Download or read book Monitoring Freshwater Fish Communities with Environmental DNA eDNA Metabarcoding written by Jonas Bylemans and published by . This book was released on 2018 with total page 221 pages. Available in PDF, EPUB and Kindle. Book excerpt: Abstract: "Efficient monitoring and management of species biodiversity requires sensitive and reliable detection tools, especially when management focusses on species that are present at low densities. Recent technological advances now allow biodiversity to be monitored through the DNA that organisms leave behind in the environment (eDNA). These eDNA-based tools are less susceptible to biases and can significantly improve the data from monitoring surveys, which in turn leads to a better management of natural resources. The aim of this thesis is to examine the potential of different DNA fragments for eDNA-based monitoring and evaluate the potential of eDNA metabarcoding as a monitoring tool for freshwater fish communities within the Murray-Darling Basin (MDB, Australia)."
Download or read book Development of an Environmental DNA Method for Monitoring Freshwater Fish Communities Using Metabarcoding written by Jianlong Li and published by . This book was released on 2019 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Environmental DNA written by Pierre Taberlet and published by Oxford University Press. This book was released on 2018-02-02 with total page 272 pages. Available in PDF, EPUB and Kindle. Book excerpt: Environmental DNA (eDNA) refers to DNA that can be extracted from environmental samples (such as soil, water, feces, or air) without the prior isolation of any target organism. The analysis of environmental DNA has the potential of providing high-throughput information on taxa and functional genes in a given environment, and is easily amenable to the study of both aquatic and terrestrial ecosystems. It can provide an understanding of past or present biological communities as well as their trophic relationships, and can thus offer useful insights into ecosystem functioning. There is now a rapidly-growing interest amongst biologists in applying analysis of environmental DNA to their own research. However, good practices and protocols dealing with environmental DNA are currently widely dispersed across numerous papers, with many of them presenting only preliminary results and using a diversity of methods. In this context, the principal objective of this practical handbook is to provide biologists (both students and researchers) with the scientific background necessary to assist with the understanding and implementation of best practices and analyses based on environmental DNA.
Download or read book Multivariate Analysis of Ecological Data using CANOCO 5 written by Petr Šmilauer and published by Cambridge University Press. This book was released on 2014-04-17 with total page 375 pages. Available in PDF, EPUB and Kindle. Book excerpt: An accessible introduction to the theory and practice of multivariate analysis for graduates, researchers and professionals dealing with ecological problems.
Download or read book DNA Barcodes written by Ida Lopez and published by Humana Press. This book was released on 2012-06-12 with total page 470 pages. Available in PDF, EPUB and Kindle. Book excerpt: A DNA barcode in its simplest definition is one or more short gene sequences taken from a standardized portion of the genome that is used to identify species through reference to DNA sequence libraries or databases. In DNA Barcodes: Methods and Protocols expert researchers in the field detail many of the methods which are now commonly used with DNA barcodes. These methods include the latest information on techniques for generating, applying, and analyzing DNA barcodes across the Tree of Life including animals, fungi, protists, algae, and plants. Written in the highly successful Methods in Molecular BiologyTM series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory. Thorough and intuitive, DNA Barcodes: Methods and Protocols aids scientists in continuing to study methods from wet-lab protocols, statistical, and ecological analyses along with guides to future, large-scale collections campaigns.
Download or read book Reservoir Limnology written by Kent W. Thornton and published by John Wiley & Sons. This book was released on 1991-01-16 with total page 262 pages. Available in PDF, EPUB and Kindle. Book excerpt: Addresses reservoirs as unique ecological systems and presents research indicating that reservoirs fall into two or three highly concatenated, interactive ecological systems ranging from riverine to lacustrine or hybrid systems. Includes some controversial concepts about the limnology of reservoirs, which make for interesting reading.
Download or read book Limnology written by Robert G. Wetzel and published by Academic Press. This book was released on 2001-05-10 with total page 1023 pages. Available in PDF, EPUB and Kindle. Book excerpt: Limnology is the study of the structural and functional interrelationships of organisms of inland waters as they are affected by their dynamic physical, chemical, and biotic environments. Limnology: Lake and River Ecosystems, Third Edition, is a new edition of this established classic text. The coverage remains rigorous and uncompromising and has been thoroughly reviewed and updated with evolving recent research results and theoretical understanding. In addition, the author has expanded coverage of lakes to reservoir and river ecosystems in comparative functional analyses.
Download or read book Validation of EDNA Metabarcoding written by Veronica Marian Lee and published by . This book was released on 2022 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Environmental DNA (eDNA) sampling provides a method for assessing fish communities that has potential as a supplement to traditional sampling methods due to its ability to save time as well as its non-invasive nature. This is a method in which from just one sample, eDNA from multiple individual species are able to be sequenced in tandem and the resulting reads identified to describe a community. In order to evaluate this technique and its efficacy for monitoring fish community diversity, we collected water samples alongside surveys performed by the Missouri Department of Conservation sampling program in summers 2020-21. DNA were extracted from these samples and amplified via polymerase chain reaction (PCR), using several targeted mitochondrial gene markers, which were then cleaned and sequenced. We investigated the variation in species detection among different gene markers. We also sought to determine environmental factors involved in variation of eDNA results between summer and winter. We compared the species detected by eDNA to traditional survey detection. Using a variety of statistics including NMDS, ANOSIM, UPGMA clustering, and others, we provide support for the implementation of eDNA metabarcoding techniques to supplement traditional sampling as a robust technique able to provide optimal coverage of fish communities in the Ozarks. We found an average detection rate of 2 species identified by eDNA metabarcoding for every 1 identified by traditional methods"--Abstract, page iii.
Download or read book A Primer of Ecology written by Nicholas J. Gotelli and published by . This book was released on 1998 with total page 236 pages. Available in PDF, EPUB and Kindle. Book excerpt: A detailed exposition of the most common mathematical models in population and community ecology, covering exponential and logistic population growth, age-structured demography, metapopulation dynamics, competition, predation, and island biogeography. Intended to demystify ecological models and the math behind them by deriving the models from first principles. The primer may be used as a self-teaching tutorial, as a primary textbook, or as a supplemental text to a general ecology textbook. Annotation copyright by Book News, Inc., Portland, OR
Download or read book Freshwater Fishes in Britain written by Cynthia E. Davies and published by Brill. This book was released on 2004 with total page 192 pages. Available in PDF, EPUB and Kindle. Book excerpt: Dealing with the detailed occurrence of freshwater fishes in Britain this book is aimed at the general naturalist, as well as anglers, research scientists and those concerned with managing rivers and other waterbodies.
Download or read book Metacommunities written by Marcel Holyoak and published by University of Chicago Press. This book was released on 2005-10 with total page 527 pages. Available in PDF, EPUB and Kindle. Book excerpt: Takes the hallmarks of metapopulation theory to the next level by considering a group of communities, each of which may contain numerous populations, connected by species interactions within communities and the movement of individuals between communities. This book seeks to understand how communities work in fragmented landscapes.
Download or read book Freshwater Biodiversity written by David Dudgeon and published by Cambridge University Press. This book was released on 2020-05-21 with total page 517 pages. Available in PDF, EPUB and Kindle. Book excerpt: Growing human populations and higher demands for water impose increasing impacts and stresses upon freshwater biodiversity. Their combined effects have made these animals more endangered than their terrestrial and marine counterparts. Overuse and contamination of water, overexploitation and overfishing, introduction of alien species, and alteration of natural flow regimes have led to a 'great thinning' and declines in abundance of freshwater animals, a 'great shrinking' in body size with reductions in large species, and a 'great mixing' whereby the spread of introduced species has tended to homogenize previously dissimilar communities in different parts of the world. Climate change and warming temperatures will alter global water availability, and exacerbate the other threat factors. What conservation action is needed to halt or reverse these trends, and preserve freshwater biodiversity in a rapidly changing world? This book offers the tools and approaches that can be deployed to help conserve freshwater biodiversity.
Download or read book Intermittent Rivers and Ephemeral Streams written by Thibault Datry and published by Academic Press. This book was released on 2017-07-11 with total page 624 pages. Available in PDF, EPUB and Kindle. Book excerpt: Intermittent Rivers and Ephemeral Streams: Ecology and Management takes an internationally broad approach, seeking to compare and contrast findings across multiple continents, climates, flow regimes, and land uses to provide a complete and integrated perspective on the ecology of these ecosystems. Coupled with this, users will find a discussion of management approaches applicable in different regions that are illustrated with relevant case studies. In a readable and technically accurate style, the book utilizes logically framed chapters authored by experts in the field, allowing managers and policymakers to readily grasp ecological concepts and their application to specific situations. - Provides up-to-date reviews of research findings and management strategies using international examples - Explores themes and parallels across diverse sub-disciplines in ecology and water resource management utilizing a multidisciplinary and integrative approach - Reveals the relevance of this scientific understanding to managers and policymakers
Download or read book Molecular Techniques for the Identification of Freshwater Fish Species for Environmental Monitoring Programs written by Emily Hulley and published by . This book was released on 2018 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Reliable species identification methods are important for industrial environmental monitoring programs. Probe based real-time PCR (qPCR) provides an accurate, cost-effective and high-throughput method for species identification. Here we present the development and validation of species-specific primers and probe for the identification of eight freshwater fish species. The development of a fully automated species-decoder algorithm allowed for target species identification with 100% accuracy while completely removing any false-positive detection of non-target species. Furthermore, the probe-based qPCR technique utilized in this study is substantially more cost-effective and time efficient than DNA barcoding and morphological identification methods. The qPCR assays were also highly sensitive and accurately detected target species from collected environmental DNA (eDNA) samples. In summary, probe-based multiplex qPCR assays provide a rapid and accurate method for freshwater fish species identification and the methodology established in this study can be utilized for various other species identification initiatives.
Download or read book River Networks as Ecological Corridors written by Andrea Rinaldo and published by Cambridge University Press. This book was released on 2020-10-22 with total page 457 pages. Available in PDF, EPUB and Kindle. Book excerpt: A summary of state-of-the-art research on how the river environment impacts biodiversity, species invasions, population dynamics, and the spread of waterborne disease. Blending laboratory, field and theoretical studies, it is the go-to reference for graduate students and researchers in river ecology, hydrology, and epidemiology.
Download or read book The Biology and Identification of the Coccidia Apicomplexa of Carnivores of the World written by Donald W. Duszynski and published by Academic Press. This book was released on 2018-06-29 with total page 740 pages. Available in PDF, EPUB and Kindle. Book excerpt: The fundamental concept of The Biology and Identification of the Coccidia (Apicomplexa) of Carnivores of the World is to provide an up-to-date reference guide to the identification, taxonomy, and known biology of apicomplexan intestinal and tissue parasites of carnivores including, but not limited to, geographic distribution, prevalence, sporulation, prepatent and patent periods, site(s) of infection in the definitive and (if known) intermediate hosts, endogenous development, cross-transmission, pathology, phylogeny, and (if known) their treatments. These data will allow easy parasite recognition with a summation of virtually everything now known about the biology of each parasite species covered. The last (very modest) and only treatise published on this subject was in 1981 so this book fills a fundamental gap in our knowledge of what is now known, and what is not, about the coccidian parasites that infect and sometimes kill carnivores and/or their prey that can harbor intermediate stages, including many domestic and game animals. - Offers line drawings and photomicrographs of many parasite species that will allow easy diagnosis and identification by both laypersons and professionals (veterinarians, wildlife biologists, etc.) - Presents a complete historical rendition of all known publications on carnivore coccidia for all carnivore families and evaluates the scientific and scholarly merit of each apicomplexan species relative to the current body of knowledge - Provides a complete species analysis and their known biology of all coccidia described from each carnivore lineage and species - Reviews the most current taxonomy of carnivores and their phylogenetic relationships to help assess host-specificity patterns that may be apparent - Evaluates what little cross-transmission work is available to help understand the complexities of those coccidians that use two hosts (e.g., Sarcocystis, Besnoitia, and others) - Provides known treatments for the various parasite genera/species
Download or read book DNA metabarcoding written by Jerney, Jacqueline and published by Nordic Council of Ministers. This book was released on 2023-04-12 with total page 68 pages. Available in PDF, EPUB and Kindle. Book excerpt: We present guidelines for using environmental DNA metabarcoding, together with conventional techniques, to monitor the taxonomic diversity of phytoplankton in marine and brackish waters. The focus is on eukaryotic and prokaryotic phytoplankton using 18S and 16S rRNA primers and high-throughput sequencing. Information and recommendations on sampling, sample processing, molecular biological work, quality control, bioinformatics, data storage and management and cost estimates are included so that the method can be used to complement standardized light microscopy. A scientific literature review, discussion on future perspectives, reference databases and standardization are included. Using eDNA metabarcoding to complement standardized light microscopy advances conventional monitoring and research on phytoplankton communities to assess biodiversity and the state of the marine environment.
