Download or read book PCR in Neuroscience written by and published by Elsevier. This book was released on 1995-04-13 with total page 455 pages. Available in PDF, EPUB and Kindle. Book excerpt: The volumes in this series include contemporary techniques significant to a particular branch of neuroscience. They are an invaluable aid to the student as well as the experienced researcher not only in developing protocols in neuroscience but in disciplines where research is becoming closely related to neuroscience. Each volume of Methods in Neurosciences contains an index, and each chapter includes references. Dr. Conn became Editor-in-Chief of the series beginning with Volume 15, so each subsequent volume could be guest-edited by an expert in that specific field. This further strengthens the depth of coverage in Methods in Neurosciences for students and researchers alike. Direct application of PCR to fresh or frozen clinical specimens (e.g., blood and solid tissue) Complete retrieval of novel expressed genes by PCR without screening a library Quantitation by PCR Mutagenesis by PCR PCR in AIDS research Simple and effective protocols for PCR on archival specimens

Download or read book In Situ Hybridization in Neurobiology written by James H. Eberwine and published by . This book was released on 1994 with total page 230 pages. Available in PDF, EPUB and Kindle. Book excerpt: In situ hybridization has become an important and widely used research tool for neurobiologists since the authors' first book on the subject, In Situ Hybridization: Applications to Neurobiology (Oxford, 1987). This new volume examines the methodological advances that have been made in in situhybridization techniques and their impact on the study of the nervous system. These include methods for improving sensitivity, ways of examining multiple mRNAs within the same section, ways of examining both proteins and mRNAs within the same section, and methods for using in situ hybridizationtechnology to examine areas of cellular biological significance, such as translational control. The development of alternative labels to radioactivity for the detection of hybridized probes, and the combined use of in situ hybridization and immunocytochemistry is also covered in this book. Itdiscusses the quantification of in situ hybridization signal and presents an overview of information generated by using in situ hybridization in diagnostic medicine. This volume will be essential to researchers in the neurosciences as well as those in other disciplines.

Download or read book Basic Molecular Protocols in Neuroscience Tips Tricks and Pitfalls written by John T. Corthell and published by Academic Press. This book was released on 2014-04-16 with total page 125 pages. Available in PDF, EPUB and Kindle. Book excerpt: Basic Neuroscience Protocols: Tips, Tricks, and Pitfalls contains explanatory sections that describe the techniques and what each technique really tells the researcher on a scientific level. These explanations describe relevant controls, troubleshooting, and reaction components for some of the most widely used neuroscience protocols that remain difficult for many neuroscientists to implement successfully. Having this additional information will help researchers ensure that their experiments work the first time, and will also minimize the time spent working on a technique only to discover that the problem was them, and not their materials. Describes techniques in very specific detail with step-by-step instructions, giving researchers in-depth understanding Offers many details not present in other protocol books Describes relevant controls for each technique and what those controls mean Chapters include references (key articles, books, protocols) for additional study Describes both the techniques and the habits necessary to get quality results, such as aseptic technique, aliquoting, and general laboratory rules

Download or read book Basic Science Methods for Clinical Researchers written by Morteza Jalali and published by Academic Press. This book was released on 2017-03-31 with total page 382 pages. Available in PDF, EPUB and Kindle. Book excerpt: Basic Science Methods for Clinical Researchers addresses the specific challenges faced by clinicians without a conventional science background. The aim of the book is to introduce the reader to core experimental methods commonly used to answer questions in basic science research and to outline their relative strengths and limitations in generating conclusive data. This book will be a vital companion for clinicians undertaking laboratory-based science. It will support clinicians in the pursuit of their academic interests and in making an original contribution to their chosen field. In doing so, it will facilitate the development of tomorrow’s clinician scientists and future leaders in discovery science. Serves as a helpful guide for clinical researchers who lack a conventional science background Organized around research themes pertaining to key biological molecules, from genes, to proteins, cells, and model organisms Features protocols, techniques for troubleshooting common problems, and an explanation of the advantages and limitations of a technique in generating conclusive data Appendices provide resources for practical research methodology, including legal frameworks for using stem cells and animals in the laboratory, ethical considerations, and good laboratory practice (GLP)

Download or read book Methods of Behavior Analysis in Neuroscience written by Jerry J. Buccafusco and published by CRC Press. This book was released on 2000-08-29 with total page 341 pages. Available in PDF, EPUB and Kindle. Book excerpt: Using the most well-studied behavioral analyses of animal subjects to promote a better understanding of the effects of disease and the effects of new therapeutic treatments on human cognition, Methods of Behavior Analysis in Neuroscience provides a reference manual for molecular and cellular research scientists in both academia and the pharmaceutic

Download or read book Cyclin Dependent Kinase 5 Cdk5 written by Nancy Y. Ip and published by Springer Science & Business Media. This book was released on 2009-02-28 with total page 326 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cyclin Dependent Kinase 5 provides a comprehensive and up-to-date collection of reviews on the discovery, signaling mechanisms and functions of Cdk5, as well as the potential implication of Cdk5 in the treatment of neurodegenerative diseases. Since the identification of this unique member of the Cdk family, Cdk5 has emerged as one of the most important signal transduction mediators in the development, maintenance and fine-tuning of neuronal functions and networking. Further studies have revealed that Cdk5 is also associated with the regulation of neuronal survival during both developmental stages and in neurodegenerative diseases. These observations indicate that precise control of Cdk5 is essential for the regulation of neuronal survival. The pivotal role Cdk5 appears to play in both the regulation of neuronal survival and synaptic functions thus raises the interesting possibility that Cdk5 inhibitors may serve as therapeutic treatment for a number of neurodegenerative diseases.

Download or read book Current Protocols in Neuroscience written by Jacqueline N. Crawley and published by Current Protocols. This book was released on 1997-06-01 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Current Protocols in Neuroscience (CPN) draws from techniques in molecular neurobiology, neurophysiology, neuroanatomy, neuropharmacology, and behavioral neuroscience to meet the specific needs of researchers in the full range of disciplines that is involved in studying the brain, nervous system, and corresponding behaviors. The editorial board of CPN have assembled an outstanding range of methods to enable users to explore their fields in greater depth and branch into related areas. The one-volume, looseleaf manual features carefully edited techniques with authors' troubleshooting tips and helpful comments that come from extensive experience in using these procedures. Quarterly updates, filed into the looseleaf, keep you and your laboratory current with the latest developments in this rapidly changing field. The initial purchase includes one year of updates and then subscribers may renew their annual subscriptions. Current Protocols publishes a family of laboratory manuals for bioscientists, including Molecular Biology, Immunology, Human Genetics, Protein Science, Cytometry, Cell Biology, Pharmacology, and Toxicology.

Download or read book Advances in Neuroscience and Schizophrenia written by A.H. Tuma and published by Springer Science & Business Media. This book was released on 2013-03-08 with total page 302 pages. Available in PDF, EPUB and Kindle. Book excerpt: Schizophrenia has been the subject of intense research interest in recent years, as investigators have explored the biological bases for the disorder and for various approaches to its diagnosis and treatment. This volume focuses on three aspects of such recent research connecting basic neuroscience to schizophrenia. In one, Professors Budinger, Gur, and Pettegrew provide critical reviews of brain imaging studies as they relate to cognitive behaviour functions in schizophrenia. In the second, Professors Goldmann-Rakic, Lewis, and Tassin discuss monoamine systems and their varied role in prefontal cortical circuitry and function. In the third, Professors Deutsch, Gerfen, and Grace discuss the structure, organization, and function of the basal ganglia, as they relate to schizophrenia and the mechanisms of neuroleptic action. These papers were presented at an interdisciplinary workshop on the subject at the University of Pittsburgh, in May 1991, and the discussion between the authors and other panelists in basic and clinical sciences are included in this volume as well. The presentation of these diverse approaches in an integrated fashion provides the reader with a unique perspective and a wealth of new questions for future collaborative research.

Download or read book Understanding PCR written by Sarah Maddocks and published by Academic Press. This book was released on 2016-10-27 with total page 98 pages. Available in PDF, EPUB and Kindle. Book excerpt: Understanding PCR: A Practical Bench-Top Guide gives you all of the information you need to plan your first PCR, from reagents to conditions to analysis and beyond. It is a user friendly book that has step-by-step basic protocols, which can be adapted to your needs. Includes helpful information such as where to order your reagents and basic troubleshooting hints and tips. Includes resources for reagents Explains basic laboratory preparation Provides straightforward experimental protocols Incorporates fundamental analytical techniques Contains a troubleshooting guide

Download or read book Current Laboratory Methods in Neuroscience Research written by Huangui Xiong and published by Springer. This book was released on 2016-08-24 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Current Laboratory Methods in Neuroscience Research is a research manual for both students and seasoned researchers. It focuses on commonly-used techniques employed in neuroscience research, presented in a simple, step-by-step manner for laboratory use. The manual also offers a “blueprint” for bench-to-bedside research designed to facilitate multidisciplinary neuroscience pursuits. Sections include coverage of neurohistological techniques, in vitro preparations, leukocyte isolation and application in neuroscience, standard laboratory nucleic acid and protein detections, nanomedicine, bioimaging, neuroelectrophysiology, immunohistochemistry and autoradiography, analysis of gene expression, and animal models.

Download or read book Encyclopedia of Neuroscience Volume 1 written by Larry R. Squire and published by Academic Press. This book was released on 2009-06-12 with total page 12505 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Encyclopedia of the Neuroscience explores all areas of the discipline in its focused entries on a wide variety of topics in neurology, neurosurgery, psychiatry and other related areas of neuroscience. Each article is written by an expert in that specific domain and peer reviewed by the advisory board before acceptance into the encyclopedia. Each article contains a glossary, introduction, a reference section, and cross-references to other related encyclopedia articles. Written at a level suitable for university undergraduates, the breadth and depth of coverage will appeal beyond undergraduates to professionals and academics in related fields.

