Download or read book NMR as a Structural Tool for Macromolecules written by M.D. Kemple and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 373 pages. Available in PDF, EPUB and Kindle. Book excerpt: The contemplation of truth and beauty is the proper object for which we were created, which calls forth the most intense desires of the soul, and of which it never tires -Hazlitt In his Nobel lecture Purcell commented that when he saw snow in New England after the discovery of NMR, it appeared like "heaps of protons quietly precessing in earth's magnetic field. " If he were to make the comment in the context of how NMR is being used today, he could have conjured up an image of hydrogen, carbon, and nitrogen nuclei in proteins of an earthbound 8rganism subtly orchestrating a quiet symphony of frequencies, from 150 Hz to 2 kHz, carrying clues to the three-dimensional structure of the macromolecules. The manner in which the basic discoveries of Bloch and Purcell have led to the emergence of NMR, several decades later, as a major technique of biological and medical physics (and chemistry) is a striking example of the power of basic research. It is also a fascinating saga whereby whenever it was felt that the field had reached a plateau, new directions, new technologies, and sometimes serendipity produced new developments that revolutionized the technique and enhanced its capability. As Richard Ernst points out "NMR is intellectually attractive, . . . the practical importance of NMR is enormous, and can justify much of the playful activities of an addicted spectroscopist" (Nobel lecture).

Download or read book NMR with Biological Macromolecules in Solution written by Kurt Wüthrich and published by World Scientific Publishing Company. This book was released on 2021 with total page 270 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book provides insights into the research of the Kurt Wüthrich laboratories from 1996-2020. During this time period, the technique of nuclear magnetic resonance (NMR) spectroscopy in solution went through several breakthroughs, while maturing into a standard method of structural biology. With the introduction of TROSY (transverse relaxation-optimized spectroscopy), the range of accessible molecular sizes was extended about thirty-fold, and efficient protein structure determination resulted from the demands of the structural genomics initiative. Applications in fundamental biology and biomedicine include studies of prion proteins and prion diseases (TSEs), the SARS-Corona virus proteome, trans-membrane signalling by G protein-coupled receptors (GPCRs), and signal transfer by pheromones.Key publications from the Kurt Wüthrich laboratories are placed in perspective, providing insights into new aspects of NMR spectroscopy in structural biology. In addition to methods development, this includes applications in diverse areas of biological research, such as prion proteins and their role in transmissible spongiform encephalopathies (TSEs), trans-membrane signal transfer by G protein-coupled receptors (GPCRs), structural characterization of the SARS-Corona virus proteome, metabolic-flux profiling in bacterial cultures, and signal transfers by pheromones.

Download or read book Nmr In Structural Biology A Collection Of Papers By Kurt Wuthrich written by Kurt Wuthrich and published by World Scientific. This book was released on 1995-07-31 with total page 760 pages. Available in PDF, EPUB and Kindle. Book excerpt: The volume presents a survey of the research by Kurt Wüthrich and his associates during the period 1965 to 1994. A selection of reprints of original papers on the use of NMR spectroscopy in structural biology is supplemented with an introduction, which outlines the foundations and the historical development of the use of NMR spectroscopy for the determination of three-dimensional structures of biological macromolecules in solution. The original papers are presented in groups highlighting protein structure determination by NMR, studies of dynamic properties and hydration of biological macromolecules, and practical applications of the NMR methodology in fields such as enzymology, transcriptional regulation, immunosuppression and protein folding.

Download or read book High Resolution NMR of Macromolecules written by Frank Bovey and published by Elsevier. This book was released on 2012-12-02 with total page 477 pages. Available in PDF, EPUB and Kindle. Book excerpt: High Resolution NMR of Macromolecules presents the development in the NMR study of polymers. This book discusses the exciting area of application of NMR to polymer science as the result of the more general accessibility of instruments of high magnetic field. Organized into 15 chapters, this book begins with an overview of the spectral analysis and the dependence of chemical shifts and J couplings on structure. This text then discusses the isomerism in polymer chains without special reference to NMR. Other chapters consider the interpretation of synthetic polymer spectra in terms of structure, stereochemical configuration, conformation, and chain growth mechanism. This book discusses as well the application of high resolution NMR to the study of nucleic acids, which has not been so well developed as that of polypeptides and proteins. The final chapter deals with biopolymers and their model compounds. This book is a valuable resource for chemists and research workers.

Download or read book Fundamentals of Protein NMR Spectroscopy written by Gordon S. Rule and published by Springer Science & Business Media. This book was released on 2005-10-28 with total page 543 pages. Available in PDF, EPUB and Kindle. Book excerpt: NMR spectroscopy has proven to be a powerful technique to study the structure and dynamics of biological macromolecules. Fundamentals of Protein NMR Spectroscopy is a comprehensive textbook that guides the reader from a basic understanding of the phenomenological properties of magnetic resonance to the application and interpretation of modern multi-dimensional NMR experiments on 15N/13C-labeled proteins. Beginning with elementary quantum mechanics, a set of practical rules is presented and used to describe many commonly employed multi-dimensional, multi-nuclear NMR pulse sequences. A modular analysis of NMR pulse sequence building blocks also provides a basis for understanding and developing novel pulse programs. This text not only covers topics from chemical shift assignment to protein structure refinement, as well as the analysis of protein dynamics and chemical kinetics, but also provides a practical guide to many aspects of modern spectrometer hardware, sample preparation, experimental set-up, and data processing. End of chapter exercises are included to emphasize important concepts. Fundamentals of Protein NMR Spectroscopy not only offer students a systematic, in-depth, understanding of modern NMR spectroscopy and its application to biomolecular systems, but will also be a useful reference for the experienced investigator.

Download or read book NMR of Biological Macromolecules written by Chariklia Ioannidou Stassinopoulou and published by Springer. This book was released on 1994-01-01 with total page 357 pages. Available in PDF, EPUB and Kindle. Book excerpt: Provided here are the latest techniques of NMR as applied to the study of proteins, carbohydrates and nucleic acids. The first chapters are devoted to an introduction to NMR and parameters related to molecular structure and molecular interactions. NMR experiments from basic 1D to 2D, 3D and 4D, used in combination with isotopically labelled molecules, are described and a general strategy is presented for biomacromolecular structure determination. Subsequent chapters deal with more advanced principles and techniques and their applications to structural and dynamic processes involving biomacromolecules in solution. Advanced results on peptide, protein, oligosaccharide and nucleic acid structure and recognition are presented.

Download or read book Computational Aspects of the Study of Biological Macromolecules by Nuclear Magnetic Resonance Spectroscopy written by Jeffrey C. Hoch and published by Springer. This book was released on 1991 with total page 488 pages. Available in PDF, EPUB and Kindle. Book excerpt: Without computers - no modern NMR; Parametric estimation in 1-D, 2-D, and 3-D NMR; Computational aspects of multinuclear NMR spectroscopy of proteins at NMRFAM; Principles of multidimensional NMR techniques for measurement of J coupling constants; Comparison of the NMR and X-ray structures of hirudin; The application of the linear prediction principle to NMR spectroscopy; NMR data processing and structure calculations using parallel computers; Software approaches for determination of 3-dimensional molecular structures from multi-dimensional NMR; Applicability and limitations of three-dimensional NMR spectroscopy for the study of proteins in solution; The role of selective two-dimensional NMR correlation methods in supplementing computer-supported multiplet analysis by MARCO POLO; Application of maximum entropy methods to NMR spectra of proteins; Pattern recognition in two-dimensional NMR spectra of proteins; The application and development of software tools for the processinf and analysis of heteronuclear multi-dimensional NMR data; Distance geometry in torsion angle space: new developments and applications; Structure determination by NMR: the modeling of NMR parameters as ensemble averages; Time averaged distance restraints in NMR based structural refinement; Analysis of backbone dynamics of interleukin-1 beta; A new version of DADAS (Distance Analysis in Dihedral Angle Space) and its performance; An amateur looks at error analysis in the determination of protein structure by NMR; Structural interpretation of NMR data in the presence of motion; New interactive and automatic algorithms for the assignment of NMR spectra; Outline of a computer program for the analysis of protein NMR spectra; Assignment of the NMR spectra of homologous proteins; Incorporation of internal motion in NMR refinements based on NOESY data; Refinement of three-dimensional protein and DNA structures in solution from NMR data; How to deal with spin-diffusion and internal mobility in biomolecules: a relaxation matrix approach; Interactive computer graphics in the assignment of protein 2D and 3D NMR spectra; Determination of large protein structures from NMR data: definition of the solution structure of the TRP repressor; Interpretation of NMR data in terms of protein structure: summary of a round table discussion; Fast calculation of the relaxation matrix; NMR structures of proteins using stereospecific assignments and relaxation matrix refinement in a hybrid method of distance geometry and simulated annealing; A critique of the interpretation of nuclear Overhauser effects of duplex DNA; Improvement in resolution with nonlinear methods applied to NMR signals from macromolecules; STELLA and CLAIRE: a seraglio of programs for human-aided assignment of 2D 1H NMR spectra of proteins; MolSkop: towards NMR molecular scope; Ribonuclease H: full assignment of backbone proton resonances with heteronuclear 3D NMR and solution structure; Sampling properties of simulated annealing and distance geometry.

