Download or read book Nonsense Mediated mRNA Decay written by Lynne E. Maquat and published by CRC Press. This book was released on 2006-02-28 with total page 277 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nonsense-Mediated mRNA Decay is the first book devoted to nonsense-mediated mRNA decay (NMD). The rationale for such a book is the enormous information that studies of NMD have provided on the intricacies of post-transcriptional gene expression. The first five sections of the book are divided according to organism and begin with chapters on S. cere

Download or read book Nonsense Mediated mRNA Decay written by Lynne E. Maquat and published by CRC Press. This book was released on 2006-02-28 with total page 277 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nonsense-Mediated mRNA Decay is the first book devoted to nonsense-mediated mRNA decay (NMD). The rationale for such a book is the enormous information that studies of NMD have provided on the intricacies of post-transcriptional gene expression. The first five sections of the book are divided according to organism and begin with chapters on S. cerevisiae and mammals, from which most NMD data derive. Chapters within these sections discuss the two basic ways cells differentiate between a termination codon that elicits NMD and one that does not. Nonsense-Mediated mRNA Decay makes clear that studies of NMD provide a unique opportunity to examine the coupling of mRNA translation and decay, and relationships between mRNP structure and function. The importance of NMD is evident from descriptions of its influence on the expression of genes that are essential for many cellular processes, including cell differentiation, cell maintenance and cell death. This book is, essentially, meant to be a one-stop source of information that fuels the fires of future experimentation.

Download or read book RNA Turnover in Eukaryotes Analysis of Specialized and Quality Control RNA Decay Pathways written by Lynne E. Maquat and published by Academic Press. This book was released on 2008-12-29 with total page 464 pages. Available in PDF, EPUB and Kindle. Book excerpt: Specific complexes of protein and RNA carry out many essential biological functions, including RNA processing, RNA turnover, and RNA folding, as well as the translation of genetic information from mRNA into protein sequences. Messenger RNA (mRNA) decay is now emerging as an important control point and a major contributor to gene expression. Continuing identification of the protein factors and cofactors and mRNA instability elements responsible for mRNA decay allow researchers to build a comprehensive picture of the highly orchestrated processes involved in mRNA decay and its regulation. * Covers the nonsense-mediated mRNA decay (NMD) or mRNA surveillance pathway * Expert researchers introduce the most advanced technologies and techniques * Offers step-by-step lab instructions, including necessary equipment and reagents

Download or read book Physiological Role and Quantitative Aspects of Human Nonsense mediated MRNA Decay NMD written by Marcelo Viegas and published by . This book was released on 2007 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Nonsense Mutation Correction in Human Diseases written by Fabrice Lejeune and published by Academic Press. This book was released on 2016-02-26 with total page 192 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nonsense Mutation Correction in Human Diseases: An Approach for Targeted Medicine provides an introduction on genetic diseases, discusses the prevalence of nonsense mutations, the consequences of a nonsense mutation for the expression of the mutant gene, and the presentation of the nonsense-mediated mRNA decay (NMD). It presents the mechanism of action and rationale associated with each strategy to correct nonsense mutations with the results of clinical trials to further support this basis. In addition, the book shows how it may be possible to combine several of these strategies to ultimately improve the efficiency of correction, also suggesting the future goals and objectives to improve treatment modalities in this evolving sphere of personalized medicine. Features basic biological and clinical constructs that inform the application of genomic data to clinical decision-making Includes theories and methods that can be used to link bio-molecular and clinical phenotypes so as to enable integrative hypothesis discovery, testing, and downstream evidence-based practice Provides design patterns and use cases that contextualize the clinical decision-making and evidence-based practice relative to real world requirements and stakeholders

