Download or read book Peptide Antibodies written by Gunnar Houen and published by Humana. This book was released on 2015-10-02 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This extensive volume covers basic and advanced aspects of peptide antibody production, characterization and uses. Although peptide antibodies have been available for many years, they continue to be a field of active research and method development. For example, peptide antibodies which are dependent on specific posttranslational modifications are of great interest, such as phosphorylation, citrullination and others, while different forms of recombinant peptide antibodies are gaining interest, notably nanobodies, single chain antibodies, TCR-like antibodies, among others. Within this volume, those areas are covered, as well as several technical and scientific advances: solid phase peptide synthesis, peptide carrier conjugation and immunization, genomics, transcriptomics, proteomics and elucidation of the molecular basis of antigen presentation and recognition by dendritic cells, macrophages, B cells and T cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Peptide Antibodies: Methods and Protocols serves as an ideal reference for researchers exploring this vital and expansive area of study.
Download or read book Peptide Antibodies written by Gunnar Houen and published by Springer Nature. This book was released on with total page 270 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Janeway s Immunobiology written by Kenneth Murphy and published by Garland Science. This book was released on 2010-06-22 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The Janeway's Immunobiology CD-ROM, Immunobiology Interactive, is included with each book, and can be purchased separately. It contains animations and videos with voiceover narration, as well as the figures from the text for presentation purposes.
Download or read book Peptide Antigens written by G. Brian Wisdom and published by Irl Press. This book was released on 1994 with total page 252 pages. Available in PDF, EPUB and Kindle. Book excerpt: Peptide antigens and anti-peptide antibodies are widely used in biochemistry and molecular biology for the measurement, location, and purification of specific oligopeptides. More recently the application of these reagents has expanded, for example in the identification and mapping of the binding sites of antibodies and T-cell receptors and in the identification and characterization of proteins which are known only by their primary structure. This volume provides practical guidance to the major techniques used in the exploitation of peptide antigens and anti-peptide antibodies. The chapters give detailed protocols for the prediction of epitopes, peptides synthesis, the preparation of peptide immunogens, immunoaffinity chromatography, immunoassays, and the mapping of epitopes using both synthetic peptides and phage display systems. Peptide Antigens: A Practical Approach covers all practical aspects of this important and growing subject. It is a unique compendium of methods for workers in biochemistry, molecular biology, and immunology who need to use this technology in their research.
Download or read book Design Production and Characterization of Peptide Antibodies written by Nicole Hartwig Trier and published by Mdpi AG. This book was released on 2023-07-05 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Antibodies are key reagents in diagnostics and experimental biology, capable of detecting numerous antigenic targets. Proteins are often in focus and are usually effective targets for antibody production. Occasionally, however, the native protein is known but not available, or a very specific target is required. In these cases, synthetic peptides, copying essential sequences from the target, are good alternatives for antibody production. Peptide antibodies have been used in diagnostics and experimental biology with great success, especially because they can be produced to multiple targets, for example, native and denatured targets. Traditional peptide antibody proteins encompass animal-based immunization with a synthetic peptide, usually conjugated to a carrier protein to enhance immune presentation, as small peptides tend not to be immunogenic by themselves. There are several strategies for the conjugation of peptides to carriers applied for immunization. This is the most used approach for peptide antibody production. In addition to traditional peptide antibody production, peptide antibodies can be produced using libraries or sequencing. This Special Issue, "Design, Production and Characterization of Peptide Antibodies", aims to describe the current state-of-the-art techniques and characterization/applications within the field as well as new and emerging uses of peptide antibodies.
Download or read book Monoclonal Antibodies and Peptide Therapy in Autoimmune Diseases written by Jean-François Bach and published by Marcel Dekker. This book was released on 1993 with total page 424 pages. Available in PDF, EPUB and Kindle. Book excerpt: Combining principles of modern immunology with applications to immunopathology, this reference documents developments in the immunotherapy of autoimmune diseases - emphasizing monoclonal antibody and peptide approaches. receptor itself to intervening at the level of the major histocompatibility complex (MHC) antigens, Monoclonal Antibodies and Peptide Therapy in Autoimmune Diseases: provides an update on T-cell basic science; discusses most monoclonal antibodies used in autoimmune disease experiments; describes the concept of peptide therapy in its various forms; and presents fundamental pharmacological data that clarify the clinical usage of these new agents. Therapy in Autoimmune Diseases should be a useful resource for immunologists, rheumatologists, pharmacologists, pathologists, dermatologists, nephrologists, and graduate and medical school students in these disciplines.
