Download or read book Multiplex Microarrays for Diagnosis and Detection written by Bradley H. Garcia and published by . This book was released on 2008 with total page 100 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Detection of Highly Dangerous Pathogens written by Tanja Kostic and published by John Wiley & Sons. This book was released on 2009-04-29 with total page 184 pages. Available in PDF, EPUB and Kindle. Book excerpt: Written by leading experts in the field as part of an interdisciplinary pan-European research program funded by the EU, the results provided in this booklet provide a unique and comprehensive overview of how microarray technology can be used in safely tracking the most highly dangerous pathogens. A must-have for public health agencies focused on bioterrorism as well as all laboratories working with BSL3 and/or BSL 4 agents.
Download or read book Protein and Peptide based Microarrays for Multiplex Detection written by Navid Rabiee and published by IOP Publishing Limited. This book was released on 2020-10-07 with total page 70 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protein and Peptide-based Microarrays for Multiplex Detection looks at the aspects of protein and peptide-based microarrays for multiplex detection, based on Rabiee's Theory. A comprehensive guide for scientists, students and doctors.
Download or read book Microarrays in Diagnostics and Biomarker Development written by Bertrand Jordan and published by Springer Science & Business Media. This book was released on 2012-04-23 with total page 241 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microarray technology has made strong progress over the past decade, and there have also been significant changes in application areas, from nucleic acids to proteomics and from research to clinical applications. This book provides a comprehensive overview of microarrays in diagnostics and biomarker development, covering DNA, peptide, protein and tissue arrays. The focus is on entities that are in actual clinical use, or quite close, and on recent developments, such as peptide and aptamer arrays. A further topic is the miniaturisation towards “nanoarrays”, which is expected to have great potential in clinical applications. Relevant issues of bioinformatics and statistical analysis of array data are discussed in detail, as well as the barriers to the commercialisation of array-based tests and the vexing IP issues involved. Thus, the book should be very useful tor active array users as well as to newcomers seeking to make the best choice between different technologies.
Download or read book Microarrays in Clinical Diagnostics written by Thomas O. Joos and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 283 pages. Available in PDF, EPUB and Kindle. Book excerpt: Leading academic and industrial investigators surveys the world of microarray technology, describing in step-by-step detail diverse DNA and protein assays in clinical laboratories using state-of-the-art technologies. The advanced tools and methods described are designed for mRNA expression analysis, SNP analysis, identification, and quantification of proteins, and for studies of protein-protein interactions. The protocols follow the successful Methods in Molecular BiologyTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Download or read book Rapid Test written by Laura Anfossi and published by BoD – Books on Demand. This book was released on 2018-09-26 with total page 104 pages. Available in PDF, EPUB and Kindle. Book excerpt: Rapid tests, also known as point-of-care tests, have been in use for decades in the clinical and medical area and have become increasingly popular as an efficient screening method for conducting on-site analysis thanks to their simplicity, speed, specificity and sensitivity. Nowadays, rapid tests are widely applied for clinical, drug, food, forensic and environmental analysis and fields of application are rapidly increasing together with advances in the technology. The growing interest in rapid tests and their expanding application in diverse fields, together with requirements of improved sensitivity, reliability, multiple detection capacity and robustness, are prompting innovation in the design of novel platforms, and in the exploitation of innovative detection strategies. The book covers advances in materials, technology and test design.
Download or read book Diagnostic Application of Oligonucleotides Microarrays Multiplex Detection of Pathogens by ElectroHydrodynamic Jet Printing written by Jefri Bin Mohd Izham Mahendran and published by . This book was released on 2014 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Protein and Peptide based Microarrays for Multiplex Detection written by Navid Rabiee and published by . This book was released on 2020 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: "This book discusses the aspects of protein and peptide-based microarrays for multiplex detection, based on Rabiee's Theory. It provides a thorough introduction to the field, before examining protein and peptide-based microarrays in greater detail, along with their related problems and solutions. Also covered are the materials and fabrication methods involved with protein microarrays. It offers an outlook on the future of the field within its conclusion and provides an understanding of cutting-edge aspects and horizons of this topic and is an excellent guide for scientists, students and doctors." -- Prové de l'editor.
Download or read book Applying Genomic and Proteomic Microarray Technology in Drug Discovery Second Edition written by Robert S. Matson and published by CRC Press. This book was released on 2013-03-13 with total page 325 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microarrays play an increasingly significant role in drug discovery. The commercial landscape has changed dramatically over the past few years and researchers have made great advancements with regard to construction and use. Now in its second edition, Applying Genomic and Proteomic Microarray Technology in Drug Discovery highlights, describes, and evaluates current scientific research using microarray technology in genomic and proteomic applications. Updated and revised to reflect recent progress in the field, the second edition discusses: Expanded omics-driven applications, including the areas of metabolomics and chemical biology The commercialization of the microarray platform, with a historical perspective aimed at recognizing key technological developments Solid-supports (substrates) and surface chemistries currently used in the creation of nucleic acid and protein microarrays Different approaches to producing microarrays that achieve spot equality with the same number of molecules properly oriented The development of the gene expression microarray and representative applications The development of protein microarray technology, including its history and key applications Unique to this edition is a new chapter on multiplex assays that examines the development and applications of arrays across diverse platforms. It discusses applications for qPCR, multiplex lateral flow, and multiplex bead assays. It also presents platform-to-platform comparisons. Microarrays remain an invaluable tool for omics-based research not only in drug discovery, but in the life sciences, in clinical research, and for diagnostic applications worldwide. This volume presents the current state of the art on the utility of this technology to solve a host of important biological problems.
Download or read book Development of Low cost and Easy to use Tools for Multiplex Pathogen Detection written by Dieter Maurice Tourlousse and published by . This book was released on 2010 with total page 352 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Microarrays written by Kilian Dill and published by Springer Science & Business Media. This book was released on 2008-12-30 with total page 355 pages. Available in PDF, EPUB and Kindle. Book excerpt: Combinatorial chemistry is used to find materials that form sensor microarrays. This book discusses the fundamentals, and then proceeds to the many applications of microarrays, from measuring gene expression (DNA microarrays) to protein-protein interactions, peptide chemistry, carbodhydrate chemistry, electrochemical detection, and microfluidics.
Download or read book Global Infectious Disease Surveillance and Detection written by Institute of Medicine and published by National Academies Press. This book was released on 2007-11-11 with total page 284 pages. Available in PDF, EPUB and Kindle. Book excerpt: Early detection is essential to the control of emerging, reemerging, and novel infectious diseases, whether naturally occurring or intentionally introduced. Containing the spread of such diseases in a profoundly interconnected world requires active vigilance for signs of an outbreak, rapid recognition of its presence, and diagnosis of its microbial cause, in addition to strategies and resources for an appropriate and efficient response. Although these actions are often viewed in terms of human public health, they also challenge the plant and animal health communities. Surveillance, defined as "the continual scrutiny of all aspects of occurrence and spread of a disease that are pertinent to effective control", involves the "systematic collection, analysis, interpretation, and dissemination of health data." Disease detection and diagnosis is the act of discovering a novel, emerging, or reemerging disease or disease event and identifying its cause. Diagnosis is "the cornerstone of effective disease control and prevention efforts, including surveillance." Disease surveillance and detection relies heavily on the astute individual: the clinician, veterinarian, plant pathologist, farmer, livestock manager, or agricultural extension agent who notices something unusual, atypical, or suspicious and brings this discovery in a timely way to the attention of an appropriate representative of human public health, veterinary medicine, or agriculture. Most developed countries have the ability to detect and diagnose human, animal, and plant diseases. Global Infectious Disease Surveillance and Detection: Assessing the Challenges-Finding Solutions, Workshop Summary is part of a 10 book series and summarizes the recommendations and presentations of the workshop.
Download or read book Molecular Microbiology written by David H. Persing and published by John Wiley & Sons. This book was released on 2020-07-24 with total page 835 pages. Available in PDF, EPUB and Kindle. Book excerpt: Presenting the latest molecular diagnostic techniques in one comprehensive volume The molecular diagnostics landscape has changed dramatically since the last edition of Molecular Microbiology: Diagnostic Principles and Practice in 2011. With the spread of molecular testing and the development of new technologies and their opportunities, laboratory professionals and physicians more than ever need a resource to help them navigate this rapidly evolving field. Editors David Persing and Fred Tenover have brought together a team of experienced researchers and diagnosticians to update this third edition comprehensively, to present the latest developments in molecular diagnostics in the support of clinical care and of basic and clinical research, including next-generation sequencing and whole-genome analysis. These updates are provided in an easy-to-read format and supported by a broad range of practical advice, such as determining the appropriate type and quantity of a specimen, releasing and concentrating the targets, and eliminating inhibitors. Molecular Microbiology: Diagnostic Principles and Practice Presents the latest basic scientific theory underlying molecular diagnostics Offers tested and proven applications of molecular diagnostics for the diagnosis of infectious diseases, including point-of-care testing Illustrates and summarizes key concepts and techniques with detailed figures and tables Discusses emerging technologies, including the use of molecular typing methods for real-time tracking of infectious outbreaks and antibiotic resistance Advises on the latest quality control and quality assurance measures Explores the increasing opportunities and capabilities of information technology Molecular Microbiology: Diagnostic Principles and Practice is a textbook for molecular diagnostics courses that can also be used by anyone involved with diagnostic test selection and interpretation. It is also a useful reference for laboratories and as a continuing education resource for physicians.
Download or read book Novel Antibody Microarray Technologies for Multiplex Protein Analysis in Complex Samples written by Huiyan Li and published by . This book was released on 2015 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: "The enzyme-linked immunosorbent assay (ELISA) is the golden standard for the measurement of proteins, but can only detect one protein at each time in the conventional format. Antibody microarrays have been developed to accommodate thousands of micro-spots on a single slide, thus allowing multiple immunoassays to be conducted in parallel. To date antibody microarrays have been used for various applications including the discovery of protein biomarkers for a variety of cancers and other diseases. However, the relatively long assay time from hours to overnight and low multiplexing capability with a maximum of ~50 proteins for sandwich assays are still the main challenges limiting the wide use of antibody microarrays to perform immunoassays. In this dissertation, we have developed novel formats of antibody microarrays to overcome the mass transport and multiplexing limitations, and have demonstrated their use for multiple protein analysis in blood. Specifically, (i) we have developed a beads-in-gel droplet microarray (BiGDM) that encapsulates antibody coated microbeads in 3D hydrogel droplets spotted onto a glass slide. The 3D architecture and the highly porous gels help enhance mass transport of proteins during the assays; (ii) we have established a novel technology named "snap chip" to colocalize cognate capture and detection antibodies in an antibody colocalization microarray (ACM) format by transferring antibodies from microarray-to-microarray in a snap apparatus, thus improving multiplexing capability and simplifying ACM procedure; (iii) we have further improved the multiplexing capability of the snap chip by a double transfer process with improved alignment accuracy, and designed a second version of the portable and convenient-to-use snap apparatus; (iv) we have finally used the snap chip for a serial analysis of multiple proteins in sera during the progression of human breast tumor in a mouse model. The novel antibody microarrays reported in this dissertation have introduced new avenues to overcome mass transport and multiplexing challenges in conventional microarrays, and their use in the detection of multiple proteins in blood indicates that they are promising technologies for the discovery and validation of protein biomarkers for a variety of diseases including notably cancer. " --
Download or read book Antibody Based Strategies for Multiplexed Diagnostics written by Krupa Arun Navalkar and published by . This book was released on 2014 with total page 339 pages. Available in PDF, EPUB and Kindle. Book excerpt: Peptide microarrays are to proteomics as sequencing is to genomics. As microarrays become more content-rich, higher resolution proteomic studies will parallel deep sequencing of nucleic acids. Antigen-antibody interactions can be studied at a much higher resolution using microarrays than was possible only a decade ago. My dissertation focuses on testing the feasibility of using either the Immunosignature platform, based on non-natural peptide sequences, or a pathogen peptide microarray, which uses bioinformatically-selected peptides from pathogens for creating sensitive diagnostics. Both diagnostic applications use relatively little serum from infected individuals, but each approaches diagnosis of disease differently. The first project compares pathogen epitope peptide (life-space) and non-natural (random-space) peptide microarrays while using them for the early detection of Coccidioidomycosis (Valley Fever). The second project uses NIAID category A, B and C priority pathogen epitope peptides in a multiplexed microarray platform to assess the feasibility of using epitope peptides to simultaneously diagnose multiple exposures using a single assay. Cross-reactivity is a consistent feature of several antigen-antibody based immunodiagnostics. This work utilizes microarray optimization and bioinformatic approaches to distill the underlying disease specific antibody signature pattern. Circumventing inherent cross-reactivity observed in antibody binding to peptides was crucial to achieve the goal of this work to accurately distinguishing multiple exposures simultaneously.
Download or read book Microarray Technology for Multiplex Detection of Genetically Modified Organisms GMOS in Food written by Roberta Bordoni and published by . This book was released on 2006 with total page 74 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Protein Microarray for Disease Analysis written by Catherine J. Wu and published by Humana Press. This book was released on 2016-08-23 with total page 373 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protein microarrays have been used for a wide variety of important tasks, such as identifying protein-protein interactions, discovering disease biomarkers, identifying DNA-binding specificity by protein variants, and for characterization of the humoral immune response. In Protein Microarray for Disease Analysis: Methods and Protocols, expert researchers provide concise descriptions of the methodologies currently used to fabricate microarrays for the comprehensive analysis of proteins or responses to proteins that can be used to dissect human disease. These methodologies are the toolbox for revolutionizing drug development and cell-level biochemical understanding of human disease processes. Beginning with a section on protein-detecting analytical microarrays, the volume continues with sections covering antigen microarrays for immunoprofiling, protein function microarrays, the validation of candidate targets, proteomic libraries, as well as signal detection strategies and data analysis techniques. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Practical and cutting-edge, Protein Microarray for Disease Analysis: Methods and Protocols serves as a solid framework to aid scientists in understanding how protein microarray technology is presently developing and how it can be applied to transform our analysis of human disease.
