Download or read book Mutational Analysis of the Infected Cell Protein 0 ICP0 Promoter of Herpes Simplex Virus Type 1 HSV 1 written by David J. Davido and published by . This book was released on 1996 with total page 264 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Human Herpesviruses written by Ann Arvin and published by Cambridge University Press. This book was released on 2007-08-16 with total page 1325 pages. Available in PDF, EPUB and Kindle. Book excerpt: This comprehensive account of the human herpesviruses provides an encyclopedic overview of their basic virology and clinical manifestations. This group of viruses includes human simplex type 1 and 2, Epstein–Barr virus, Kaposi's Sarcoma-associated herpesvirus, cytomegalovirus, HHV6A, 6B and 7, and varicella-zoster virus. The viral diseases and cancers they cause are significant and often recurrent. Their prevalence in the developed world accounts for a major burden of disease, and as a result there is a great deal of research into the pathophysiology of infection and immunobiology. Another important area covered within this volume concerns antiviral therapy and the development of vaccines. All these aspects are covered in depth, both scientifically and in terms of clinical guidelines for patient care. The text is illustrated generously throughout and is fully referenced to the latest research and developments.
Download or read book Gene Regulation and Function of Icp0 in Herpes Simplex Virus Infected Cells written by Mingyu Liu and published by . This book was released on 2011-09-09 with total page 124 pages. Available in PDF, EPUB and Kindle. Book excerpt: Herpes simplex virus (HSV) is a clinically important virus, whose life cycle alternates between productive replication and latency. Infected cell protein 0 (ICP0) is generally believed to play a key role in determining the outcome of HSV infections. Synthesis of ICP0 promotes the productive replication of HSV, whereas absence of ICP0 renders HSV prone to establish latent infections. In the first part of the dissertation, I attempt to address the question how is ICP0 gene regulated. To tackle this question, we constructed recombinant HSV that encodes GFP-tagged ICP0 so that the regulation of ICP0 gene can be visualized in real time. Using this reagent, we found that ICP0 gene was subject to potent repression immediately following infection. Surprisingly, HSV's major transcriptional regulator, ICP4, was necessary and sufficient to repress ICP0 gene, and did so in an ICP4-binding-site dependent manner. Synthesis of ICP0 alleviated the ICP4-dependent repression of ICP0 gene. ICP4 co-immunoprecipitated with FLAG-tagged ICP0, thus, a physical interaction between ICP0 and ICP4 likely explains how ICP0 antagonizes ICP4's capacity to silence the ICP0 gene. Therefore, our findings suggest that ICP0 gene is differentially regulated by virus-encoded repressor ICP4 and virus-encoded antirepressor ICP0. In the second part of the dissertation, I attempt to address the question what function does ICP0 assume. Since the discovery of ICP0, the nuclear function of ICP0 has been the focal point of studies, whereas the cytoplasmic function of ICP0 is unknown. While pursuing our first study, we unexpectedly found that GFP-tagged ICP0 was predominantly localized to the cytoplasm during infections. Taking advantage of live-cell imaging, we found that ICP0 translocated from nucleus to cytoplasm during early phase of HSV infections, where it bundled and dispersed microtubules. Synthesis of ICP0 was proved to be necessary and sufficient to dismantle microtubules in HSV-infected and transfected cells. Therefore, our findings suggest ICP0 might play a previously unrecognized role in the cytoplasm through dismantling microtubule networks of the host cells. Furthermore, our study represents the first report showing a virus-encoded E3 ligase disrupts host cell microtubule networks, thus suggests a general function of many other viral E3 ligases.
Download or read book The Activation of a Viral Late Gene by Herpes Simplex Virus Type 1 ICP27 written by Keith Dwight Perkins and published by . This book was released on 2003 with total page 414 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book A Structural and Functional Analysis of Herpes Simplex Virus Type 1 Glycoprotein L an Essential Component of the Viral Fusogenic Complex written by Michael J. Novotny and published by . This book was released on 1996 with total page 456 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Chromatin and Coactivators in Herpes Simplex Virus Type 1 Gene Regulation written by Sebla Bulent Kutluay and published by . This book was released on 2009 with total page 436 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Mutational and Molecular Genetic Analysis of the UL 38 Promoter of Herpes Simplex Virus Type 1 written by John Frederick Guzowski and published by . This book was released on 1994 with total page 412 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Regulation and Export of ICP27 During Herpes Simplex Virus Type 1 Lytic Infection written by Anna Karin Strain and published by . This book was released on 2007 with total page 368 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Herpes Simplex Virus Type I HSV 1 induced Cell Fusion written by Tracy L. Terry-Allison and published by . This book was released on 1999 with total page 356 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Analysis of the Cellular Heat Shock Response and Machinery During Herpes Simplex Virus Type I Infection written by Christine Marie Livingston and published by . This book was released on 2010 with total page 566 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Proteasome dependent Entry of Herpes Simplex Virus written by Mark Delboy and published by . This book was released on 2010 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Herpes simplex virus entry into cells is a multistep process that engages the host cell machinery. The proteasome is a large, ATP-dependent, multisubunit protease that plays a critical role in the maintenance of cell homeostasis. A battery of assays were used to demonstrate that proteasome inhibitors blocked an early step in herpes simplex virus entry that occurred after capsid penetration into the cytosol but prior to capsid arrival at the nuclear periphery. Proteasome-dependent viral entry was not reliant on host or viral protein synthesis. MG132, a peptide aldehyde that competitively inhibits the degradative activity of the proteasome, had a reversible inhibitory effect on herpes simplex virus capsid transport. Herpes simplex virus can use endocytic or nonendocytic pathways to enter cells. These distinct entry routes were both dependent on proteasome-mediated proteolysis. In addition, herpes simplex virus successfully entered cells in the absence of a functional host ubiquitin-activating enzyme, suggesting that viral entry is ubiquitin independent. Herpes simplex virus immediate-early protein ICP0 is a multifunctional regulator of herpes simplex virus infection. Late in infection ICP0 interacts dynamically with cellular proteasomes. ICP0 has a RING finger domain with E3 ubiquitin ligase activity that is necessary for its IE functions. The fundamental and functional properties of ICP0 that is present in the virion tegument layer have not been well characterized. For these reasons, I sought to characterize tegument ICP0 and determine the role of tegument ICP0 during proteasome-dependent entry of herpes simplex virus. Protein compositions of wild-type and ICP0 null virions were similar, suggesting that the absence of ICP0 does not grossly impair virion assembly. Virions with mutations in the RING finger domain contained greatly reduced levels of tegument ICP0, suggesting that the domain influences the incorporation of ICP0. Virion ICP0 was resistant to removal by detergent and salt and was associated with capsids, features common to inner tegument proteins. ICP0 mutations that resulted in the absence of ICP0 in the tegument layer, allow herpes simplex virus to enter cells independently of the proteasome activity. I propose that proteasomal degradation of virion and/or host proteins is regulated by ICP0 to allow for efficient delivery of incoming herpes simplex virus capsids to the nucleus.
Download or read book Cell Biology of Herpes Viruses written by Klaus Osterrieder and published by Springer. This book was released on 2017-05-20 with total page 226 pages. Available in PDF, EPUB and Kindle. Book excerpt: Herpes viruses are widely distributed in nature, causing disease in organisms as diverse as bivalves and primates, including humans. Each virus appears to have established a long-standing relationship with its host, and the viruses have the ability to manipulate and control the metabolism of host cells, as well as innate and adaptive antiviral immune responses. Herpes viruses maintain themselves within hosts in a latent state resulting in virus persistence for years – usually for the life span of the hosts. Herpes viruses comprise a large number of pathogens with diverse cellular targets and biological consequences of infection. What they have in common is their structure and the fact that they establish a dormant (latent) infection in their hosts that usually persists for life. The reviews here will highlight the general principles of herpes virus infection, with equal attention to overall principle and important difference. Also, the cell type- and life-style dependent differences in the establishment and maintenance of virus persistence will be covered.
Download or read book A Guide to Human Gene Therapy written by Roland W. Herzog and published by World Scientific. This book was released on 2010 with total page 415 pages. Available in PDF, EPUB and Kindle. Book excerpt: 1. Non-viral gene therapy / Sean M. Sullivan -- 2. Adenoviral vectors / Stuart A. Nicklin and Andrew H. Baker -- 3. Retroviral vectors and integration analysis / Cynthia C. Bartholomae [und weitere] -- 4. Lentiviral vectors / Janka Matrai, Marinee K.L. Chuah and Thierry VandenDriessche -- 5. Herpes simplex virus vectors / William F. Goins [und weitere] -- 6. Adeno-Associated Viral (AAV) vectors / Nicholas Muzyczka -- 7. Regulatory RNA in gene therapy / Alfred. S. Lewin -- 8. DNA integrating vectors (Transposon, Integrase) / Lauren E. Woodard and Michele P. Calos -- 9. Homologous recombination and targeted gene modification for gene therapy / Matthew Porteus -- 10. Gene switches for pre-clinical studies in gene therapy / Caroline Le Guiner [und weitere] -- 11. Gene therapy for central nervous system disorders / Deborah Young and Patricia A. Lawlor -- 12. Gene therapy of hemoglobinopathies / Angela E. Rivers and Arun Srivastava -- 13. Gene therapy for primary immunodeficiencies / Aisha Sauer, Barbara Cassani and Alessandro Aiuti -- 14. Gene therapy for hemophilia / David Markusic, Babak Moghimi and Roland Herzog -- 15. Gene therapy for obesity and diabetes / Sergei Zolotukhin and Clive H. Wasserfall -- 16. Gene therapy for Duchenne muscular dystrophy / Takashi Okada and Shin'ichi Takeda -- 17. Cancer gene therapy / Kirsten A.K. Weigel-Van Aken -- 18. Gene therapy for autoimmune disorders / Daniel F. Gaddy, Melanie A. Ruffner and Paul D. Robbins -- 19. Gene therapy for inherited metabolic storage diseases / Cathryn Mah -- 20. Retinal diseases / Shannon E. Boye, Sanford L. Boye and William W. Hauswirth -- 21. A brief guide to gene therapy treatments for pulmonary diseases / Ashley T. Martino, Christian Mueller and Terence R. Flotte -- 22. Cardiovascular disease / Darin J. Falk, Cathryn S. Mah and Barry J. Byrne
Download or read book Human Herpesviruses written by Yasushi Kawaguchi and published by Springer. This book was released on 2018-06-12 with total page 498 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book introduces and reviews several topics for each human herpesvirus. One of the most important features of the book is that it covers aspects of both basic research and clinical medicine. Herpesviridae, a family of double-strand DNA viruses, has unique biological features by which these viruses establish latency after primary infection and reactivate in later life. Nine human herpesviruses are known so far, and each of them causes a variety of diseases in both primary infection and reactivation. Since the discovery of each human herpesvirus, an abundance of findings related to them has accumulated in basic research and clinical medicine. However, the vast majority of biological features is still masked in mystery. Furthermore, a strategy of treatment and prevention has not yet been established for most human herpesviruses. A wide range of readers will be interested in this volume with its treatment of problematic points and latest findings in the field.
Download or read book The Nucleolus written by Mark O. J. Olson and published by Springer Science & Business Media. This book was released on 2011-09-15 with total page 434 pages. Available in PDF, EPUB and Kindle. Book excerpt: Within the past two decades, extraordinary new functions for the nucleolus have begun to appear, giving the field a new vitality and generating renewed excitement and interest. These new discoveries include both newly-discovered functions and aspects of its conventional role. The Nucleolus is divided into three parts: nucleolar structure and organization, the role of the nucleolus in ribosome biogenesis, and novel functions of the nucleolus.
Download or read book American Doctoral Dissertations written by and published by . This book was released on 1996 with total page 872 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Applied Basic Science for Basic Surgical Training E Book written by Andrew T Raftery and published by Elsevier Health Sciences. This book was released on 2011-12-22 with total page 660 pages. Available in PDF, EPUB and Kindle. Book excerpt: This title is directed primarily towards health care professionals outside of the United States. It has been written to encompass the basic anatomy, physiology and pathology required by the syllabus of the UK Royal Colleges and the Intercollegiate Surgical Curriculum Project. For this Second Edition many of the chapters have been updated, especially the chapters on immunology, basic microbiology, the endocrine and locomotor systems and the breast. An attempt has been made throughout to indicate the clinical relevance of the facts and the reason for learning them. There are several new contributors to the author team, all of whom are experts in their field and many of them are, or have been, experienced examiners at the various UK Royal Colleges. Brings together three basic sciences in one book and presents them in an integrated format. Presents the sciences at the right level for surgical trainees. Written in a straightforward and readable style. Thoroughly revised to take account of latest changes in basic surgical training, especially the chapters on immunology, basic microbiology, the endocrine and locomotor systems and the breast
