Download or read book Molecular Tools for the Detection and Quantification of Toxigenic Cyanobacteria written by Rainer Kurmayer and published by John Wiley & Sons. This book was released on 2017-09-05 with total page 446 pages. Available in PDF, EPUB and Kindle. Book excerpt: A guide to state-of-the-art molecular tools for monitoring and managing the toxigenicity of cyanobacteria Runaway eutrophication and climate change has made the monitoring and management of toxigenic organisms in the world’s bodies of water more urgent than ever. In order to influence public policy regarding the detection and quantification of those organisms, it is incumbent upon scientists to raise the awareness of policy makers concerning the increased occurrence of toxigenic cyanobacteria and the threats they pose. As molecular methods can handle many samples in short time and help identify toxigenic organisms, they are reliable, cost-effective tools available for tracking toxigenic cyanobacteria worldwide. This volume arms scientists with the tools they need to track toxigenicity in surface waters and food supplies and, hopefully, to develop new techniques for managing the spread of toxic cyanobacteria. This handbook offers the first comprehensive treatment of molecular tools for monitoring toxigenic cyanobacteria. Growing out of the findings of the landmark European Cooperation in Science and Technology Cyanobacteria project (CYANOCOST), it provides detailed, practical coverage of the full array of available molecular tools and protocols, from water sampling, nucleic acid extraction, and downstream analysis—including PCR and qPCR based methods—to genotyping (DGGE), diagnostic microarrays, and community characterization using next-gen sequencing techniques. Offers an overview of the latest trends in the field, while providing a foundation for understanding and applying the tools and techniques described Provides detailed coverage of the full range of molecular tools currently available, with expert guidance on the analysis and interpretation of results Includes step-by-step guidance on standard operational procedures, including molecular tests used in environmental monitoring, with individual chapters devoted to each procedure Complements the published Handbook of Cyanobacterial Monitoring and Cyanotoxin Analysis from the CyanoCOST project This handbook is an indispensable working resource for scientists, lab technicians, and water management professionals and an excellent text/reference for graduate students and supervisors who use molecular tools. It will also be of great value to environmental health and protection officials and policy makers.

Download or read book Protocols for Cyanobacteria Sampling and Detection of Cyanotoxin written by N. Thajuddin and published by Springer Nature. This book was released on 2024-01-19 with total page 504 pages. Available in PDF, EPUB and Kindle. Book excerpt: This protocol book provides detailed procedures for the isolation of cyanobacteria, extraction, quantification, and detection of cyanotoxins. It illustrates the sampling and processing of toxin-producing cyanobacteria in water and aquatic animal samples, detection of cyanotoxins from Anabaena, Anabaenopsis, Cylindrospermopsis, Microsystis, Microcystis, Nodularia, Nostoc, Schizotrix, Lyngbya, Raphidiopsis, Oscillatoria, Planktothrix in aquatic resources. It also covers toxicity analysis by various bioassay protocols, and in vitro and insilico analysis methods. The book also reviews the methods for cyanotoxin extraction, detection, and quantification by various tools including LC-MS/MS, HPLC, NMR, PCR, and HESI-MS/MS. A separate section is dedicated to the advanced methods in Cyanotoxin analysis including the Molecular Imprinting Method (MIM), Cellular signaling biosensor, Electrochemical sensor, Nanosensors, and screening of Polyketide synthase gene. The analysis of various toxin-producing genes like sxtA and mcy is also accounted for in this book. In a nutshell, the book gives comprehensive procedures about the basics and preliminary processes that are involved in sample collection to advanced methods incorporated into the well-explored and unexplored Cyanobacterial toxin. Consequently, this manual is useful for both beginners and advanced researchers, including postgraduate students, academicians, researchers, and scientists in the field of Cyanobacterial research. ​

Download or read book Molecular Methods for the Identification and Uantification of Cyanobacteria in Surface Water Sources written by Treyton Michael Moore and published by . This book was released on 2019 with total page 55 pages. Available in PDF, EPUB and Kindle. Book excerpt: This study investigates the use of quantitative Polymerase Chain Reaction (qPCR) for the early detection of geosmin-producing cyanobacteria. qPCR can detect geosmin-producing cyanobacteria via their DNA. I developed a qPCR assay for this study that is capable of sensitively detecting multiple strains of the geosmin-producing Nostoc genus. The developed assay showed high sensitivity, demonstrating the possibility for its use in detecting low concentrations of geosmin-producing cyanobacteria before detectible levels of geosmin have been produced and released into the water. Through further sequencing of more geosmin-producing genera and species, the methodology outlined in this research could be applied to develop the tools necessary to predict taste-and-odor events caused by geosmin-producing cyanobacteria.

Download or read book Early Detection of Cyanobacterial Toxins Using Genetic Methods written by J. Paul Rasmussen and published by American Water Works Association. This book was released on 2008 with total page 160 pages. Available in PDF, EPUB and Kindle. Book excerpt: This research sought to develop and test rapid genetic methods to identify toxic cyanobacteria. The key objectives were to (1) conduct a literature review and industry questionnaire examining options for rapid genetic tests; (2) characterize and understand the genes involved in cylindrospermopsin and anatoxin production; (3) adapt conventional PCR assays to real-time PCR; (4) develop rapid field methods for DNA preparation; and (5) develop probes for toxin genes and testing in the field.

Download or read book Detection Methods for Cynobacterial toxins written by G A Codd and published by Elsevier. This book was released on 1994-01-01 with total page 202 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cyanobacteria, or blue-green algae, commonly occur in a variety of water types throughout the world. A variable, but, high proportion of the cyanobacterial blooms and scums, which can develop annually in lakes, reservoirs, canals and slow-flowing rivers, contain potent toxins. Although animal poisonings and human health problems associated with the ingestion of, or contact with, cyanobacterial scums have long been recognized, a developing understanding of the health hazards posed by the toxins requires that reliable, sensitive, specific and convenient methods are available for their detection and quantification.Detection methods for cynobacterial toxins looks at the application of biological, toxicological, biochemical and physicochemical techniques in studies of cyanobacterial toxins and at experimental methods that have identified at least 50 cyanobacterial compounds toxic to vertebrates.Academics, public health bodies, environmental protection agencies and water companies are all involved in monitoring cyanobacterial levels and effects, and this book will help to ensure that their research is not duplicated, is standardized and comparable, and that optimal progress can be made in an important area that is still in its developing stages.

Download or read book Toxic Cyanobacteria in Water written by Ingrid Chorus and published by CRC Press. This book was released on 2021-03-08 with total page 701 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cyanobacterial toxins are among the hazardous substances most widely found in water. They occur naturally, but concentrations hazardous to human health are usually due to human activity. Therefore, to protect human health, managing lakes, reservoirs and rivers to prevent cyanobacterial blooms is critical. This second edition of Toxic Cyanobacteria in Water presents the current state of knowledge on the occurrence of cyanobacteria and cyanotoxins as well as their impacts on health through water-related exposure pathways, chiefly drinking-water and recreational activity. It provides scientific and technical background information to support hazard identification, assessment and prioritisation of the risks posed by cyanotoxins, and it outlines approaches for their management at each step of the water-use system. It sets out key practical considerations for developing management strategies, implementing efficient measures and designing monitoring programmes. This enables stakeholders to evaluate whether there is a health risk from toxic cyanobacteria and to mitigate it with appropriate measures. This book is intended for those working on toxic cyanobacteria with a specific focus on public health protection. It intends to empower professionals from different disciplines to communicate and cooperate for sustainable management of toxic cyanobacteria, including public health workers, ecologists, academics, and catchment and waterbody managers. Ingrid Chorus headed the department for Drinking-Water and Swimming-Pool Hygiene at the German Environment Agency. Martin Welker is a limnologist and microbiologist, currently with bioMérieux in Lyon, France.

Download or read book Handbook of Cyanobacterial Monitoring and Cyanotoxin Analysis written by Jussi Meriluoto and published by John Wiley & Sons. This book was released on 2017-01-30 with total page 576 pages. Available in PDF, EPUB and Kindle. Book excerpt: A valuable handbook containing reviews, practical methods and standard operating procedures. A valuable and practical working handbook containing introductory and specialist content that tackles a major and growing field of environmental, microbiological and ecotoxicological monitoring and analysis Includes introductory reviews, practical analytical chapters and a comprehensive listing of almost thirty Standard Operating Procedures (SOPs) For use in the laboratory, in academic and government institutions and industrial settings Those readers will appreciate the research that validates and updates cyanotoxin monitoring and analysis plus adding to approaches for setting standard methods that can be applied worldwide. Wayne Carmichael, Analytical and Bioanalytical Chemistry (2018).

Download or read book Cyanobacteria written by Wael N. Hozzein and published by BoD – Books on Demand. This book was released on 2022-01-12 with total page 106 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cyanobacteria are oxygenic organisms that play crucial roles in the cycles of carbon, nitrogen, and oxygen. They are ideal model organisms for studying photosynthesis, nitrogen fixation, and other biological processes. In addition, cyanobacteria are well recognized for their potential for a variety of biotechnological applications. This book presents a comprehensive overview of this interesting and useful group of bacteria. Chapters discuss such topics as the molecular methods applied for identifying freshwater toxigenic cyanobacteria, the diverse industrial applications of cyanobacteria, the potential of cyanobacteria in wound healing, the production of a novel hemoglobin by Synechocystis sp. PCC 6803, and the diversity, distribution, and applications of cyanobacteria in the Brazilian coastline.

