Download or read book Oligonucleotide Synthesis Using the Hydrogenphosphonate Approach written by Tor Regberg and published by . This book was released on 1989 with total page 42 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Oligonucleotide Synthesis written by Piet Herdewijn and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 436 pages. Available in PDF, EPUB and Kindle. Book excerpt: A collection of powerful new techniques for oligonucleotide synthesis and for the use of modified oligonucleotides in biotechnology. Among the protocol highlights are a novel two-step process that yields a high purity, less costly, DNA, the synthesis of phosphorothioates using new sulfur transfer agents, the synthesis of LNA, peptide conjugation methods to improve cellular delivery and cell-specific targeting, and triple helix formation. The applications include using molecular beacons to monitor the PCR amplification process, nuclease footprinting to study the sequence-selective binding of small molecules of DNA, nucleic acid libraries, and the use of small interference RNA (siRNA) as an inhibitor of gene expression.
Download or read book Protocols for Oligonucleotides and Analogs written by Sudhir Agrawal and published by Springer Science & Business Media. This book was released on 1993-08-31 with total page 503 pages. Available in PDF, EPUB and Kindle. Book excerpt: When first conceived, not only was the aim of Protocols for Oligo nucleotides and Analogs to provide wide coverage of the ohgonuc- otide chemistry field for readers who are well versed within the field, but also to give investigators just entering into the field a new perspective. The very first book on this topic was edited and published by Michael Gait in 1984, in whose laboratory I encountered the newer aspects of oligonucleotide chemistry. Since then, oligonucleotide research has developed to such an extent that its uses extend far beyond basic studies, and now find wide application throughout clinical science as well. Until recently, the major application of oligonucleotides has been in the area of DNA-based diagnostic and "antisense oligonucleotid- based therapeutic approaches. However, oligonucleotides are now also being used as therapeutic agents and are thus frequently found in clinical trials in humans. Synthesis of unmodified oligonucleotides using automated synthe sizers has become a common practice in numerous laboratories. How ever, improvements on the existing techniques and the introduction of ever newer methods for oligonucleotide synthesis is constantly driving ahead in the leading research laboratories. And several new oligonucle otide analogs have been synthesized and studied for their individual prop erties in recent years. The present volume strives to bring the readers the most up-to-date information on the newest aspects of synthesis of oligo nucleotides and their analogs. A separate volume covers synthesis of oligonucleotide conjugates, along with most of the analytical techniques presently used for analysis of oligonucleotides.
Download or read book Oligonucleotide Synthesis written by Michael J. Gait and published by . This book was released on 1984 with total page 242 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Synthesis of Therapeutic Oligonucleotides written by Satoshi Obika and published by Springer. This book was released on 2018-12-08 with total page 280 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents the latest knowledge on a broad range of topics relating to the synthesis of natural and artificial oligonucleotides with therapeutic potential. Nucleic acid-based therapeutics are attracting much attention, and numerous therapeutic oligonucleotides, such as antisense oligonucleotides, siRNAs, splice-switching oligonucleotides, and nucleic acid aptamers, are being evaluated in clinical trials for the treatment of a variety of diseases. Synthesis of Therapeutic Oligonucleotides covers a broad range of topics in the field that are of high relevance to researchers, including the synthesis of natural and chemically modified oligonucleotides, the development of novel nucleic acid analogs, industrial scale synthesis and purification of oligonucleotides, and important aspects of chemistry, manufacturing, and controls (CMC). The aim is to provide new insights and inspire fresh ideas in nucleic acid chemistry that may ultimately lead to novel concepts and techniques and the discovery of more effective nucleic acid drugs. The book will be of high value for both established researchers in the field and students intending to specialize in nucleic acid chemistry research.
Download or read book The Hyman Method written by Edward David Hyman and published by . This book was released on 1995 with total page 228 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Oligonucleotides and Analogues written by Fritz Eckstein and published by Oxford University Press, USA. This book was released on 1991 with total page 346 pages. Available in PDF, EPUB and Kindle. Book excerpt: Interest in oligonucleotide synthesis has expanded dramatically since the publication of M. Gait's book in this series in 1984. The process has been automated, new reagents have been developed, and the range of uses for the compounds produced has increased and continues to grow. This volume provides practical guidance on oligonucleotide synthesis and methods for introducing modifications into these molecules. State- of-the-art techniques for automated synthesis are covered in two chapters on the production of oligodeoxynucleotides and oligoribonucleotides. Synthesis of modified oligodeoxynucleotides is also detailed, including modification of the phosphate backbone to produce phosphorothioates, phosphorodithiolates and methyl phosphonates, all of which are of considerable importance due to thier potential therapeutic applications. Other chapters decsribe production of sugar-modified ilgodeoxynucleotides and the attachment of various reported groups; these techniques are useful to those interested in non-radioactive probes for hybridization and for the study of DNA-DNA and DNA protein interactions. This book will be of interest to academic and industrial researchers, enabling both chemists and non-chemists to synthesize oligonucleotides and analogues for a wide variety of experimental purposes.
