Download or read book Optical Methods for Structure Elucidation of Protein ligand Interactions Fluorescence and Ultraviolet Resonance Raman Spectroscopy written by Silvia Tardioli and published by . This book was released on 2011 with total page 197 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Protein ligand Interactions Structure and Spectroscopy written by Stephen E. Harding and published by Oxford University Press, USA. This book was released on 2001 with total page 474 pages. Available in PDF, EPUB and Kindle. Book excerpt: This text on protein-ligand interactions offers a selection of the most useful and easily applied methods and acts as a guide to the principal techniques used.
Download or read book Ultraviolet Spectroscopy of Proteins written by Alexander P. Demchenko and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 323 pages. Available in PDF, EPUB and Kindle. Book excerpt: The aim of this book is to give a comprehensive description of the basic methods used in the ultraviolet spectroscopy of proteins, to discuss new trends and development of these methods, and to analyze their different applications in the study of various aspects of protein structure and dynamics. Ultraviolet spectroscopy is one of the oldest and most popular methods in the field of biochemistry and molecular biophysics. At present, it is difficult to imagine the biochemical laboratory without a recording spectrophotometer or spectrofluorimeter. There are several hundreds of publications directly devoted to protein ultraviolet spectroscopy and in a great number of studies UV spectroscopic methods are used for the structural analysis of different proteins. Meanwhile a unified description of the theoretical basis of the methods, experimental techniques, data analysis, and generalization of results obtained in solving the specific problems of protein structure are lacking. There are three reasons for which a monograph on ultraviolet spectroscopy is needed today. Firstly, there has been significant growth in facilities of experimental technique, its precision, and versatility associated with computer data analysts. This new technique is available to a wide circle of scientists engaged in the field of protein research. Most of them are not spectroscopists and, thus, there is a need for a conceivable and precise source of information on how to use this method and what kind of data it should provide.
Download or read book Methods in Protein Structure and Stability Analysis written by Vladimir N. Uversky and published by Nova Publishers. This book was released on 2007 with total page 382 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Fluorescence and UV Resonance Raman Spectroscopy Studies of HU Protein Binding to DNA written by Kristi Wojtuszewski Poulin and published by . This book was released on 2002 with total page 398 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Ultraviolet Resonance Raman and Fluorescence Studies of Folded and Unfolded Conformations of the Membrane Protein OmpA written by Katheryn Marie Sanchez and published by . This book was released on 2010 with total page 230 pages. Available in PDF, EPUB and Kindle. Book excerpt: This dissertation focuses on the folding dynamics of a bacterial membrane protein, Outer Membrane Protein A (OmpA), using fluorescence and Ultraviolet resonance Raman spectroscopy. Our model [Beta]-barrel membrane protein, OmpA, contains five native anchoring Tryptophan residues. The spectroscopic properties of trp residues are highly sensitive to the local environment, making it an ideal probe for membrane protein folding studies. Utilizing trp fluorescence, refolding studies were performed on single trp mutants of OmpA to determine the thermodynamic stability of these trp mutants. The important noncovalent interactions that promote stability in OmpA are pairwise aromatic interactions and hydrogen bonds with the N1H moiety of trp. Refolding studies were also performed on truncated single-trp mutants, in which the soluble domain of the protein was removed. These studies resulted in increased stability relative to the full-length protein and suggest the absence of the soluble domain may destabilize the unfolded transmembrane domain. Ultraviolet resonance Raman spectroscopy (UVRR) is a powerful vibrational technique that can selectively probe different biological chromophores depending on excitation wavelength. Excitation wavelength dependence studies were performed on OmpA using wavelengths from 206.5 nm - 236.5 nm. This study determined an optimal excitation wavelength of 228-nm to selectively enhance signal from trp residues in OmpA. Additionally, UVRR was used to monitor changes in trp environmental hydrophobicity, hydrogen bonding, and dihedral torsion angle in different conformations of OmpA. The first UVRR spectra were collected of OmpA in a highly scattering environment and show differences in folded and unfolded conformations of the protein, showing the applicability of this technique to study membrane protein folding. UVRR spectra were collected of trp mutants of OmpA at different time points in the folding/insertion process to determine the types of noncovalent interactions with trp residues, and the folding timescales these interactions occur. Our results indicate noncovalent interactions start to form within the first 20 minutes after initiation of folding into DMPC vesicles and continue to show subtle changes over the course of the folding process. Additionally, there is evidence for interactions between trp residues and lipids, inter-residue hydrogen bonding, and amino-aromatic interactions.
Download or read book Cumulated Index Medicus written by and published by . This book was released on 1994 with total page 1516 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Excited States of Biopolymers written by Robert Steiner and published by Springer. This book was released on 1983-09-01 with total page 258 pages. Available in PDF, EPUB and Kindle. Book excerpt: During the past decade, fluorescence techniques have come to occupy a position of central importance in biochemistry. Such areas as laser techniques, radiation less energy transfer, and nanosecond fluorometry have evolved from esoteric research specialties into standard procedures that are applied routinely to bio chemical problems. Indeed, discussion of the above three areas occupies the greater part of this book. Its level and approach are appropriate for the bio logical or physical scientist who is interested in applying fluorescence techniques, but is not necessarily an expert in this area. The coverage of the literature has, in general, been selective rather than exhaustive. It is likely that what is summarized here will prove resistant to the erosion of time and provide a basis for the future evolution of this rapidly developing area of science. Robert F. Steiner Catonsville, Maryland vii Contents Chapter 1 Some Principles Governing the Luminescence of Organic Molecules R. M Hochstrasser 1. Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 2. Spontaneous Emission . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 2. 1. General Considerations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 2. 2. Luminescence from Nearby States . . . . . . . . . . . . . . . . . . . . . . . . . 4 2. 3. Multiple State Decay . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 3. Molecular Luminescence Characteristics . . . . . . . . . . . . . . . . . . . . . . . . . 5 3. 1. The Transition Dipole Moment . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 3. 2. Determination of Transition-Moment Directions from Fluorescence . . . 8 3. 3. Polarization of Fluorescence from Crystals. . . . . . . . . . . . . . . . . . . . 9 4. Principles of Luminescence Experiments Carried Out with Lasers. . . . . . . 12 4. 1. Nonlinear Processes and Optical Pumping . . . . . . . . . . . . . . . . . . . 12 5. Coherent Interactions of Molecules and Light . . . . . . . . . . . . . . . . . . . . 16 5. 1. The Distinctions between Fluorescence and Resonance Raman Effects 17 6. Ultrafast Fluorescence Decay . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Download or read book Visualizing Chemistry written by National Research Council and published by National Academies Press. This book was released on 2006-06-01 with total page 222 pages. Available in PDF, EPUB and Kindle. Book excerpt: Scientists and engineers have long relied on the power of imaging techniques to help see objects invisible to the naked eye, and thus, to advance scientific knowledge. These experts are constantly pushing the limits of technology in pursuit of chemical imagingâ€"the ability to visualize molecular structures and chemical composition in time and space as actual events unfoldâ€"from the smallest dimension of a biological system to the widest expanse of a distant galaxy. Chemical imaging has a variety of applications for almost every facet of our daily lives, ranging from medical diagnosis and treatment to the study and design of material properties in new products. In addition to highlighting advances in chemical imaging that could have the greatest impact on critical problems in science and technology, Visualizing Chemistry reviews the current state of chemical imaging technology, identifies promising future developments and their applications, and suggests a research and educational agenda to enable breakthrough improvements.
Download or read book Encyclopedia of Spectroscopy and Spectrometry written by and published by Academic Press. This book was released on 2016-09-22 with total page 3716 pages. Available in PDF, EPUB and Kindle. Book excerpt: This third edition of the Encyclopedia of Spectroscopy and Spectrometry, Three Volume Set provides authoritative and comprehensive coverage of all aspects of spectroscopy and closely related subjects that use the same fundamental principles, including mass spectrometry, imaging techniques and applications. It includes the history, theoretical background, details of instrumentation and technology, and current applications of the key areas of spectroscopy. The new edition will include over 80 new articles across the field. These will complement those from the previous edition, which have been brought up-to-date to reflect the latest trends in the field. Coverage in the third edition includes: Atomic spectroscopy Electronic spectroscopy Fundamentals in spectroscopy High-Energy spectroscopy Magnetic resonance Mass spectrometry Spatially-resolved spectroscopic analysis Vibrational, rotational and Raman spectroscopies The new edition is aimed at professional scientists seeking to familiarize themselves with particular topics quickly and easily. This major reference work continues to be clear and accessible and focus on the fundamental principles, techniques and applications of spectroscopy and spectrometry. Incorporates more than 150 color figures, 5,000 references, and 300 articles for a thorough examination of the field Highlights new research and promotes innovation in applied areas ranging from food science and forensics to biomedicine and health Presents a one-stop resource for quick access to answers and an in-depth examination of topics in the spectroscopy and spectrometry arenas