Download or read book Antigen Binding Molecules Antibodies and T Cell Receptors written by and published by Academic Press. This book was released on 1996-12-13 with total page 561 pages. Available in PDF, EPUB and Kindle. Book excerpt: Topics Covered Include: X-ray crystallography of ligands. Catalytic antibodies. Nature of the antigen. Antibody binding sites. Maturation of the immune response. Computational biochemistry of antibodies and T-cell receptors. Antigen-specific T-cell receptors and their reactions.Key Features* X-Ray Crystallography of Ligands* Catalytic Antibodies* Nature of the Antigen* Antibody Binding Sites* Maturtion of the Immune Response* Computational Biochemistry of Antibodies and * T-Cell Receptors* Antigen-Specific T-Cell Receptors and Their Reactions
Download or read book Antibodies Their structure and function written by M.W. Steward and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 97 pages. Available in PDF, EPUB and Kindle. Book excerpt: The student of biological science in his final years as an undergraduate and his first years as a graduate is expected to gain some familiarity with current research at the frontiers of his discipline. New research work is published in a perplexing diversity of publications and is inevitably con cerned with the minutiae of the subject. The sheer number of research journals and papers also causes confusion and difficulties of assimilation. Review articles usually presuppose a background knowledge of the field and are inevitably rather restricted in scope. There is thus a need for short but authoritative introductions to those areas of modern biological research which are either not dealt with in standard introductory textbooks or are not dealt with in sufficient detail to enable the student to go on from them to read scholarly reviews with profit. This series of books is designed to satisfy this need. The authors have been asked to produce a brief outline of their subject assuming that their readers will have read and remembered much of a standard introductory textbook of biology. This outline then sets out to provide by building on this basis, the conceptual framework within which modern research work is progressing and aims to give the reader an indication of the problems, both conceptual and practical, which must be overcome if progress is to be maintained.
Download or read book Molecular Biology of the Cell written by and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Monoclonal Antibody Production written by National Research Council and published by National Academies Press. This book was released on 1999-05-06 with total page 74 pages. Available in PDF, EPUB and Kindle. Book excerpt: The American Anti-Vivisection Society (AAVS) petitioned the National Institutes of Health (NIH) on April 23, 1997, to prohibit the use of animals in the production of mAb. On September 18, 1997, NIH declined to prohibit the use of mice in mAb production, stating that "the ascites method of mAb production is scientifically appropriate for some research projects and cannot be replaced." On March 26, 1998, AAVS submitted a second petition, stating that "NIH failed to provide valid scientific reasons for not supporting a proposed ban." The office of the NIH director asked the National Research Council to conduct a study of methods of producing mAb. In response to that request, the Research Council appointed the Committee on Methods of Producing Monoclonal Antibodies, to act on behalf of the Institute for Laboratory Animal Research of the Commission on Life Sciences, to conduct the study. The 11 expert members of the committee had extensive experience in biomedical research, laboratory animal medicine, animal welfare, pain research, and patient advocacy (Appendix B). The committee was asked to determine whether there was a scientific necessity for the mouse ascites method; if so, whether the method caused pain or distress; and, if so, what could be done to minimize the pain or distress. The committee was also asked to comment on available in vitro methods; to suggest what acceptable scientific rationale, if any, there was for using the mouse ascites method; and to identify regulatory requirements for the continued use of the mouse ascites method. The committee held an open data-gathering meeting during which its members summarized data bearing on those questions. A 1-day workshop (Appendix A) was attended by 34 participants, 14 of whom made formal presentations. A second meeting was held to finalize the report. The present report was written on the basis of information in the literature and information presented at the meeting and the workshop.
Download or read book Regulatory Peptides written by J.M. Polak and published by Birkhäuser. This book was released on 2013-03-07 with total page 419 pages. Available in PDF, EPUB and Kindle. Book excerpt: J. M. Polak and S. R. Bloom For some time Experientia has published, as a unique feature, interdis ciplinary multi-author reviews, giving a comprehensive overview of sub jects regarded as 'growing edges' of science. The enthusiasm shown by the readers was contagious and thus it was felt necessary to compile a special volume dealing with the novel aspects of regulatory peptides. This book covers some of the growing areas in regulatory peptide research and, although it is based on the original volume of Experientia, it is expanded and updated. The topic of 'regulatory peptides' is relatively young and has grown at an unprecedented pace, from the embryonic conception of 'gut hor mones' or 'brain neuropeptides' some 15 years ago to the realisation that these active pep tides are found, almost without exception, in every part of l8 23 the body in all vertebrate and many invertebrate species • Why the term 'regulatory peptides'? It represents a convenient label encompassing both the active peptides present in nerves, which are re leased as (putative) neurotransmitters, and those in endocrine cells, which act locally or at a distance as circulating hormones, these being the l8 main components of the so-called diffuse neuroendocrine or APUD 17 system • Morphological studies support this physiological viewpoint.