Download or read book Neuroscience Methods written by Rosemary Martin and published by CRC Press. This book was released on 2019-08-16 with total page 280 pages. Available in PDF, EPUB and Kindle. Book excerpt: Unique in its coverage of such an extensive range of methods, Neuroscience Methods: A Guide for Advanced Students provides easy-to-understand descriptions of the many different techniques that are currently being used to study the brain at the molecular and cellular levels. This valuable reference text will help rescue undergraduate and postgraduate students from continuing bewilderment at the methods sections of current neuroscience publications. Topics covered include in vivo and in vitro preparations, electrophysiological, histochemical, hybridization and genetic techniques, measurement of cellular ion concentrations, methods of drug application, production of antibodies, expression systems, and neural grafting.

Download or read book Real time PCR written by M Dorak and published by Garland Science. This book was released on 2007-01-24 with total page 484 pages. Available in PDF, EPUB and Kindle. Book excerpt: With a variety of detection chemistries, an increasing number of platforms, multiple choices for analytical methods and the jargon emerging along with these developments, real-time PCR is facing the risk of becoming an intimidating method, especially for beginners. Real-time PCR provides the basics, explains how they are exploited to run a real-time PCR assay, how the assays are run and where these assays are informative in real life. It addresses the most practical aspects of the techniques with the emphasis on 'how to do it in the laboratory'. Keeping with the spirit of the Advanced Methods Series, most chapters provide an experimental protocol as an example of a specific assay.

Download or read book Guidelines for the Care and Use of Mammals in Neuroscience and Behavioral Research written by National Research Council and published by National Academies Press. This book was released on 2003-08-22 with total page 223 pages. Available in PDF, EPUB and Kindle. Book excerpt: Expanding on the National Research Council's Guide for the Care and Use of Laboratory Animals, this book deals specifically with mammals in neuroscience and behavioral research laboratories. It offers flexible guidelines for the care of these animals, and guidance on adapting these guidelines to various situations without hindering the research process. Guidelines for the Care and Use of Mammals in Neuroscience and Behavioral Research offers a more in-depth treatment of concerns specific to these disciplines than any previous guide on animal care and use. It treats on such important subjects as: The important role that the researcher and veterinarian play in developing animal protocols. Methods for assessing and ensuring an animal's well-being. General animal-care elements as they apply to neuroscience and behavioral research, and common animal welfare challenges this research can pose. The use of professional judgment and careful interpretation of regulations and guidelines to develop performance standards ensuring animal well-being and high-quality research. Guidelines for the Care and Use of Mammals in Neuroscience and Behavioral Research treats the development and evaluation of animal-use protocols as a decision-making process, not just a decision. To this end, it presents the most current, in-depth information about the best practices for animal care and use, as they pertain to the intricacies of neuroscience and behavioral research.

Download or read book Cellular and Molecular Methods in Neuroscience Research written by Adalberto Merighi and published by Springer Science & Business Media. This book was released on 2007-12-14 with total page 307 pages. Available in PDF, EPUB and Kindle. Book excerpt: There are numerous books on cellular and molecular protocols for general use in cell biology but very few are exclusively devoted to neurobiology. This book fills this gap and explains in a clear and consistent manner, some of the more commonly used protocols in neuroscience research. Each chapter is written by either the person who invented the procedure or an expert in the field. The format is uniform: "Overview," "Background," "Protocols," and "results and discussion." Each protocol begins with the principle of the technique, studies in cell culture, materials and reagents, and, lastly, step-by-step outline of the procedure itself. This highly practical book is also well illustrated (with 17 four color plates) to make the concepts and procedures easy to understand and perform.

Download or read book Methods in Alcohol Related Neuroscience Research written by Yuan Liu and published by CRC Press. This book was released on 2002-02-14 with total page 405 pages. Available in PDF, EPUB and Kindle. Book excerpt: Written by a panel of experts, Methods in Alcohol-Related Neuroscience Research not only provides information of a technical nature but also gives an overview of the many areas in investigating the effects of alcohol on the brain. It gives technical guidance for investigators doing research at the molecular, cellular, systems, and behavioral levels. These techniques include a wide variety of approaches, ranging from gene mapping and examination of molecular interactions of alcohol at the sub-cellular level to recording of neural activities in freely-behaving animals and imaging alcohol effects on the living human brain.

Download or read book Modern Techniques in Neuroscience Research written by Uwe Windhorst and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 1336 pages. Available in PDF, EPUB and Kindle. Book excerpt: An overview of the techniques used in modern neuroscience research with the emphasis on showing how different techniques can optimally be combined in the study of problems that arise at some levels of nervous system organization. This is essentially a working tool for the scientist in the laboratory and clinic, providing detailed step-by-step protocols with tips and recommendations. Most chapters and protocols are organized such that they can be used independently, while cross-references between the chapters, a glossary, a list of suppliers and appendices provide further help.