Download or read book Novel Polymer based Microfluidic Devices written by Chong Hu and published by . This book was released on 2018 with total page 142 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the first part, we proposed an "inside-out" fabrication strategy using a copper scaffold as the sacrificial template to create freestanding 3D microvascular structures containing branched tubular networks with alginate hydrogel. The microvascular structures produced with this method are strong enough to allow handling, biocompatible for cell culture, appropriately porous to allow diffusion of small molecules, while sufficiently dense to prevent blocking of channels when embedded in various types of gels. In addition, other materials and biomolecules could be pre-loaded in our hydrogel tubular networks by mixing them with alginate solution, and the thickness of tubule wall is tunable. Compared to other potential strategies of fabricating free-standing gel channel networks, our method is parallel processing using an industrially mass-producible template, making our method rapid, low-cost and scalable. We demonstrated cell culture in a nutrition gradient based on a microfluidic diffusion device made of agar, a hydrogel traditionally hard to microfabricate, by embedding the synthesized tubules into the agar gel. In this way, the freestanding hydrogel vascular network we produced is a universal functional unit that can be integrated with other gel-based devices to build up the supporting matrix for 3D cell culture outside the hydrogel vascular structure; allowing great convenience and flexibility 3D culture. The method is readily implementable to have broad applications in biomedicine and biology, such as vascular tissue regeneration, drug discovery, and delivery system in 3D culture.
Download or read book Biomedical Applications of Microfluidic Devices written by Michael R. Hamblin and published by Academic Press. This book was released on 2020-11-12 with total page 352 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biomedical Applications of Microfluidic Devices introduces the subject of microfluidics and covers the basic principles of design and synthesis of actual microchannels. The book then explores how the devices are coupled to signal read-outs and calibrated, including applications of microfluidics in areas such as tissue engineering, organ-on-a-chip devices, pathogen identification, and drug/gene delivery. This book covers high-impact fields (microarrays, organ-on-a-chip, pathogen detection, cancer research, drug delivery systems, gene delivery, and tissue engineering) and shows how microfluidics is playing a key role in these areas, which are big drivers in biomedical engineering research. This book addresses the fundamental concepts and fabrication methods of microfluidic systems for those who want to start working in the area or who want to learn about the latest advances being made. The subjects covered are also an asset to companies working in this field that need to understand the current state-of-the-art. The book is ideal for courses on microfluidics, biosensors, drug targeting, and BioMEMs, and as a reference for PhD students. The book covers the emerging and most promising areas of biomedical applications of microfluidic devices in a single place and offers a vision of the future. - Covers basic principles and design of microfluidics devices - Explores biomedical applications to areas such as tissue engineering, organ-on-a-chip, pathogen identification, and drug and gene delivery - Includes chemical applications in organic and inorganic chemistry - Serves as an ideal text for courses on microfluidics, biosensors, drug targeting, and BioMEMs, as well as a reference for PhD students
Download or read book Fabrication of Polymer Based Microfluidic Devices for Chemical and Biological Analysis written by Bo Shen and published by . This book was released on 2016 with total page 104 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Microfluidics for Advanced Functional Polymeric Materials written by Liang-Yin Chu and published by John Wiley & Sons. This book was released on 2017-03-13 with total page 336 pages. Available in PDF, EPUB and Kindle. Book excerpt: A comprehensive and systematic treatment of our current understanding of the microfluidic technique and its advantages in the controllable fabrication of advanced functional polymeric materials. Introducing and summarizing recent advances and achievements in the field, the authors cover the design and fabrication of microfluidic devices, the fundamentals and strategies for controllable microfluidic generation of multiphase liquid systems, and the use of these liquid systems with an elaborate combination of their structures and compositions for generating novel polymer materials, such as microcapsules, microfibers, valves, and membranes. Clear diagrams and illustrations throughout the text make the relevant theory and technologies more readily accessible. The result is a specialist reference for materials scientists, organic, polymer and physical chemists, and chemical engineers.
Download or read book Laser Assisted Fabrication of Polymer Based Microfluidic Devices written by Soni Chandrasekar and published by . This book was released on 2015 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Novel Polymeric Biochips for Enhanced Detection of Infectious Diseases written by Samira Hosseini and published by Springer. This book was released on 2015-11-21 with total page 63 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book focuses on the creation and development of polymeric platforms (different compositions) from a specific polymer system. This system can be used as an adaptive technique for producing sensitive analytical devices, or for simple integration into existing bioanalytical tools in order to enhance the detection signal.
Download or read book Fabrication and Application of Polymer Based Microfluidic Devices written by Myra T. Koesdjojo and published by . This book was released on 2009 with total page 206 pages. Available in PDF, EPUB and Kindle. Book excerpt: The concept of reducing laboratory operations in scale such that they fit on a microfluidic chip has been met with great enthusiasm. Lab-on-a-chip devices promise to be cost effective to operate due to reduced reagent consumption, have the potential to offer shorter analysis times due to their short path lengths, and may be useful in biological applications in that they are inherently compact and inexpensive to build, thus they may be disposable. In this work, a series of fabrication techniques for the production of polymer-based microfluidic devices are explored. In the first component of this research effort an aluminum mold was fabricated using CNC machining to create the desired microchannel design, followed by a two-stage embossing process, involving two polymer substrates with different glass transition temperatures (Tg), polyetherimide (PEI; Tg~216oC) and poly(methyl methacrylate) (PMMA; Tg~105oC). Successful feature transfer from aluminum mold to PMMA substrates was achieved reproducibly employing this method. With this approach, the expensive process of producing the aluminum master need be performed only once. Electrophoretic separations of fluorescent dyes, rhodamine B and fluorescein were performed on the PMMA microchips, with peak efficiencies of 55500 and 66300 theoretical plates/meter, respectively. The next stage of work explored a new bonding method by solvent welding using ice as sacrificial layer to prevent channel deformation. Water is one of the most compatible sacrificial media; it is readily available, non toxic, has a low evaporation rate, a high freezing point relative to the bonding solvent, and a low melting point which makes it easier to flush out after sealing, as compared to using other sacrificial media (paraffin wax or low-melting temperature alloys). The bonded PMMA microchips could withstand an internal pressure of > 2000 psi, more than 17 times stronger than the thermally bonded chips. In the final stage of work a new bonding technique was developed that readily produces complete microfluidic chips, without the need of a sacrificial layer to form complete multilayer microfluidic devices. Also developed was the use of an SU-8 master in the two-stage embossing process to create microchannels. This approach is faster, simpler and less costly than CNC machining. The fabrication technique was utilized to build a microfluidic liquid chromatography (LC) system that was shown to generate high separation efficiencies of 10,000- 45,000 plates/m. In addition, a passive micromixer containing high-density microfeatures was fabricated to perform a glycine assay using O-phthaldialdehyde. With glycine concentrations ranging from 0.0 to 2.6 [mu]M, a linear calibration plot (R2 = 0.9982) was obtained with a detection limit of 0.032 [mu]M.
Download or read book A Highly integrated Polymer based Microfluidic Device for Disposable Applications written by Emil John Geiger and published by . This book was released on 2008 with total page 494 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Polymer based Micromachining Technology for Microfluidic Devices written by Carlos H. Mastrangelo and published by . This book was released on 1998 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Polymer based Microfluidic Devices for High Throughput Single Molecule Detection written by Paul Ichide Okagbare and published by . This book was released on 2009 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Development of Sealing Techniques for Polymer based Microfluidic Devices Using High Frequency Electromagnetic Field written by Abdirahman Ali Yussuf and published by . This book was released on 2006 with total page 200 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Microfluidics written by Yu Song and published by John Wiley & Sons. This book was released on 2018-05-07 with total page 576 pages. Available in PDF, EPUB and Kindle. Book excerpt: The first book offering a global overview of fundamental microfluidics and the wide range of possible applications, for example, in chemistry, biology, and biomedical science. As such, it summarizes recent progress in microfluidics, including its origin and development, the theoretical fundamentals, and fabrication techniques for microfluidic devices. The book also comprehensively covers the fluid mechanics, physics and chemistry as well as applications in such different fields as detection and synthesis of inorganic and organic materials. A useful reference for non-specialists and a basic guideline for research scientists and technicians already active in this field or intending to work in microfluidics.
Download or read book Microfluidics for Biologists written by Chandra K. Dixit and published by Springer. This book was released on 2016-10-13 with total page 256 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book describes novel microtechnologies and integration strategies for developing a new class of assay systems to retrieve desired health information from patients in real-time. The selection and integration of sensor components and operational parameters for developing point-of-care (POC) are also described in detail. The basics that govern the microfluidic regimen and the techniques and methods currently employed for fabricating microfluidic systems and integrating biosensors are thoroughly covered. This book also describes the application of microfluidics in the field of cell and molecular biology, single cell biology, disease diagnostics, as well as the commercially available systems that have been either introduced or have the potential of being used in research and development. This is an ideal book for aiding biologists in understanding the fundamentals and applications of microfluidics. This book also: Describes the preparatory methods for developing 3-dimensional microfluidic structures and their use for Lab-on-a-Chip design Explains the significance of miniaturization and integration of sensing components to develop wearable sensors for point-of-care (POC) Demonstrates the application of microfluidics to life sciences and analytical chemistry, including disease diagnostics and separations Motivates new ideas related to novel platforms, valving technology, miniaturized transduction methods, and device integration to develop next generation sequencing Discusses future prospects and challenges of the field of microfluidics in the areas of life sciences in general and diagnostics in particular
Download or read book Additive Manufacturing of Polymer based Mcrofluidic Devices written by Vahid Karamzadeh and published by . This book was released on 2018 with total page 104 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microfluidics is a multidisciplinary area comprising several disciplines such as biology, chemistry and tissue engineering, which leads to manipulation and analysis of liquid through micro channels. Microfluidic devices provide numerous advantages for economical point-of-care diagnosis compared to conventional testing devices. In addition, more recently organ-on-a-chip which is a 3D microfluidic network for cell culturing has offered a microfluidic chip that stimulates the response of an organ system which can significantly reduce the costs of developing new pharmaceutical drugs. In spite of multiple advantages of microfluidic devices, barriers exist in current microfabrication methods. Fabrication of microfluidic molds requires cleanroom facilities and is a costly and tedious process which hindered the commercialization of microfluidic chips. Although, using 3D printed molds has been suggested to simplify fabrication of chips, their resolution is limited to 100 om. In addition to these factors, molding process is challenging to fully automate which means that it is in contrast to the vision of cost-effective and mass production of these devices. Therefore, a fully automated, rapid process is required to guide microfluidics towards the development of low-cost more commercially devices. In this thesis, different additive manufacturing methods for fabrication of polymeric microfluidic devices are presented. First, a commercial stereolithography (SLA) 3D printer was used to fabricate 3D printed molds. Limitations and accuracy of using 3D printed templates for microfluidic applications were investigated. Then, the thesis presents a promising additive manufacturing method toward printing a polymer-based device by using an acoustic assisted printing method. This method can significantly simplify and improve fabrication of microfluidic devices. Simulation of the acoustic wave and the heat induced by that were obtained in order to optimize the printing process prior to the experiment. The model provided a good understanding and estimation of acoustic field, temperature rise and focal region size. Time and cost required for fabricating a part by this method is considerably low and no additional post processing is required to turn the printed part to a functional part. The proposed method was finally applied to fabricate a fluidic channel. A fluidic channel can be printed in less than 10 minutes without adding additional components.
Download or read book Lysis of Bacteria with a Polymer based Microfluidic Device with Embedded Carbon Nanotubes written by David Harrison Altman and published by . This book was released on 2007 with total page 212 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Lab on a Chip written by R. Edwin Oosterbroek and published by Elsevier. This book was released on 2003-10-02 with total page 404 pages. Available in PDF, EPUB and Kindle. Book excerpt: I TECHNOLOGIES -- Hydrogels and polymers as components of a lab on a chip -- Microreplication technologies for polymer-based æTAS applications -- Silicon and glass micromachining for æTAS -- Surface chemistry in polymer microfluidic systems -- Plastic microfluidic devices: electrokinetic manipulations, life science applications, and production technologies -- II METHODS -- Transverse diffusion in microfluidic systems -- Nanoliter & picoliter liquid handling -- Micro sequential injection system for monitoring of metabolites extruded by cultured cells -- III CELL- & BEAD-BASED SYSTEMS -- Handling of beads in microfluidic devices for biotech applications -- Particles and molecules handling in micro channels -- Cell counting and cell sizing in microstructures -- IV APPLICATIONS -- Microfabricated capillary array electrophoresis: -- implementation and applications -- Microfluidic systems for analysis of the proteome with mass spectrometry -- Interfacing æTAS to matrix assisted laser desorpt ...
Download or read book An Introduction to Ultrathin Organic Films written by Abraham Ulman and published by Academic Press. This book was released on 2013-10-22 with total page 468 pages. Available in PDF, EPUB and Kindle. Book excerpt: The development of oriented organic monomolecular layers by the Langmuir-Blodgett (LB) and self-assembly (SA) techniques has led researchers toward their goal of assembling individual molecules into highly ordered architectures. Thus the continually growing contribution of LB and SA systems to the chemistry and physics of thin organic films is widely recognized. Equally well-known is the difficulty in keeping up to date with the burgeoning multidisciplinary research in this area. Dr. Ulman provides a massive survey of the available literature. The book begins with a section on analytical tools to broaden the understanding of the structure and properties of monolayers and films. Following sections discuss LB films, the preparation and properties of SA monolayers and films, the modeling of LB and SA monolayers, and the application of LB and SA films.
