Download or read book Optimization of In Vitro Tissue Culture and Transformation System for Sweet Sorghum Cultivars Using Immature Inflorescence written by Logayn Abushal and published by . This book was released on 2021 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Abstract: Background: Sweet sorghum is a multi-purpose crop that can be used for human food, animal feed, and bio-fuel production. This crop is drought-tolerant and can therefore be grown in arid and semi-arid regions especially in marginal areas in Egypt. Nevertheless, production of Sweet sorghum cannot coup with the increasing demand in consumption as a food, and fuel due to a lag in scientific research regarding its genetic improvement. Therefore, genetic improvement of Sweet sorghum is innately required so that new traits of economic importance could be introduced; however, this can only be achieved through the establishment of a robust in-vitro tissue culture system. Thus, this study aimed to establish an effective regeneration system for three different Sweet sorghum cultivars namely; Rex, Sugar Drip, and Ramada, using immature inflorescence explants. Subsequently, the cultivar Sugar was selected to examine its transformation efficiency using the microprojectile bombardment technology. Methods: For callus induction media, explants from immature inflorescences of different lengths (1.5- 16 cm), were grown over a combination of 2,4-D (0, 2, 4 & 6mg/L) with kinetin (0, 0.2 & 0.5mg/L). For regeneration media, BAP was the main cytokinin implemented in the three different treatments along with either: NAA, IAA, or IAA & TDZ, whereas TDZ was employed alone in a fourth treatment. The transformation system of the cultivar Sugar Drip was also developed using immature inflorescence explants. Co-bombardment was performed using the neomycin phosphotransferase II (emnptII) gene under the control of maize ubiquitin (emUbi1) promoter. The selection of putative transgenic plants was performed using paromomycin antibiotic. Results: The best embryogenic callus induction frequency was observed in Rex (77%) on modified MS media supplemented with 4mg/L 2,4-D + 0.2mg/L kinetin after six weeks from culture. However, Ramada and Sugar Drip had their highest callus induction rates of 93 and 94%, respectively, when 6mg/L 2,4-D + 0.2mg/L kinetin was used. The addition of 1mg/L IAA + 0.5mg/L BAP + 0.1mg/L TDZ had its best outcome in terms on shoot induction, shoot/callus number, and root formation. In Sugar Drip transformation, paromomycin eliminated most the non-transgenic plants from the putative transgenic ones. Eventually, 6 out of 348 bombarded samples were found to be transgenic after PCR screening. The percentage of transformation from two independent experiments was around 1.724%. Conclusion: PCR analysis of putative transformants revealed a transformation efficiency of 1.724%. Therefore, genetic transformation using particle bombardment has shown to be a successful method for the transformation of immature Sweet sorghum inflorescences with relatively high success rates.
Download or read book Bibliography of Agriculture written by and published by . This book was released on 1998 with total page 2312 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Plant Propagation by Tissue Culture In practice written by Edwin F. George and published by . This book was released on 1993 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Neglected Crops written by J. Esteban Hernández Bermejo and published by Food & Agriculture Org.. This book was released on 1994 with total page 372 pages. Available in PDF, EPUB and Kindle. Book excerpt: About neglected crops of the American continent. Published in collaboration with the Botanical Garden of Cord�ba (Spain) as part of the Etnobot�nica92 Programme (Andalusia, 1992)
Download or read book Date Palm Biotechnology written by Shri Mohan Jain and published by Springer Science & Business Media. This book was released on 2011-08-13 with total page 745 pages. Available in PDF, EPUB and Kindle. Book excerpt: This important reference book is the first comprehensive resource worldwide that reflects research achievements in date palm biotechnology, documenting research events during the last four decades, current status, and future outlook. This book is essential for researchers, policy makers, and commercial entrepreneurs concerned with date palm. The book is invaluable for date palm biotechnology students and specialists. This monument is written by an international team of experienced researchers from both academia and industry. It consists of five sections covering all aspects of date palm biotechnology including A) Micropropagation, B) Somaclonal Variation, Mutation and Selection, C) Germplasm Biodiversity and Conservation, D) Genetics and Genetic Improvement, and E) Metabolites and Industrial Biotechnology. The book brings together the principles and practices of contemporary date palm biotechnology. Each chapter contains background knowledge related to the topic, followed by a comprehensive literature review of research methodology and results including the authors own experience including illustrative tables and photographs.
Download or read book The Flowering of Apomixis written by Yves Savidan and published by CIMMYT. This book was released on 2001 with total page 260 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Plant Comparative Genomics written by Alejandro Pereira-Santana and published by Springer Nature. This book was released on 2022-07-11 with total page 293 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed book presents recent methodologies for the task of inspecting the genomic world of plants, extracting valuable information, and presenting it in a readable way. With a focus on bioinformatics tools, the volume explores phylogenetics and evolution, Omics analysis, as well as experimental procedures for trait characterization. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of vital expert implementation advice that will lead to successful results. Authoritative and practical, Plant Comparative Genomics serves as an ideal resource for researchers looking to implement comparative tools in order to explore their genomic data for their daily scientific work.
Download or read book Plant Biotechnology and Genetics written by C. Neal Stewart, Jr. and published by John Wiley & Sons. This book was released on 2012-12-13 with total page 554 pages. Available in PDF, EPUB and Kindle. Book excerpt: Designed to inform and inspire the next generation of plant biotechnologists Plant Biotechnology and Genetics explores contemporary techniques and applications of plant biotechnology, illustrating the tremendous potential this technology has to change our world by improving the food supply. As an introductory text, its focus is on basic science and processes. It guides students from plant biology and genetics to breeding to principles and applications of plant biotechnology. Next, the text examines the critical issues of patents and intellectual property and then tackles the many controversies and consumer concerns over transgenic plants. The final chapter of the book provides an expert forecast of the future of plant biotechnology. Each chapter has been written by one or more leading practitioners in the field and then carefully edited to ensure thoroughness and consistency. The chapters are organized so that each one progressively builds upon the previous chapters. Questions set forth in each chapter help students deepen their understanding and facilitate classroom discussions. Inspirational autobiographical essays, written by pioneers and eminent scientists in the field today, are interspersed throughout the text. Authors explain how they became involved in the field and offer a personal perspective on their contributions and the future of the field. The text's accompanying CD-ROM offers full-color figures that can be used in classroom presentations with other teaching aids available online. This text is recommended for junior- and senior-level courses in plant biotechnology or plant genetics and for courses devoted to special topics at both the undergraduate and graduate levels. It is also an ideal reference for practitioners.
Download or read book Biotechnology and Sustainable Agriculture 2006 and Beyond written by Zhihong Xu and published by Springer. This book was released on 2010-10-19 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This timely work is a collection of papers presented at the XIth international congress of the International Association of Plant Tissue Culture & Biotechnology. It continues the tradition of the IAPTC&B in publishing the proceedings of its congresses. The work is an up-to-date report on the most significant advances in plant tissue culture and biotechnology as presented by leading international scientists. It will be crucial reading for agricultural scientists, among others.
Download or read book Plant Cell Tissue and Organ Culture written by Oluf Gamborg and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 367 pages. Available in PDF, EPUB and Kindle. Book excerpt: This manual provides all relevant protocols for basic and applied plant cell and molecular technologies, such as histology, electron microscopy, cytology, virus diagnosis, gene transfer and PCR. Also included are chapters on laboratory facilities, operation and management as well as a glossary and all the information needed to set up and carry out any of the procedures without having to use other resource books. It is especially designed for professionals and advanced students who wish to acquire practical skills and first-hand experience in plant biotechnology.
Download or read book Cotton Research written by Ibrokhim Y. Abdurakhmonov and published by BoD – Books on Demand. This book was released on 2016-11-09 with total page 312 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cotton is the most important natural fiber crop of our planet, which provides humanity with cloth and vegetable oil, medicinal compounds, meal and hull for livestock feed, energy sources, organic matter to enrich soil, and industrial lubricants. Therefore, cotton research to improve sustainable cotton production worldwide is the vital task of scientific community to address the increasing demands and needs for cotton products. This Cotton Research book presents readers updated information and advances in current cotton science investigations. Chapters of this book provide the latest developments on cotton research and cover topics on cotton research infrastructure, physiology and agronomy, breeding and genetics, modern biotechnology, genomics and molecular breeding, crop management, and cotton-based product and textile researches.
Download or read book Protocols for Micropropagation of Selected Economically Important Horticultural Plants written by Maurizio Lambardi and published by Humana Press. This book was released on 2012-09-24 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Micropropagation is a reliable technology applied commercially worldwide for large-scale plant multiplication, germplasm conservation, pathogen elimination, genetic manipulations and supply of selected plants. In Protocols for Micropropagation of Selected Economically-Important Horticultural Plants, well recognised researchers in the field compile step-wise protocols for rapid plant multiplication of economically-important horticultural species. The book contains 35 chapters, divided into four major sections. The first three sections (Section A, B and C) contain 29 micropropagation protocols of selected fruit and nut species, indoor and outdoor ornamental plants, cut flowers, and vegetables. In addition to the detailed protocols of in vitro shoot initiation, proliferation, root induction and acclimatization, chapters also include detailed information on medium preparation, explant selection and preparation. The six chapters of Section D cover specific reviews on pivotal topics, such as in vitro rejuvenation, synthetic seed technology, thermotherapy and meristem culture in banana, genetic transformation of pineapple, flower color somaclonal variation in torenia, and cryotherapy of horticultural crops. Moreover, as a part of the highly successful Methods in Molecular Biology series, chapters include introductions to the respective topic, lists of necessary materials, notes, and illustrative photos. Comprehensive and well-written, Protocols for Micropropagation of Selected Economically-Important Horticultural Plants offers a useful resource for horticulturists, researchers, commercial companies, plant propagators, biotechnologists and students interested in micropropagation.
Download or read book Breeding Sorghum for Diverse End Uses written by Aruna C and published by Woodhead Publishing. This book was released on 2018-08-22 with total page 450 pages. Available in PDF, EPUB and Kindle. Book excerpt: Breeding Sorghum for Diverse End Uses is a comprehensive overview of all significant global efforts for the genetic improvement of sorghum, a major crop of many semi-arid nations that is suitable for a huge range of uses, from human food, to biofuels. Split into two main sections, the book initially reviews the genetic suitability of sorghum for breeding, also providing the history of the genetic improvement of the grain. Finally, other sections look at specific breeding programs that could be improved in a number of areas, including human food, animal feed and industrial usage. Readers in academics, research, plant genetics and sorghum development will find this resource of great value. In addition, it is essential reading for engineers who utilize sorghum for food, feed and industrial materials in industry. Provides information on key advances in the genetic makeup of sorghum Allows plant breeders to apply this research to effectively breed new strains of sorghum that are dependent on final usage goals Includes the latest findings in each section to orient researchers to plans for future genetic enhancement
Download or read book Berry Fruit written by Yanyun Zhao and published by CRC Press. This book was released on 2007-06-06 with total page 444 pages. Available in PDF, EPUB and Kindle. Book excerpt: Valued for its unique flavors, textures, and colors, berry fruit is a rapidly-growing crop that provides significant health benefits due to its many bioactive components. Berry Fruit: Value-Added Products for Health Promotion provides an understanding of the functional chemicals, shelf-life and microbial safety concerns, and applications in the food industry. Written by international experts, each chapter presents scientific background, research results, critical reviews of relevant issues, and case studies. Chapters include worldwide production information, bioactive food constituents and their potential health benefits, methods of enhancing food quality, and potential uses of berry processing by-products.
Download or read book Handbook of Bioenergy Crops written by N. El Bassam and published by Earthscan. This book was released on 2010 with total page 545 pages. Available in PDF, EPUB and Kindle. Book excerpt: This completely revised second edition includes new information on biomass in relation to climate change, new coverage of vital issues including the "food versus fuel" debate, and essential new information on "second generation" fuels and advances in conversion techniques. The book begins with a guide to biomass accumulation, harvesting, transportation and storage, as well as conversion technologies for biofuels. This is followed by an examination of the environmental impact and economic and social dimensions, including prospects for renewable energy. The book then goes on to cover all the main potential energy crops.
Download or read book Handbook of Banana Production Postharvest Science Processing Technology and Nutrition written by Muhammad Siddiq and published by John Wiley & Sons. This book was released on 2020-09-15 with total page 288 pages. Available in PDF, EPUB and Kindle. Book excerpt: A comprehensive guide that covers the banana’s full value chain – from production to consumption The banana is the world’s fourth major fruit crop. Offering a unique and in-depth overview of the fruit’s entire value chain, this important new handbook charts its progression from production through to harvest, postharvest, processing, and consumption. The most up-to-date data and best practices are drawn together to present guidelines on innovative storage, processing, and packaging technologies, while fresh approaches to quality management and the value-added utilization of banana byproducts are also explained. Additionally, the book examines the banana’s physiology, nutritional significance, and potential diseases and pests. The book also Edited by noted experts in the field of food science, this essential text: Provides a new examination of the world’s fourth major fruit crop Covers the fruit’s entire value chain Offers dedicated chapters on bioactive and phytochemical compounds found in bananas and the potential of processing byproducts Gives insight into bananas’ antioxidant content and other nutritional properties Identifies and explains present and possible effects of bioactive and phytochemical compounds Handbook of Banana Production, Postharvest Science, Processing Technology, and Nutrition offers the most far-reaching overview of the banana currently available. It will be of great benefit to food industry professionals specializing in fruit processing, packaging, and manufacturing banana-based products. The book is also an excellent resource for those studying or researching food technology, food science, food engineering, food packaging, applied nutrition, biotechnology, and more.
Download or read book Plant Mutation Breeding and Biotechnology written by Q. Y. Shu and published by CABI. This book was released on 2012 with total page 612 pages. Available in PDF, EPUB and Kindle. Book excerpt: Abstract: This book presents contemporary information on mutagenesis in plants and its applications in plant breeding and research. The topics are classified into sections focusing on the concepts, historical development and genetic basis of plant mutation breeding (chapters 1-6); mutagens and induced mutagenesis (chapters 7-13); mutation induction and mutant development (chapters 14-23); mutation breeding (chapters 24-34); or mutations in functional genomics (chapters 35-41). This book is an essential reference for those who are conducting research on mutagenesis as an approach to improving or modifying a trait, or achieving basic understanding of a pathway for a trait --.