Download or read book Insights Into Nonsense mediated MRNA Decay and Its Role in Modulating MRNA and Protein Levels in Mammalian Cells written by Dylan Brent Udy and published by . This book was released on 2021 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nonsense-mediated mRNA decay (NMD) is an essential, highly conserved quality control pathway that detects and degrades mRNAs containing premature termination codons (PTCs). Although the essentiality of NMD is frequently ascribed to its prevention of truncated protein accumulation, the extent to which NMD actually suppresses proteins encoded by NMD-sensitive transcripts is less well-understood than NMD-mediated suppression of mRNA. Here, we describe a reporter system that permits accurate quantification of both mRNA and protein levels via stable integration of paired reporters encoding NMD-sensitive and NMD-insensitive transcripts into the AAVS1 safe harbor loci in human cells. We use this system to demonstrate that NMD suppresses proteins encoded by NMD-sensitive transcripts by up to ~8-fold more than the mRNA itself. Our data indicate that NMD limits the accumulation of proteins encoded by NMD substrates by mechanisms beyond mRNA degradation, such that even when NMD-sensitive mRNAs escape destruction, their encoded proteins are still effectively suppressed.

Download or read book Analysis of Nonsense mediated MRNA Decay in Caenorhabditis Elegans written by Brian M. Cali and published by . This book was released on 1995 with total page 368 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Natural Targets of the Nonsense mediated MRNA Decay Pathway in Yeast written by Qiaoning Guan and published by . This book was released on 2006 with total page 232 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Nonsense mediated MRNA Decay NMD in Zebrafish written by Nadine Wittkopp and published by . This book was released on 2010 with total page 211 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Nonsense Mediated MRNA Decay of the Human HEXA MRNA written by Kavitha Swaminathan Rajavel and published by . This book was released on 2001 with total page 190 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Molecular Biology of The Cell written by Bruce Alberts and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Protein Sensors and Reactive Oxygen Species written by Helmut Sies and published by Academic Press. This book was released on 2002 with total page 550 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume of Methods in Enzymology is concerned with the rapidly developing field of selenoprotein synthesis and its related molecular genetics. Progressive information on the topics of proteins as redox sensors, selenoproteins, and the thioredoxin system is studied using methods such as bioinformatics, DNA chip technology, cell biology, molecular genetics, and enzymology. The information on novel selenoproteins identified from genomic sequence data, as well as current knowledge on glutathione peroxidases, selenoprotein P, iodothyronine deiodinases, and thioredoxin reductases, is presented in a method-based approach.

Download or read book The Nonsense mediated MRNA Decay Pathway written by Iván J. Cajigas and published by . This book was released on 2008 with total page 280 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Identification and Characterization of Factors Involved in the Nonsense mediated MRNA Decay Pathway written by Ying Cui and published by . This book was released on 1997 with total page 422 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Structural Studies on Proteins Involved in Nonsense mediated MRNA Decay written by Jan Kadlec and published by . This book was released on 2005 with total page 139 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nonsense-mediated mRNA decay (NMD) is a surveillance mechanism by which eukaryotic cells detect and degrade transcripts containing premature termination codons. Three 'up-frameshift' proteins, UPF1, UPF2 and UPF3, are essential for this process in organisms ranging trom yeast to human. We solved a crystal structure at a resolution of 1.95 A of the complex between the interacting domains of human UPF2 and UPF3b, which are, respectively, a MIF4G (middle portion of eIF4G) domain and an RRM (RNA recognition motif) domain. The protein-protein interface is mediated by highly conserved charged residues in UPF2 and UPF3b and involves the beta-sheet surface of the UPF3b RRM domain, which is generally used by these domains to bind nucleic acids. We show that the UPF3b RNP does not bind RNA, whereas the UPF2 construct and the complex do. We also obtained diffracting crystals of the complex consisting of the interacting domains UPF2 and UPF1. Finally, we determined the structure of the UPF2-interacting domain of UPF1 at a resolution of 3.0 A. Our results advance understanding of the molecular mechanisms underlying the NMD quality control process.

Download or read book Characterization of Nonsense Mediated MRNA Decay in Mammalian Cells written by Manuel Selg and published by . This book was released on 2002 with total page 450 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Nonsense mediated MRNA Decay in Collagen X written by and published by . This book was released on 2010 with total page 190 pages. Available in PDF, EPUB and Kindle. Book excerpt: