Download or read book Novel Automated Platform for Proteoform Driven Top down Mass Spectrometry Proteomics written by John Rawson Corbett and published by . This book was released on 2017 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Top-Down proteomics studies protein complexity at the intact proteoform level in order to study chemical modifications, such as co-post translational modifications and non-enzymatic protein processing (e.g., redox active modifications, glycation). With this approach, information content associated with the diversity of chemical/biological processes, such as glycosylation, lipidation, and proteolysis that occur in vivo, is captured facilitating an enhanced representative observation of biological complexity. To obtain this information, a traditional Top-Down approach uses liquid chromatography separations in conjunction with mass spectrometry and database querying techniques in order to identify proteoforms. For example, this approach was used in a study highlighting differentially expressed levels of phosphor-proteoforms within cardiac myofilaments and their association with different degrees of congestive heart failure. Although these strategies have been well characterized, such an approach is not applicable towards large scale proteome analysis due to the high heterogeneity of expressed proteoforms. For this type of analysis, multiple dimensions of orthogonal chromatographic separations are used to antagonize proteoform complexity, with prior attempts identifying over 3,000 unique proteoforms from the HeLa S3 cell line. These Top-Down platforms have also been used towards completing proteome scale label-free quantitative studies; however, such approaches have often struggled due to limited quantitative dynamic range. Additionally, chromatographic separation strategies have been protein driven reducing proteoform observation to only the most abundant species, and in some cases a complete loss of proteoform information (i.e., related glycoproteoforms) due to limitations associated with charging/ionization efficiency, ion transfer, and mass spectrometer resolving power. To address these obstacles, a novel platform that utilizes the concept of isoelectric point separation has been implemented in order to complete chromatographic separations at the proteoform level. Utilizing high resolution in solution isoelectric focusing with superficially porous liquid chromatography and Fourier-transform mass spectrometry, a ~5x improvement of observed proteoforms from cardiac myofibril tissue (1D: 112 vs. 2D: 582 proteoforms) was determined with species ranging from 3 – 230 kDa in size. In addition, novel data processing strategies that are capable of distinguishing related proteoform information content separated into different mass spectra have been implemented with the objective to establish the three quantitative levels of Top-Down proteomics (proteoform, protein, and proteoform ratios). Standard proteins with different physiochemical properties and modification classes were studied to create calibration curves under non-spiked and spiked conditions (i.e., E. coli matrix effect) with a linear dynamic range of 102 – 103 and low femtomole limits of detection values established. Additionally, results indicate that proteoform ratio information content, outside of matrix effects, is independent of protein loading. To aid in automating the data processing strategies associated with mass spectral deconvolution and data binning procedures, triplicate E. coli proteome analyses have been completed with a sliding window approach illustrating reproducible spectral intensity values (~15.1% relative standard deviation) and chromatographic precision tolerances of ± 0.2 pI units and ± 12 seconds for weighted pI and hydrophobicity calculations respectively. Using this platform, Lipocalin-type Prostaglandin D-Synthase, a highly glycosylated cerebrospinal fluid (CSF) protein, was fully characterized with 200+ proteoforms identified, a 65x improvement compared to other non-pI based Top-Down platforms that are chromatographically protein driven. In the future, the completion of CSF proteome profiling investigations will contribute to the interpretation of changes in proteoform modifications and expression levels and the correlation to unique pathobiology associated with different neurodegenerative and neuroinflammatory diseases.
Download or read book Novel Algorithms and Software for the Acquisition and Analysis of Mass Spectrometry based Proteomics Data written by Austin Vincent Carr and published by . This book was released on 2023 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proteomics technologies enable the large-scale, high-throughput study of proteoforms inbiological systems. The foremost approach, bottom-up proteomics, can identify and quantify thousands of proteins. However, the analysis of proteoforms is hindered by the required enzymatic digestion. Top-down proteomics does not require enzymatic digestion, enabling proteoform-level analysis. The technical challenge of analyzing proteoforms has thwarted the proliferation of top-down approaches, particularly compared to the ubiquity of bottom-up proteomics. Recent advances in sample preparation have enabled the intact mass measurements of classes of proteoforms traditionally considered challenging for top-down proteomics. Consequently, key barriers to broader applicability remaining in top-down proteomics are data acquisition and data processing. An additional challenge to proteomics in general is data interpretation. Lists of thousands of quantified proteins and/or proteoforms per experiment are not interpretable without software solutions capable of converting the raw output of proteomics experiments into biologically meaningful and actionable insight. This dissertation describes software-based solutions designed to improve the acquisition, processing, and interpretation of proteomics data. Chapter 1 provides an overview of mass spectrometry and the three key challenges addressed by methods in this thesis. Chapter 2 address challenges in data acquisition by introducing automated database searching of a data independent acquisition method for top-down proteomics using a hybrid database search strategy. Chapter 3 describes a new deconvolution method for low-resolution mass spectrometry data processing, taking top-down proteomics beyond high-resolution instruments. Chapter 4 also describes improvements in top-down proteomics data processing, applying astronomical averaging with outlier rejection algorithms to mass spectrometry data. In Chapter 5, a description of the application MetaNetwork, a computer program designed to facilitate systems biology insights for proteomics data is given.
Download or read book Design of the First Analytical Platform for Automated Top Down Proteomics with Quadrupole Fourier Transform Ion Cyclotron Resonance Mass Spectrometry written by Steven M. Patrie and published by . This book was released on 2004 with total page 236 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Advancing Capillary Electrophoresis mass Spectrometry for Top down Proteomics written by Tian Xu and published by . This book was released on 2023 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Top-down proteomics (TDP) enables the proteome profiling of biological subjects at the proteoform level and understanding of differential functions associated with proteoform heterogeneity, such as sequence variation, post-translational modifications (PTMs), etc. Drastic advances on TDP technologies (e.g. sample preparation, separation/fractionation, fragmentation, bioinformatics, etc.) have been achieved in the past decades. Further improvements in separation remain desired for better analysis throughput and deeper proteome coverage. Capillary electrophoresis (CE), including capillary zone electrophoresis (CZE) and capillary isoelectric focusing (cIEF), provide superior separation performance for proteoforms. This dissertation focuses on the advancement of CE-MS-based tools on throughput, separation resolution, and capacity for TDP and utility of these tools for biological applications.In Chapter 2, we developed high-throughput and high-capacity cIEF-MS/MS platforms. The high-throughput platform enables efficient identification and quantification of proteoforms (less than one hour per run), whereas the high-capacity cIEF-MS/MS provides large number of proteoform identifications (IDs, more than 700 proteoforms in a single shot analysis) which is valuable for deep TDP. In Chapter 3, we further improved the stability and robustness of cIEF-MS platform using optimized linear polyacrylamide (LPA) capillary coating and catholyte with lower pH (pH~10). The work achieved high-resolution characterization and accurate isoelectric point (pI) determination of charge variants (~0.1 pI difference) of monoclonal antibodies (mAbs). In Chapter 4, we developed a nondenaturing cIEF-MS platform for ultrahigh resolution characterization of microheterogeneity of a variety of protein complexes. Typically, pI determinations of variants in protein complexes allow us to decipher how sequence or PTM variations modulate the pIs of the protein complexes. In Chapter 5, while CZE-MS/MS is a well-developed approach, for the first time, we coupled FAIMS to CZE-MS/MS to facilitate online gas-phase fractionation of proteoforms. The FAIMS greatly enhanced the sensitivity of the system and expanded the number of proteoform IDs, especially large proteoform IDs. The work renders CZE-FAIMS-MS/MS as a new powerful multidimensional platform for deep TDP.In Chapters 6 and 7, we applied cIEF-MS/MS and CZE-MS/MS for studying the sexual dimorphism of zebrafish brains and proteoform-level differences between metastatic and nonmetastatic colorectal cancer (CRC) cells, respectively. In Chapter 6, quantitative TDP of thousands of proteoforms from male and female zebrafish brains by cIEF-MS/MS based approach discovered various overexpressed proteoforms in male or female brains that are closely associated with hormone activity. In Chapter 7, We performed deep TDP study of non-metastatic and metastatic CRC cells (SW480 and SW620) using CZE-MS/MS based multidimensional platform and identified more than 20,000 proteoforms of over 2,000 proteins from the two cell lines, which presents around 5-folds higher number of proteoform IDs in comparison with previous TDP studies of human cancer cells. The work revealed significant discrepancies between the two isogenic cell lines regarding proteoform and single amino acid variant (SAAV) profiles. Quantitative data disclosed differentially expressed proteoforms between the two cell lines and their corresponding genes were connected to cancer pathways and networks.