Download or read book A triester approach to oligonucleotide synthesis written by Kelvin Kenneth Ogilvie and published by . This book was released on 1968 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Studies in Oligonucleotide Synthesis A General Approach to the Synthesis of Monaalkyl Phosphates written by J. C. M. Stewart and published by . This book was released on 1969 with total page 148 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Studies Aimed at Improving the H phosphonate Approach for Solid Phase Oligonucleotide Synthesis written by Susannah Sigurdsson and published by . This book was released on 2002 with total page 53 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book New Strategies in Oligonucleotide Synthesis written by Sophie Heidenhain and published by . This book was released on 1999 with total page 162 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Chemistry of Nucleic Acids written by Harri Lönnberg and published by Walter de Gruyter GmbH & Co KG. This book was released on 2024-09-23 with total page 410 pages. Available in PDF, EPUB and Kindle. Book excerpt: Life in all its forms is based on nucleic acids which store and transfer genetic information. The book addresses main aspects of synthesis, hydrolytic stability and solution equilibria of nucleosides, nucleotides and oligonucleotides, as well as synthesis of their structural analogs that are of interest in chemotherapy. In addition, recent achievements in chemistry of catalytic nucleic acids, development of oligonucleotide based drugs and novel strategies for their targeting and delivery are discussed. The central theme always is the correlation of structure and function.
Download or read book Nucleoside H phosphonates written by Roger Strömberg and published by . This book was released on 1987 with total page 54 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Handbook of Organophosphorus Chemistry written by Robert Engel and published by CRC Press. This book was released on 1992-06-19 with total page 896 pages. Available in PDF, EPUB and Kindle. Book excerpt: This practical work summarizes the development of organophosphorus chemistry in topical areas and details the discipline's current state - providing applications and experimental procedures throughout.;Written by 18 leading authorities in the field, the Handbook of Organophosphorus Chemistry: examines advances in the mechanistic understanding of th
Download or read book Advancements in the Synthesis of Oligoribonucleotides on Solid Support and in Solution written by Matthew Hassler and published by . This book was released on 2013 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: "This thesis is focused on the investigation of synthetic approaches for the preparation of oligonucleotides in solution. We describe our efforts to produce these valuable molecules in a more cost effective manner relative to the current industrial standards employing solid supported synthesis. We demonstrate that an ionic tag supported synthesis technique can be successfully applied to oligonucleotide synthesis using a phosphonium-based ionic tag, using simple precipitation and phase separation methods without the need for chromatography to obtain reasonably high product purity at each step. We also explore the optimization of the technique as it is moved to the gram scale and investigate the use of dimer and trimer blocks in the assembly of RNA. This strategy significantly reduced the total number of steps required in the synthesis of a target RNA sequence, provided more material, and simplified separation of the product from shorter failure sequences. The procedure was illustrated by the synthesis of rUpU, rApA, and rUpUpU phosphoramidite blocks and their use in the synthesis of 2-19 nt oligoribonucleotides both in solution and on solid phase. The building blocks utilized a 2ʹ-O-triisopropylsilyl (TIPS) protecting group for the 3ʹ-termini instead of the standard 2ʹ-O-tert-butyldimethylsilyl (TBDMS) protection, in order to eliminate silyl migration during synthesis of dimer and trimer phosphoramidites.The synthesis and use of two novel, orthogonally cleavable linkers for solid and soluble supported synthesis are also described. The linkers are derived from the well known levulinyl and 3ʹ-nitrophenylpropyloxycarbonyl (NPPOC) protecting groups, and expand utility to our methods by providing ready access to dimer and trimer phosphoramidite blocks. As the orthogonal linkers are removed under mild conditions, they will likely find use in the post-synthesis modification of oligonucleotides, the synthesis of oligonucleotides with base sensitive modifications, and siRNA or antisense pro-drugs. " --
Download or read book Oligonucleotide Synthesis written by Steven Anthony Colbert and published by . This book was released on 1995 with total page 272 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Phosphorus Chemistry II written by Jean-Luc Montchamp and published by Springer. This book was released on 2015-03-06 with total page 283 pages. Available in PDF, EPUB and Kindle. Book excerpt: Each review within the volume critically surveys one aspect of that topic and places it within the context of the volume as a whole. The most significant developments of the last 5 to 10 years are presented using selected examples to illustrate the principles discussed. The coverage is not intended to be an exhaustive summary of the field or include large quantities of data, but should rather be conceptual, concentrating on the methodological thinking that will allow the non-specialist reader to understand the information presented. Contributions also offer an outlook on potential future developments in the field.
