Download or read book PCR for detection of Campylobacter written by and published by Nordic Council of Ministers. This book was released on 2006 with total page 66 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book PCR for Detection of Campylobacter written by and published by . This book was released on 2006 with total page 63 pages. Available in PDF, EPUB and Kindle. Book excerpt: Abstract: Campylobacteriosis is currently the single most important cause of gastrointestinal disease in Denmark and several other European countries. One of the available tools for reducing the number of human infections is the implementation of strategic slaughter of broilers. Using real-time PCR for screening the broiler flocks for presence of Campylobacter would be advantageous for several reasons. Real-time PCR is more sensitive and specific than gel-based PCR, it is less work demanding, and the number of manual handling steps is reduced. The main aim of the present project was to disseminate the knowledge of an already validated real-time PCR method for detection of thermotolerant Campylobacter in samples from the chicken production line. The dissemination part of this project was carried out through a technology transfer trial with participation of central Nordic laboratories. The participating laboratories were assisted in implementing the real-time PCR method, and received samples, reagents and media for enrichment, DNA extraction and PCR. Detailed protocols on sample preparation and real-time PCR were prepared for each participant corresponding to their in-house real-time PCR equipment. Finally, based on the validation, and the results obtained in the technology transfer trial a standard proposal for the Nordic Committee on Food Analysis for detection of thermotolerant Campylobacter by real-time PCR was prepared

Download or read book CDC Yellow Book 2020 written by CENTERS FOR DISEASE CONTROL AND PREVENTION. (CDC) and published by Oxford University Press, USA. This book was released on 2019-06-11 with total page 721 pages. Available in PDF, EPUB and Kindle. Book excerpt: The definitive reference for travel medicine, updated for 2020! "A beloved travel must-have for the intrepid wanderer." -Publishers Weekly "A truly excellent and comprehensive resource." -Journal of Hospital Infection The CDC Yellow Book offers everything travelers and healthcare providers need to know for safe and healthy travel abroad. This 2020 edition includes: · Country-specific risk guidelines for yellow fever and malaria, including expert recommendations and 26 detailed, country-level maps · Detailed maps showing distribution of travel-related illnesses, including dengue, Japanese encephalitis, meningococcal meningitis, and schistosomiasis · Guidelines for self-treating common travel conditions, including altitude illness, jet lag, motion sickness, and travelers' diarrhea · Expert guidance on food and drink precautions to avoid illness, plus water-disinfection techniques for travel to remote destinations · Specialized guidelines for non-leisure travelers, study abroad, work-related travel, and travel to mass gatherings · Advice on medical tourism, complementary and integrative health approaches, and counterfeit drugs · Updated guidance for pre-travel consultations · Advice for obtaining healthcare abroad, including guidance on different types of travel insurance · Health insights around 15 popular tourist destinations and itineraries · Recommendations for traveling with infants and children · Advising travelers with specific needs, including those with chronic medical conditions or weakened immune systems, health care workers, humanitarian aid workers, long-term travelers and expatriates, and last-minute travelers · Considerations for newly arrived adoptees, immigrants, and refugees Long the most trusted book of its kind, the CDC Yellow Book is an essential resource in an ever-changing field -- and an ever-changing world.

Download or read book Development of a Real time PCR Assay for the Detection of Campylobacter Jejuni and Campylobacter Coli written by and published by . This book was released on 2009 with total page 105 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Specific Detection of Campylobacter Jejuni and Campylobacter Coli by Using Polymerase Chain Reaction written by and published by . This book was released on 1992 with total page 8 pages. Available in PDF, EPUB and Kindle. Book excerpt: Development of a routine detection assay for Campylobacter jejuni and Campylobacter coli in clinical specimens was undertaken by using the polymerase chain reaction(PCR). An oligonucleotide primer pair from a conserved 5' region of the flaA gene of C. coli VC167 was used to amplify a 450-bp region by PCR. The primer pair specifically detected 4 strains of C.coli and 47 strains C. jejuni; but it did not detect strains of Campylobacter fetus, Campylobacter lari, Campylobacter upsaliensis, Campylobacter cryaerophila, Campylobacter butzleri, Campylobacter hyointestinalis, Wolinella recta, Helicobacter pylori, Escherichia coli, Shigella spp., Salmonella spp., Bibrio cholerae, Citrobacter freundii, or Aeromonas spp. By using a non radioactively labeled probe internal to the PCR product, the assay could detect as little as 0.0062 pg of purified C. coli in DNA, or the equivalent of four bacteria. In stools steeded with C. coli cells, the probe could detect between 30 and 60 bacteria per PCR assay. the assay was also successfully used to detect C. coli in rectal swab specimens form experimentally infected rabbits and C.jejuni in human stool samples.

Download or read book Isolation and PCR Based Detection of Campylobacter Jejuni and Campylobacter Fetus in Man Animals and Foods of Animal Origin written by Harkanwal Deep Singh and published by . This book was released on 2006 with total page 91 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book The Development and Optimization of a RT PCR Assay for Rapid Campylobacter Spp Detection and Quantification in Poultry Rinsates written by Aaron Bodie and published by . This book was released on 2022 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Campylobacter is a foodborne pathogen mainly associated with poultry products. Currently, prevalence-based data is used to determine process control during poultry processing. However, this method is not sensitive enough, labor intensive and does not provide information on the level and subsequent risk of Campylobacter contamination. As a result, reliable alternative methods are imperative to detect and quantify Campylobacter contamination throughout the harvest process. Therefore, the goal of this dissertation was to develop and optimize a real-time polymerase chain reaction (RT-PCR) assay for rapid detection and quantification of Campylobacter spp. in poultry rinsates using the United States Department of Agriculture - Food Safety Inspection Services protocol and sampling techniques. The first project was focused on identifying the compositional diversity of pooled colonies enumerated from post-chill poultry carcass samples inoculated with Campylobacter jejuni, coli, and lari on various selective and non-selective media, Campy-Cefex, mCCDA, and TSA; (Chapter 2). The objective of the second study was to generate growth curves for Campylobacter jejuni, coli, and lari under the same growth conditions recommended by the International standard organization (Chapter 3). The last chapter aimed to develop and verify a rapid quantification method for Campylobacter species (CampyQuant[TM]) in post-chill poultry rinsates using the BAX® System Real-Time PCR Assay for Campylobacter (Chapter 4). The results of this dissertation were that BAX® System CampyQuant[TM] assay was able to detect Campylobacter jejuni, coli, and lari after 20 h enrichment and quantify the pre-enrichment concentration in a range from 1.00 to 4.00 Log10 CFU/mL. The study suggests that the CampyQuant[TM] BAX® RT-PCR assay can provide the food industry with a rapid, accurate, and efficient alternative method for Campylobacter spp. enumeration to ensure that process controls are working adequately to provide safe products to consumers.

Download or read book Detection and identification of campylobacter spp using the polymerase chain reaction written by Belinda Anna Johanna Giesendorf and published by . This book was released on 1994 with total page 117 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Campylobacter Jejuni written by Irving Nachamkin and published by Amer Society for Microbiology. This book was released on 1992 with total page 300 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book the first major text on Camplyobacter infections in over 8 years, summarizes the major advances in understanding the clinical disease and epidemiology of infection. Probably the most common cause of sporadic bacterial diarrheal illness, this pathogen accounts for an estimated 2.4 million cases annually in the United States.

Download or read book Real time PCR Detection and Speciation for Campylobacter written by Great Britain. Public Health Laboratory Service and published by . This book was released on 2000 with total page 36 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book PCR Detection of Microbial Pathogens written by Konrad Sachse and published by Springer Science & Business Media. This book was released on 2003 with total page 667 pages. Available in PDF, EPUB and Kindle. Book excerpt: Hands-on laboratory experts present a set of "classic" PCR-based methods for the identification and detection of important animal and food microbial pathogens, including several zoonotic agents. These proven techniques can be precisely applied to a wide variety of microbes, among them Campylobacter spp., chlamydiae, toxigenic clostridia, Escherichia coli (STEC), Listeria monocytogenes, mycoplasmas, salmonellae, and Yersinia enterocolitica. Additional chapters review the specificity and performance of diagnostic PCR analysis, the pre-PCR processing of samples, the critical aspects of standardizing PCR methods, and the general issues involved in using PCR technology for microbial diagnosis.

Download or read book Bacteriological Analytical Manual written by United States. Food and Drug Administration. Division of Microbiology and published by . This book was released on 1969 with total page 180 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Magnetic Nanoparticle Based PCR Assay for Detection of Campylobacter Species in Food Samples and Dielectrophoretic Impedance Measurement for DNA Detection written by Wuttichote Jansaento and published by . This book was released on 2014 with total page 164 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book A Real time PCR Assay for the Rapid Detection and Quantification of Campylobacter Jejuni in Broilers written by Aradom Debretsion and published by . This book was released on 2006 with total page 55 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book PCR Detection of Microbial Pathogens written by Mark Wilks and published by Humana Press. This book was released on 2016-08-23 with total page 317 pages. Available in PDF, EPUB and Kindle. Book excerpt: PCR methods for the detection of microbial pathogens have made relatively little impact in diagnostic microbiology laboratories due to the common decision to use expensive commercially produced tests rather than the cheaper alternative of developing one’s own tests or introducing tests developed by other workers. PCR Detection of Microbial Pathogens, Second Edition presents alternatives to commercially produced PCR methods to detect microbial pathogens. Although most of the chapters in this book are devoted to the detection of specific pathogens, the first chapters in this book should appeal to anyone working in this field regardless of their particular interests. Although PCR tests can often be made to work with relatively little effort, it is often unclear how efficient the PCR test is, how inhibitory the specimen containing the pathogen of interest is and how the test can be quality controlled. All of which are of great importance in developing tests for diagnostic use. These topics are covered in great depth at the beginning of the book. The main part of the book is devoted to describing methods for the detection of a wide range of pathogens and from widely different specimens and situations. Written in the highly successful Methods in Molecular BiologyTM series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, PCR Detection of Microbial Pathogens, Second Edition serves microbiologists regardless of their particular interest because, when used together with the general principles, the sheer variety of procedures provided here enables the reader to design and introduce diagnostic tests in the laboratory with confidence.

Download or read book Detection of Campylobacter Fetus in Bovine Preputial Scrapings Using PCR and Culture Assays written by Tracy Schmidt and published by . This book was released on 2008 with total page 216 pages. Available in PDF, EPUB and Kindle. Book excerpt: The traditional method for the diagnosis of bovine genital campylobacteriosis is the culture and identification of the causative organism, Campylobacter fetus subsp. venerealis (Cfv) from the genital tract. This approach is considered relatively insensitive due to the fragility of the bacteria, their specific nutritional and atmospheric requirements and their being easily overgrown by commensal bacteria. The identification of isolates is also problematic due to the limited biochemical activity of the bacteria. With the rapid advances made in the molecular field, assays have become more robust and cost-effective making them feasible for the diagnostic laboratory. The potential speed, sensitivity and specificity offered by these assays provide attractive alternatives for the identification of pathogens which are notoriously difficult to identify. The first part of this investigation was concerned with the implementation and evaluation of a polymerase chain reaction (PCR) assay for the direct detection of C. fetus in bovine preputial specimens. The specificity of a published C. fetus-specific primer pair was established by testing C. fetus reference and field isolates in addition to a collection of other Campylobacter species and organisms which may encountered in the genital tract of cattle. All C. fetus isolates tested yielded a single PCR amplicon of approximately 750 bp. No amplicons were generated when any of the other non-C. fetus isolates were tested. Following minor modifications to the assay, the sensitivity of the assay was determined using spiked Weybridge medium. A detection limit of 615 Cfv/mR Weybridge medium (or 6,15 cell equivalents per PCR assay) was obtained. Preputial material collected and submitted for laboratory testing may often be contaminated with faeces, urine, semen and/or blood. All of these components are known to be potential PCR inhibitors and the influence of each, on the sensitivity of the PCR assay, was subsequently evaluated. Faeces were identified as a potent inhibitor and contamination of specimens with as little as 1% (w/v) faeces reduced the sensitivity of the assay. Concentrations of up to 50% (v/v) of blood, urine and semen had no effect on the sensitivity of the assay. Preputial specimens, collected in Weybridge medium, were subsequently pooled and spiked and used to establish the sensitivity of both the PCR and culture methods as well as determine the influence of time on the sensitivity of the assays. Testing was carried out in triplicate on samples collected from different herds which were ascertained to be free of Cfv based on the use of specific selection criteria. The detection limit of the culture method was found to be better than that achieved using PCR only immediately after the samples were spiked. The detection limit of the culture method decreased with time whilst the detection limit of the PCR assay remain unchanged up to 72 hours post-inoculation. Ensuing field evaluation involved the testing of 212 clinical samples using both the culture method and the optimized PCR assay. Of the samples tested 4,2% were found to be positive using the PCR assay, whilst only 3,8% were found to be positive by culture. Based upon this evaluation the analytical specificity of the PCR assay was calculated to be 99% and the analytical sensitivity, 85,7%. The second part of this investigation was concerned with the subspeciation of C. fetus isolates. Currently the only test recommended by the Office International des Epizooties (OIE) for the subspeciation of isolates, is tolerance to 1% glycine. Doubts over the reliability of this test have led to alternative or supplementary tests being sought. Within the context of this investigation a collection of 40 South African field isolates were subspeciated using a previously described subspecies-specific primer set as well as the traditional 1% glycine tolerance phenotyping test. Additionally, other phenotyping tests (selenite reduction, growth at 42 °C and susceptibility to metronidazole and cefoperazone) were evaluated to determine their suitability for use as an aid in the subspeciation of C. fetus isolates. None of the field isolates yielded a Cfv-specific subspecies PCR amplicon using the published primer set suggesting that all of the isolates were Campylobacter fetus subsp. fetus (Cff). Based on tolerance to 1% glycine however, only 6 isolates were identified as Cff (glycine tolerant), whilst the remainder were classified as Cfv. The results of the "sensitive" hydrogen sulphide test indicated that the Cfv isolates were specifically Cfv biovar intermedius. The lack of agreement between the PCR and the phenotyping subspeciation results concur with the findings reported by other researchers. It is consequently concluded that the published VenSF/VenSR subspecies-primer set is unsuitable for the subspeciation of South African field isolates.

Download or read book Detection of Campylobacter Spp and Complobacter Jejuni in Raw Vegetables by Using Direct Polymerase Chain Reaction written by and published by . This book was released on 2010 with total page 46 pages. Available in PDF, EPUB and Kindle. Book excerpt: