Download or read book Molecular Genetic Analysis of the Rfa Locus of Escherichia Coli K 12 and Its Involvement in the Assembly of Lipopolysaccharide O Antigen written by John David Klena and published by . This book was released on 1993 with total page 426 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book American Doctoral Dissertations written by and published by . This book was released on 1993 with total page 704 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Dissertation Abstracts International written by and published by . This book was released on 1994 with total page 746 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Cumulated Index Medicus written by and published by . This book was released on 1985 with total page 1104 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Endotoxin in Health and Disease written by Helmut Brade and published by CRC Press. This book was released on 2020-10-28 with total page 968 pages. Available in PDF, EPUB and Kindle. Book excerpt: Offering a basis for further research into the interactions of hosts and pathogens, this work gathers up-to-date findings, and details basic structures, functions and immunology. It provides descriptions of a variety of experimental endotoxin neutralizing agents, as well as a guide to clinical research initiatives and the latest treatments.
Download or read book Applied and Environmental Microbiology written by and published by . This book was released on 2000 with total page 998 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Identification and Characterization of the GmhA Gene Used for Inner Core Lipopolysaccharide Synthesis in Escherichia Coli written by Joanna S. Brooke and published by . This book was released on 1996 with total page 400 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Genetic Analysis of Lipopolysaccharide Core Biosynthesis by Escherichia Coli K 12 written by Craig T. Parker and published by . This book was released on 1992 with total page 268 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Genetic and Molecular Analysis of the RecR Locus of Escherichia Coli K 12 written by Akeel Abdulla Mahdi and published by . This book was released on 1991 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book A Genetic Analysis of the TolA B Locus of Escherichia Coli K 12 written by Alan Bernstein and published by . This book was released on 1972 with total page 184 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book An Investigation of the Genetic Control of Biofilm Formation in Bacteria E Coli K 12 MG1655 written by Mohd Adnan and published by . This book was released on 2012 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The ability to adapt to changing environments is essential for survival. Bacteria have developed sophisticated means by which they sense and respond to stresses imposed by changes in the environment. Escherichia coli (E. coli) have served as a model organism for studies in molecular genetics and physiology since the 1960s. I have undertaken this study and address three outstanding questions. Firstly, the involvement of morphogene bolA and RNA polymerase sigma factor (rpoS) in biofilm formation. Secondly, the effect on respiratory activity of E. coli in presence and absence of these two genes and thirdly, the adherence pattern and formation of biofilm by E. coli on stainless steel, polypropylene and silicon surfaces under various stress-induced conditions. Bacterial biofilms are structural assemblages of microbial cells that encase themselves in a protective self produced matrix and irreversibly attach to surface. Their intense resistance to antibiotic and various environmental stresses has implicated them as playing a possible role in the pathogenesis of many chronic diseases. Although, the role of rpoS and bolA genes in long term stationary phase growth conditions and their response to it is now well-known, their objective presence and importance in short term response to different environmental cues which may lead to biofilm formation remains unknown. The rpoS gene encodes a stationary phase specific sigma factor of RNA polymerase and is a key regulator of E. coli stationary phase responses. It has been experiential under laboratory conditions that gene expression is induced by stressful environmental conditions and certain metabolic intermediates. Various stress environments were employed both in planktonic and biofilm phases to examine the sudden response of rpoS against different environmental conditions. However, it was observed that sudden rpoS response varies from stress to stress conditions. The gene bolA has been shown to trigger the formation of round cells when over expressed in stationary phase. From this research, it is concluded that bolA is not only confined to stationary phase, it also involves in biofilm formation under stress environments and essential for normal cell morphology. It also plays a major role in respiration and attachment of E. coli under diverse environmental stress surroundings. The main objective of this study was to understand the impact of heat, cold, acid and hydrogen peroxide on E. coli K-12 MG1655 and its stress response in presence and absence of rpoS and bolA genes. E. coli cells were exposed to sub-lethal levels of each stress for 15 minutes in both planktonic and biofilm phases and post-stress response i.e. gene expression level was evaluated. A real-time reverse transcription polymerase chain reaction (RT-PCR) assay, using the Applied Biosystems 7500? real-time cycler, was developed for the purpose of this investigation of rpoS and bolA genes transcription. The assay was used specifically to quantify rpoS and bolA mRNA levels; however the method can readily be applied to the study of other E. coli genes. The method was uniquely applied to the investigation of these two genes throughout the growth cycle of E. coli in planktonic and biofilm phase in LB broth, in order to ascertain the patterns of expression for these genes. Scanning electron microscopy (SEM) was used for direct examining the cell attachment and biofilm formation on various surfaces under different stress conditions. In summary, this thesis embodies research investigating the role of rpoS and bolA genes in E. coli K-12 MG1655 biofilm formation and provides further evidence, that bacterial biofilms play a major role in resistance to various environmental cues.
Download or read book The Genus Yersinia written by Robert D. Perry and published by Springer Science & Business Media. This book was released on 2007-09-25 with total page 454 pages. Available in PDF, EPUB and Kindle. Book excerpt: The 9th International Symposium on Yersinia was held in Lexington, Kentucky, USA on October 10-14, 2006. Over 250 Yersinia researchers from 18 countries gathered to present and discuss their research. In addition to 37 oral presentations, there were 150 poster presentations. This Symposium volume is based on selected presentations from the meeting and contains both reviews and research articles. It is divided into six topic areas: 1) genomics; 2) structure and metabolism; 3) regulatory mechanisms; 4) pathogenesis and host interactions; 5) molecular epidemiology and detection; and 6) vaccine and antimicrobial therapy development. Consequently, this volume covers a wide range of current research areas in the Yersinia field.
Download or read book Molecular Cloning and Analysis of the Ruv Gene of Escherichia Coli K12 written by F. E. Benson and published by . This book was released on 1988 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Perfect Slime written by Hans-Curt Flemming and published by IWA Publishing. This book was released on 2016-09-15 with total page 336 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Perfect Slime presents the latest state of knowledge and all aspects of the Extracellular Polymeric Substances, (EPS) matrix – from the ecological and health to the antifouling perspectives. The book brings together all the current material in order to expand our understanding of the functions, properties and characteristics of the matrix as well as the possibilities to strengthen or weaken it. The EPS matrix represents the immediate environment in which biofilm organisms live. From their point of view, this matrix has paramount advantages. It allows them to stay together for extended periods and form synergistic microconsortia, it retains extracellular enzymes and turns the matrix into an external digestion system and it is a universal recycling yard, it protects them against desiccation, it allows for intense communication and represents a huge genetic archive. They can remodel their matrix, break free and eventually, they can use it as a nutrient source. The EPS matrix can be considered as one of the emergent properties of biofilms and are a major reason for the success of this form of life. Nevertheless, they have been termed the “black matter of biofilms” for good reasons. First of all: the isolation methods define the results. In most cases, only water soluble EPS components are investigated; insoluble ones such as cellulose or amyloids are much less included. In particular in environmental biofilms with many species, it is difficult to impossible isolate, separate the various EPS molecules they are encased in and to define which species produced which EPS. The regulation and the factors which trigger or inhibit EPS production are still very poorly understood. Furthermore: bacteria are not the only microorganisms to produce EPS. Archaea, Fungi and algae can also form EPS. This book investigates the questions, What is their composition, function, dynamics and regulation? What do they all have in common?
Download or read book Bacterial Lipopolysaccharides written by Yuriy A. Knirel and published by Springer Science & Business Media. This book was released on 2011-07-13 with total page 444 pages. Available in PDF, EPUB and Kindle. Book excerpt: The bacterial lipopolysaccharide also known as endotoxin is exhaustively covered in the present work. Central emphasis is placed upon the fine chemical structure of the lipopolysaccharide and its significance for understanding their activity and function. In particular, the role it plays in the interaction of bacteria with other biological systems is examined. New aspects of their physicochemical biology are introduced and updates to the current knowledge concerning the lipopolysaccharide are provided. This important class of biomolecules has recently attracted the attention of many investigators, in particular for understanding its involvement in innate immunity, toll-like receptor recognition and intracellular signaling.
Download or read book The TolC Locus of Escherichia Coli K 12 written by Renato Morona and published by . This book was released on 1982 with total page 230 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The BAM Complex written by Susan Buchanan and published by Humana. This book was released on 2015-10-02 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume is comprised of a collection of experimental protocols for common techniques and strategies used to study the biogenesis of b-barrel outer membrane proteins in Gram-negative bacteria. The BAM Complex: Methods and Protocols guides readers through methods on the function of the BAM complex, the roles played by each of the individual components, the expression and purification of the components, crystallization and structure determination of the components, and how the individual Bam components may assemble into a functional complex. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, The BAM Complex: Methods and Protocols will serve as an invaluable reference for those interested in studying the BAM complex.
