the gram-negative bacteria Porphyromonas gingivalis by efficiently damaging the bacterial membrane. Although membrane active peptides are highly efficient in perturbing lipid membrane integrity, possibilities to trigger release using external stimuli are also of large interest for therapeutic applications. Light-induced heating of liposome encapsulated gold nanoparticles (AuNPs) has been shown by others as a potential strategy to trigger drug release. To facilitate fabrication of thermoplasmonic liposome systems we developed a simple method for synthesis of small AuNPs inside liposomes, using the liposomes as nanoscale reaction vessels. The work presented in this thesis provides new knowledge and techniques for future development of liposome-based drug delivery systems, peptide-based therapeutics and increase our understanding of peptide-lipid interactions.
Download or read book Lipid Bilayers written by J. Katsaras and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 304 pages. Available in PDF, EPUB and Kindle. Book excerpt: Provides the reader with an up to date insight of the current state of the art in the field of lipid bilayer research and the important insights derived for the understanding of the complex and varied behaviour of biological membranes and its function.
Download or read book Membrane active Peptides written by Miguel A. R. B. Castanho and published by Internat'l University Line. This book was released on 2010 with total page 675 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Food Proteins and Peptides written by Navam S. Hettiarachchy and published by CRC Press. This book was released on 2012-03-19 with total page 473 pages. Available in PDF, EPUB and Kindle. Book excerpt: A multidisciplinary resource, Food Proteins and Peptides: Chemistry, Functionality, Interactions, and Commercialization enables researchers in biochemistry, biotechnology, food science and technology, nutrition, and medicine to understand the physicochemical and biochemical factors that govern the functionality of these food components. Following chapters on the structure and chemistry of amino acids, peptides, and proteins, the book describes modes of characterization and the functional relationships of food proteins. It examines protein solubility and insolubility and explores proteins and peptides as emulsifying and foaming agents. Specialized topics include: Factors affecting heat-induced casein–whey protein interactions in bovine milk systems The effects of protein–saccharide interactions on the properties of food components Ameliorative action of peptides on cholesterol and lipid metabolism Proteins and peptides with elements of sweetness, kokumi, umami, and bitterness A new approach for the large-scale fractionation of peptides based on their amphoteric nature The book examines the source of bioactive peptides and describes their bioavailability, including their absorption and occurrence in human blood. It also provides a database of biologically active proteins and peptides. Final chapters review current status, future industrial perspectives, and future trends of bioactive food proteins and peptides and explore the role of nanotechnology in protein research. With contributions from a panel of international scientists, this volume captures the state of the art in protein and peptide research, providing a launching pad for further inquiry and discovery.
Download or read book Membrane Dynamics and Domains written by Peter J. Quinn and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 502 pages. Available in PDF, EPUB and Kindle. Book excerpt: The fluid-mosaic model of membrane structure formulated by Singer and Nicolson in the early 1970s has proven to be a durable concept in terms of the principles governing the organization of the constituent lipids and proteins. During the past 30 or so years a great deal of information has accumulated on the composition of various cell membranes and how this is related to the dif ferent functions that membranes perform. Nevertheless, the task of explaining particular functions at the molecular level has been hampered by lack of struc tural detail at the atomic level. The reason for this is primarily the difficulty of crystallizing membrane proteins which require strategies that differ from those used to crystallize soluble proteins. The unique exception is bacteriorhodopsin of the purple membrane of Halobacterium halobium which is interpolated into a membrane that is neither fluid nor in a mosaic configuration. To date only 50 or so membrane proteins have been characterised to atomic resolution by diffraction methods, in contrast to the vast data accumulated on soluble proteins. Another factor that has been difficult to explain is the reason why the lipid compliment of membranes is often extremely complex. Many hundreds of different molecular species of lipid can be identified in some membranes. Remarkably, the particular composition of each membrane appears to be main tained within relatively narrow limits and its identity distinguished from other morphologically-distinct membranes.
Download or read book Inhibitors of Protein Protein Interactions written by Ali Tavassoli and published by Royal Society of Chemistry. This book was released on 2020-12-07 with total page 357 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protein-protein interactions (PPI) are at the heart of the majority of cellular processes, and are frequently dysregulated or usurped in disease. Given this central role, the inhibition of PPIs has been of significant interest as a means of treating a wide variety of diseases. However, there are inherent challenges in developing molecules capable of disrupting the relatively featureless and large interfacial areas involved. Despite this, there have been a number of successes in this field in recent years using both traditional drug discovery approaches and innovative, interdisciplinary strategies using novel chemical scaffolds. This book comprehensively covers the various aspects of PPI inhibition, encompassing small molecules, peptidomimetics, cyclic peptides, stapled peptides and macrocycles. Illustrated throughout with successful case studies, this book provides a holistic, cutting-edge view of the subject area and is ideal for chemical biologists and medicinal chemists interested in developing PPI inhibitors.
Download or read book Interaction of Antimicrobial Peptides with Model Lipid Membranes written by Ahmad Arouri and published by . This book was released on 2009 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The antimicrobial resistance is a growing problem that threatens our ability to treat infections and urges the need to develop novel alternatives for "conventional" antibiotics which are less susceptible for the development of bacterial resistance. Antimicrobial peptides (AMPs) are among the most promising candidates. This is due to their ability to disturb biological membranes via non-specific interactions with the membrane lipid components. In spite of the significant amount of experimental data, many of the issues concerning AMPs are still not well understood. This includes their molecular-based mechanism of action, the role of the membrane architecture, the importance of the structure and properties of the antimicrobial peptides, the extent and significance of the pore formation and membrane micellization processes, and others. We utilized several biophysical techniques, mainly DSC, ITC, DLS, IRRAS, FT-IR, and epifluorescence microscopy, to study the interaction of synthetic AMPs with various model lipid membranes of different composition. In this work two groups of AMPs were used: 1) KLA peptides with different helical propensities, and 2) a linear and a cyclic arginine- and tryptophan-rich antimicrobial hexapeptide with identical amino acid sequences (Ac-RW and C-RW, respectively). Phosphatidylglycerol (PG), phosphatidylethanolamine (PE) and PG/PE mixtures were used to model the cytoplasmic bacterial membrane, whereas phosphatidylcholine (PC) was used to model the outer leaflet of mammalian membranes. Our aim was to investigate the interplay between the peptide conformation, the membrane composition and properties, and the experimental conditions, as well as the role of membrane composition in modulating the activity of the peptides. In addition, an attempt was made to explain the bioactivity of these peptides in the light of the experimental results using model membranes. This study enriches our knowledge and understanding of the complicated interaction between AMP and model membranes. The coupling of various biophysical techniques is very beneficial to get insight into the studied systems from different perspectives as well as to check any variation in experimental results due to different techniques. Our findings emphasize the importance of the peptide conformation and local concentration in determining the mode of action of the peptides. Also, the findings show that a phenomenon observed with certain lipid membranes, preparation procedures, and experimental conditions cannot necessarily be generalized.
Download or read book Lipid Protein Interactions written by Jörg H. Kleinschmidt and published by Humana Press. This book was released on 2013-02-14 with total page 464 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biological membranes are the essential structuring elements of all living cells. Many enzymatic reactions take place at the membrane-water interface. To gain detailed insight into membrane properties, it is therefore of great importance to understand the complex nature of the interactions of membrane proteins with lipids. Lipid-Protein Interactions: Methods and Protocols provides a selection of protocols to examine protein-lipid interactions, membrane and membrane protein structure, how membrane proteins affect lipids and how they are in turn affected by the lipid bilayer and lipid properties. The methods described here are all actively used, complementary, and necessary to obtain comprehensive information about membrane structure and function. They include label-free approaches, imaging techniques and spectroscopic methodologies. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Lipid-Protein Interactions: Methods and Protocols seeks to serve both professional and novices with its wide range of the methods frequently used in this area of research.
