Download or read book Molecular Characterization of the Gene MRNAS Precursor Proteins and Mature Subunits Involved in the Synthesis of the NADP specific Glutamate Dehydrogenase Isoenzymes in Chlorella Sorokiniana written by Philip Miller and published by . This book was released on 1994 with total page 354 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Purification and Partial Kinetic and Physical Characterization of Two NADP specific Glutamate Dehydrogenase Isoenzymes and Their Protein Precursors and Measurement of the Patterns of Accumulation and Rates of Degradation of Their Nonidentical Subunits in Synchronized Cells of Chlorella Cultured in Different Concentrations of Ammonia written by Newell F. Bascomb and published by . This book was released on 1985 with total page 290 pages. Available in PDF, EPUB and Kindle. Book excerpt: Two ammonium-inducible, chloroplast-localized, NADP-specif ic glutamate dehydrogenases were purified from Chlorella sorokiniana . They were homopolymers of either alpha or beta subunits with molecular weights of 55,500 and 53,000, respectively. Purification was achieved by gel filtration (Sephacryl S-300), fast protein liquid chromatography (Pharmacia, Mono Q column), and substrate affinity chromatography (Pharmacia, NADP-Sepharose 4B, Type 4). These isoenzymes were separated by their differential binding to the substrate affinity column. Peptide mapping of purified alpha and beta subunits showed them to have a high degree of sequence homology. Because the ammonia K of alpha holoenzyme was sensitive to changes in NADPH concentration, its K ranged from 0.02 mM to 3.5 mM. With m changes in NADPH concentration, the aminonia K^ of beta holoenzyme remained constant at 70 mM. By use of SDS slab-gel electrophoresis and a Western blot/immunodetection procedure, patterns of accumulation of alpha and beta subunits (in their holoenzyme) were measured in cells cultured in media, containing different concentrations of ammonia. Pulse-chase experiments with Ssulfate were performed to measure the rates of degradation of the two isoenzymes. When the culture medium contained 2 mM ammonia or lower, cells accumulated only the alpha holoenzyme. Above 2 mM ammonia, cells contained both isoenzymes; however, their patterns of accumulation and rates of degradation were very different. The physiological role of alpha and beta holoenzymes appears to be ammonia assimilation at low and high external ammonia concentrations, respectively. From in vitro translation studies with total cellular poly(A)"^ RNA, isolated from cells engaged in synthesis of alpha or beta holoenzymes or both, it was concluded that alpha and beta subunits have protein precursor(s) of identical molecular weight (M = 58,500). When the putative protein-precursor (s) were incubated in vitro, with cell-free extracts from Chlorella cells, they were processed to proteins the size of alpha and beta subunits. From in vitro processing experiments, it was concluded that either two different precursors of the same molecular weight are specifically processed to give alpha and beta subunits, or the specificity or type of processing-enzyme changes to give the two subunits from a single precursor -protein.
Download or read book Cumulated Index Medicus written by and published by . This book was released on 1998 with total page 1852 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Photosynthetic Nitrogen Assimilation and Associated Carbon and Respiratory Metabolism written by C.H. Foyer and published by Springer Science & Business Media. This book was released on 2006-04-11 with total page 300 pages. Available in PDF, EPUB and Kindle. Book excerpt: According to many textbooks, carbohydrates are the photosynthesis and mitochondrial respiration fluctuate in a circadian manner in almost every unique final products of plant photosynthesis. However, the photoautotrophic production of organic organism studied. In addition, external triggers and environmental influences necessitate precise and nitrogenous compounds may be just as old, in appropriate re-adjustment of relative flux rates, to evolutionary terms, as carbohydrate synthesis. In the algae and plants of today, the light-driven assimilation prevent excessive swings in energy/resource provision of nitrogen remains a key function, operating and use. This requires integrated control of the alongside and intermeshing with photosynthesis and expression and activity of numerous key enzymes in respiration. Photosynthetic production of reduced photosynthetic and respiratory pathways, in order to carbon and its reoxidation in respiration are necessary co-ordinate carbon partioning and nitrogen assim- ation. to produce both the energy and the carbon skeletons required for the incorporation of inorganic nitrogen This volume has two principal aims. The first is to into amino acids. Conversely, nitrogen assimilation provide a comprehensive account of the very latest developments in our understanding of how green is required to sustain the output of organic carbon cells reductively incorporate nitrate and ammonium and nitrogen. Together, the sugars and amino acids into the organic compounds required for growth.
Download or read book Aquatic Sciences and Fisheries Abstracts written by and published by . This book was released on 1994-11 with total page 402 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Grapevine Molecular Physiology Biotechnology written by Kalliopi A. Roubelakis-Angelakis and published by Springer Science & Business Media. This book was released on 2009-06-04 with total page 636 pages. Available in PDF, EPUB and Kindle. Book excerpt: Grapevine is one of the most widely cultivated plant species worldwide. With the publication of the grapevine genome sequence in 2007, a new horizon in grapevine research has unfolded. Thus, we felt that a new edition of ‘Molecular Biology & Biotechnology of the Grapevine’ could expand on all the latest scientific developments. In this edition and with the aid of 73 scientists from 15 countries, ten chapters describe new aspects of Grapevine Molecular Physiology and Biotechnology and eleven chapters have been revised and updated. This book is intended to be a reference book for researchers, scientists and biotechnological companies, who want to be updated in viticultural research, but also it can be used as a textbook for graduate and undergraduate students, who are interested in the Molecular Biology and Biotechnology of Plants with an emphasis on the Grapevine.
Download or read book Algal Adaptation to Environmental Stresses written by L.C. Rai and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 428 pages. Available in PDF, EPUB and Kindle. Book excerpt: Algae, generally held as the principal primary producers of aquatic systems, inhabit all conceivable habitats. They have great ability to cope with a harsh environment, e.g. extremely high and low temperatures, suboptimal and supraoptimal light intensities, low availability of essential nutrients and other resources, and high concentrations of toxic chemicals, etc. A multitude of physiological, biochemical, and molecular strategies enable them to survive and grow in stressful habitats. This book presents a critical account of various mechanisms of stress tolerance in algae, many of which may occur in microbes and plants as well.
Download or read book Plant Abiotic Stress Tolerance written by Mirza Hasanuzzaman and published by Springer. This book was released on 2019-04-04 with total page 506 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plants have to manage a series of environmental stresses throughout their entire lifespan. Among these, abiotic stress is the most detrimental; one that is responsible for nearly 50% of crop yield reduction and appears to be a potential threat to global food security in coming decades. Plant growth and development reduces drastically due to adverse effects of abiotic stresses. It has been estimated that crop can exhibit only 30% of their genetic potentiality under abiotic stress condition. So, this is a fundamental need to understand the stress responses to facilitate breeders to develop stress resistant and stress tolerant cultivars along with good management practices to withstand abiotic stresses. Also, a holistic approach to understanding the molecular and biochemical interactions of plants is important to implement the knowledge of resistance mechanisms under abiotic stresses. Agronomic practices like selecting cultivars that is tolerant to wide range of climatic condition, planting date, irrigation scheduling, fertilizer management could be some of the effective short-term adaptive tools to fight against abiotic stresses. In addition, “system biology” and “omics approaches” in recent studies offer a long-term opportunity at the molecular level in dealing with abiotic stresses. The genetic approach, for example, selection and identification of major conditioning genes by linkage mapping and quantitative trait loci (QTL), production of mutant genes and transgenic introduction of novel genes, has imparted some tolerant characteristics in crop varieties from their wild ancestors. Recently research has revealed the interactions between micro-RNAs (miRNAs) and plant stress responses exposed to salinity, freezing stress and dehydration. Accordingly transgenic approaches to generate stress-tolerant plant are one of the most interesting researches to date. This book presents the recent development of agronomic and molecular approaches in conferring plant abiotic stress tolerance in an organized way. The present volume will be of great interest among research students and teaching community, and can also be used as reference material by professional researchers.
Download or read book Gasotransmitters in Plants written by Lorenzo Lamattina and published by Springer. This book was released on 2016-09-01 with total page 334 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book describes the three gasotransmitters nitric oxide (NO), hydrogen sulphide (H2S) and carbon monoxide (CO) and their function as intracellular signalling molecules in plants. Common properties are shared by NO, H2S and CO: they are beneficial at low concentrations but hazardous in higher amounts; they are small molecules of gas; they can freely cross cell membranes; their effects do not rely on receptors; they are generated enzymatically and their production is regulated; their functions can be mimicked by exogenous application; and their cellular effects may or may not be mediated by second messengers, but have specific cellular and molecular targets. In plants, many aspects of the biology of gasotransmitters remain completely unknown and generate intriguing questions, which will be discussed in this book.
Download or read book Microorganisms in Environmental Management written by T. Satyanarayana and published by Springer Science & Business Media. This book was released on 2012-01-02 with total page 827 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microbes and their biosynthetic capabilities have been invaluable in finding solutions for several intractable problems mankind has encountered in maintaining the quality of the environment. They have, for example, been used to positive effect in human and animal health, genetic engineering, environmental protection, and municipal and industrial waste treatment. Microorganisms have enabled feasible and cost-effective responses which would have been impossible via straightforward chemical or physical engineering methods. Microbial technologies have of late been applied to a range of environmental problems, with considerable success. This survey of recent scientific progress in usefully applying microbes to both environmental management and biotechnology is informed by acknowledgement of the polluting effects on the world around us of soil erosion, the unwanted migration of sediments, chemical fertilizers and pesticides, and the improper treatment of human and animal wastes. These harmful phenomena have resulted in serious environmental and social problems around the world, problems which require us to look for solutions elsewhere than in established physical and chemical technologies. Often the answer lies in hybrid applications in which microbial methods are combined with physical and chemical ones. When we remember that these highly effective microorganisms, cultured for a variety of applications, are but a tiny fraction of those to be found in the world around us, we realize the vastness of the untapped and beneficial potential of microorganisms. At present, comprehending the diversity of hitherto uncultured microbes involves the application of metagenomics, with several novel microbial species having been discovered using culture-independent approaches. Edited by recognized leaders in the field, this penetrating assessment of our progress to date in deploying microorganisms to the advantage of environmental management and biotechnology will be widely welcomed.
Download or read book Bibliography of Agriculture with Subject Index written by and published by . This book was released on 1995 with total page 480 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Class 1 Oxidoreductases IX written by Dietmar Schomburg and published by Springer Science & Business Media. This book was released on 2005-05-20 with total page 752 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Springer Handbook of Enzymes provides concise data on some 5,000 enzymes sufficiently well characterized – and here is the second, updated edition. Their application in analytical, synthetic and biotechnology processes as well as in food industry, and for medicinal treatments is added. Data sheets are arranged in their EC-Number sequence. The new edition reflects considerable progress in enzymology: the total material has more than doubled, and the complete 2nd edition consists of 39 volumes plus Synonym Index. Starting in 2009, all newly classified enzymes are treated in Supplement Volumes.
Download or read book Progress in Botany written by K. Esser and published by Springer Science & Business Media. This book was released on 1999-11-12 with total page 396 pages. Available in PDF, EPUB and Kindle. Book excerpt: With one volume each year, this series keeps scientists and advanced students informed of the latest developments and results in all areas of the plant sciences. The present volume includes reviews on genetics, cell biology, and vegetation science.
Download or read book Progress in Botany Vol 81 written by Francisco M. Cánovas and published by Springer Nature. This book was released on 2020-01-23 with total page 407 pages. Available in PDF, EPUB and Kindle. Book excerpt: With one volume each year, this series keeps scientists and advanced students informed of the latest developments and results in all areas of the plant sciences. The present volume includes reviews on plant physiology, biochemistry, genetics and genomics, forests, and ecosystems.
Download or read book Sulfur Metabolism in Phototrophic Organisms written by Rüdiger Hell and published by Springer Science & Business Media. This book was released on 2008-03-19 with total page 539 pages. Available in PDF, EPUB and Kindle. Book excerpt: Sulfur is one of the most versatile elements in life. This book provides, for the first time, in-depth and integrated coverage of the functions of sulfur in phototrophic organisms including bacteria, plants and algae. It bridges gaps between biochemistry and cellular biology of sulfur in these organisms, and of biology and environments dominated by them. The book therefore provides a comprehensive overview of plant sulfur relations from genome to environment.
Download or read book Post Transcriptional Control of Gene Expression in Plants written by Witold Filipowicz and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 419 pages. Available in PDF, EPUB and Kindle. Book excerpt: A recent volume of this series (Signals and Signal Transduction Pathways in Plants (K. Palme, ed.) Plant Molecular Biology 26, 1237-1679) described the relay races by which signals are transported in plants from the sites of stimuli to the gene expression machinery of the cell. Part of this machinery, the transcription apparatus, has been well studied in the last two decades, and many important mechanisms controlling gene expression at the transcriptional level have been elucidated. However, control of gene expression is by no means complete once the RNA has been produced. Important regulatory devices determine the maturation and usage of mRNA and the fate of its translation product. Post-transcriptional regulation is especially important for generating a fast response to environmental and intracellular signals. This book summarizes recent progress in the area of post-transcriptional regulation of gene expression in plants. 18 chapters of the book address problems of RNA processing and stability, regulation of translation, protein folding and degradation, as well as intracellular and cell-to-cell transport of proteins and nucleic acids. Several chapters are devoted to the processes taking place in plant organelles.
Download or read book Pathophysiology of Heart Failure written by Naranjan S. Dhalla and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 578 pages. Available in PDF, EPUB and Kindle. Book excerpt: Pathophysiology of Heart Failure brings together leading basic scientists and clinicians, presenting new approaches to this complex problem, involving cardiomyopathic processes and ischemia perfusion injury. The result is a synthesis of state-of-the-art information on molecular biology, cellular physiology and structure-function relationships in the cardiovascular system. The role which excess intracellular calcium plays in the genesis of cardiac dysfunction is described as a fundamental mechanism underlying heart failure; one which may lead to improved prevention and treatment. Audience: Clinical and experimental cardiologists will find the book a helpful source of ideas and inspiration.