Download or read book Expanding Horizon of Cyanobacterial Biology written by Prashant Kumar Singh and published by Academic Press. This book was released on 2022-06-26 with total page 300 pages. Available in PDF, EPUB and Kindle. Book excerpt: Expanding Horizon of Cyanobacterial Biology discusses the different aspects of cyanobacteria cyanobacterial application, providing a better understanding of cyanobacterial metabolism. Chapters deal with cyanobacteria applications and explore how to exploit cyanobacterial metabolism for industrial applications. Sections cover cyanobacterial applications for the production of nanoparticles, cyanobacterial diversity, and the characterization of different assemblages such as cyanolichens and cyanobacterial endophytes, along with their ecological, morphological and physiological aspects. In addition, bioactive compounds and their applications are explored. Increasing attention has been paid by scientists across the globe to Cyanobacteria as they are ubiquitous microbes and, undoubtedly, an important organism in terms of carbon as well as nitrogen fixation. However, the research on these organisms is limited in terms of their diversity and distribution across the globe. - Provides background knowledge for researchers concerned with cyanobacterial diversity and characterization of different assemblages - Describes the exploitation possibility of cyanobacterial species for human welfare - Discusses the different aspects of cyanobacteria, cyanobacterial application and better understanding of cyanobacterial metabolism - Deals with the exploitation of cyanobacteria and their mats for bioremediation purposes - Includes cyanobacterial nanotechnology and its applications in industry and allied sectors

Download or read book Methods in Cyanobacterial Research written by Shailendra Pratap Singh and published by CRC Press. This book was released on 2024-06-14 with total page 252 pages. Available in PDF, EPUB and Kindle. Book excerpt: This laboratory reference compiles the essential protocols used in cyanobacterial research. Cyanobacteria is a model system for biofuel production and sequestration of carbon dioxide to tackle global climate change due to increasing levels of greenhouse gases. Topics range from identification and growth parameters to biomolecule estimation and isolation. It also covers genome analysis and bioengineering of cyanobacteria for novel products. Key Features: Provides step-by-step procedures and troubleshooting tips Covers identification, growth parameters and pigment estimation methodologies Describes isolation, characterization and purification of microcystin, phycobiliproteins and scytonemin from cyanobacteria Discusses genomics, proteomics and bioengineering of cyanobacteria from novel products Explains sample preparation and visualization protocol for electron microscopy-based analysis of cyanobacteria This collection is useful to students and researchers in life sciences. It is also meant for industry experts who are involved in the production of biofuels, biofertilizers, other value-added products, and carbon sequestration using cyanobacteria.

Download or read book Handbook of Algal Science Technology and Medicine written by Ozcan Konur and published by Academic Press. This book was released on 2020-03-28 with total page 738 pages. Available in PDF, EPUB and Kindle. Book excerpt: Handbook of Algal Science, Microbiology, Technology and Medicine provides a concise introduction to the science, biology, technology and medical use of algae that is structured on the major research fronts of the last four decades, such as algal structures and properties, algal biomedicine, algal genomics, algal toxicology, and algal bioremediation, algal photosystems, algal ecology, algal bioenergy and biofuels. It also covers algal production for biomedicine, algal biomaterials, and algal medicinal foods within these primary sections. All chapters are authored by the leading researchers in their respective research fields. Our society currently faces insurmountable challenges in the areas of biomedicine and energy in the face of increasing global population and diminishing natural resources as well as the growing environmental and economic concerns, such as global warming, greenhouse gas emissions and climate change. Algae offer a way to deal with these challenges and concerns for both sustainable and environment friendly bioenergy production and in biomedicine through the development of crucial biotechnology. Provides an essential interdisciplinary introduction and handbook for all the stakeholders engaged in science, technology and medicine of algae Covers the major research streams of the last four decades, ranging from algal structures, to algal biomedicine and algal bioremediation Fills a significant market opening for an interdisciplinary handbook on algal science, technology and medicine

Download or read book Handbook of Cyanobacterial Monitoring and Cyanotoxin Analysis written by Jussi Meriluoto and published by John Wiley & Sons. This book was released on 2016-12-08 with total page 886 pages. Available in PDF, EPUB and Kindle. Book excerpt: A valuable handbook containing reviews, practical methods and standard operating procedures. A valuable and practical working handbook containing introductory and specialist content that tackles a major and growing field of environmental, microbiological and ecotoxicological monitoring and analysis Includes introductory reviews, practical analytical chapters and a comprehensive listing of almost thirty Standard Operating Procedures (SOPs) For use in the laboratory, in academic and government institutions and industrial settings Those readers will appreciate the research that validates and updates cyanotoxin monitoring and analysis plus adding to approaches for setting standard methods that can be applied worldwide. Wayne Carmichael, Analytical and Bioanalytical Chemistry (2018).

Download or read book Advancing Knowledge on Cyanobacterial Blooms in Freshwaters written by Elisabeth (Savi) Vardaka and published by MDPI. This book was released on 2020-11-13 with total page 202 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cyanobacterial blooms are a water quality problem that is widely acknowledged to have detrimental ecological and economic effects in drinking and recreational water supplies and fisheries. There is increasing evidence that cyanobacterial blooms have increased globally and are likely to expand in water resources as a result of climate change. Of most concern are cyanotoxins, along with the mechanisms that induce their release and determine their fate in the aquatic environment. These secondary metabolites pose a potential hazard to human health and agricultural and aquaculture products that are intended for animal and human consumption; therefore, strict and reliable control of cyanotoxins is crucial for assessing risk. In this direction, a deeper understanding of the mechanisms that determine cyanobacterial bloom structure and toxin production has become the target of management practices. This Special Issue, entitled “Advancing Knowledge on Cyanobacterial Blooms in Freshwaters”, aims to bring together recent multi- and interdisciplinary research, from the field to the laboratory and back again, driven by working hypotheses based on any aspect of mitigating cyanobacterial blooms, from ecological theory to applied research.

Download or read book Detection and Quantification of Genes Responsible for Toxin Biosynthesis in Cyanobacteria from Drinking Water Supplies written by Wenyu Zhang and published by . This book was released on 2008 with total page 14 pages. Available in PDF, EPUB and Kindle. Book excerpt: Late August 2008 a blue green algal bloom broke out at Warragamba Dam, Sydney and cyanobacterial toxins were detected in Wingecaribee water sources. Raw water samples have been continuously analyzed for presence of hepatotoxic peptide microcystin. The aim of this project is to quantify the amount of the 16S rRNA genes and mcyB genes from water samples using quantitative real-time PCR (qRT-PCR).

Download or read book Cyanobacteria Biotechnology written by Paul Hudson and published by John Wiley & Sons. This book was released on 2021-04-20 with total page 560 pages. Available in PDF, EPUB and Kindle. Book excerpt: Unites a biological and a biotechnological perspective on cyanobacteria, and includes the industrial aspects and applications of cyanobacteria Cyanobacteria Biotechnology offers a guide to the interesting and useful features of cyanobacteria metabolism that keeps true to a biotechnology vision. In one volume the book brings together both biology and biotechnology to illuminate the core acpects and principles of cyanobacteria metabolism. Designed to offer a practical approach to the metabolic engineering of cyanobacteria, the book contains relevant examples of how this metabolic "module" is currently being engineered and how it could be engineered in the future. The author includes information on the requirements and real-world experiences of the industrial applications of cyanobacteria. This important book: Brings together biology and biotechnology in order to gain insight into the industrial relevant topic of cyanobacteria Introduces the key aspects of the metabolism of cyanobacteria Presents a grounded, practical approach to the metabolic engineering of cyanobacteria Offers an analysis of the requirements and experiences for industrial cyanobacteria Provides a framework for readers to design their own processes Written for biotechnologists, microbiologists, biologists, biochemists, Cyanobacteria Biotechnology provides a systematic and clear volume that brings together the biological and biotechnological perspective on cyanobacteria.

Download or read book Design of Customized Molecular Tools to Strengthen the Metabolic Engineering of Cyanobacteria written by Franz Opel and published by . This book was released on 2023 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Advancing Knowledge on Cyanobacterial Blooms in Freshwaters written by Konstantinos Ar Kormas and published by . This book was released on 2020 with total page 202 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cyanobacterial blooms are a water quality problem that is widely acknowledged to have detrimental ecological and economic effects in drinking and recreational water supplies and fisheries. There is increasing evidence that cyanobacterial blooms have increased globally and are likely to expand in water resources as a result of climate change. Of most concern are cyanotoxins, along with the mechanisms that induce their release and determine their fate in the aquatic environment. These secondary metabolites pose a potential hazard to human health and agricultural and aquaculture products that are intended for animal and human consumption; therefore, strict and reliable control of cyanotoxins is crucial for assessing risk. In this direction, a deeper understanding of the mechanisms that determine cyanobacterial bloom structure and toxin production has become the target of management practices. This Special Issue, entitled “Advancing Knowledge on Cyanobacterial Blooms in Freshwaters”, aims to bring together recent multi- and interdisciplinary research, from the field to the laboratory and back again, driven by working hypotheses based on any aspect of mitigating cyanobacterial blooms, from ecological theory to applied research.