Download or read book Molecular Studies on the Gene Structure and Expression of Atlantic Salmon Growth Hormone written by James Bradley Lorens and published by . This book was released on 1992 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Family specific Differences in Growth Rate and Hepatic Gene Expression in Triploid Growth Hormone GH Transgenic Atlantic Salmon Salmo Salar L written by Qingheng Xu and published by . This book was released on 2012 with total page 560 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Identification and Annotation of Full length Genes in Atlantic Salmon Salmo Salar written by Jong S. Leong and published by . This book was released on 2010 with total page 248 pages. Available in PDF, EPUB and Kindle. Book excerpt: Large-scale expressed sequence tags (ESTs) in Atlantic salmon (Salmo salar) are examined to answer questions regarding salmonid transcriptomes. ESTs represent raw and incomplete gene sequences that need to be read, assembled and analyzed with computer software. The goal of this thesis was to develop an automatically curated and publicly accessible set of annotated full-length genes, representing a near-complete transcript set for Salmo salar. In turn, these genes provide the framework for studies in gene expression, conservation, and molecular evolution. The work presented here also touches on the results of a molecular evolution study, as an example of how full-length gene identification can be used to answer biological questions. Previous to this study, a limited number of Atlantic salmon cDNA libraries and ESTs were known. To further the goal of determining complete gene sequences, highly enriched full-length cDNA libraries and full-length libraries were created and sequenced, resulting in the ability to identify a large number of full-length reference genes. Together, all libraries represent a diverse pool of transcriptome sequences for Salmo salar. The goal of producing an accurate large-scale full-length gene set on a duplicated genome is not trivial. Complete systems for this objective do not readily exist. EST sequencing, EST assembly, and data storage, are just a few of the initial computational issues that are addressed. Once these issues are resolved, the multi-step workflow of full-length gene determination is described. The final challenge involving the development of a concise and universally accessible system for visualization is discussed. The resulting computational framework that has been developed is shown to be able to handle the intricacies and the size of a duplicated salmonid genome. It has been largely accepted that Atlantic salmon have undergone a recent genome duplication. Gene paralogs provide one source of evidence for this event. Analysis of paralogs revealed signatures of asymmetric evolution possibly due to relaxation of selective pressure. This thesis provides a complete Bioinformatics analysis pipeline to analyze and to visualize a set of full-length reference genes for Atlantic salmon. Using full-length genes as a framework, the topic of molecular evolution was addressed to show evidence of asymmetrical evolution among gene duplicates. The full-length reference genes, along with ESTs and all putative transcripts, have been made publicly available. These results serve as a valuable genomic resource for next-generation sequencing and for all other salmonid research endeavours.
Download or read book Studies on Gene Expression in Atlantic Salmon Salmo Salar L with Emphasis on Immune Molecules written by Erling Olaf Koppang and published by . This book was released on 1998 with total page 32 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Identification and Annotation of Full length Genes in Atlantic Salmon Salmo Salar written by Jong S. Leong and published by . This book was released on 2010 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Large-scale expressed sequence tags (ESTs) in Atlantic salmon (Salmo salar) are examined to answer questions regarding salmonid transcriptomes. ESTs represent raw and incomplete gene sequences that need to be read, assembled and analyzed with computer software. The goal of this thesis was to develop an automatically curated and publicly accessible set of annotated full-length genes, representing a near-complete transcript set for Salmo salar. In turn, these genes provide the framework for studies in gene expression, conservation, and molecular evolution. The work presented here also touches on the results of a molecular evolution study, as an example of how full-length gene identification can be used to answer biological questions. Previous to this study, a limited number of Atlantic salmon cDNA libraries and ESTs were known. To further the goal of determining complete gene sequences, highly enriched full-length cDNA libraries and full-length libraries were created and sequenced, resulting in the ability to identify a large number of full-length reference genes. Together, all libraries represent a diverse pool of transcriptome sequences for Salmo salar. The goal of producing an accurate large-scale full-length gene set on a duplicated genome is not trivial. Complete systems for this objective do not readily exist. EST sequencing, EST assembly, and data storage, are just a few of the initial computational issues that are addressed. Once these issues are resolved, the multi-step workflow of full-length gene determination is described. The final challenge involving the development of a concise and universally accessible system for visualization is discussed. The resulting computational framework that has been developed is shown to be able to handle the intricacies and the size of a duplicated salmonid genome. It has been largely accepted that Atlantic salmon have undergone a recent genome duplication. Gene paralogs provide one source of evidence for this event. Analysis of paralogs revealed signatures of asymmetric evolution possibly due to relaxation of selective pressure. This thesis provides a complete Bioinformatics analysis pipeline to analyze and to visualize a set of full-length reference genes for Atlantic salmon. Using full-length genes as a framework, the topic of molecular evolution was addressed to show evidence of asymmetrical evolution among gene duplicates. The full-length reference genes, along with ESTs and all putative transcripts, have been made publicly available. These results serve as a valuable genomic resource for next-generation sequencing and for all other salmonid research endeavours.
Download or read book Molecular Biology Frontiers written by Peter W. Hochachka and published by . This book was released on 1993-12 with total page 492 pages. Available in PDF, EPUB and Kindle. Book excerpt: This second volume in the series covers such topics as DNA fingerprinting of fishes, the cytochromes P450 in fish, the molecular biology of bacterial fish diseases, and new insights into the origins of the diversity and distribution of fish antifreeze proteins. The book will be of great value to fisheries scientists, animal biochemists, physiologists and endocrinologists, and aquaculturists. It will provide researchers and students alike with a pertinent information source from theoretical and experimental angles.
Download or read book Molecular Research in Aquaculture written by Ken Overturf and published by John Wiley & Sons. This book was released on 2009-09-23 with total page 406 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular Research in Aquaculture Molecular research and biotechnology have long been fields of study with applications useful to aquaculture and other animal sciences. Molecular Research in Aquaculture looks to provide an understanding of molecular research and its applications to the aquaculture industry in a format that allows individuals without prior experience in this area to learn about and understand this important field. Molecular Research in Aquaculture opens with an introductory chapter giving background information on the aquaculture industry and the development of the science and research methods to what is currently being used. From there it discusses how new, innovative techniques are now being converted and used for research in this field. Introductory chapters on basic molecular biological techniques, such as PCR, cloning, and hybridization, and their rationale provide the foundation for an in-depth look at molecular research and its specific applications. The remaining chapters review key areas of molecular research such as microarray analysis, quantitative PCR, and transgenics. Molecular Research in Aquaculture will be a valuable reference for professionals and researchers with an interest in the development of molecular technologies and their applications to the field of aquaculture. Coverage of basic molecular biological techniques and their rationale In-depth look at molecular research and their applications to aquaculture Valuable reference on the developments of this key area in aquaculture research
Download or read book Identification Evolution and Expression of Three Olfactory Gene Families in Atlantic Salmon Salmo Salar written by Kimberley Anne Johnstone and published by . This book was released on 2011 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: It has been hypothesized that salmonids use olfactory cues to return to their natal rivers and streams. However, the key components of the molecular pathway involved in imprinting and homing are still unknown. If odorants are involved in salmon homing migration then olfactory receptors should play a critical role in the dissipation of information from the environment to the fish. To understand the molecular basis for imprinting and homing in Atlantic salmon (Salmo salar), it is important to identify and characterize the olfactory receptors in the Atlantic salmon genome. Aquatic chemical cues are received through the salmon nares and into the nasal cavity that contains a single olfactory organ, olfactory rosette. The olfactory rosette contains sensory neurons, which are thought to express only one olfactory receptor. In this study, three major superfamilies of fish olfactory receptors (MOR, ora and OlfC) were examined. To identify the olfactory genes in Atlantic salmon several genomic and bioinformatic techniques were used. First, an Atlantic salmon bacterial artificial chromosome (BAC) library was screened with probes designed from previously identified fish olfactory receptor sequences. Then a selected number of hybridization positive BACs containing olfactory receptors were shotgun cloned and sequenced. From these BAC sequences, two ora genes and 55 OlfC genes were identified in Atlantic salmon. The second technique used to identify olfactory receptors in Atlantic salmon was a bioinformatic approach that involved screening a 3-fold Atlantic salmon genome sequence for olfactory receptors. Using this approach, 24 MOR and the remaining five ora genes were identified, as well as another 24 partial genes or pseudogenes. As a first step to understand how olfactory receptors are involved in imprinting and homing, a suite of olfactory receptors were selected to examine the expression profiles of these genes across different life stages and life histories of wild Atlantic salmon from Newfoundland, Canada. Seven differentially expressed OlfC genes were identified in juvenile anadromous salmon compared to returning adult salmon. From this research, I hypothesize that OlfC genes may play an important role in the imprinting of home stream water olfactory cues in anadromous Atlantic salmon.
Download or read book Xenoestrogen Modulated Gene Expression and Development of Molecular Biomarkers in Juvenile Atlantic Salmon written by Fekadu Yadetie and published by . This book was released on 2001 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Atlantic Salmon written by Eric Verspoor and published by John Wiley & Sons. This book was released on 2008-04-15 with total page 520 pages. Available in PDF, EPUB and Kindle. Book excerpt: Atlantic Salmon is a cultural icon throughout its North Atlantic range; it is the focus of probably the World’s highest profile recreational fishery and is the basis for one of the World’s largest aquaculture industries. Despite this, many wild stocks of salmon are in decline and underpinning this is a dearth of information on the nature and extent of population structuring and adaptive population differentiation, and its implications for species conservation. This important new book will go a long way to rectify this situation by providing a thorough review of the genetics of Atlantic salmon. Sponsored by the European Union and the Atlantic Salmon Trust, this book comprises the work of an international team of scientists, carefully integrated and edited to provide a landmark book of vital interest to all those working with Atlantic salmon.
Download or read book Molecular Biology Frontiers written by T.P. Mommsen and published by Elsevier Science. This book was released on 1993-12-01 with total page 492 pages. Available in PDF, EPUB and Kindle. Book excerpt: This second volume in the series covers such topics as DNA fingerprinting of fishes, the cytochromes P450 in fish, the molecular biology of bacterial fish diseases, and new insights into the origins of the diversity and distribution of fish antifreeze proteins. The book will be of great value to fisheries scientists, animal biochemists, physiologists and endocrinologists, and aquaculturists. It will provide researchers and students alike with a pertinent information source from theoretical and experimental angles.
Download or read book Molecular Endocrinology of Fish written by and published by Academic Press. This book was released on 1994-12-23 with total page 547 pages. Available in PDF, EPUB and Kindle. Book excerpt: Hormones have a manifold impact upon growth and metabolism. This book focuses upon the molecular biology of fish hormones and their regulation. Chapters dealing with gonadotropin, corticotropin, vasotocin, isotocin, somatolactin, and other hormones are written by an international team of fish physiologists and endocrinologists. In addition, there are chapters that survey a growing literature on the ways hormones are regulated both in terms of their actions and in terms of the gene transcription that leads to their formation. The first two sections of the book covers brain and pituitary hormones and the latter two sections are devoted to other hormones and their regulation. As more and more endocrinologists and physiologists seek to use hormones that are inexpensive, provide for more facile experimental replication, and are less subject to cumbersome regulation, they will turn to the sorts of fish models reviewed in this book.
Download or read book Rearing Temperature s Effect on the Growth Performance Nutrient Utilization and Immune Response of Growth Hormone Transgenic Female Triploid Atlantic Salmon Salmo Salar written by Eric Ignatz and published by . This book was released on 2019 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: This thesis examined the effect of rearing temperature on AquAdvantage Salmon (AAS; growth hormone transgenic female triploid Atlantic salmon) reared at 10.5°C, 13.5°C, 16.5°C from first feed to 1500 g. Fish reared at 16.5°C reached 1500 g in the shortest amount of time, however, thermal-unit growth coefficient (TGC) results showed that salmon reared at 10.5°C exhibited higher growth rates for most of the trial. Through analysis of whole-body and fillet samples at various growth stages, it was determined that rearing temperature significantly influenced composition, deposition rates and retention efficiencies of numerous nutrients. For example, AAS reared at 16.5°C diverted lipid storage away from muscle tissue into their viscera and were less efficient at retaining important nutrients, such as omega-3 fatty acids. By assessing the antiviral immune gene expression response of fish injected with polyriboinosinic polyribocytidylic acid (pIC), it was concluded that temperature significantly influenced immune responses of AAS. Based on transcript expression responses, as measured by real-time quantitative polymerase chain reaction (qPCR) analysis, it appeared that a more robust immune response was mounted by fish reared at 10.5°C compared to 16.5°C. Collectively, these results help establish foundational knowledge that can guide future research to optimize commercial rearing of AquAdvantage Salmon.
Download or read book Precursor and Gene Structure of a Growth Hormone releasing Hormone like Molecule and Pituitary Adenylate Cyclase Activating Polypeptide from Sockeye Salmon Brain written by David Bernard Parker and published by . This book was released on 1992 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Fish Larval Physiology written by Roderick Nigel Finn and published by CRC Press. This book was released on 2020-04-30 with total page 741 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is intended as a resource for students and researchers interested in developmental biology and physiology and specifically addresses the larval stages of fish. Fish larvae (and fish embryos) are not small juveniles or adults. Rather they are transitionary organisms that bridge the critical gap between the singlecelled egg and sexually immature juvenile. Fish larvae represent the stage of the life cycle that is used for differentiation, feeding and distribution. The book aims at providing a single-volume treatise that explains how fish larvae develop and differentiate, how they regulate salt, water and acid-base balance, how they transport and exchange gases, acquire and utilise energy, how they sense their environment, and move in their aquatic medium, how they control and defend themselves, and finally how they grow up.
Download or read book A Comparative Study of Gene Expression in Wild and Domesticated Atlantic Salmon Salmo Salar L written by Beatrix Bicskei and published by . This book was released on 2015 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Fish Physiology Muscle Development and Growth written by and published by Academic Press. This book was released on 2000-10-31 with total page 336 pages. Available in PDF, EPUB and Kindle. Book excerpt: With the advent of zebrafish as a model system, the development and growth of muscle in fish has become an ever more important process. This volume, in the continuing Fish Physiology series, focuses attention on muscle from the genetics of muscle development to application of muscle growth patterns to aquacultural production.
