Download or read book Molecular Mechanisms of Sea Urchin Egg Activation at Fertilization written by Yama Aref Abassi and published by . This book was released on 1999 with total page 456 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Mechanisms of Egg Activation written by R. Nuccitelli and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 315 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proceedings of the meeting held in Sept. 1988. Research over the past decade has yielded several breakthroughs in this field and most of them are discussed in this volume, including: electrical events accompanying sperm-egg interactions; signal transduction mechanisms in egg activation; metabolic co
Download or read book The Sea Urchin Embryo written by G. Czihak and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 719 pages. Available in PDF, EPUB and Kindle. Book excerpt: Sea urchin eggs are objects of wonder for the student who sees them for the first time under the microscope. The formation of the fertil ization membrane after insemination, the beauty of mitotic cleavage, the elegant swimming of embryos, remain an esthetic pleasure even for the eyes of seasoned investigators. But sea urchin eggs have other, more practical, advantages: they lend themselves to surgical operation without difficulty and they heal perfectly; they can be obtained in very large amounts and represent thus an extremely favorable material for biochemists and molecular embryologists. It is not surprising that, in view of these exceptional advantages, sea urchin eggs have attracted the interest of innumerable biologists since O. HERTWIG discovered the fusion of the pronuclei (amphimixy), in Paracentrotus lividus, almost a century ago. The purpose of the present book is to present, in a complete and orderly fashion, the enormous amount of information which has been gathered, in the course of a hun dred years of sea urchin embryology. JOSEPH NEEDHAM, in 1930, was still able to present all that was known, at that time, on the biochemistry of all possible species of developing eggs and embryos in his famous "Chemical Embryology" (Cambridge University Press) . It would no longer be possible for one man to write a modern version of what was a "Bible" for the young embryologists of forty years ago.
Download or read book Molecular Biology of The Cell written by Bruce Alberts and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Sea Urchin Embryo written by G. Giudice and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 250 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book should be regarded as the continuation to my previous book Developmental Biology of the Sea Urchin Embryo, edited by the Academic Press in 1973, rather than as a new edition. Due to the exceedingly high rate of development in this field (something like 2000 papers have been published on this subject in these last 10 years), I preferred, in fact, not to describe again in detail the enormous amount of the old literature, as was attempted in my previous book, but to briefly summarize the state of the art in each problem and to describe in some detail the experiments per formed in the last 12 years. In doing so, more emphasis was given to the more recent ones and to those which can be considered as corner stones in each subject. Care was, however, taken to mention the reviews or key papers in which the reader can find a source of the details of the older literature, besides refering him to my previous book.
Download or read book Sea Urchin Sperm Chemotaxis written by Yasmeen H. Hussain and published by . This book was released on 2016 with total page 115 pages. Available in PDF, EPUB and Kindle. Book excerpt: Egg chemoattraction of conspecific sperm mediates fertilization, a critical juncture in reproduction, especially in broadcast-spawning organisms like sea urchins. In the century that sea urchin sperm chemotaxis was studied before I started my work, many discoveries were made about the variety of peptide attractants that sea urchin eggs release into the ocean environment and the molecular mechanisms of the sperm chemotactic response. However, many questions were left unanswered. Particularly, previous work has focused on the patterns that persist across populations - it is well-known that chemotaxis generally changes sperm behavior and increases recruitment to eggs. In contrast, the differences between female and male sea urchin individuals and how those differences affect the dynamics of chemoattraction and chemotaxis has not been well-studied. In this dissertation, I studied not only average behavior but also individual differences. I (i) investigated whether differences in sperm chemotactic ability between individual males translated to differences in fertilization success, (ii) measured the chemoattractant release from individual females in the framework of their ability to attract sperm, and (iii) studied the behavior and physiology of sperm in specific chemoattractant gradients to understand their threshold of recruitment and fertilization-relevant response. I used an enzyme to digest chemoattractant and measured the resulting difference in the percent of eggs fertilized, then used novel chemotaxis assays in microfluidic channels to compare individual male chemotactic performance to fertilization success. Through this study, I found that chemotaxis can explain differences in individual male fertilization success. I then used high-performance liquid chromatography to determine the amount of chemoattractant released by eggs of individual females, computational models to investigate the resulting gradients, and microfluidic chemotaxis assays to assess how variable gradients affect sperm behavior. Through this work, I found that individual females vary significantly in their attractant release, which can in turn significantly affect sperm’s ability to detect their chemoattractant gradients. Lastly, I used microfluidic chemotaxis assays coupled with calcium imaging tools to record concurrent sperm behavioral and physiologic responses to gradients of chemoattractant and applied data analytics methodologies to determine the amount of attractant needed to recruit sperm, and found a surprising amount of variation in individual sperm behavior. In summary, my dissertation is an investigation of sperm chemotaxis in the frame of the individual: how individual male fertilization success correlates with their sperm chemotactic ability, how differences between females in egg chemoattractant release change their surrounding chemoattractant gradients, and how variable chemoattractant gradients affect the behavior and physiology of aggregate and individual sperm.
Download or read book Analysis of the Surface Structures of the Sea Urchin Egg by Means of Antibodies written by Peter Perlmann and published by . This book was released on 1957 with total page 32 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Development of Sea Urchins Ascidians and Other Invertebrate Deuterostomes Experimental Approaches written by Charles A. Ettensohn and published by Gulf Professional Publishing. This book was released on 2004-10-22 with total page 948 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a practical guide to experimental methods for studying the development of invertebrate deuterostomes, such as sea urchins, ascidians, hemichordates, and amphioxus. These model organisms are of contemporary and historical importance to the study of developmental biology, particularly genomic research. The chapters provide detailed experimental protocols that cover a broad range of topics in modern experimental methods. Topics covered range from rearing embryos to the care of adult animals, while also presenting the basic experimental methods including light and electron microscopy, used to study gene expression, transgenics, reverse genetics, and genomic approaches. * Covers a wide range of methods, from classical embryology through modern genomics * Discusses animals related to vertebrates, providing a valuable evolutionary perspective * Includes a practical guide to the use of sea urchins in the teaching laboratory
Download or read book Sexual Reproduction in Animals and Plants written by Hitoshi Sawada and published by Springer. This book was released on 2014-02-07 with total page 463 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book contains the proceedings of the International Symposium on the Mechanisms of Sexual Reproduction in Animals and Plants, where many plant and animal reproductive biologists gathered to discuss their recent progress in investigating the shared mechanisms and factors involved in sexual reproduction. This now is the first book that reviews recent progress in almost all fields of plant and animal fertilization. It was recently reported that the self-sterile mechanism of a hermaphroditic marine invertebrate (ascidian) is very similar to the self-incompatibility system in flowering plants. It was also found that a male factor expressed in the sperm cells of flowering plants is involved in gamete fusion not only of plants but also of animals and parasites. These discoveries have led to the consideration that the core mechanisms or factors involved in sexual reproduction may be shared by animals, plants and unicellular organisms. This valuable book is highly useful for reproductive biologists as well as for biological scientists outside this field in understanding the current progress of reproductive biology.
Download or read book Fertilization in Protozoa and Metazoan Animals written by Juan J. Tarin and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 329 pages. Available in PDF, EPUB and Kindle. Book excerpt: Reproduction is the origination of new organisms from pre-existing ones. Among more than 35 separated forms of reproduction including several types of gamogony, parthenogenesis, agamogenesis, fission and division, and plas motomy, the bisexual mode of reproduction via fertilization provides genetic variability that allows species to adapt quickly to competitive and constantly changing environments. Several excellent reviews and books have been written in the past to analyse the mechanisms of fertilization in different eukaryotic species. During the last few years, however, renewed attention has been paid to examining the process of oocyte fertilization at the cellular/molecular level not only within a single species/group but also through different phylogenetic lineages. As a result of this effort, knowledge of the molecular pathways used by oocytes and spermatozoa at fertilization has increased, but still many ques tions remain to be answered. Being aware of the necessity of providing an inte grated view of the process of fertilization, this book has been entirely devoted to reviewing the process of oocyte fertilization at the cellular/molecular level in two different and separated groups of eukaryotic organisms: protozoa and metazoan animals. The book is organized into six sections dealing with oocyte fertilization in protozoa, invertebrates, teleost fishes, amphibians, birds and mammals. These sections are followed by a summary/concluding chapter that provides a com parative overview of the process of fertilization in these groups of eukaryotes.
Download or read book The Molecular Mechanisms Underlying Exocytosis in Sea Urchin Eggs written by Julia Catherine Avery and published by . This book was released on 1996 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Encyclopedia of Reproduction written by and published by Academic Press. This book was released on 2018-06-29 with total page 3905 pages. Available in PDF, EPUB and Kindle. Book excerpt: Encyclopedia of Reproduction, Second Edition, Six Volume Set comprehensively reviews biology and abnormalities, also covering the most common diseases in humans, such as prostate and breast cancer, as well as normal developmental biology, including embryogenesis, gestation, birth and puberty. Each article provides a comprehensive overview of the selected topic to inform a broad spectrum of readers, from advanced undergraduate students, to research professionals. Chapters also explore the latest advances in cloning, stem cells, endocrinology, clinical reproductive medicine and genomics. As reproductive health is a fundamental component of an individual’s overall health status and a central determinant of quality of life, this book provides the most extensive and authoritative reference within the field. Provides a one-stop shop for information on reproduction that is not available elsewhere Includes extensive coverage of the full range of topics, from basic, to clinical considerations, including evolutionary advances in molecular, cellular, developmental and clinical sciences Includes multimedia and interactive teaching tools, such as downloadable PowerPoint slides, video content and interactive elements, such as the Virtual Microscope
Download or read book Textbook of Clinical Embryology written by Kevin Coward and published by Cambridge University Press. This book was released on 2013-10-31 with total page 408 pages. Available in PDF, EPUB and Kindle. Book excerpt: The success of Assisted Reproductive Technology is critically dependent upon the use of well optimized protocols, based upon sound scientific reasoning, empirical observations and evidence of clinical efficacy. Recently, the treatment of infertility has experienced a revolution, with the routine adoption of increasingly specialized molecular biological techniques and advanced methods for the manipulation of gametes and embryos. This textbook – inspired by the postgraduate degree program at the University of Oxford – guides students through the multidisciplinary syllabus essential to ART laboratory practice, from basic culture techniques and micromanipulation to laboratory management and quality assurance, and from endocrinology to molecular biology and research methods. Written for all levels of IVF practitioners, reproductive biologists and technologists involved in human reproductive science, it can be used as a reference manual for all IVF labs and as a textbook by undergraduates, advanced students, scientists and professionals involved in gamete, embryo or stem cell biology.
Download or read book The Fish Oocyte written by Patrick J. Babin and published by Springer Science & Business Media. This book was released on 2007-08-20 with total page 513 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book addresses the growing needs in deciphering the biological processes associated with fish reproduction, in view of the growth of aquaculture and the dwindling natural stocks of commercially important fish. It presents a comprehensive overview on egg production in fish, from the standpoint of the oocyte. With this view in mind, the book includes chapters on oocyte development (oogenesis), hormonal regulation and hormone receptors, formation of the egg envelopes, growth, accumulation of nutrients and maternal transcripts, maturation, hydration, ovulation and fertilization. A special emphasis is placed on using state-of-the-art tools including electron microscopy for discerning the ultra-structure of the follicle and genomic/proteomic tools to fully understand biological basis of fish reproduction. Studies on promoting oocyte maturation, ovulation and spawning in farmed fish and preservation of fish oocytes at low temperatures are also included. The book will appeal to University lecturers, students, research scientists and those associated with culture of fish in freshwater or marine aquaculture.
Download or read book Fertilization written by Daniel M. Hardy and published by Academic Press. This book was released on 2002 with total page 456 pages. Available in PDF, EPUB and Kindle. Book excerpt: Gamete reansport / Susan S. Suarez -- Sperm motility activation and chemoattraction / Timothy A. Quill and David L. Garbers -- Capacitation / Bijay S. Jaiswal and Michael Eisenbach -- Function of the egg's extracellular matrix / Richard A. Cardullo and Catherine D. Thaler -- Sperm adhesion to the extracellular / Ming Bi, Michael J. Wassler, and Daniel M. Hardy -- Signal transduction mechanisms regulating sperm acrosomal exocytosis / Gregory S. Kopf -- Regulation of sperm ion currents / Alberto Darszon, Felipe Espinosa, Blanca Galindo, Daniel Sánchez, and Carmen Beltrán -- Function of the sperm acrosome/ George L. Gerton -- Gamete fusion in mammals / Paul Primakoff and Diana G. Myles -- Membrane events of egg activation / Karl Swann and Keith T. Jones -- Molecular genetics of fertilization / Patricia Olds-Clarke and Stephen H. Pilder -- Gemete immunobiology / M.G. O'rand and I.A. Lea -- Fertilization biophysics / D.P.L. Green -- Applications of fertility regulation for the management of ...
Download or read book Fertilization and Embryonic Development In Vitro written by Luigi Mastroianni and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 373 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Echinoderms Part B written by and published by Academic Press. This book was released on 2019-04-02 with total page 552 pages. Available in PDF, EPUB and Kindle. Book excerpt: Echinoderms, Volume 151, the latest release in the Methods in Cell Biology series, highlights advances in the field, with this update presenting chapters on Echinoderm Genome Databases, analysis of gene regulatory networks, using ATAC-seq and RNA-seq to increase resolution in GRN connectivity, multiplex cis-regulatory analysis, experimental approaches GRN/signal pathways, BACs, analysis of chromatin accessibility using ATAC-seq, analysis of sea urchin proteins /Click IT, CRISPR/Cas9-mediated genome editing in sea urchins, super-resolution and in toto imaging of echinoderm embryos, and methods for analysis of intracellular ion signals in sperm, eggs and embryos. Presents clear, concise protocols provided by experts who have established the echinoderms as a model systems Highlights new advances in the field, with this update presenting interesting chapters on echinoderms
