Download or read book Nucleic Acid Hybridization written by M.L.M. Anderson and published by Garland Science. This book was released on 2020-08-26 with total page 279 pages. Available in PDF, EPUB and Kindle. Book excerpt: First published in 1998. This book is targeted at the beginner who has little or no knowledge of the principles or practice of hybridization. This text concentrates on solution and filter hybridization with a final chapter on current developments which includes DNA chips and advances in probe design.
Download or read book Nucleic Acid Hybridization written by M.L.M. Anderson and published by Garland Science. This book was released on 2020-08-26 with total page 257 pages. Available in PDF, EPUB and Kindle. Book excerpt: First published in 1998. This book is targeted at the beginner who has little or no knowledge of the principles or practice of hybridization. This text concentrates on solution and filter hybridization with a final chapter on current developments which includes DNA chips and advances in probe design.
Download or read book Nucleic Acid Hybridization in the Study of Cell Differentiation written by H. Ursprung and published by Springer Science & Business Media. This book was released on 2013-06-05 with total page 83 pages. Available in PDF, EPUB and Kindle. Book excerpt: The informational content of cells is encoded in the nucleotide sequences of their DNA. The restrictions on base pairing- A pairing with T (U), and G pairing with C - in nature assures the fidelity of replication of DNA in cell division, and of transcription. In the test tube, these restrictions can be exploited for ascertaining similarities and dissimilarities of nucleic acids of varying origin by measuring the kinetics of reassociation of polynucleotides to double-stranded molecules in DNA DNA renaturation or RNA-DNA hybridization experiments, and by determining the thermal stability and other physical-chemical properties of the resulting hybrid molecules. This method has enormous potential for developmental biology. It offers a more direct approach to the ever-present question of the genetic identity of different cell types in an individual organism, and a more direct test of the hypothesis of diffe rential gene function. It offers the possibility of localizing genes on chromosomes without the use of Mendelian genetics. It is an indispensable tool in the isolation, purification, and characterization of genes. This volume brings together six articles by investigators actively working on various aspects of developmental biology who use nucleic acid hybridization as a tool in their research. Sound in theory, the method is in a honing phase as regards the technical detail. This is expressed in the hesitation with which some of the conclusions are rightly drawn.
Download or read book Basic Techniques in Molecular Biology written by Stefan Surzycki and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 442 pages. Available in PDF, EPUB and Kindle. Book excerpt: This laboratory manual gives a thorough introduction to basic techniques. It is the result of practical experience, with each protocol having been used extensively in undergraduate courses or tested in the authors laboratory. In addition to detailed protocols and practical notes, each technique includes an overview of its general importance, the time and expense involved in its application and a description of the theoretical mechanisms of each step. This enables users to design their own modifications or to adapt the method to different systems. Surzycki has been holding undergraduate courses and workshops for many years, during which time he has extensively modified and refined the techniques described here.
Download or read book Nucleic Acid Hybridization written by P. M. Gilmartin and published by Wiley-Interscience. This book was released on 1996-03-19 with total page 166 pages. Available in PDF, EPUB and Kindle. Book excerpt: This quick reference guide covers the fundamental techniques and practical tips needed to undertake a variety of nucleic acid hybridization methods.
Download or read book Hybridization with Nucleic Acid Probes Part II written by P. Tijssen and published by Elsevier. This book was released on 1993-10-21 with total page 361 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent breakthroughs in recombinant DNA technology and the availability of sophisticated equipment accessible to almost any laboratory, have contributed to the development and perfection of powerful hybridization tools. Recently, nucleic acid hybridization has not only become a cornerstone in molecular biology research but also a powerful supplement to other diagnostic tools.These diagnostic methods are set out in a logical and clear two-part volume in this now famous Laboratory Techniques series. The volume is divided into theory and preparation (Part I), and probe labelling and hybridization techniques (Part II). Both parts are worthy additions to this series, designed for easy access of information on the laboratory bench.
Download or read book Nucleic Acid Hybridization written by J. Ross and published by . This book was released on 1998-03-11 with total page 188 pages. Available in PDF, EPUB and Kindle. Book excerpt: This handy laboratory manual allows quick and easy access to the most important techniques in molecular hybridization. It briefly describes each technique, explaining the advantages and disadvantages and avoiding unnecessary detail. It also explains why certain techniques work (or fail to work).
Download or read book Nucleic Acid Hybridization in the Study of Cell Differentiation written by Heinrich Ursprung and published by . This book was released on 1972 with total page 76 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book New developments in nucleic acid hybridization written by Ann-Christine Syvänen and published by . This book was released on 1986 with total page 11 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Essentials of Medical Biochemistry written by Chung Eun Ha and published by Academic Press. This book was released on 2011-01-28 with total page 600 pages. Available in PDF, EPUB and Kindle. Book excerpt: Expert biochemist N.V. Bhagavan's new work condenses his successful Medical Biochemistry texts along with numerous case studies, to act as an extensive review and reference guide for both students and experts alike. The research-driven content includes four-color illustrations throughout to develop an understanding of the events and processes that are occurring at both the molecular and macrolecular levels of physiologic regulation, clinical effects, and interactions. Using thorough introductions, end of chapter reviews, fact-filled tables, and related multiple-choice questions, Bhagavan provides the reader with the most condensed yet detailed biochemistry overview available. More than a quick survey, this comprehensive text includes USMLE sample exams from Bhagavan himself, a previous coauthor. - Clinical focus emphasizing relevant physiologic and pathophysiologic biochemical concepts - Interactive multiple-choice questions to prep for USMLE exams - Clinical case studies for understanding basic science, diagnosis, and treatment of human diseases - Instructional overview figures, flowcharts, and tables to enhance understanding
Download or read book Landmark Experiments in Molecular Biology written by Michael Fry and published by Academic Press. This book was released on 2016-06-10 with total page 572 pages. Available in PDF, EPUB and Kindle. Book excerpt: Landmark Experiments in Molecular Biology critically considers breakthrough experiments that have constituted major turning points in the birth and evolution of molecular biology. These experiments laid the foundations to molecular biology by uncovering the major players in the machinery of inheritance and biological information handling such as DNA, RNA, ribosomes, and proteins. Landmark Experiments in Molecular Biology combines an historical survey of the development of ideas, theories, and profiles of leading scientists with detailed scientific and technical analysis. Includes detailed analysis of classically designed and executed experiments Incorporates technical and scientific analysis along with historical background for a robust understanding of molecular biology discoveries Provides critical analysis of the history of molecular biology to inform the future of scientific discovery Examines the machinery of inheritance and biological information handling
Download or read book Nucleic Acids Hybridization written by Anton Buzdin and published by Springer Science & Business Media. This book was released on 2007-06-07 with total page 328 pages. Available in PDF, EPUB and Kindle. Book excerpt: Several approaches, such as microarray hybridization, have become extremely popular tools for specialists in biochemistry and biomedicine, while the potential of many other advantageous techniques seems to be underestimated. Written by an international team of authors, this book details the current state-of-art in hybridization techniques. Coverage includes experimental protocols along with comprehensive and detailed method descriptions.
Download or read book Techniques in Molecular Medicine written by Friedhelm Hildebrandt and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 394 pages. Available in PDF, EPUB and Kindle. Book excerpt: This manual not only provides reliable, up-to-date protocols for lab use but also the theoretical background of molecular biology, allowing users to better understand the principles underlying these techniques. It covers a wide range of methods, including the purification of nucleic acids, enzymatic modification of DNA, isolation of specific DNA fragments, PCR, cloning techniques, and gene expression. A Springer Lab Manual
Download or read book Hybridization with Nucleic Acid Probes Part I written by P. Tijssen and published by Elsevier Health Sciences. This book was released on 1993-10-21 with total page 336 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent breakthroughs in recombinant DNA technology and the availability of sophisticated equipment accessible to almost any laboratory, have contributed to the development and perfection of powerful hybridization tools. Recently, nucleic acid hybridization has not only become a cornerstone in molecular biology research but also a powerful supplement to other diagnostic tools. These diagnostic methods are set out in a logical and clear two-part volume in this now famous Laboratory Techniques series. The volume is divided into theory and preparation (Part I), and probe labelling and hybridization techniques (Part II). Both parts are worthy additions to this series, designed for easy access of information on the laboratory bench.
Download or read book Instant Notes in Molecular Biology written by Aulay Mackenzie and published by . This book was released on 1999 with total page 214 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Nucleic Acid Probes written by Robert H. Symons and published by CRC Press. This book was released on 1989-03-31 with total page 246 pages. Available in PDF, EPUB and Kindle. Book excerpt: With each chapter written by a distinguished expert in the biochemistry field, this comprehensive volume describes the preparation and use of a variety of radioactive and non-radioactive probes in situations ranging from research laboratories to routine diagnosis laboratories. The enzymatic and chemical techniques for labeling nucleic acid probes with radioisotopes and with non-radioactive ligands and haptens are discussed. Additionally, the associated methods for their detection, the use of these probes in the diagnosis of human and microbial pathogens, of plant viruses and viroids, and of human genetic disorders, as well as in the detection of nucleic acids in tissues and cells by in situ hybridization are presented.
Download or read book Nucleic Acid Sandwich Hybridization for Microbial Detection written by Airi Palva and published by . This book was released on 1985 with total page 74 pages. Available in PDF, EPUB and Kindle. Book excerpt:
