Download or read book New Approach in Fabrication of Solid State Nanopore for Bio Sensing Applications written by Wing Hei Harold Kwok and published by . This book was released on 2015 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Solid State Nanopores written by Jean-Pierre Leburton and published by Springer Nature. This book was released on 2023-09-30 with total page 234 pages. Available in PDF, EPUB and Kindle. Book excerpt: This contributed volume provides an overview of the recent advances in solid-state nanopore technology, featuring contributions by leading experts in the field. It discusses several aspects of solid-state nanopores, covering their fabrication as well as multiple biosensing applications. It successfully bridges the gap between various scientific and engineering disciplines and highlights the progress made in this area. This title is a useful tool for acquiring basic knowledge of this field and following recent progress. It is a valuable contribution to the area of nanopore biosensing and is of interest to graduate students, postdocs, or senior researchers working in the fields of physical chemistry, biochemistry, bio- and electrical engineering, and biophysics.
Download or read book Nanopore Biosensors for Molecular Detection and Analysis written by Muhammad Usman Raza and published by . This book was released on 2019 with total page 109 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nanoscale fabrication techniques have led the march towards a new class of biosensors that were not possible to build in the past due to technological limitations. These molecular nanosensors can have biochemical, electromagnetic, acoustic, optical or electrophoretic detection modalities. One of these nanosensors, is solid state nanopores which use resistive pulse sensing in nanometer apertures in thin dielectric membranes. Solid state nanopores have been fabricated using cutting edge fabrication techniques to detect and analyze nanoscale biomolecules such as DNA, proteins, miRNA, viruses etc. These biosensors can be used as electronic sensors for biomarkers of certain diseases such as cancer and can form effective tools for early detection which can eventually save lives. Solid state nanopores have also been used as a novel way for cheap DNA sequencing. In the case for early detection of cancer, molecular biology plays a critical role and solid state nanopores can be used to study that and provide effective ways for the effect. In this dissertation we work on solid state nanopores and try to improve them using the cutting-edge fabrication techniques and optical modalities to make them ultrasensitive biomarker detection and multiplexed sensing biosensors. In the first project, we use solid state nanopores in silicon nitride membranes to detect epidermal growth factor receptor (EGFR) protein which is overexpressed in many cancers and is a cancer biomarker. We use an RNA Aptamer to selectively bind to the EGFR which increases the selectivity for this cancer biomarker through solid state nanopores. In the second project, we simulate two adjacent nanopores on a single membrane in the quest to find the adjacent nanopore distance for reduced electronic crosstalk. The optical parameters derived from the simulations can be used as a blue print for fabrication of nanopore arrays on single membranes to increase the throughput and self-referencing capability for noise cancellation. The final project reinvents the solid state nanopore with the inclusion of an optical plasmonic trap cavity at the mouth of the nanopore. We are the first ones to report this type of nanopore device. This novel sensor provides dual mode optical and electrical sensing capability. In addition, the localized plasmon field can trap the nanoparticles for up to 7 seconds at the mouth of the nanopore, which was previously not possible due to the electrophoretic translocation. It is shown that nanoparticles oscillate in the trap, which results in high frequency electrical noise that is synchronous to the mass loading of the plasmonic cavity.
Download or read book Engineered Nanopores for Bioanalytical Applications written by Joshua B. Edel and published by William Andrew. This book was released on 2013-03-19 with total page 189 pages. Available in PDF, EPUB and Kindle. Book excerpt: Engineered Nanopores for Bioanalytical Applications is the first book to focus primarily on practical analytical applications of nanopore development. These nanoscale analytical techniques have exciting potential because they can be used in applications such as DNA sequencing, DNA fragment sizing, DNA/protein binding, and protein/protein binding. This book provides a solid professional reference on nanopores for readers in academia, industry and engineering and biomedical fields. In addition, the book describes the instrumentation, fabrication, and experimental methods necessary to carry out nanopore-based experiments for developing new devices. Includes application case studies for detection, identification and analysis of biomolecules and related functional nanomaterials Introduces the techniques of manufacturing solid state materials with functional nanopores Explains the use of nanopores in DNA sequencing and the wider range of applications from environmental monitoring to forensics
Download or read book Label Free Biosensing written by Michael J. Schöning and published by Springer. This book was released on 2018-07-20 with total page 485 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume summarizes the state-of-the-art technologies, key advances and future trends in the field of label-free biosensing. It provides detailed insights into the different types of solid-state, label-free biosensors, their underlying transducer principles, advanced materials utilized, device-fabrication techniques and various applications. The book offers graduate students, academic researchers, and industry professionals a comprehensive source of information on all facets of label-free biosensing and the future trends in this flourishing field. Highlights of the subjects covered include label-free biosensing with: · semiconductor field-effect devices such as nanomaterial-modified capacitive electrolyte-insulator-semiconductor structures, silicon nanowire transistors, III-nitride semiconductor devices and light-addressable potentiometric sensors · impedimetric biosensors using planar and 3D electrodes · nanocavity and solid-state nanopore devices · carbon nanotube and graphene/graphene oxide biosensors · electrochemical biosensors using molecularly imprinted polymers · biomimetic sensors based on acoustic signal transduction · enzyme logic systems and digital biosensors based on the biocomputing concept · heat-transfer as a novel transducer principle · ultrasensitive surface plasmon resonance biosensors · magnetic biosensors and magnetic imaging devices
Download or read book Nanopores written by Samir M. Iqbal and published by Springer Science & Business Media. This book was released on 2011-07-12 with total page 377 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nanopores are nanometer scale holes formed naturally by proteins or cells, and can be used for a variety of applications, including sequencing DNA and detecting anthrax. They can be integrated into artificially constructed encapsulated cells of silicon wafers while allowing small molecules like oxygen, glucose and insulin to pass, while keeping out large system molecules. "Nanopores: Sensing and Fundamental Biological Interactions" examines the emerging research directions surrounding nanopores such as genome sequencing and early disease detection using biomarker identification. Covering the applications of nanopores in genetics, proteomics, drug discovery, early disease detection and detection of emerging environmental threats, it is a must-have book for biomedicalengineers and research scientists.
Download or read book Carbon Based Nanosensor Technology written by Christine Kranz and published by Springer. This book was released on 2019-02-13 with total page 308 pages. Available in PDF, EPUB and Kindle. Book excerpt: Carbon nanomaterials have gained relevance in chem/bio sensing applications owing to their unique chemical, mechanical, electrical, and thermal characteristics. Written by leading experts in the field, this book discusses selected, state-of-the art carbon-based nanomaterials, including nanodiamonds, graphene nanodots, carbon nanopores, and nanocellulose. It presents examples of chem/bio sensing applications ranging from biomedical studies, such as DNA sequencing and neurotransmitter sensing, to heavy-metal detection in environmental monitoring scenarios, and reviews the unique properties of carbon-based nanomaterials with respect to targeted sensing applications. Further, it highlights exciting future applications. Providing comprehensive information for practitioners and scientists working in the field of carbon nanomaterial technologies and their application, it is also a valuable resource for advanced students of analytical chemistry, biochemistry, electrochemistry, materials science, and micro-/nanotechnology and -sensing.
Download or read book Nano Bio Sensing written by Sandro Carrara and published by Springer Science & Business Media. This book was released on 2010-11-01 with total page 254 pages. Available in PDF, EPUB and Kindle. Book excerpt: The application of circuits and systems and engineering principles to problems in the medicine has led to the emergence of biomedical circuits and systems as an exciting and rapidly growing area of research. Nanotechnology provides new nano-structured materials with amazing properties. The properties offered by nanomaterials can be applied to develop advanced instrumentation for biomedical diagnostics and personalized therapy, as well as bio-sensing in the environment. Biotechnology provides new biochemical materials with novel properties to be applied to develop new performances in sensing techniques. These advancements in Nano- and Bio- technologies will lead to new concepts and applications for nano-bio-sensing systems. This book offers an invaluable reference to the state-of-the-art applications of nano-bio-sensing. It brings together expertise of researchers from the fields of nano-electronics and bio-technology, providing multidisciplinary content from nano-structures fabrication to bio-sensing applications.
Download or read book Nanopores for Bioanalytical Applications written by Joshua Edel and published by Royal Society of Chemistry. This book was released on 2012-09-05 with total page 121 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bioanalytical applications in nanopores are an exciting field of research which have seen rapid development over the last ten plus years. This book focuses uniquely on this research area providing an insight from the world leading experts in the area. Based on the proceedings of the 1st International Conference on Nanopores for Bioanalytical Applications (Lanzarote, 2012), it will provide a snapshot of this exciting field for the nanopores community. It describes the latest experimental and fabrication protocols necessary to carry out nanopore-based experiments and covers a wide variety of topics. These include: biological pores, solid state pores, hybrid structures, fundamental aspects, work on the underlying (bio)physics and applications in biosensing and DNA sequencing. The multidisciplinary approach to this topic makes the book suitable for readers in both academia and industry.
Download or read book Solid State Nanopores written by Kyle Briggs and published by . This book was released on 2018 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The work presented in this thesis is divided loosely into three main areas of interest: development of a novel method of solid-state nanopore fabrication; applications of this method to some of the open problems in the field; and analysis of nanopore data. The first of these occupies the majority of the research presented in this thesis, covering research dedicated to the development and characterization of a novel method of solid-state nanopore fabrication which achieves nanometer scale control over matter using simple and low cost circuitry. Termed controlled breakdown (CBD), this technique is in the process of revolutionizing the field of nanopore research, and in the few short years I have been part of its development it has seen adoption in nanopore labs across the globe, both academic and industrial. Due to the simple nature of CBD, this technique also enables novel applications of nanopores in device architectures that were inaccessible to the expensive and inflexible methods used previously. The second part of this thesis takes advantage of the unique opportunities presented by CBD to develop a device architecture comprising two nanopores in series. This nanodevice tackles one of the main problems standing between nanopores and the promise of cheap genomic analysis: control of the motion and conformation of the polymer both prior to and during translocation through the pore. Finally, because the field of nanopore research is still relatively young, very few tools are available which provide high-quality analysis of nanopore data. The last part of this thesis is dedicated to a thorough discussion of the complexities involved in analysing nanopore signals, as well as the development of several tools which directly address this knowledge gap.
Download or read book Fabrication and Characterization of Thinner Solid state Nanopores written by Denis Forbi Tita and published by . This book was released on 2012 with total page 118 pages. Available in PDF, EPUB and Kindle. Book excerpt: Solid State nanopores that are fabricated by the ion beam sculpting process and electron beam drilling have shown great promise as a sensing device for DNA and protein molecules. Even though biological pores such as the alpha-Haemolysin have been in use for quite some time, the use of solid state Nanopores in single biomolecule detection has been on the rise since the mid 1990s. Solid State nanopores have an advantage over biological pores in that they are more robust, stable, and can be sculpted to any desired size for use in translocation experiments. One of the major challenges in Nanopore fabrication by ion beam sculpting has been limited by the user's ability to control the closure rate of pores in the fabrication process. Another challenge in nanopore sensing is the resolution limitation due to the thickness of the pore. This is because most of the nanopores fabricated by the ion beam sculpting method are often thicker than they should. This thesis will focus on the modification of nanopore fabrication using the ion beam sculpting system at the University of Arkansas by first baking the samples in vacuum under specified temperature conditions. Baking the samples will give the user better control over pore closure. This Thesis will also focus on thinning the sculpted pores by Reactive Ion Etching in an attempt to increase its resolution for single biomecule translocation experiments.
Download or read book Trends in Biosensing Research written by Fred Lisdat and published by Springer Nature. This book was released on with total page 448 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Solid state Nanopore Sensors for Nucleic Acid Analysis written by Bala Murali K. Venkatesan and published by . This book was released on 2011 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Nanopore DNA analysis is an emerging technique that involves electrophoretically driving DNA molecules through a nano-scale pore in solution and monitoring the corresponding change in ionic pore current. This versatile approach permits the label-free, amplification-free analysis of charged polymers (single stranded DNA, double stranded DNA and RNA) ranging in length from single nucleotides to kilobase long genomic DNA fragments with subnanometer resolution. Recent advances in nanopores suggest that this low-cost, highly scalable technology could lend itself to the development of third generation DNA sequencing technologies, promising rapid and reliable sequencing of the human diploid genome for under $1000. Here, we report the development of versatile, nano-manufactured Al2O3 solid-state nanopores and nanopore arrays for rapid, label-free, single-molecule detection and analysis of DNA and protein. This nano-scale technology has proven to be reliable, affordable, and mass producible, and allows for integration with VLSI processes. A detailed characterization of nanopore performance in terms of electrical noise, mechanical robustness and materials analysis is provided, and the functionality of this technology in experimental DNA biophysics is explored. A framework for the application of this technology to medical diagnostics and sequencing is also presented. Specifically, studies involved the detection of DNA-protein complexes, a viable strategy in screening methylation patterns in panels of genes for early cancer detection, and the creation of lipid bilayer coated nanopore sensors, useful in creating hybrid biological/solid-state nanopores for DNA sequencing applications. The concept of a gated nanopore is also presented with preliminary results. The fabrication of this novel system has been enabled by the recent discovery of graphene, a highly versatile material with remarkable electrical and mechanical properties. Direct modulation of the nanopore conductance was observed through the application of potentials to the graphene gate. These exciting results suggest this technology could potentially be useful in slowing down or trapping a DNA molecule in the pore, thereby enabling solid-state nanopore sequencing.
Download or read book Selected Topics In Nanomedicine written by Thomas Ming Swi Chang and published by World Scientific. This book was released on 2013-09-16 with total page 599 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nanomedicine consists of the use of nanotechnology and nanobiotechnology in medicine. There have been extensive developments in the area of nanomedicine. The scope of this book is first to discuss the origin of nanomedicine. Following this, instead of a general overview of the whole area, 24 chapters on selected topics of important areas are described in detail. Authors are selected from around the world to give a representative and international view of the activities in the area of nanomedicine.
Download or read book Nanomaterials written by Suvardhan Kanchi and published by John Wiley & Sons. This book was released on 2018-05-29 with total page 291 pages. Available in PDF, EPUB and Kindle. Book excerpt: The evolution in the nanotechnology world clearly signifies a need for a broader understanding of the subject and this book will contribute to the effort. Nanostructure science and technology is a broad and interdisciplinary area of research and development that has been growing explosively in the past decades. The contents of this book include mainly the fundamentals of nanoparticles, state-of-the-art in synthesis and characterization of nanomaterials, as well the influence of nanomaterials on the analytical systems (macro to micro & lab-on-a-chip) for biomedical, environmental and engineering applications. This book seeks to broaden the understanding of modern developments in nanomaterials and comprises excellent contributions from subject matter experts working on most aspects of nanomaterials and nanotechnology.
Download or read book Handbook of Nanophysics written by Klaus D. Sattler and published by CRC Press. This book was released on 2010-09-17 with total page 888 pages. Available in PDF, EPUB and Kindle. Book excerpt: The tools of nanodiagnostics, nanotherapy, and nanorobotics are expected to revolutionize the future of medicine, leading to presymptomatic diagnosis of disease, highly effective targeted treatment therapy, and minimum side effects. Handbook of Nanophysics: Nanomedicine and Nanorobotics presents an up-to-date overview of the application of nan
Download or read book Chemically Modified Nanopores and Nanochannels written by Mario Tagliazucchi and published by William Andrew. This book was released on 2016-08-29 with total page 227 pages. Available in PDF, EPUB and Kindle. Book excerpt: Chemically Modified Nanopores and Nanochannels is devoted to chemically modified nanopores and nanochannels, and covers the fundamentals of transport in chemically modified systems, an account of the different preparation and characterization techniques of chemically modified nanopores, their applications, and case studies. The book is designed for materials and biomaterials scientists, biomedical engineers, chemists, and chemical engineers who are interested in designing and utilizing processes to synthesize, modify, characterize, use, and model nanopores. The strong chemical focus of the book differentiates it from other books published on nanopores, which traditionally focus either on physics, biophysics, and nanofabrication (solid-state nanopores) or biophysics and biology (biological ion channels and pores). Explains how the chemical modification of nanopores and nanochannels can be used in filtration, membranes, and sensing Provides advanced coverage of novel synthetic applications Focuses on the latest developments in nanopore and nanochannel engineering Presents an account of the different preparation and characterization techniques of chemically modified nanopores, their applications, and case studies
