Download or read book Handbook of Tissue Optical Clearing written by Valery V. Tuchin and published by CRC Press. This book was released on 2022-02-04 with total page 682 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biomedical photonics is currently one of the fastest growing fields, connecting research in physics, optics, and electrical engineering coupled with medical and biological applications. It allows for the structural and functional analysis of tissues and cells with resolution and contrast unattainable by any other methods. However, the major challenges of many biophotonics techniques are associated with the need to enhance imaging resolution even further to the sub-cellular level as well as translate them for in vivo studies. The tissue optical clearing method uses immersion of tissues into optical clearing agents (OCAs) that reduces the scattering of tissue and makes tissue more transparent and this method has been successfully used ever since. This book is a self-contained introduction to tissue optical clearing, including the basic principles and in vitro biological applications, from in vitro to in vivo tissue optical clearing methods, and combination of tissue optical clearing and various optical imaging for diagnosis. The chapters cover a wide range of issues related to the field of tissue optical clearing: mechanisms of tissue optical clearing in vitro and in vivo; traditional and innovative optical clearing agents; recent achievements in optical clearing of different tissues (including pathological tissues) and blood for optical imaging diagnosis and therapy. This book provides a comprehensive account of the latest research and possibilities of utilising optical clearing as an instrument for improving the diagnostic effectiveness of modern optical diagnostic methods. The book is addressed to biophysicist researchers, graduate students and postdocs of biomedical specialties, as well as biomedical engineers and physicians interested in the development and application of optical methods in medicine. Key features: The first collective reference to collate all known knowledge on this topic Edited by experts in the field with chapter contributions from subject area specialists Brings together the two main approaches in immersion optical clearing into one cohesive book

Download or read book Deep Imaging in Tissue and Biomedical Materials written by Lingyan Shi and published by CRC Press. This book was released on 2017-03-16 with total page 444 pages. Available in PDF, EPUB and Kindle. Book excerpt: The use of light for probing and imaging biomedical media is promising for the development of safe, noninvasive, and inexpensive clinical imaging modalities with diagnostic ability. The advent of ultrafast lasers has enabled applications of nonlinear optical processes, which allow deeper imaging in biological tissues with higher spatial resolution. This book provides an overview of emerging novel optical imaging techniques, Gaussian beam optics, light scattering, nonlinear optics, and nonlinear optical tomography of tissues and cells. It consists of pioneering works that employ different linear and nonlinear optical imaging techniques for deep tissue imaging, including the new applications of single- and multiphoton excitation fluorescence, Raman scattering, resonance Raman spectroscopy, second harmonic generation, stimulated Raman scattering gain and loss, coherent anti-Stokes Raman spectroscopy, and near-infrared and mid-infrared supercontinuum spectroscopy. The book is a comprehensive reference of emerging deep tissue imaging techniques for researchers and students working in various disciplines.

Download or read book Biomedical Optical Imaging written by James G. Fujimoto and published by Oxford University Press. This book was released on 2009-04-22 with total page 440 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biomedical optical imaging is a rapidly emerging research area with widespread fundamental research and clinical applications. This book gives an overview of biomedical optical imaging with contributions from leading international research groups who have pioneered many of these techniques and applications. A unique research field spanning the microscopic to the macroscopic, biomedical optical imaging allows both structural and functional imaging. Techniques such as confocal and multiphoton microscopy provide cellular level resolution imaging in biological systems. The integration of this technology with exogenous chromophores can selectively enhance contrast for molecular targets as well as supply functional information on processes such as nerve transduction. Novel techniques integrate microscopy with state-of-the-art optics technology, and these include spectral imaging, two photon fluorescence correlation, nonlinear nanoscopy; optical coherence tomography techniques allow functional, dynamic, nanoscale, and cross-sectional visualization. Moving to the macroscopic scale, spectroscopic assessment and imaging methods such as fluorescence and light scattering can provide diagnostics of tissue pathology including neoplastic changes. Techniques using light diffusion and photon migration are a means to explore processes which occur deep inside biological tissues and organs. The integration of these techniques with exogenous probes enables molecular specific sensitivity.

Download or read book Optical Imaging of Biological Tissues written by Jorge Bouza Dominguez and published by . This book was released on 2011 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this thesis, a new time-dependent model for describing light propagation in biological media is proposed. The model is based on the simplified spherical harmonics approximation and is represented by a set of coupled parabolic partial differential equations (TD-pSPN equations). In addition, the model is extended for modeling the time-dependent response of fluorescent agents in biological tissues and the ensuing time-domain propagation of light therein. In a comparison with Monte Carlo simulations, it is shown that the TD-pSPN equations present unique features in its derivation that makes it a more accurate alternative to the diffusion equation (DE). The TD-pSPN model (for orders N > 1) outperforms the DE in the description of the propagation of light in near-nondiffusive media and in all the physical situations where DE fails. Often, only small orders of the SP N approximation are needed to obtain accurate results. A diffuse optical tomography (DOT) algorithm is also implemented based on the TD-pSPN equations as the forward model using constrained optimization methods. The algorithm uses time-dependent (TD) data directly. Such an approach is benefited from both the accuracy of the SPN models and the richness of TD data. In the calculation of the gradient of the objective function, a time-dependent adjoint differentiation method is introduced that reduces computation time. Several numerical experiments are performed for small geometry media with embedded inclusions that mimic small animal imaging. In these experiments, the values of the optical coefficients are varied within realistic bounds that are representative of those found in the range of the near-infrared spectrum, including high absorption values. Single and multi-parameter reconstructions (absorption and diffusion coefficients) are performed. The reconstructed images based on the TD-pSPN equations (N > 1) give better estimates of the optical properties of the media than the DE. On the other hand, crosstalk effects and small artifacts appeared in all the cases (more intense in the DE images). Comparatively, the reconstructed images show a lesser influence of these undesirable 'effects than other approaches found in the literature. The results suggest that the DOT algorithm based on the TD-pSPN model is an accurate alternative to the DE for imaging optical properties of biological media. These results directly benefict the fields of therapeutics and time-domain optical imaging of biological tissues. Particularly, the presented work is a decisive step in the elaboration of an optical scanner for small animal imaging at our lab. Thus, a positive impact in the areas of clinical diagnosis and biomedical research are expected.

Download or read book Biomedical Optics written by Lihong V. Wang and published by John Wiley & Sons. This book was released on 2012-09-26 with total page 378 pages. Available in PDF, EPUB and Kindle. Book excerpt: This entry-level textbook, covering the area of tissue optics, is based on the lecture notes for a graduate course (Bio-optical Imaging) that has been taught six times by the authors at Texas A&M University. After the fundamentals of photon transport in biological tissues are established, various optical imaging techniques for biological tissues are covered. The imaging modalities include ballistic imaging, quasi-ballistic imaging (optical coherence tomography), diffusion imaging, and ultrasound-aided hybrid imaging. The basic physics and engineering of each imaging technique are emphasized. A solutions manual is available for instructors; to obtain a copy please email the editorial department at [email protected].

Download or read book Two Dimensional Angular Domain Optical Imaging in Biological Tissues written by Eldon Ng and published by . This book was released on 2013 with total page 254 pages. Available in PDF, EPUB and Kindle. Book excerpt: Optical imaging is a modality that can detect optical contrast within a biological sample that is not detectable with other conventional imaging techniques. Optical transillumination images of tissue samples are degraded by optical scatter. Angular Domain Imaging (ADI) is an optical imaging technique that filters scattered photons based on the trajectory of the photons. Previous angular filters were limited to one-dimensional arrays greatly limiting the imaging capability of the system We have developed a 2D Angular Filter Array (AFA) that is capable of acquiring two-dimensional projection images of a sample. The AFA was constructed using rapid prototyping techniques. The contrast and the resolution of the AFA were evaluated. The results suggest that a 2D AFA can be used to acquire two-dimensional projection images of a sample with a reduced acquisition time compared to a scanning 1D AFA.

Download or read book Optical Imaging in Human Disease and Biological Research written by Xunbin Wei and published by Springer Nature. This book was released on 2021-05-29 with total page 309 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book introduces readers to the basic principle of optical imaging technologies. Focusing on human disease diagnostics using optical imaging methods, it provides essential information for researchers in various fields and discusses the latest trends in optical imaging. In recent decades, there has been a huge increase in imaging technologies and their applications in human diseases diagnostics, including magnetic resonance imaging, x-ray computed tomography, and nuclear tomographic imaging. This book promotes further developments to extend optical imaging to a wider range of disease diagnostics. It is a valuable resource for researchers and students in the field of biomedical optics, as well as for clinicians.

Download or read book Optical Imaging in Biological Tissues and Tissue Phantoms written by Prashant Shukla and published by LAP Lambert Academic Publishing. This book was released on 2012-04 with total page 164 pages. Available in PDF, EPUB and Kindle. Book excerpt: The early detection and subsequent prevention of cancer have been a challenging task for researchers all over the world. Some of the conventional techniques used for the diagnosis of cancer are mammography, ultrasonography, magnetic resonance imaging (MRI), positron emission tomography (PET) and histopathology. In recent years, optical imaging is being developed as powerful techniques for the early and quick diagnosis of cancer. This book provides the information about polarimetric techniques and their applications in diagnosis of cancer in biological tissues. Polarization gating and Mueller decomposition imaging technique were addressed in the detail with their applications on cervical tissues and mimic of tissues (tissue phantoms). Polarization gated imaging can be used to detect mature tumors in the superficial layers. However, Mueller decomposition images have shown promising results about dysplasia in human cervical tissues. These images can be used to discriminate normal cervical tissue against the dysplastic (pre-cancerous) tissue. These results can be useful for clinical purposes and in the research work for diagnostic tools

Download or read book Translational Multimodality Optical Imaging written by Fred S. Azar and published by Artech House. This book was released on 2008 with total page 431 pages. Available in PDF, EPUB and Kindle. Book excerpt: Supported with 119 illustrations, this milestone work discusses key optical imaging techniques in self-contained chapters; describes the integration of optical imaging techniques with other modalities like MRI, X-ray imaging, and PET imaging; provides a software platform for multimodal integration; presents cutting-edge computational and data processing techniques that ensure rapid, cost-effective, and precise quantification and characterization of the clinical data; covers advances in photodynamic therapy and molecular imaging, and reviews key clinical studies in optical imaging along with regulatory and business issues.

Download or read book Optical Imaging Through Biological Tissues written by Robert Paul Francis and published by . This book was released on 2015 with total page 162 pages. Available in PDF, EPUB and Kindle. Book excerpt: Optical light propagation in biological tissues guides medical instrument design for diagnosis and treatment of disease. While many instruments are already commercially available and approved for patient use, it is important to continue to push the envelope of new technology development by analyzing new techniques and phenomena of light-tissue interaction. Technology development is necessary for endoscopic surgery because there are still occurrences of injury due to inadequate imaging of anatomy. To aid in future endoscopic instrument design, this dissertation explores the propagation of 1430-1450 nm light in visceral fat, gall bladder, and water as it relates to endoscopic gastroenterological surgery. Technology development is also necessary for traumatic brain injury (TBI) because diagnosis outside of hospitals is currently inadequate to catch all occurrences of the injury. To guide future instrument development, this dissertation explores the propagation of 750-1050 nm light in cranium, brain, and blood as it relates to detection and monitoring of hematomas associated with TBI. The dissertation is organized into two parts of three chapters each: (Part I) Diffuse Optical Transmission through Visceral Fat Tissue, and (Part II) Diffuse Optical Reflectance for Traumatic Brain Injury. Within Part I, the chapters are: (1) Introduction and Modeling of Transmission, (2) Instrumentation Setup and Experimentation Results, and (3) Exploration of Heterogeneities. Within Part II, the chapters are: (4) Introduction and Modeling of Reflectance, (5) Simulation Results and Applications to TBI, and (6) Modeling Optical Probe Efficiency through Hair.

Download or read book Biomedical Optical Imaging Technologies written by Rongguang Liang and published by Springer Science & Business Media. This book was released on 2012-09-21 with total page 391 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides an introduction to design of biomedical optical imaging technologies and their applications. The main topics include: fluorescence imaging, confocal imaging, micro-endoscope, polarization imaging, hyperspectral imaging, OCT imaging, multimodal imaging and spectroscopic systems. Each chapter is written by the world leaders of the respective fields, and will cover: principles and limitations of optical imaging technology, system design and practical implementation for one or two specific applications, including design guidelines, system configuration, optical design, component requirements and selection, system optimization and design examples, recent advances and applications in biomedical researches and clinical imaging. This book serves as a reference for students and researchers in optics and biomedical engineering.

Download or read book Advancements in Angular Domain Optical Imaging in Biological Tissues written by Fartash Vasefi and published by . This book was released on 2010 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Angular Domain Imaging (ADI) is a technique for performing optical imaging through highly scattering media. The basis for the technique is the micro-machined Angular Filter Array (AFA), which provides a parallel collection of micro-tunnels that accept ballistic/quasi-ballistic image-bearing photons and reject multiply scattered photons that result in image-destroying background noise. At high scattering levels, ADI image contrast declines due to the non-uniform scattered background light within the acceptance angle of the AFA. In this thesis, I developed multiple methodologies to correct for this problem and enhance ADI image contrast at higher scattering levels. These methodologies included combining ADI with time gating, polarization gating and employing image processing to estimate the background scattered light and use this information to enhance ADI image contrast and resolution. Furthermore, I conducted a comprehensive experimental investigation on a new AFA geometry designed to reduce the reflections within the micro-tunnels to reduce the unwanted background noise caused by multiply scattered photons. Building on previous studies with ADI in a trans-illumination configuration, I demonstrated that ADI could also be used to capture information-carrying photons from diffuse light back-reflected from tissue, where illumination was from the same side as the AFA. This mode of operation will enable applications of ADI where trans-illumination of samples is not possible. I also developed a tomographic ADI modality that rotated the sample and compiled ADI shadowgrams at each angle into a sinogram, followed by reconstruction of a transverse image with depth information. I also exploited the collimation detection capabilities of the AFA to extract photons emitted by a fluorophore embedded at depth within a turbid medium. The fluorescent imaging system using AFA offered higher resolution and contrast compared to a conventional lens and lens-pinhole fluorescent detection system in both in vitro and animal tests. Optical imaging with an AFA does not depend on coherence of the light source or the wavelength of light. Therefore, it is a promising candidate for multispectral/hyperspectral imaging to localize absorption and/or fluorescence in tissue and may have particular importance in cancer optical imaging.

Download or read book Optical Methods and Instrumentation in Brain Imaging and Therapy written by Steen J. Madsen and published by Springer Science & Business Media. This book was released on 2012-11-13 with total page 284 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a comprehensive up-to-date review of optical approaches used in brain imaging and therapy. It covers a variety of imaging approaches including diffuse optical imaging, laser speckle imaging, photoacoustic imaging and optical coherence tomography. A number of laser-based therapeutic techniques are reviewed, including photodynamic therapy, fluorescence guided resection and photothermal therapy. Fundamental principles and instrumentation are discussed for each imaging and therapeutic approach.

Download or read book Optical Techniques in Regenerative Medicine written by Stephen P. Morgan and published by Taylor & Francis. This book was released on 2016-04-19 with total page 445 pages. Available in PDF, EPUB and Kindle. Book excerpt: In regenerative medicine, tissue engineers largely rely on destructive and time-consuming techniques that do not allow in situ and spatial monitoring of tissue growth. Furthermore, once the therapy is implanted in the patient, clinicians are often unable to monitor what is happening in the body. To tackle these barriers, optical techniques have bee

Download or read book Advanced Biophotonics written by Ruikang K. Wang and published by Taylor & Francis. This book was released on 2016-04-19 with total page 726 pages. Available in PDF, EPUB and Kindle. Book excerpt: Despite a number of books on biophotonics imaging for medical diagnostics and therapy, the field still lacks a comprehensive imaging book that describes state-of-the-art biophotonics imaging approaches intensively developed in recent years. Addressing this shortfall, Advanced Biophotonics: Tissue Optical Sectioning presents contemporary methods and

Download or read book Optical Imaging Techniques in Cell Biology written by Guy Cox and published by CRC Press. This book was released on 2006-11-06 with total page 304 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the word microscopy was coined in 1656, the evolution of the instrument has had a long and convoluted history. Plagued with problems of chromatic aberration, spherical aberration, and challenges with illumination and resolution, the microscope's technical progression happened in a series of fits and starts until the late 19th century. After E

Download or read book Adaptive Optics for Biological Imaging written by Joel A Kubby and published by CRC Press. This book was released on 2013-04-26 with total page 390 pages. Available in PDF, EPUB and Kindle. Book excerpt: Adaptive Optics for Biological Imaging brings together groundbreaking research on the use of adaptive optics for biological imaging. The book builds on prior work in astronomy and vision science. Featuring contributions by leaders in this emerging field, it takes an interdisciplinary approach that makes the subject accessible to nonspecialists who want to use adaptive optics techniques in their own work in biology and bioengineering. Organized into three parts, the book covers principles, methods, and applications of adaptive optics for biological imaging, providing the reader with the following benefits: Gives a general overview of applied optics, including definitions and vocabulary, to lay a foundation for clearer communication across disciplines Explains what kinds of optical aberrations arise in imaging through various biological tissues, and what technology can be used to correct for these aberrations Explores research done with a variety of biological samples and imaging instruments, including wide-field, confocal, and two-photon microscopes Discusses both indirect wavefront sensing, which uses an iterative approach, and direct wavefront sensing, which uses a parallel approach Since the sample is an integral part of the optical system in biological imaging, the field will benefit from participation by biologists and biomedical researchers with expertise in applied optics. This book helps lower the barriers to entry for these researchers. It also guides readers in selecting the approach that works best for their own applications.