Download or read book Synthetic Peptides as Antigens written by Ruth Porter and published by John Wiley & Sons. This book was released on 2008-04-30 with total page 318 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Novartis Foundation Series is a popular collection of the proceedings from Novartis Foundation Symposia, in which groups of leading scientists from a range of topics across biology, chemistry and medicine assembled to present papers and discuss results. The Novartis Foundation, originally known as the Ciba Foundation, is well known to scientists and clinicians around the world.
Download or read book Immunobiology of Proteins and Peptides VI written by M. Zouhair Atassi and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 298 pages. Available in PDF, EPUB and Kindle. Book excerpt: The articles in this volume represent papers delivered by invited speakers at the 6th International Symposium on the Immunobiology of Proteins and Peptides. In addition, a few of the abstracts submitted by participants were scheduled for minisymposia and some of the authors, whose presentations were judged by the Scientific Council to be of high quality, were invited to submit papers for publication in this volume. This symposium was established in 1976 for the purpose of bringing together, once every two or three years, active investigators in the forefront of contemporary immunology, to present their findings and discuss t heir significance in the light of current concepts and to identify important new directions of investigation. The founding of the symposium was stimulated by the achievement of major breakthroughs in the understanding of the immune recognition of proteins and peptides. We believed that these breakthroughs will lead to the creation of a new generation of peptide reagents which should have enormous potential in biological, therapeutic and basic applications. This anticipated explosion has in fact since occurred and many applications of these pep tides are now being realized.
Download or read book Biochemistry of Antibodies written by R. Nezlin and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 395 pages. Available in PDF, EPUB and Kindle. Book excerpt: When the history of immunology in the twentieth century is written, the decade of the 1960's will, in all probability, stand out as the period of greatest advance in the development of molecular immunology. It is appropriate and useful, therefore, that a schol arly and integrated presentation of this progress should be made available in English. The translation of Dr. Nezlin's "Biochem istry of Antibodies" from Russian admirably fulfills this need in the form of a scientific monograph directed to medical and biolog ical scientists. The appearance of this monograph also serves to emphasize the conceptual unification of diverse immunological phenomena which has emerged from progress in molecular immunology. This unity is a consequence of the key role played by the antibody mol ecule (either in solution or cell-bound) in every biological process properly described as immunological. Indeed, immunology as an independent natural science can be described as the study of the structure, interactions, and biosynthes is of the antibody molecule.
Download or read book Phage Display of Peptides and Proteins written by Brian K. Kay and published by Elsevier. This book was released on 1996-10-23 with total page 369 pages. Available in PDF, EPUB and Kindle. Book excerpt: Both novices and experts will benefit from this insightful step-by-step discussion of phage display protocols.Phage Display of Peptides and Proteins: A Laboratory Manual reviews the literature and outlines the strategies for maximizing the successful application of phage display technology to one's research. It contains the most up-to-date protocols for preparing peptide affinity reagents, monclonal antibodies, and evolved proteins. - Prepared by experts in the field - Provides proven laboratory protocols, troubleshooting, and tips - Includes maps, sequences, and sample data - Contains extensive and up-to-date references
Download or read book Autoantibodies written by Yehuda Shoenfeld and published by Newnes. This book was released on 2013-11-26 with total page 917 pages. Available in PDF, EPUB and Kindle. Book excerpt: Autoimmune diseases are characterized by the occurrence of antibodies reacting with self-constituents of the body. The fully updated third edition of Autoantibodies is an in-depth review of the main autoantibodies identified up to now, with particular emphasis on those that display a diagnostic or prognostic clinical value. The new edition covers recent scientific advances, diagnostic techniques, and therapeutic technologies. Each chapter is focused on a single family of autoantibodies. This important reference contains historical notes, definitions, origins and sources of antigens recognized genetic associations, mediated pathogenic mechanisms, methods of detection, as well as clinical utility (disease prevalence and association, diagnostic value, sensitivity and specificity, prognostic value). This is an ideal reference for anyone involved in the field of autoimmune diseases. - Presents all known, important autoantibodies in a single source, focusing on the antibodies needed for autoimmune disorder diagnosis - Includes clinical applications for each autoantibody along with general information - Organized by disease and disorder type, by autoantibody family, and completely cross-referenced
Download or read book Antibodies written by G. Subramanian and published by Springer Science & Business Media. This book was released on 2013-03-07 with total page 260 pages. Available in PDF, EPUB and Kindle. Book excerpt: If the antibody industry is to achieve its full potential in the next decade, the individual technical potentials must be exploited, the limitations must be addressed, and lessons learned must be applied both to current purification methods and to the new technologies that continue to emerge. This book presents an overview of the current advances applied in the manufacture of monoclonal antibody including: -concepts in development of manufacturing strategies, -importance of antibody fragments, -application of chromatography method development, -quality control, -effect of expression on antibody properties, -virus removal and safety, -pharmacokinetics, -regulatory aspects.
