Download or read book Molecular Thermodynamics of Protein Interactions and Phase Behavior in Aqueous Electrolyte Solution written by Christopher James Coen and published by . This book was released on 1995 with total page 460 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Molecular Thermodynamics of Protein Phase Behavior in Aqueous Electrolyte Solutions written by John Joseph Grigsby and published by . This book was released on 2001 with total page 290 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Molecular Thermodynamics of Protein Interactions and Phase Equilibria in Aqueous Electrolyte Solutions written by Daniel Edward Kuehner and published by . This book was released on 1998 with total page 454 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Molecular Thermodynamics of Proteins in Aqueous Solutions of Concentrated Electrolyte written by Robin Andrew Curtis and published by . This book was released on 2000 with total page 568 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Modeling of Thermodynamic Properties in Biological Solutions written by Luca F. Cameretti and published by Cuvillier Verlag. This book was released on 2009 with total page 151 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Role of Protein protein Interactions in the Phase Behavior of Aqueous Protein Solutions written by Camille O. Anderson and published by . This book was released on 2001 with total page 454 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Thermodynamic Modeling of Protein Interactions and Phase Behavior written by Leigh Quang and published by . This book was released on 2012 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Protein phase behavior encompasses the formation of dense phases, which include amorphous aggregates, gels, dense liquids, and crystals. The major solution variables that dictate the type of dense phase that is formed are pH, temperature, type of precipitant, precipitant concentration, and protein concentration. Because of the large parameter space and rich variety of phase transitions possible, protein phase behavior is a complex phenomenon. Fundamentally, macroscopic phase transitions are governed by the molecular interactions between proteins in solution. One promising way of quantifying protein-protein interactions and relating them to phase behavior is through the osmotic second virial coefficient B22, a dilute-solution property that characterizes two-particle interactions. The relationship of B22 to overall phase behavior of proteins is explored in this work. The goal of this thesis is to quantitatively relate protein-protein interactions to protein phase diagrams in order to develop predictive models of phase behavior under different solution conditions. A continuum-level approach is used initially to relate experimental B22 data and phase diagrams of proteins by appealing to existing thermodynamic models, with the expectation that a simple continuum model could provide a useful mechanistic framework for predicting protein phase behavior. The first approach attempted was to relate protein interactions and phase behavior within the Flory-Huggins theory of polymer solutions. The second approach utilized the model of Haas and Drenth, which is based on the free energy of mixing for hard spheres. Finally, phase equilibrium was predicted from virial coefficients using the osmotic virial equation. A qualitative relationship was found between B22 and phase behavior from these continuum models; however, quantitative agreement could not be obtained. The isotropic assumption shared among these models in addition to the orientationally-averaged nature of B22 suggests that the anisotropic character of protein interactions cannot be neglected, demonstrating the need for more detailed molecular-level models. The role of anisotropy in protein interactions was explored through analysis of "patch-antipatch" pairs in the computation of B22 in atomistic detail. Patch-antipatch pairs represent highly attractive orientations resulting from geometric complementarity between protein surfaces. Previous work used simple Monte Carlo integration for the calculation of B22 from atomistic models of proteins. However, the presence of patch-antipatch pairs led to significant numerical concerns. These concerns warranted a reexamination of the numerical methods for computing B22. A hybrid Monte Carlo/patch integration approach is utilized to calculate B22 for lysozyme and chymosin B. This method involves a combination of numerical integration techniques in an attempt to obtain better convergence in predicting B22. The overall B22 for the proteins studied was separated into three components: contributions from the excluded volume, from the patch-antipatch pairs, and from background configurations. The excluded volume component was found to be adequately determined using simple Monte Carlo integration. The contributions from individual patch-antipatch pairs were accounted for by carefully integrating the subregions of the configuration space occupied by these pairs using a globally adaptive integration routine. The background component to B22 was also calculated by simple Monte Carlo integration in which the regions of the configuration space occupied by the patch-antipatch pairs were excluded. The calculations performed that account for the full protein structure emphasize the importance of several features of protein interactions. First, the difference in the interaction behavior of the two proteins studied was found to be largely attributed to the charge anisotropy of patch-antipatch pairs. However, the relation of the results to experimental data is limited by the omission of accounting for the specific hydration of proteins. Hydration effects are known to affect, and usually attenuate, patch-antipatch configurations, and therefore would be expected to significantly impact the accurate prediction of B22. Classical colloidal as well as atomistic models that omit these important features are inadequate in providing a quantitative representation of protein interactions for a wide range of solution conditions.
Download or read book Molecular Thermodynamics of Electrolyte Solutions written by Lloyd L. Lee and published by World Scientific. This book was released on 2008 with total page 264 pages. Available in PDF, EPUB and Kindle. Book excerpt: The introductory textbook provides an update on electrolyte thermodynamics with a molecular perspective. It is eminently suited as an introduction to the solution thermodynamics of ionic mixtures at the undergraduate and graduate level. It is also invaluable for the understanding and design in the engineering of natural gas treating and adsorption refrigeration with electrolytes.
Download or read book Biothermodynamics written by Urs von Stockar and published by CRC Press. This book was released on 2013-05-30 with total page 628 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book covers the fundamentals of the rapidly growing field of biothermodynamics, showing how thermodynamics can best be applied to applications and processes in biochemical engineering. It describes the rigorous application of thermodynamics in biochemical engineering to rationalize bioprocess development and obviate a substantial fraction of t
Download or read book Protein Interactions and Phase Behavior in Aqueous Solutions written by Andre C. Dumetz and published by ProQuest. This book was released on 2008 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Handbook of Aqueous Electrolyte Thermodynamics written by Joseph F. Zemaitis, Jr. and published by John Wiley & Sons. This book was released on 1986-06-15 with total page 883 pages. Available in PDF, EPUB and Kindle. Book excerpt: Expertise in electrolyte systems has become increasingly important in traditional CPI operations, as well as in oil/gas exploration and production. This book is the source for predicting electrolyte systems behavior, an indispensable "do-it-yourself" guide, with a blueprint for formulating predictive mathematical electrolyte models, recommended tabular values to use in these models, and annotated bibliographies. The final chapter is a general recipe for formulating complete predictive models for electrolytes, along with a series of worked illustrative examples. It can serve as a useful research and application tool for the practicing process engineer, and as a textbook for the chemical engineering student.
Download or read book Protein protein and Protein salt Interactions in Concentrated Aqueous Electrolyte Solutions written by Robin Andrew Curtis and published by . This book was released on 1996 with total page 220 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book A Molecular Approach to Electrolyte Solutions written by Kevin Leslie Gering and published by . This book was released on 1989 with total page 316 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Dissertation Abstracts International written by and published by . This book was released on 2002 with total page 734 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Effects of Cations on Protein Interactions in Aqueous Electrolyte Solutions written by Richard Peter Hom and published by . This book was released on 1999 with total page 274 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Role of Protein Protein Interactions in the Phase Behaviour of Aqueous Protein Solutions written by and published by . This book was released on 2006 with total page 197 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Protein protein Interactions in Aqueous Electrolyte Solutions from Membrane Osmometry written by Camille O. Anderson and published by . This book was released on 1997 with total page 152 pages. Available in PDF, EPUB and Kindle. Book excerpt:
