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Book Multiplexed Imaging

    Book Details:
  • Author : Eli Zamir
  • Publisher : Humana
  • Release : 2021-08-31
  • ISBN : 9781071615928
  • Pages : 343 pages

Download or read book Multiplexed Imaging written by Eli Zamir and published by Humana. This book was released on 2021-08-31 with total page 343 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides a collection of state-of-the-art approaches addressing key aspects of multiplexed imaging. Chapters focus on labeling and imaging techniques for multiplexed imaging, as well as on the application of these techniques for the study of cells and tissues. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Multiplexed Imaging: Methods and Protocols aims to be helpful for researchers interested in implementing multiplexed imaging or in developing novel, cutting-edge multiplexed imaging approaches.

Book Multiplexed Imaging

    Book Details:
  • Author : Eli Zamir
  • Publisher : Humana
  • Release : 2022-08-15
  • ISBN : 9781071615959
  • Pages : 0 pages

Download or read book Multiplexed Imaging written by Eli Zamir and published by Humana. This book was released on 2022-08-15 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides a collection of state-of-the-art approaches addressing key aspects of multiplexed imaging. Chapters focus on labeling and imaging techniques for multiplexed imaging, as well as on the application of these techniques for the study of cells and tissues. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Multiplexed Imaging: Methods and Protocols aims to be helpful for researchers interested in implementing multiplexed imaging or in developing novel, cutting-edge multiplexed imaging approaches.

Book Massively Multiplexed Imaging Probes for Comprehensive Single cell Analysis

Download or read book Massively Multiplexed Imaging Probes for Comprehensive Single cell Analysis written by Sheldon J. J. Kwok and published by . This book was released on 2019 with total page 191 pages. Available in PDF, EPUB and Kindle. Book excerpt: Optical microscopy techniques are widely used to study cellular physiology in their native tissue environments. In particular, the use of fluorescent probes to tag different cell populations, subcellular compartments, specific proteins and nucleotide sequences has enabled examination of cellular phenotypes with increasingly sophisticated detail. Recent efforts to combine physiological imaging and single-cell molecular analysis seek complete understanding of cellular identity and function within complex tissues. Specific cells of interest can be selected and isolated from tissues for downstream molecular analyses using techniques such as laser capture micro-dissection, or cell tagging with photo-conversion. However, high-throughput, unbiased molecular profiling of every cell imaged within a tissue remains an elusive challenge. A fundamental obstacle in previous approaches is spectral overlap due to the relatively broad emission of typical fluorescent probes, which limits their capabilities for multiplexed tagging. The first part of this thesis describes methods for studying cellular physiology in mice at single-cell resolution using two-photon fluorescence microscopy. The second part of this thesis describes the development of a novel class of imaging probes, called laser particles, which rely on narrowband laser emission for massively multiplexed cell tagging. This work establishes laser particles as promising tools for comprehensive single-cell analyses.

Book Expansion Microscopy

    Book Details:
  • Author : Fei Chen (Ph. D.)
  • Publisher :
  • Release : 2017
  • ISBN :
  • Pages : 107 pages

Download or read book Expansion Microscopy written by Fei Chen (Ph. D.) and published by . This book was released on 2017 with total page 107 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microscopy has facilitated the discovery of many biological insights by optically magnifying small structures in cells and tissues. However, the resolution of optical microscopy is limited by the diffraction of light to ~200-300 nm, comparable or larger to the size of many subcellular structures. In this thesis, we describe a suite of tools based on a novel super-resolution microscopy approach called Expansion microscopy. Expansion microscopy (ExM) physically expands tissues so that the resolution of ordinary microscopes is increased -5 times by leveraging the swelling properties of polyelectrolyte hydrogels. Ordinary microscopes used with ExM are more accessible and faster than the specialized optical systems designed to image beyond the diffraction limit (e.g., STORM/PALM, STED, SIM), while yielding similar performance. Expanded tissues are also optically clear, allowing for unprecedented super-resolution imaging in thick tissues and facile reagent diffusion into the sample. We have since developed a variant of ExM, called protein retention ExM, in which proteins are directly anchored to the swellable gel using a commercially available cross-linking molecule. This strategy enables ExM of genetically encoded fluorescent proteins and commercial fluorescently labeled secondary antibodies. With these advancements, ExM can be carried out with purely commercial reagents and represents a simple extension of standard histological methods used to prepare samples for imaging. Furthermore, we have developed a variant of the ExM technology that enables RNA molecules to be directly linked to the ExM gel network via a small molecule linker and isotropic expansion. This technology, termed ExFISH, enables visualization of RNAs with nanoscale precision and single molecule resolution. We have demonstrated that the covalent anchoring of RNA also enables robust repeated washing and probe hybridization steps, opening the door to combinatorial multiplexing strategies. By leveraging these benefits, we have further developed in situ analysis tools which allow for highly multiplexed imaging of RNA identity and location with nanoscale precision in intact tissues. Taken together, these tools allow for spatially mapping molecular information onto cell types and tissue structures which could be invaluable for spatially complex biological processes such as brain function, cancer heterogeneity and organismal development.

Book Source Modulated Multiplexed Hyperspectral Imaging

Download or read book Source Modulated Multiplexed Hyperspectral Imaging written by Lee Streeter and published by . This book was released on 2009 with total page 147 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Book Multiplexed Hyperspectral Imaging and Spectrometry Using Spatial Light Modulators

Download or read book Multiplexed Hyperspectral Imaging and Spectrometry Using Spatial Light Modulators written by Richard A. DeVerse and published by . This book was released on 1999 with total page 474 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Book Image Processing Applications in Real Life

Download or read book Image Processing Applications in Real Life written by Houman Kamran Habibkhani and published by . This book was released on 2018 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Book Spectrally Resolved Detector Arrays for Multiplexed Biomedical Fluorescence Imaging

Download or read book Spectrally Resolved Detector Arrays for Multiplexed Biomedical Fluorescence Imaging written by Anna Siri Luthman and published by . This book was released on 2018 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: And Rationale -- Spectral Imaging Systems and Sensor Characterisations -- Wide-field fHSI with a Linescan SRDA -- A Multispectral Endoscope Based on SRDAs -- Conclusions and Outlook.

Book Multiplex Imaging and Random Arrays

Download or read book Multiplex Imaging and Random Arrays written by Christopher M. Brown and published by . This book was released on 1972 with total page 162 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Book A Demonstration of the Use of Walsh Functions for Multiplexed Imaging

Download or read book A Demonstration of the Use of Walsh Functions for Multiplexed Imaging written by Robert Hugh McKenzie and published by . This book was released on 1990 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Book Advanced Methods in Multiplexing Multi pinhole Imaging

Download or read book Advanced Methods in Multiplexing Multi pinhole Imaging written by Christian Lackas and published by . This book was released on 2006 with total page 176 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Book Spectrally Resolved Detector Arrays for Multiplexed Biomedical Fluorescence Imaging

Download or read book Spectrally Resolved Detector Arrays for Multiplexed Biomedical Fluorescence Imaging written by Anna Siri Luthman and published by Springer. This book was released on 2018-10-09 with total page 137 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book describes the design, development, characterisation and application of two novel fluorescence imaging instruments based on spectrally resolved detector arrays (SRDAs). The simplest SRDA is the standard colour camera, which integrates a Bayer filter array of red, green and blue colour filters to replicate the colour sensing capability of the human eye. The SRDAs used in this book contain many more colours, ranging from 16 to over 100 colour channels. Using these compact, robust and low-cost detectors for biomedical applications opens new avenues of exploration that were not possible before, in particular, the use of spectral imaging in endoscopy. The work presented shows for the first time that not only can this new type of camera be used for fluorescence imaging, but also that it is able to resolve signals from up to 7 different dyes – a level of multiplexing not previously achieved in tissue with such compact and robust equipment. Furthermore, it reports the application of a bimodal endoscope performing both reflectance and fluorescence imaging using these cameras in an ex vivo pig oesophagus model.

Book Highly multiplexed Imaging Analysis of Intra tumour Heterogeneity and Response to Immune Checkpoint Inhibitors in Colorectal Cancer

Download or read book Highly multiplexed Imaging Analysis of Intra tumour Heterogeneity and Response to Immune Checkpoint Inhibitors in Colorectal Cancer written by Michele Bortolomeazzi and published by . This book was released on 2022 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Book Advanced Multiplexing Techniques in Single Molecule Localisation Microscopy

Download or read book Advanced Multiplexing Techniques in Single Molecule Localisation Microscopy written by Nazar Oleksiievets and published by . This book was released on 2022 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: DNA point accumulation for imaging in nanoscale topography (DNA-PAINT) is a powerful super-resolution technique highly suitable for multi-target (multiplexing) bio-imaging applications. However, multiplexed imaging of cells is still challenging due to the dense and sticky environment inside a cell. This thesis presents several novel techniques for multiplexed imaging, with the main focus on the DNA-PAINT super-resolution technique. At first, I demonstrate multiplexed imaging by using three different single-domain antibodies (nanobodies). They have a three times smaller size than conventiona...