Download or read book Development of Mass Spectrometry based Proteomic Approaches for the Study of Diseased Tissues written by Samantha Jo Knott and published by . This book was released on 2021 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The completion of the human genome project allowed scientists to gain a wealth of information and quickly led to the acknowledgement that the complexity of disease phenotypes cannot be explained by genomic information alone. This observation can be attributed to the diversity of the proteome, where sequence variants, alternative splicing of mRNA transcripts and post translational modifications (PTMs), mean a singular gene can result in an enormous number of protein variants or proteoforms. Proteoforms have been shown to influence numerous biological processes and correlate well to disease phenotypes. Thus, it is critical to map and characterize tissues at the proteoform level to increase our understanding of disease mechanisms and identify new therapeutic targets. Mass spectrometry (MS) based proteomics plays an integral role in bridging our gaps in understanding. However, technological challenges still exist, and many areas have yet to be fully explored.The development of new platforms for in-depth tumor tissue characterization is needed, and despite the immense potential of top-down MS, few platforms have been developed. While bottom-up (peptide level) MS is routinely employed to identify thousands of proteins in tissues, the platform is inferior for the characterization of proteoforms and associated PTMs. On the other hand, top-down MS looks at intact proteoforms and is a powerful method for the unambiguous identification of isoforms and PTMs. In this thesis work, I have met the need and developed a top-down proteomics platform to inventory the proteoform landscape of tumor tissues harvested from a well-established mouse model of metastatic breast cancer. Using a conventional platform, few large proteins >35 kDa were observed due to the challenge and reduced signal-to-noise high-MW proteoforms present for top-down MS. In this thesis, I utilized MS-compatible size-exclusion chromatography to provide greater separation power and expanded the coverage of large tumor proteoforms 5-fold, with the detection of species up to 104 kDa. Another under explored area in proteomic analysis of tumor tissues is the characterization and quantification of the extracellular matrix (ECM), primarily due to the inherent insolubility. I developed a quantitative bottom-up MS approach to effectively solubilize, digest, and identify ECM proteins in both tumor and cardiac tissues enabled by a MS-compatible photocleavable surfactant. Importantly, the method is more accessible and takes just eight hours, compared to the previous multi-day process.
Download or read book Proteoform Identification written by Liangliang Sun and published by Humana. This book was released on 2023-06-18 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume discusses the latest mass spectrometry (MS)-based technologies for proteoform identification, characterization, and quantification. Some of the topics covered in this book include sample preparation, proteoform separation, proteoform gas-phase fragmentation, and bioinformatics tools for MS data analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Proteoform Identification: Methods and Protocols is a valuable resource for researchers in both academia and the biopharmaceutical industry who are interested in proteoform analysis using MS.
Download or read book New Front end Separation Approaches for Top down Proteomics written by Eli Larson and published by . This book was released on 2023 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Top-down mass spectrometry (MS) and top-down proteomics have become indispensable tools to characterize and identify unique proteoforms. Proteoforms are defined as all protein products of a single gene, including splicing variants, mutants, and post-translationally modified forms. Although the development of new MS capabilities has exploded in recent years, the comparative underdevelopment of intact protein separations and data processing solutions has prevented full realization of the benefits of top-down. To address these challenges, I have developed new front-end separation approaches for top-down proteomics, beginning with targeted separations for multi-attribute analysis of antibody-drug conjugates (ADCs) and later developing an online two-dimensional liquid chromatography (2DLC) method to expand global proteome coverage by top-down proteomics. Chapter 1 focuses on recent advances in front-end separations and data processing solutions for top-down proteomics and introduces top-down applications to antibody-based therapeutic analysis. Chapter 2 and chapter 3 detail new targeted separation approaches for monoclonal antibodies and ADCs. Chapter 2 reports reversed phase liquid chromatography (RPLC) coupled to high-resolution Fourier transform ion cyclotron resonance MS for top-down analysis of a reduced cysteine-linked ADC. Chapter 3 details the development of a native complex-down workflow using trapped ion mobility spectrometry-MS with a cysteine-linked ADC and parent mAb under non-denaturing conditions (Chapter 3). Chapter 4 reports a new software package designed to address the challenges associated with native top-down proteomics, MASH Native. Chapter 5 focuses on the development of a new online 2DLC method coupling serial size exclusion and RPLC to expand global top-down proteome coverage, with application to human heart extract. Appendix I reports a shotgun proteomic approach to characterize the impact of splicing factor RNA binding motif 20 knockout on the rat heart proteome and identifies targets for follow-up analysis by top-down proteomics. The developed techniques detailed here will address key challenges to front-end separation in the field of top-down proteomics, expanding analytical capabilities for future targeted and discovery studies.
Download or read book Chapter Preparing Proteoforms of Therapeutic Proteins for Top Down Mass Spectrometry written by Laura Heikaus and published by . This book was released on 2019 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: A characteristic of many proteoforms, derived from a single gene, is their similarity regarding the composition of atoms, making their analysis very challenging. Many overexpressed recombinant proteins are strongly associated with this problem, especially recombinant therapeutic glycoproteins from large-scale productions. In contrast to small molecule drugs, which consist of a single defined molecule, therapeutic protein preparations are heterogenous mixtures of dozens or even hundreds of very similar species. With mass spectrometry, currently high-quality spectra of intact proteoforms can be obtained only, if the complexity of the mixture of individual proteoform-ions, entering the gas phase at the same time is low. Thus, prior to mass spectrometric analysis, an effective separation is required for getting fractions with a low number of individual proteoforms. This is especially true not only for recombinant therapeutic proteins, because of their huge heterogeneity, but also relevant for top-down proteomics. Purification of proteoforms is the bottleneck in analyzing intact proteoforms with mass spectrometry. This review is focusing on the current state of the art, especially of liquid chromatography for preparing proteoforms for mass spectrometric top-down analysis. The topic of therapeutic proteins has been chosen, because this group of proteins is most challenging regarding their proteoform analysis.
Download or read book Encyclopedia of Bioinformatics and Computational Biology written by and published by Elsevier. This book was released on 2018-08-21 with total page 3421 pages. Available in PDF, EPUB and Kindle. Book excerpt: Encyclopedia of Bioinformatics and Computational Biology: ABC of Bioinformatics, Three Volume Set combines elements of computer science, information technology, mathematics, statistics and biotechnology, providing the methodology and in silico solutions to mine biological data and processes. The book covers Theory, Topics and Applications, with a special focus on Integrative –omics and Systems Biology. The theoretical, methodological underpinnings of BCB, including phylogeny are covered, as are more current areas of focus, such as translational bioinformatics, cheminformatics, and environmental informatics. Finally, Applications provide guidance for commonly asked questions. This major reference work spans basic and cutting-edge methodologies authored by leaders in the field, providing an invaluable resource for students, scientists, professionals in research institutes, and a broad swath of researchers in biotechnology and the biomedical and pharmaceutical industries. Brings together information from computer science, information technology, mathematics, statistics and biotechnology Written and reviewed by leading experts in the field, providing a unique and authoritative resource Focuses on the main theoretical and methodological concepts before expanding on specific topics and applications Includes interactive images, multimedia tools and crosslinking to further resources and databases
Download or read book Plant Proteomics written by Jozef Samaj and published by Springer Science & Business Media. This book was released on 2007-09-09 with total page 397 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant Proteomics highlights rapid progress in this field, with emphasis on recent work in model plant species, sub-cellular organelles, and specific aspects of the plant life cycle such as signaling, reproduction and stress physiology. Several chapters present a detailed look at diverse integrated approaches, including advanced proteomic techniques combined with functional genomics, bioinformatics, metabolomics and molecular cell biology, making this book a valuable resource for a broad spectrum of readers.
Download or read book Youmans and Winn Neurological Surgery E Book written by H. Richard Winn and published by Elsevier Health Sciences. This book was released on 2022-01-21 with total page 7439 pages. Available in PDF, EPUB and Kindle. Book excerpt: Widely regarded as the definitive reference in the field, Youmans and Winn Neurological Surgery offers unparalleled, multimedia coverage of the entirety of this complex specialty. Fully updated to reflect recent advances in the basic and clinical neurosciences, the 8th Edition covers everything you need to know about functional and restorative neurosurgery, deep brain stimulation, stem cell biology, radiological and nuclear imaging, and neuro-oncology, as well as minimally invasive surgeries in spine and peripheral nerve surgery, and endoscopic and other approaches for cranial procedures and cerebrovascular diseases. In four comprehensive volumes, Dr. H. Richard Winn and his expert team of editors and authors provide updated content, a significantly expanded video library, and hundreds of new video lectures that help you master new procedures, new technologies, and essential anatomic knowledge in neurosurgery. - Discusses current topics such as diffusion tensor imaging, brain and spine robotic surgery, augmented reality as an aid in neurosurgery, AI and big data in neurosurgery, and neuroimaging in stereotactic functional neurosurgery. - 55 new chapters provide cutting-edge information on Surgical Anatomy of the Spine, Precision Medicine in Neurosurgery, The Geriatric Patient, Neuroanesthesia During Pregnancy, Laser Interstitial Thermal Therapy for Epilepsy, Fetal Surgery for Myelomeningocele, Rehabilitation of Acute Spinal Cord Injury, Surgical Considerations for Patients with Polytrauma, Endovascular Approaches to Intracranial Aneurysms, and much more. - Hundreds of all-new video lectures clarify key concepts in techniques, cases, and surgical management and evaluation. Notable lecture videos include multiple videos on Thalamotomy for Focal Hand Dystonia and a video to accompany a new chapter on the Basic Science of Brain Metastases. - An extensive video library contains stunning anatomy videos and videos demonstrating intraoperative procedures with more than 800 videos in all. - Each clinical section contains chapters on technology specific to a clinical area. - Each section contains a chapter providing an overview from experienced Section Editors, including a report on ongoing controversies within that subspecialty. - Enhanced eBook version included with purchase. Your enhanced eBook allows you to access all of the text, figures, and references from the book on a variety of devices.
Download or read book Proteomics Sample Preparation written by Jörg von Hagen and published by John Wiley & Sons. This book was released on 2011-08-24 with total page 498 pages. Available in PDF, EPUB and Kindle. Book excerpt: This long-awaited first guide to sample preparation for proteomics studies overcomes a major bottleneck in this fast growing technique within the molecular life sciences. By addressing the topic from three different angles -- sample, method and aim of the study -- this practical reference has something for every proteomics researcher. Following an introduction to the field, the book looks at sample preparation for specific techniques and applications and finishes with a section on the preparation of sample types. For each method described, a summary of the pros and cons is given, as well as step-by-step protocols adaptable to any specific proteome analysis task.
Download or read book Epigenetics in Human Disease written by Trygve O Tollefsbol and published by Elsevier. This book was released on 2023-10-09 with total page 1362 pages. Available in PDF, EPUB and Kindle. Book excerpt: Epigenetics in Human Disease, Third Edition examines the diseases and conditions on which we have advanced knowledge of epigenetic mechanisms, such as cancer, autoimmune disorders, aging, metabolic disorders, neurobiological disorders and cardiovascular disease. From molecular mechanisms and epigenetic technology to clinical translation of recent research, the nature and applications of the science is presented for those with interests ranging from the fundamental basis of epigenetics to therapeutic interventions for epigenetic-based disorders, with an emphasis throughout on understanding and application of key concepts in new research and clinical practice. Fully revised and up-to-date, this Third Edition discusses topics of current interest in epigenetic disease research, including stem cell epigenetic therapy, bioinformatic analysis of NGS data, epigenetic mechanisms of imprinting disorders, microRNA in cancer, epigenetic approaches to control obesity, epigenetics and airway disease, and epigenetics in cardiovascular disease. Further sections explore online epigenetic tools and datasets; early-life programming of epigenetics in age-related diseases; the epigenetics of addiction and suicide, and epigenetic approaches to regulating and preventing diabetes, cardiac disease, allergic disorders, Alzheimer's disease, respiratory diseases, and many other human maladies. In addition, each chapter now includes chapter summaries, definitions, and vibrant imagery and figures to reinforce understanding, as well as step-by-step methods and disease research case studies. - Includes contributions from leading international investigators involved in translational epigenetic research and therapeutic applications - Integrates methods and applications with fundamental chapters on epigenetics in human disease, along with an evaluation of recent clinical breakthroughs - Presents side-by-side coverage of the basis of epigenetic diseases and treatment pathways - Each chapter updated to include summaries, definitions, and vibrant imagery and figures to reinforce understanding - Features step-by-step methods and disease research case studies to put book concepts into practice
Download or read book Fundamentals and Applications of Fourier Transform Mass Spectrometry written by Philippe Schmitt-Kopplin and published by Elsevier. This book was released on 2019-08-11 with total page 780 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fundamentals and Applications of Fourier Transform Mass Spectrometry is the first book to delve into the underlying principles on the topic and their linkage to industrial applications. Drs. Schmitt-Kopplin and Kanawati have brought together a team of leading experts in their respective fields to present this technique from many different perspectives, describing, at length, the pros and cons of FT-ICR and Orbitrap. Numerous examples help researchers decide which instruments to use for their particular scientific problem and which data analysis methods should be applied to get the most out of their data. - Covers FT-ICR-MS and Orbitrap's fundamentals, enhancing researcher knowledge - Includes details on ion sources, data processing, chemical analysis and imaging - Provides examples across the wide spectrum of applications, including omics, environmental, chemical, pharmaceutical and food analysis
Download or read book Subcellular Proteomics written by Eric Bertrand and published by Springer Science & Business Media. This book was released on 2007-08-29 with total page 397 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume summarizes the new developments that made subcellular proteomics a rapidly expanding area. It examines the different levels of subcellular organization and their specific methodologies. In addition, the book includes coverage of systems biology that deals with the integration of the data derived from these different levels to produce a synthetic description of the cell as a system.
Download or read book MALDI TOF and Tandem MS for Clinical Microbiology written by Haroun N. Shah and published by John Wiley & Sons. This book was released on 2017-03-31 with total page 850 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book highlights the triumph of MALDI-TOF mass spectrometry over the past decade and provides insight into new and expanding technologies through a comprehensive range of short chapters that enable the reader to gauge their current status and how they may progress over the next decade. This book serves as a platform to consolidate current strengths of the technology and highlight new frontiers in tandem MS/MS that are likely to eventually supersede MALDI-TOF MS. Chapters discuss: Challenges of Identifying Mycobacterium to the Species level Identification of Bacteroides and Other Clinically Relevant Anaerobes Identification of Species in Mixed Microbial Populations Detection of Resistance Mechanisms Proteomics as a biomarker discovery and validation platform Determination of Antimicrobial Resistance using Tandem Mass Spectrometry
Download or read book Proteomics Data Analysis written by Daniela Cecconi and published by . This book was released on 2021 with total page 326 pages. Available in PDF, EPUB and Kindle. Book excerpt: This thorough book collects methods and strategies to analyze proteomics data. It is intended to describe how data obtained by gel-based or gel-free proteomics approaches can be inspected, organized, and interpreted to extrapolate biological information. Organized into four sections, the volume explores strategies to analyze proteomics data obtained by gel-based approaches, different data analysis approaches for gel-free proteomics experiments, bioinformatic tools for the interpretation of proteomics data to obtain biological significant information, as well as methods to integrate proteomics data with other omics datasets including genomics, transcriptomics, metabolomics, and other types of data. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detailed implementation advice that will ensure high quality results in the lab. Authoritative and practical, Proteomics Data Analysis serves as an ideal guide to introduce researchers, both experienced and novice, to new tools and approaches for data analysis to encourage the further study of proteomics.
