Download or read book Multiparameter Flow Cytometry in the Diagnosis of Hematologic Malignancies written by Anna Porwit and published by Cambridge University Press. This book was released on 2018-01-25 with total page 248 pages. Available in PDF, EPUB and Kindle. Book excerpt: Master implementation of the techniques of flow cytometry in diagnosing complex haematological diseases and malignancies in patients, worldwide. Featuring World Health Organization recommendations on pre-analytical steps, instrument settings and panel construction, this invaluable manual offers invaluable support for those researching, practising and analyzing the cause of hematological malignancies. Authored by leading experts, this book puts flow-cytometry into everyday context. With a focus on multicolour panels, the manual provides readers an experienced understanding of effective, implementation techniques. Practitioners of all levels are offered a background in a variety of diseases presented alongside the most current methodology. Wide-ranging and comprehensive; detailed images of healthy blood, bone marrow and lymph-nodes are illustrated throughout, allowing for effective diagnosis. Through engaging with differential diagnoses, the manual offers an understanding of similar symptoms and mimicking malignancies, avoiding inaccurate results. Featuring in-depth descriptions of chronic diseases; users can reach accurate diagnosis, first time.
Download or read book Multiparameter Flow Cytometry in the Diagnosis of Hematologic Malignancies written by Anja Porwit and published by Cambridge University Press. This book was released on 2018-01-25 with total page 247 pages. Available in PDF, EPUB and Kindle. Book excerpt: This practical manual offers an active understanding of how to implement flow-cytometry when facing complex, haematological diseases.
Download or read book Flow Cytometry of Hematological Malignancies written by Claudio Ortolani and published by John Wiley & Sons. This book was released on 2011-06-09 with total page 330 pages. Available in PDF, EPUB and Kindle. Book excerpt: Flow Cytometry of Hematological Malignancies contains an array of graphical outputs produced by the technique in the study of the most (and the least) common diseases. The images included allow you to compare your own results with a third party reference pattern. There is a detailed description of the main leukocyte antigens, together with a description of their distribution amongst normal and abnormal blood cells. The book also provides a comprehensive description of the phenotype of every neoplastic blood disease recorded in the WHO classification system, including all the instructions needed to recognise and classify even the least common entity. Designed to be practical, the book is perfect for quick consultation and is divided into two main sections. Section I deals with the direct object of immunophenotyping, and Section II deals with the ultimate target of the analysis. More than 50 antigens are covered and every antigen is dealt with in three main parts: general features, cytometric features and practical hints. This authoritative and state-of-the-art reference will be invaluable for clinicians directly involved in the diagnosis and analysis of hematological diseases, including hematologists, hematopathologists, oncologists, pathologists and technicians working in diagnostic laboratories.
Download or read book Practical Flow Cytometry in Haematology written by Mike Leach and published by John Wiley & Sons. This book was released on 2015-06-18 with total page 416 pages. Available in PDF, EPUB and Kindle. Book excerpt: The analysis of blood, bone marrow and tissue fluid specimens requires a multi-faceted approach with the integration of scientific data from a number of disciplines. No single discipline can operate in isolation or errors will occur. Flow cytometry is in a privileged position in that it can provide rapid analysis of specimens and it is often the first definitive investigation to produce results and help formulate a working diagnosis. This companion text to Practical Flow Cytometry in Haematology Diagnosis contains 100 worked examples drawn from real clinical cases presenting to the authors’ institution. Cases are illustrated with peripheral blood and bone marrow cytology, tissue pathology and cytogenetic and molecular data, which are integrated to generate, where appropriate, a diagnosis based on the WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues. The spectrum of clinical cases includes adult and paediatric patients, and both neoplastic and reactive disorders. The cases appear in no particular order to challenge the reader to make their own diagnosis. The reader will review May−Grünwald−Giemsa (MGG)-stained films of peripheral blood and bone marrow aspirates presented alongside flow cytometric data and haematoxylin and eosin (H&E)-stained bone marrow and other tissue biopsy sections. Immunohistochemistry is used to further clarify the tissue lineage and cell differentiation. Cytogenetic studies using metaphase preparations are used to identify translocations and chromosome gains and losses whilst interphase fluorescence in situ hybridisation (FISH) studies and polymerase chain reaction (PCR) are used to identify gene fusions, gene rearrangements and deletions. Each case concludes with a discussion of the features that are important to making a diagnosis. The cases are also listed according to disease classification in the appendix so that the text can also be used as a reference. Practical Flow Cytometry in Haematology: 100 Worked Examples: Provides a practical, example-based resource for flow cytometry Demonstrates how flow cytometry results should be interpreted and applied to optimize patient care Includes both malignant and benign conditions Can be used in conjunction with Practical Flow Cytometry in Haematology Diagnosis, by the same author team (ISBN 9780470671207) Practical Flow Cytometry in Haematology: 100 Worked Examples is ideal for practicing haematologists and histopathologists with an interest in haematopathology, but particularly directed at trainee haematologists and scientists preparing for FRCPath and related examinations.
Download or read book Flow Cytometry Immunohistochemistry and Molecular Genetics for Hematologic Neoplasms written by Tsieh Sun and published by Lippincott Williams & Wilkins. This book was released on 2011-11-17 with total page 449 pages. Available in PDF, EPUB and Kindle. Book excerpt: Rev. ed. of: Flow cytometry and immunohistochemistry for hematologic neoplasms. c2008.
Download or read book Flow Cytometry and Immunohistochemistry for Hematologic Neoplasms written by Tsieh Sun and published by Lippincott Williams & Wilkins. This book was released on 2008 with total page 362 pages. Available in PDF, EPUB and Kindle. Book excerpt: This text is a detailed guide to the use of flow cytometry, immunohistochemistry, and molecular genetic techniques for diagnosis of hematologic neoplasms. Dr. Sun explains the principles of these techniques and demonstrates their utility in 39 clinical cases covering all important entities. Each case represents a comprehensive diagnostic approach including a clinical history and flow cytometric, immunohistochemical, and molecular genetic findings. Abundant full-color illustrations show histologic sections, immunohistochemical stains, bone marrow, peripheral blood, and body fluid smears, and each case includes a complete set of flow cytometric histograms. Over 100 tables compare and differentiate the diagnostic features of similar diseases. An image bank will be available on a companion Website.
Download or read book An Online Decision Support System for Diagnosing Hematologic Malignancies by Flow Cytometry Immunophenotyping written by You-Wen Qian and published by . This book was released on 2009 with total page 125 pages. Available in PDF, EPUB and Kindle. Book excerpt: Immunophenotyping by flow cytometry (FCM) plays an important role in the diagnosis and subclassification of hematopoietic malignancies. Currently, almost all laboratories manually analyze FCM for interpretation and data entry. This time consuming and labor intensive process calls for a technology based streamlined automation. A decision support system to interpret flow results will be helpful for both hematopathologists and laboratory personnel in any busy laboratories and therefore reduce the healthcare cost. To meet this challenge, there have been several attempts made to potentially automate the diagnostic process of lymphoma and leukemias with only partial resolution of the current drawbacks of manual analysis, let alone the clinical acceptance. In this study, a knowledge-based decision support system to interpret online FCM results for hematologic malignancies has been developed as a complete Client-Server application. The listmode data files are loaded to the system where gating, dot plot, histogram and contour plot can be performed. Upon gating, the CD marker results are generated as a percentage with associated positive or negative designation. The knowledge base for a final diagnosis is based on the current World Health Organization (WHO) classification of hematologic malignancies which is depicted in a semantic network and further embedded in an eXtensible markup language (XML). Differential diagnosis is taken into consideration in our decision support system. The confidence level for a particular differential diagnosis is based on the sensitivity and specificity of a particular CD marker for making a clinical diagnosis, combined with clinical experience as well. Java programming is used to implement the inference engine where tree structure and search algorithm are employed. A set of 273 FCM listmode data files are fed into the system and diagnosis was correctly included in top three differential diagnoses in 94% of all cases tested. In conclusion, the website (http://www.flowcytometryonline.com) has been set up for online FCM analysis and decision support and data transportation. The system is expected to facilitate clinical diagnosis of hematologic malignancies and assist resident teaching as well.
Download or read book Color Atlas text of Flow Cytometric Analysis of Hematologic Neoplasms written by Tsieh Sun and published by Igaku-Shoin Medical Publishers. This book was released on 1993 with total page 240 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Flow Cytometric Analysis of Hematologic Neoplasms written by Tsieh Sun and published by Lww. This book was released on 2002 with total page 276 pages. Available in PDF, EPUB and Kindle. Book excerpt: This thoroughly updated Second Edition provides a comprehensive review of the basic principles, clinical applications, and interpretation of flow cytometry results. Focusing on hematologic neoplasms, it covers current uses and applications while considering cost containment trends in healthcare. More than 30 complete clinical cases with detailed clinical histories, flow cytometric findings, morphologic illustrations, and other pertinent information on molecular biological techniques, cytochemistry, and cytogenetics provide readers with complete clinical information to assist in diagnosis, as well as to hone skills. The text also features 6 new leukemias/lymphomas and 94 new color illustrations, including color photographs of peripheral blood smears, bone marrow smears, bone marrow core biopsies, and histologic sections of lymph nodes, spleen, liver and solid tumors.
Download or read book Flow Cytometry in Evaluation of Hematopoietic Neoplasms written by Sindhu Cherian and published by . This book was released on 2012 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a practical, case-based guide to flow cytometric analysis in the workup of hematopoietic neoplasms presenting in the peripheral blood, marrow, lymphoid tissue, and extranodal sites. Using multi-color techniques pioneered by Brent Wood, the text demonstrates a unique approach to diagnosis of hematopoietic malignancies as well as identification of small abnormal populations in the posttherapy setting (minimal residual disease testing). The publication contains an introduction to immunophenotypic changes seen in normal hematopoiesis along with an overview of the evaluation of lymphomas, leukemias, and myeloid stem-cell neoplasms. These concepts are further illustrated by a series of 36 cases, each dedicated to a specific disease entity. Each case provides detailed, full-color images of flow cytometric dot plots that clearly outline the features of the disease, accompanied by a clinical history and thorough discussion, enabling readers to develop and work through a differential diagnosis and recognize potential diagnostic pitfalls. Take-home points for each case emphasize critical concepts in flow cytometric diagnosis.
Download or read book Flow Cytometry in Hematology written by Ole Didrik Laerum and published by . This book was released on 1992 with total page 304 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book reviews flow cytometric methods (techniques for measuring and sorting of cells) used in hematology--ranging from those in routine use (such as leukocyte counting and immunophentotyping in diseases like leukemia and AIDS) to those that have potential future use in experimental and clinical hematology. This volume will be of interest to a wide audience, including cell biologists, hematologists, cancer researchers, and HIV/AIDS researchers.
Download or read book Flow Cytometry in Neoplastic Hematology written by Wojciech Gorczyca and published by CRC Press. This book was released on 2010-02-25 with total page 350 pages. Available in PDF, EPUB and Kindle. Book excerpt: This highly illustrated, practical guide contains comprehensive coverage of all the important factors for clinical diagnosis with flow cytometry. It explains the general parameters and correlation with color histomorphological findings throughout, taking a systematic approach from basic cases to complicated problem areas. Hematopathologists and neoplastic hematologists will find this book an important resource for keeping up to date with developments in clinical practice. This second edition includes a chapter on antigen expression during myeloid and lymphoid differentiation.
Download or read book Laboratory Hematology Practice written by Kandice Kottke-Marchant and published by John Wiley & Sons. This book was released on 2012-06-06 with total page 777 pages. Available in PDF, EPUB and Kindle. Book excerpt: Expertly edited and endorsed by the International Society for Laboratory Hematology, this is the newest international textbook on all aspects of laboratory hematology. Covering both traditional and cutting-edge hematology laboratory technology this book emphasizes international recommendations for testing practices. Illustrative case studies on how technology can be used in patient diagnosis are included. Laboratory Hematology Practice is an invaluable resource for all those working in the field.
Download or read book Practical Flow Cytometry in Haematology Diagnosis written by Mike Leach and published by John Wiley & Sons. This book was released on 2013-04-22 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The accurate diagnosis of haematologic malignancies is a complex and challenging task. It routinely involves morphologic, molecular, cytogenetic and flow cytometric expertise. To determine what treatment protocol will be followed, it is vital to integrate, interpret and report these results accurately. Flow cytometry is key in this diagnostic pathway. This book guides the reader as to how flow cytometry results should be interpreted and applied to optimize patient care. At the core of this text is an appreciation of clinical, morphological and immunophenotypic correlation and the importance of constant liaison and discussion between the medical and scientific teams. The authors present a logical and practical approach to the diagnosis of blood disorders (both neoplastic and reactive) and evaluate the diagnostic applications of flow cytometry. Practical Flow Cytometry in Haematology Diagnosis provides: A clinical reference source on all aspects of flow cytometry, covering both malignant and benign conditionsCarefully chosen real-life cases in each chapter, complemented by high quality morphological images Help in making a diagnosis, together with an understanding of the limitations of the technique and the potential pitfalls All those who instigate, perform, interpret or act upon flow cytometry patient material will find this book an invaluable guide.
Download or read book Minimal Residual Disease in Hematologic Malignancies written by Raanani P Ed and published by S. Karger AG (Switzerland). This book was released on 2004 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Detection of minimal residual disease (MRD) is increasingly used in the management of leukemia patients. A wide variety of methods have been developed and include technologies designed to detect residual malignant cells beyond the sensitivity of conventional approaches such as morphology and banding cytogenetics in leukemia. The choice of the best method depends on the biology of the individual malignancy, i.e. on the determination of specific markers which are useful to differentiate between leukemic cells and normal hematopoiesis in leukemic patients. These markers include leukocyte differentiation antigens, fusion transcripts, transcripts overexpressed by mutated or nonmutated genes, rearranged genes, and individual markers like polymorphic repetitive DNA sequences. The major technologies for MRD detection, their advantages and disadvantages and their clinical applications are discussed in this special issue - from 'bench to bedside'. Providing a comprehensive overview on the significance of MRD in the evaluation, treatment and follow-up of hematologic malignancies, it will be of great value to hematologists, researchers interested in leukemias and lymphomas as well as laboratory technicians.
Download or read book Pediatric Lymphomas written by Howard J. Weinstein and published by Springer Science & Business Media. This book was released on 2007-01-20 with total page 296 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is a comprehensive textbook of Hodgkin's and non-Hodgkin's lymphomas written by leaders in the field of childhood lymphomas. It includes clinical, pathologic and molecular biology of each subtype of lymphoma. The pathology chapters are comprehensive and include excellent photographs. The book is at the level of subspecialists in pediatric hematology and oncology, radiation oncology, pediatric surgery and hematopathology.
Download or read book Atlas of Lymph Node Pathology written by Amy S. Duffield and published by Lippincott Williams & Wilkins. This book was released on 2020-10-09 with total page 1062 pages. Available in PDF, EPUB and Kindle. Book excerpt: Closely mirroring the daily sign-out process, Atlas of Lymph Node Pathology: A Pattern Based Approach is a highly illustrated, efficient guide to accurate diagnosis. This practical reference uses a proven, pattern-based approach to clearly explain how to interpret challenging cases by highlighting red flags in the clinical chart and locating hidden clues in the slides. Useful as a daily “scope-side guide,” it features numerous clinical and educational features that help you find pertinent information, reach a correct diagnosis, and assemble a thorough and streamlined pathology report.
