Download or read book Membrane Protein Structure Determination written by Jean-Jacques Lacapère and published by Methods in Molecular Biology. This book was released on 2010-08-06 with total page 482 pages. Available in PDF, EPUB and Kindle. Book excerpt: Membrane proteins, representing nearly 40% of all proteins, are key components of cells involved in many cellular processes, yet only a small number of their structures have been determined. Membrane Protein Structure Determination: Methods and Protocols presents many detailed techniques for membrane protein structure determination used today by bringing together contributions from top experts in the field. Divided into five convenient sections, the book covers various strategies to purify membrane proteins, approaches to get three dimensional crystals and solve the structure by x-ray diffraction, possibilities to gain structural information for a membrane protein using electron microscopy observations, recent advances in nuclear magnetic resonance (NMR), and molecular modelling strategies that can be used either to get membrane protein structures or to move from atomic structure to a dynamic understanding of a molecular functioning mechanism. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and easy to use, Membrane Protein Structure Determination: Methods and Protocols serves as an ideal reference for scientists seeking to further our knowledge of these vital and versatile proteins as well as our overall understanding of the complicated world of cell biology.
Download or read book Epitope Mapping Protocols written by Johan Rockberg and published by Humana. This book was released on 2018-05-01 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This third edition volume expands on the previous editions with more detailed research on the characterization of antibody antigen interactions between different users with different requirements. The chapters in this book are divided into four parts: Part One looks at the entire native antigen and covers traditional structural biology techniques such as nuclear magnetic resonance and x-ray crystallography. Part Two talks about protein fragments derived from antigens, and discusses binding regions within antigen sequence using bacterial surface display and ELISA, for example. Part Three describes the use of surface plasmon resonance spectroscopy and biolayer interferometry, and Part Four highlights methods used to identify new antigens and assess antibody cross-reactivity. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Epitope Mapping Protocols, Third Edition is a valuable resource for anyone interested in furthering their research in this expanding field.
Download or read book Analytical Characterization of Biotherapeutics written by Jennie R. Lill and published by John Wiley & Sons. This book was released on 2017-08-14 with total page 378 pages. Available in PDF, EPUB and Kindle. Book excerpt: The definitive guide to the myriad analytical techniques available to scientists involved in biotherapeutics research Analytical Characterization of Biotherapeutics covers all current and emerging analytical tools and techniques used for the characterization of therapeutic proteins and antigen reagents. From basic recombinant antigen and antibody characterization, to complex analyses for increasingly complex molecular designs, the book explores the history of the analysis techniques and offers valuable insights into the most important emerging analytical solutions. In addition, it frames critical questions warranting attention in the design and delivery of a therapeutic protein, exposes analytical challenges that may occur when characterizing these molecules, and presents a number of tested solutions. The first single-volume guide of its kind, Analytical Characterization of Biotherapeutics brings together contributions from scientists at the leading edge of biotherapeutics research and manufacturing. Key topics covered in-depth include the structural characterization of recombinant proteins and antibodies, antibody de novo sequencing, characterization of antibody drug conjugates, characterization of bi-specific or other hybrid molecules, characterization of manufacturing host-cell contaminant proteins, analytical tools for biologics molecular assessment, and more. Each chapter is written by a recognized expert or experts in their field who discuss current and cutting edge approaches to fully characterizing biotherapeutic proteins and antigen reagents Covers the full range of characterization strategies for large molecule based therapeutics Provides an up-to-date account of the latest approaches used for large molecule characterization Chapters cover the background needed to understand the challenges at hand, solutions to characterize these large molecules, and a summary of emerging options for analytical characterization Analytical Characterization of Biotherapeutics is an up-to-date resource for analytical scientists, biologists, and mass spectrometrists involved in the analysis of biomolecules, as well as scientists employed in the pharmaceuticals and biotechnology industries. Graduate students in biology and analytical science, and their instructors will find it to be fascinating and instructive supplementary reading.
Download or read book Nuclear Magnetic Resonance Studies in Plant Science written by Robert D. Warmbrodt and published by . This book was released on 1991 with total page 56 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Research Awards Index written by and published by . This book was released on 1978 with total page 1520 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Biomolecular NMR Spectroscopy written by Andrew J. Dingley and published by IOS Press. This book was released on 2011 with total page 488 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nuclear Magnetic Resonance (NMR) spectroscopy is the most powerful technique for characterization of biomolecular structures at atomic resolution in the solution state. This timely book, entitled "Biomolecular NMR Spectroscopy," focuses on the latest state-of-the-art NMR techniques for characterization of biological macromolecules in the solid and solution state. The editors, Dr. Andrew Dingley (University of Auckland, New Zealand) and Dr. Steven Pascal (Massey University, New Zealand) have organized the book into four sections, covering the following topics: sample preparation, structure and dynamics of proteins, structure and dynamics of nucleic acids and protein-nucleic acid complexes, and rapid and hybrid techniques--
Download or read book Allergy and Cross Reactivity written by Sue C. Killian M.S and published by Xlibris Corporation. This book was released on 2017-08-15 with total page 213 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book announces to the reader that allergens are molecules. Thats right. You are not allergic to the entire ragweed plant, the entire dust mite, or the whole cow, just one or more types of allergic molecule within each of those organisms. If you are allergic to a molecule in a dust mite, for example, you might be allergic to shrimp or even tropical fish food. If you are allergic to some pollens, you could also be allergic to nuts, fruits, and some vegetables because they may share those pesky allergenic molecules. Some of these shared allergenic molecules have been identified by scientists, and the reader will benefit by learning where they hide. The readers unexplained reactions could be caused by these cross-reactive molecules. This is a situation where a little bit of knowledge is dangerous because cross-reactivity as it is, often briefly mentioned in magazines, could create fear of foods. Alternatively, knowledge about cross-reactive molecules could uncover these specific allergens. Patients could easily avoid these cross-reactive pollen molecules if they knew they were in their foods, thereby eliminating much suffering from allergy. Why would a patient who tested positive to a pollen eat the same allergens in a food? If they knew about cross-reactivity, they would not. This book explains the allergic response and the many chemicals the body produces in response to an allergic reaction. Allergy is a serious medical condition, and a patient can unintentionally make their allergic reaction much worse by exposure to a similar cross-reactive allergen. Alternatively, in addition to the patients chosen medical treatment, knowing about cross-reactivity can provide the patient with additional self-help.
Download or read book Practical Applications of NMR to Solve Real World Problems written by Robert Brinson and published by Mdpi AG. This book was released on 2021-12-02 with total page 162 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nuclear magnetic resonance spectroscopy (NMR) has developed from primarily a method of academic study into a recognized technology that has advanced measurement capabilities within many different industrial sectors. These sectors include areas such as national security, energy, forensics, life sciences, pharmaceuticals, etc. Despite this diversity, these applications have many shared technical challenges and regulatory burdens, yet interdisciplinary cross-talk is often limited. To facilitate the sharing of knowledge, this Special Issue presents technical articles from four different areas, including the oil industry, nanostructured systems and materials, metabolomics, and biologics. These areas use NMR or magnetic resonance imaging (MRI) technologies that range from low-field relaxometry to magnetic fields as high as 700 MHz. Each article represents a practical application of NMR. A few articles are focused on basic research concepts, which will likely have the cross-cutting effect of advancing multiple disciplinary areas.
Download or read book Biomedical Index to PHS supported Research written by and published by . This book was released on 1989 with total page 776 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Compact NMR written by Bernhard Blümich and published by Walter de Gruyter GmbH & Co KG. This book was released on 2014-08-22 with total page 305 pages. Available in PDF, EPUB and Kindle. Book excerpt: The goal of this book is to provide an introduction to the practical use of mobile NMR at a level as basic as the operation of a smart phone. Each description follows the same didactic pattern: introduction, basic theory, pulse sequences and parameters, beginners-level measurements, advanced-level measurements, and data processing. Nuclear Magnetic Resonance (NMR) spectroscopy is the most popular method for chemists to analyze molecular structures while Magnetic Resonance Imaging (MRI) is a non-invasive diagnostic tool for medical doctors that provides high-contrast images of biological tissue depicting the brain function and the beating heart. In both applications large super-conducting magnets are employed which magnetize atomic nuclei of an object positioned inside the magnet. Their circulating motion is interrogated by radio-frequency waves. Depending on the operating mode, the frequency spectrum provides the chemist with molecular information, the medical doctor with anatomic images, while the materials scientist is interested in NMR relaxation parameters, which scale with material properties and determine the contrast in magnetic resonance images. Recent advances in magnet technology led to a variety of small permanent magnets, by which NMR spectra, images, and relaxation parameters can be measured with mobile and low-cost instruments.
Download or read book Asthma written by Thomas A.E. Platts-Mills and published by CRC Press. This book was released on 1999-04-27 with total page 260 pages. Available in PDF, EPUB and Kindle. Book excerpt: Asthma is a disease which, beginning in the 1980s, increased in the United States and other countries in both its prevalence and mortality. According to reports by the Center for Disease Control, the number of people with asthma increased by 42% in the last decade, with children, ethnic minorities and the urban poor being most at risk. Timely research on the causes and mechanisms behind the dramatic increase of asthma in the United States and other nations-to epidemic proportions-is presented in this volume. Investigators heading up eight research groups in asthma and/or allergy present their most recent findings, funded by the Center for Indoor Air Research. In addition to the work underway and discussions/suggestions on present research problems, ASTHMA: Causes and Mechanisms of an Epidemic Inflammatory Disease provides a chapter on the causes of asthma-and the reasons for its recent spread, so hotly discussed in the current literature. Researchers in the fields of asthma and allergy, health care providers, and others who follow research in either of these two developing areas will benefit from this book. The book is written at the level of one investigator to another.
Download or read book National Institute of Allergy and Infectious Diseases NIH written by Vassil St. Georgiev and published by Springer Science & Business Media. This book was released on 2010-07-29 with total page 434 pages. Available in PDF, EPUB and Kindle. Book excerpt: National Institute of Allergy and Infectious Diseases, NIH: Volume III: Intramural Research contains a broad overview of the research activity of the NIAID intramural scientists working in the Division of Intramural Research (DIR) and the Vaccine Research Center (VRC), both in the Bethesda campus, and the Rocky Mountains Research Laboratories. Each of these laboratories employs scientists internationally recognized as leaders in their fields of biomedical research. This volume focuses on individual research contributions by internationally known scientists doing research in the NIAID laboratories.
Download or read book Public Health Service Grants and Awards by the National Institutes of Health written by and published by . This book was released on 1974 with total page 404 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Biology of Hevea Rubber written by P.M. Priyadarshan and published by Springer. This book was released on 2017-06-09 with total page 252 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the second edition of this book, the origin, upkeep and latex harvest from the Hevea rubber tree are dealt with succinctly. New chapters have been included on Propagation Systems and Genetic Resources. The importance of Heterozygosis and Breeding is a new theme for the section on Breeding. A new chapter on Genomics and Molecular Breeding that focuses on the latest advancements on gene mapping, marker assisted selection and stimulation has been added. Lastly, ‘textboxes’ that highlight points and topics of significant interest are included in the new addition. Natural rubber has been an essential commodity not only for the tire industry but also for more than 50,000 products that holds elasticity as an attribute. The prime source of natural rubber worldwide is Hevea brasiliensis. Hevea rubber tree is an excellent example of how a soil-tree-atmosphere system can work in tandem. The retrieval of rubber through ‘injuring’ the tree on alternate days or once in three days or once in seven days, is indeed a unique arrangement followed universally that ensures income to the planter almost throughout the year. Every molecule of rubber is the end result of meticulous biochemical changes. Therefore the biology of Hevea rubber tree itself is a subject that aggregates science and technology for the realization of its industrial utility.
Download or read book Research Grants Index written by National Institutes of Health (U.S.). Division of Research Grants and published by . This book was released on 1975 with total page 1212 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Public Health Service Research Grants and Fellowships written by National Institutes of Health (U.S.). Division of Research Grants. Statistics and Analysis Branch and published by . This book was released on 1969 with total page 1692 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Cumulated Index Medicus written by and published by . This book was released on 1999 with total page 1860 pages. Available in PDF, EPUB and Kindle. Book excerpt:
