Download or read book On fiber Derivatization with Solid Phase Microextraction Coupled to Capillary Electrophoresis with Laser Induced Fluorescence Detection for Chemical Analysis of the Content of Small Objects written by Angelina So and published by . This book was released on 2001 with total page 158 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Solid Phase Microextraction Coupled to Capillary Electrophoresis with Axially Illuminated Laser Induced Fluorescence Imaging Detection written by Tiemin Huang and published by . This book was released on 2000 with total page 180 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Handbook of Solid Phase Microextraction written by Janusz Pawliszyn and published by Elsevier. This book was released on 2011-12-01 with total page 497 pages. Available in PDF, EPUB and Kindle. Book excerpt: The relatively new technique of solid phase microextraction (SPME) is an important tool to prepare samples both in the lab and on-site. SPME is a "green" technology because it eliminates organic solvents from analytical laboratory and can be used in environmental, food and fragrance, and forensic and drug analysis. This handbook offers a thorough background of the theory and practical implementation of SPME. SPME protocols are presented outlining each stage of the method and providing useful tips and potential pitfalls. In addition, devices and fiber coatings, automated SPME systems, SPME method development, and In Vivo applications are discussed. This handbook is essential for its discussion of the latest SPME developments as well as its in depth information on the history, theory, and practical application of the method. Practical application of Solid Phase Microextraction methods including detailed steps Provides history of extraction methods to better understand the process Suitable for all levels, from beginning student to experienced practitioner

Download or read book Solid Phase Microextraction written by Gangfeng Ouyang and published by Springer. This book was released on 2016-11-24 with total page 313 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book offers comprehensive information on the developments and applications of the solid phase microextraction (SPME) technique. The first part of the book briefly introduces readers to the fundamentals of SPME, while subsequent sections describe the applications of SPME technique in detail, including environmental analysis (air, water, soil/sediments), food analysis (volatile/nonvolatile compounds), and bioanalysis (plants, animal tissues, body fluids). The advantages and future challenges of the SPME technique are also discussed. Including recent research advances and further developments of SPME, the book offers a practical reference guide and a valuable resource for researchers and users of SPME techniques. The target audience includes analytical chemists, environmental scientists, biological scientists, material scientists, and analysts, as well as students at universities/institutes in related fields. Dr. Gangfeng Ouyang is a Professor at the School of Chemistry and Chemical Engineering, Sun Yat-sen University, China. Dr. Ruifen Jiang is an Associate Professor at the School of Environment, Jinan University, China.

Download or read book Site specific Fluorescent Derivatization of Peptides for Characterization by Capillary Electrophoresis with Laser induced Fluorescence Detection written by Sara Jane Sudmeier and published by . This book was released on 1998 with total page 230 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Applications of Capillary Electrophoresis and Laser induced Fluorescence Detection to the Analysis of Trace Species written by Qifeng Xue and published by . This book was released on 1995 with total page 258 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Applications of Capillary Electrophoresis and Laser induced Fluorescence Detection to the Analysis of Trace Species written by and published by . This book was released on 1995 with total page 52 pages. Available in PDF, EPUB and Kindle. Book excerpt: This Ph. D. Thesis describes several separation and detection schemes for the analysis of small volume and amount of samples, such as intracellular components and single enzymes developed during research. Indirect Laser-induced fluorescence detection and capillary electrophoresis were used to quantify lactate and pyruvate in single red blood cells. The assay of specific enzyme activities was achieved by monitoring the highly fluorescent enzymatic reaction product, NADH. LDH activity was found not to be a unique marker for diagnosis of leukemia. Reactions of single LDH-1 molecules were investigated by monitoring the reaction product with LIF detection.

Download or read book Fluorescence detection techniques in liquid chromatography written by Lars Johnson and published by . This book was released on 1982 with total page 150 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Fluorescent Derivatization of Arginine and Carbonyl containing Analytes for Analysis by Capillary Electrophoresis with Laser induced Fluorescence Detection written by and published by . This book was released on 2000 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Coupling of Solid phase Extraction and Derivatization with Capillary Electrophoresis for Peptide Analysis written by Frans Willem Alexander Tempels and published by . This book was released on 2007 with total page 173 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book High throughput Analysis with 96 capillary Array Electrophoresis and Integrated Sample Preparation for DNA Sequencing Based on Laser Induced Fluorescence Detection written by Gang Xue and published by . This book was released on 2001 with total page 266 pages. Available in PDF, EPUB and Kindle. Book excerpt: The purpose of this research was to improve the fluorescence detection for the multiplexed capillary array electrophoresis, extend its use beyond the genomic analysis, and to develop an integrated micro-sample preparation system for high-throughput DNA sequencing. We first demonstrated multiplexed capillary zone electrophoresis (CZE) and micellar electrokinetic chromatography (MEKC) separations in a 96-capillary array system with laser-induced fluorescence detection. Migration times of four kinds of fluoresceins and six polyaromatic hydrocarbons (PAHs) are normalized to one of the capillaries using two internal standards. The relative standard deviations (RSD) after normalization are 0.6-1.4% for the fluoresceins and 0.1-1.5% for the PAHs. Quantitative calibration of the separations based on peak areas is also performed, again with substantial improvement over the raw data. This opens up the possibility of performing massively parallel separations for high-throughput chemical analysis for process monitoring, combinatorial synthesis, and clinical diagnosis. We further improved the fluorescence detection by step laser scanning. A computer-controlled galvanometer scanner is adapted for scanning a focused laser beam across a 96-capillary array for laser-induced fluorescence detection. The signal at a single photomultiplier tube is temporally sorted to distinguish among the capillaries. The limit of detection for fluorescein is 3 x 10−11M (S/N=3) for 5-mW of total laser power scanned at 4 Hz. The observed cross-talk among capillaries is 0.2%. A simple two-dimensional laser scanning scheme was also proposed with slight modification. 488 nm and 514 nm laser lines from a single Ar laser were alternatively scanned through the array to achieve two-color basecalling for successful high-throughput DNA sequencing. Finally, we addressed the sample preparation for the high-throughput DNA sequencing. An integrated system of nanoreactor for cycle-sequencing reaction coupled with online CZE purification and CGE DNA sequencing is presented. Less than 100 nl of cycle sequencing premixed reagent solution was hydrodynamically injected into a fused-silica capillary (75 [mu]m i.d.) inside a homemade microthermocycler for successful sample preparation, online purification, sequencing separation, and basecalling. The whole system is readily adaptable to automated multiplex operation for DNA sequencing or PCR analysis.

Download or read book Two wavelength Laser Induced Fluorescence Detection for Capillary Electrophoresis of Proteins and Peptides written by Guido Fridolin Verbeck and published by . This book was released on 1999 with total page 136 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book High Throughput Analysis With 96 Capillary Array Electrophoresis and Integrated Sample Preparation for DNA Sequencing Based on Laser Induced Fluorescence Detection written by and published by . This book was released on 2001 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The purpose of this research was to improve the fluorescence detection for the multiplexed capillary array electrophoresis, extend its use beyond the genomic analysis, and to develop an integrated micro-sample preparation system for high-throughput DNA sequencing. The authors first demonstrated multiplexed capillary zone electrophoresis (CZE) and micellar electrokinetic chromatography (MEKC) separations in a 96-capillary array system with laser-induced fluorescence detection. Migration times of four kinds of fluoresceins and six polyaromatic hydrocarbons (PAHs) are normalized to one of the capillaries using two internal standards. The relative standard deviations (RSD) after normalization are 0.6-1.4% for the fluoresceins and 0.1-1.5% for the PAHs. Quantitative calibration of the separations based on peak areas is also performed, again with substantial improvement over the raw data. This opens up the possibility of performing massively parallel separations for high-throughput chemical analysis for process monitoring, combinatorial synthesis, and clinical diagnosis. The authors further improved the fluorescence detection by step laser scanning. A computer-controlled galvanometer scanner is adapted for scanning a focused laser beam across a 96-capillary array for laser-induced fluorescence detection. The signal at a single photomultiplier tube is temporally sorted to distinguish among the capillaries. The limit of detection for fluorescein is 3 x 10−11 M (S/N = 3) for 5-mW of total laser power scanned at 4 Hz. The observed cross-talk among capillaries is 0.2%. Advantages include the efficient utilization of light due to the high duty-cycle of step scan, good detection performance due to the reduction of stray light, ruggedness due to the small mass of the galvanometer mirror, low cost due to the simplicity of components, and flexibility due to the independent paths for excitation and emission.

Download or read book Capillary Gel Electrophoresis written by Andras Guttman and published by Newnes. This book was released on 2021-12-04 with total page 391 pages. Available in PDF, EPUB and Kindle. Book excerpt: Capillary Gel Electrophoresis and Related Microseparation Techniques covers all theoretical and practical aspects of capillary gel electrophoresis. It also provides an excellent overview of the key application areas of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods. It not only gives readers a better understanding of how to utilize this technology, but also provides insights into how to determine which method will provide the best technical solutions to particular problems. This book can also serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses. Covers all theoretical and practical aspects of capillary gel electrophoresis Excellent overview of the key applications of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods Teaches readers how to use the technology and select methods that are ideal for fundamental problems Can serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses

Download or read book Open Tubular Capillary Electrochromatography laser Induced Fluorescence for the Separation and Detection of Proteins and Amino Acids written by Kellie M. Davis and published by . This book was released on 2007 with total page 69 pages. Available in PDF, EPUB and Kindle. Book excerpt: "With its high efficiency and reproducibility, open tubular capillary electrochromatography (OTCEC) is beginning to prove itself as a promising method for the separation of proteins, peptides and pharmaceuticals. OTCEC, when coupled to an absorbance-based detector, suffers from poor sensitivity and high concentration limits of detection. However, laser induced fluorescence coupled to CEC has shown picomolar limits of detections for some compounds.2 This thesis describes the coupling of OTCEC to a laser induced fluorescence (LIF) detector to optimize both the separation and detection aspects of protein and amino acid analysis. OTCEC capillaries were etched and silanized, and the 4,4' cyanopentoxy biphenyl phase was attached through a radical-initiated process. Such biphenyl phases have shown unique dependence on temperature and mobile phase composition and demonstrated higher selectivity for certain compounds3 than bare silica capillaries. Proteins and amino acids were modified using pre-column derivatization formats with two different fluorescein-based dyes (fluorescein isothiocyanate, FITC) and Atto-tag FQ (3-(2-furoyl)quinoline-2-carboxaldehyde). These conditions were varied to attempt consistent labeling conditions with varying protein size. Optimizations of the analyses were achieved by modifying conditions such as background electrolyte, pH, voltage, and percent organic modifiers. These modified conditions were used to achieve the highest resolution on protein and amino acid derivatives. Results determined that high sensitivity and efficiency are achieved when LIF detection is coupled to cyano pentoxy biphenyl coated capillaries using optimal conditions. These conditions include 1) the use of Atto-tag FQ derivatization and 2) an 80:20 acetonitrile: 50 mM phosphate buffer (pH 2.00) background electrolyte with a positive electrical current applied."--Abstract from author supplied metadata.

Download or read book Integrated Laser Induced Fluorescence Detection for Parallelizable Microfluidic Single Cell Analysis written by Debbie Lo and published by . This book was released on 2017 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microfluidic devices employing on-chip capillary electrophoresis (ÂľCE) have the potential to analyze single cells at high throughput while demanding less sample volume. However, to date, detecting the lower limit of the biological dynamic range involves coupling the fluorescence into confocal set-ups using externally cooled photomultiplier tubes (PMTs) which inhibit parallelization of the analysis. The integration of photodetector elements directly on-chip is desirable, but thus far, demonstrated systems are unable to detect targets without increasing target concentration by chemical techniques such as polyermase chain reaction (PCR). To address these demands, this work will investigate a laser-induced fluorescence (LIF) detection scheme using a high-index micro-ball lens embedded in a carbon-blackened polydimethylsiloxane (PDMS) panchromatic filter. The lens-filter layer enables simultaneous excitation rejection and fluorescence enhancement to couple fluorescence from a microfluidic channel to a surface detector. Furthermore, a method is proposed to optimize signal-to-noise ratio (SNR) in these higher dark current environments towards the goal of multiplexable and parallelizable integrated LIF detection.

Download or read book Development of High Sensitivity Capillary Electrophoresis Fluorescence System for the Detection of Biological Molecules written by and published by . This book was released on 2000 with total page 31 pages. Available in PDF, EPUB and Kindle. Book excerpt: One class of chemical & biological weapons consists of toxic proteins & peptides produced by biological organisms, or mid-spectrum agents. The research described in this report considered the analysis & detection of these mid-spectrum agents based on chemical methods, specifically via the use of capillary electrophoresis (CE) coupled with laser induced fluorescence (LIF). The report first presents a brief literature review and then highlights results of three research themes. Research papers detailing the experiments & results of these themes are appended. The first paper reports a method for assay of proteins at picomolar concentrations by CE, precolumn labelling, sub-micellar separation, and LIF detection. The second describes the use of sieving media for the separation of labeled proteins. The last paper addresses on-column labeling & analysis of proteins with a fluorogenic reagent. Overall results of the methods developed are compared to those achieved with ultraviolet absorbance detection.