Download or read book Structural Biology written by Quincy Teng and published by Springer Science & Business Media. This book was released on 2012-09-06 with total page 440 pages. Available in PDF, EPUB and Kindle. Book excerpt: This 2nd edition begins with an overview of NMR development and applications in biological systems. It describes recent developments in instrument hardware and methodology. Chapters highlight the scope and limitation of NMR methods. While detailed math and quantum mechanics dealing with NMR theory have been addressed in several well-known NMR volumes, chapter two of this volume illustrates the fundamental principles and concepts of NMR spectroscopy in a more descriptive manner. Topics such as instrument setup, data acquisition, and data processing using a variety of offline software are discussed. Chapters further discuss several routine stategies for preparing samples, especially for macromolecules and complexes. The target market for such a volume includes researchers in the field of biochemistry, chemistry, structural biology and biophysics.

Download or read book NMR of Macromolecules written by G. C. K. (Ed.). ROBERTS and published by . This book was released on 1993 with total page 399 pages. Available in PDF, EPUB and Kindle. Book excerpt: List of contributors; Nomenclature and abbreviations; Introduction; Sample preparation; Instrumentation and pulse sequences; Resonance assignment strategies for small proteins; Stable isotope labelling and resonance assigments in larger proteins; Effects of chemical exchange on NMR spectra; NMR studies of protein-ligand interactions; NMR of nucleic acids: from spectrum to structure; H NMR studies of oligosaccharides; structure determination from NMR data I. analysis of NMR data; Structure determination from NMR data II. computational approaches; Appendix; Index.

Download or read book NMR in Biological Systems written by K.V.R. Chary and published by Springer Science & Business Media. This book was released on 2008-04-01 with total page 552 pages. Available in PDF, EPUB and Kindle. Book excerpt: During teaching NMR to students and researchers, we felt the need for a text-book which can cover modern trends in the application of NMR to biological systems. This book covers the entire area of NMR in Biological Sciences (Biomolecules, cells and tissues, animals, plants and drug design). As well as being useful to researchers, this is an excellent book for teaching a course on NMR in Biological Systems.

Download or read book Studies of Structure Hydration and Dynamics of Biological Macromolecules by Use of NMR Spectroscopy written by and published by . This book was released on 2007 with total page 171 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nuclear magnetic resonance (NMR) has emerged as a powerful tool for studying biological macromolecules in aqueous solution. This dissertation describes the efforts to study the structure, hydration and dynamics of biological macromolecules by solution state NMR spectroscopy. There are three projects covered by the dissertation: (i) polypeptide hydration studied by NMR in supercooled water; (ii) structure determination of protein AQ-1857 by NMR spectroscopy and (iii) base flipping mechanism of M. Hha I DNA methyltransferase. Chapter 1 gives brief introduction of NMR applications in biological sciences. Chapter 2 describes the first investigation of polypeptide hydration in supercooled water at temperatures down to -18, which allowed identification of the zero crossing points of intermolecular NOEs between hydration water protons and peptide protons. The experimental results were then fitted to spatial motional models to calculate the translational diffusion coefficients of different hydration water molecules. Chapter 3 presents data collection, spectral analysis and structure calculation of the NESGC target protein AQ-1857, pursued in the context of high-throughput structure determination efforts for structural genomics. Chapter 4, the centerpiece of this dissertation, describes the tremendous efforts in investigating the base flipping mechanism of M. Hha I DNA methyltransferase, including enzymatic synthesis of a partially 13 C, 15 N labeled cognate DNA duplex and the NMR studies on the MTase-DNA complexes. The ample NMR information from chemical shifts, line widths and peak intensities, and imino proton exchange rates provided new insights for understanding the base flipping process.

Download or read book Pocket Guide to Biomolecular NMR written by Michaeleen Doucleff and published by Springer Science & Business Media. This book was released on 2011-01-03 with total page 166 pages. Available in PDF, EPUB and Kindle. Book excerpt: Steering clear of quantum mechanics and product operators, "Pocket Guide to Biomolecular NMR" uses intuitive, concrete analogies to explain the theory required to understand NMR studies on the structure and dynamics of biological macromolecules. For example, instead of explaining nuclear spin with angular momentum equations or Hamiltonians, the books describes nuclei as "bells" in a choir, ringing at specific frequencies depending on the atom type and their surrounding electromagnetic environment.This simple bell analogy, which is employed throughout the book, has never been used to explain NMR and makes it surprisingly easy to learn complex, bewildering NMR concepts, such as dipole-dipole coupling and CPMG pulse sequences. Other topics covered include the basics of multi-dimensional NMR, relaxation theory, and Model Free analysis. The small size and fast pace of “Pocket Guide to Biomolecular NMR” makes the book a perfect companion to traditional biophysics and biochemistry textbooks, but the book's unique perspective will provide even seasoned spectroscopists with new insights and handy “thought” short-cuts.

Download or read book Protein NMR for the Millennium written by N. Rama Krishna and published by Springer Science & Business Media. This book was released on 2006-04-11 with total page 345 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protein NMR for the Millennium is the third volume in a special thematic series devoted to the latest developments in protein NMR under the Biological Magnetic Resonance umbrella. This book is divided into three major sections dealing with significant recent advances in the study of large proteins in solution and solid state, structure refinement, and screening of bioactive ligands. Key Features: TROSY, Segmental isotope labeling of proteins, Hydrogen bond scalar couplings, Structure refinement based on residual dipolar couplings, Written by the world's foremost experts who have provided broad leadership in advancing the protein NMR field.

Download or read book Conformation of Biological Molecules written by G. Govil and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 226 pages. Available in PDF, EPUB and Kindle. Book excerpt: The determination of the three-dimensional structure of a biological molecule is the starting point in the understanding of molecular mechanisms involved in its complex biochemical reactions. The molecular architecture of multimolecular systems such as membranes and chromosomes provides the key to the fascinating field of molecular biology. Stereochemical details of biological macromolecules and their interactions with pharmacological agents form the basis for drug design. Naturally, the study of the structure and function of biological molecules has aroused tremendous interest and investigations in this area are being carried out in a large number of laboratories. The techniques used for this purpose include both experimental methods (X-ray and neutron diffraction measurements, study of NMR, ESR, vibrational and electronic spectra, ORD, CD and dipole moment measurements, biochemical modifications etc. ) and the oretical methods (quantum mechanical and classical potential energy calculations, Monte Carlo simulations and molecular graphics). F or several years now, X-ray diffraction [1] has served as our only source of infor mation on the three-dimensional arrangements of atoms in biopolymers. Fiber-diffrac tion of DNA led to the proposal of the DNA double helix. Fibers of long~hain polymers show ordering in the direction of the fibre-axis but not in the transverse plane. Accurate estimates of the dimensions of helical structures can be made using techniques on the basis of which models of biopolymers can be constructed.

Download or read book NMR of Polymers written by Frank A. Bovey and published by Academic Press. This book was released on 1996-11-19 with total page 471 pages. Available in PDF, EPUB and Kindle. Book excerpt: NMR has made important contributions to our understanding of structure–property relationships in polymers. This book provides an up-to-date and comprehensive overview of the fundamentals of NMR, with applications of multidimensional NMR and the new solution and solid-state methods in polymer science. NMR of Polymers is written by leading authorities for graduate students and professionals in academia and industry. Provides comprehensive overview of NMR in Polymer Science Covers multidimensional NMR Includes new solution and solid state methods Addresses chain conformation and dynamics

Download or read book NMR of Macromolecules written by Gordon Carl Kenmure Roberts and published by Oxford University Press, USA. This book was released on 1993 with total page 486 pages. Available in PDF, EPUB and Kindle. Book excerpt: Following the enormous increase in the use of nuclear magnetic resonance to study the conformations and interactions of biological macromolecules, this book provides detailed guidance on how to choose the most appropriate protocol to obtain the required information, how to carry out theexperiment, and how to analyze the resulting spectra. Graduate students and post-doctoral researchers in biochemistry, biophysics, chemistry, and other disciplines who use NMR to study biological macromolecules will find this exemplary volume one of the few genuinely practical books on thesubject.

Download or read book Biological NMR Spectroscopy written by John L. Markley and published by Oxford University Press. This book was released on 1997-01-30 with total page 375 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents a critical assessment of progress on the use of nuclear magnetic resonance spectroscopy to determine the structure of proteins, including brief reviews of the history of the field along with coverage of current clinical and in vivo applications. The book, in honor of Oleg Jardetsky, one of the pioneers of the field, is edited by two of the most highly respected investigators using NMR, and features contributions by most of the leading workers in the field. It will be valued as a landmark publication that presents the state-of-the-art perspectives regarding one of today's most important technologies.