Download or read book Biomedical Index to PHS supported Research pt A Subject access A H written by and published by . This book was released on 1992 with total page 1064 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Research Awards Index written by and published by . This book was released on 1982 with total page 1212 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Biomedical Index to PHS supported Research written by and published by . This book was released on 1995 with total page 1216 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Comprehensive Inorganic Chemistry II written by and published by Newnes. This book was released on 2013-07-23 with total page 7694 pages. Available in PDF, EPUB and Kindle. Book excerpt: Comprehensive Inorganic Chemistry II, Nine Volume Set reviews and examines topics of relevance to today’s inorganic chemists. Covering more interdisciplinary and high impact areas, Comprehensive Inorganic Chemistry II includes biological inorganic chemistry, solid state chemistry, materials chemistry, and nanoscience. The work is designed to follow on, with a different viewpoint and format, from our 1973 work, Comprehensive Inorganic Chemistry, edited by Bailar, Emeléus, Nyholm, and Trotman-Dickenson, which has received over 2,000 citations. The new work will also complement other recent Elsevier works in this area, Comprehensive Coordination Chemistry and Comprehensive Organometallic Chemistry, to form a trio of works covering the whole of modern inorganic chemistry. Chapters are designed to provide a valuable, long-standing scientific resource for both advanced students new to an area and researchers who need further background or answers to a particular problem on the elements, their compounds, or applications. Chapters are written by teams of leading experts, under the guidance of the Volume Editors and the Editors-in-Chief. The articles are written at a level that allows undergraduate students to understand the material, while providing active researchers with a ready reference resource for information in the field. The chapters will not provide basic data on the elements, which is available from many sources (and the original work), but instead concentrate on applications of the elements and their compounds. Provides a comprehensive review which serves to put many advances in perspective and allows the reader to make connections to related fields, such as: biological inorganic chemistry, materials chemistry, solid state chemistry and nanoscience Inorganic chemistry is rapidly developing, which brings about the need for a reference resource such as this that summarise recent developments and simultaneously provide background information Forms the new definitive source for researchers interested in elements and their applications; completely replacing the highly cited first edition, which published in 1973
Download or read book Comprehensive Biophysics written by and published by Academic Press. This book was released on 2012-04-12 with total page 3533 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biophysics is a rapidly-evolving interdisciplinary science that applies theories and methods of the physical sciences to questions of biology. Biophysics encompasses many disciplines, including physics, chemistry, mathematics, biology, biochemistry, medicine, pharmacology, physiology, and neuroscience, and it is essential that scientists working in these varied fields are able to understand each other's research. Comprehensive Biophysics, Nine Volume Set will help bridge that communication gap. Written by a team of researchers at the forefront of their respective fields, under the guidance of Chief Editor Edward Egelman, Comprehensive Biophysics, Nine Volume Set provides definitive introductions to a broad array of topics, uniting different areas of biophysics research - from the physical techniques for studying macromolecular structure to protein folding, muscle and molecular motors, cell biophysics, bioenergetics and more. The result is this comprehensive scientific resource - a valuable tool both for helping researchers come to grips quickly with material from related biophysics fields outside their areas of expertise, and for reinforcing their existing knowledge. Biophysical research today encompasses many areas of biology. These studies do not necessarily share a unique identifying factor. This work unites the different areas of research and allows users, regardless of their background, to navigate through the most essential concepts with ease, saving them time and vastly improving their understanding The field of biophysics counts several journals that are directly and indirectly concerned with the field. There is no reference work that encompasses the entire field and unites the different areas of research through deep foundational reviews. Comprehensive Biophysics fills this vacuum, being a definitive work on biophysics. It will help users apply context to the diverse journal literature offering, and aid them in identifying areas for further research Chief Editor Edward Egelman (E-I-C, Biophysical Journal) has assembled an impressive, world-class team of Volume Editors and Contributing Authors. Each chapter has been painstakingly reviewed and checked for consistent high quality. The result is an authoritative overview which ties the literature together and provides the user with a reliable background information and citation resource
Download or read book Protein Self Assembly written by Jennifer J. McManus and published by Humana. This book was released on 2020-08-08 with total page 266 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume explores experimental and computational approaches to measuring the most widely studied protein assemblies, including condensed liquid phases, aggregates, and crystals. The chapters in this book are organized into three parts: Part One looks at the techniques used to measure protein-protein interactions and equilibrium protein phases in dilute and concentrated protein solutions; Part Two describes methods to measure kinetics of aggregation and to characterize the assembled state; and Part Three details several different computational approaches that are currently used to help researchers understand protein self-assembly. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Protein Self-Assembly: Methods and Protocols is a valuable resource for researchers who are interested in learning more about this developing field.
Download or read book Encyclopedia of Spectroscopy and Spectrometry A H written by John C. Lindon and published by . This book was released on 2000 with total page 1008 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Encyclopedia provides coverage of current knowledge in the field of spectroscopy and related areas.
