Download or read book Molecular Characterization of Porcine Reproductive and Respiratory Syndrome Virus written by Xiang-Jin Meng and published by . This book was released on 1995 with total page 454 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Molecular Characterization of the Chinese Isolates of Porcine Reproductive and Respiratory Syndrome Virus PRRSV and the Construction and Characterization of the DNA Vaccines written by Yue-ling Wong (M.Phil.) and published by . This book was released on 2001 with total page 274 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Molecular Characterization of the Chinese Isolates of Porcine Reproductive and Respiratory Syndrome Virus Prrsv and the Construction and Characterization of the DNA Vaccines written by Knowledge by Design Yue-Ling Wong and published by Open Dissertation Press. This book was released on 2017-01-27 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: This dissertation, "Molecular Characterization of the Chinese Isolates of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) and the Construction and Characterization of the DNA Vaccines" by Yue-ling, Wong, 王如玲, was obtained from The University of Hong Kong (Pokfulam, Hong Kong) and is being sold pursuant to Creative Commons: Attribution 3.0 Hong Kong License. The content of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation. All rights not granted by the above license are retained by the author. DOI: 10.5353/th_b3122689 Subjects: Swine - Virus diseases Viral proteins DNA vaccines

Download or read book Molecular Characterization Studies of the Porcine Reproductive and Respiratory Syndrome Virus microform written by Sarah Wootton and published by National Library of Canada = Bibliothèque nationale du Canada. This book was released on 2003 with total page 246 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Molecular Characterization of Newly Emerging European like Type 1 Porcine Reproductive and Respiratory Syndrome Virus Isolates in the United States written by Ying Fang and published by . This book was released on 2004 with total page 328 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Molecular Characterization and Co Infection of North American and European Porcine Reproductive and Respiratory Syndrome Virus in Hongkong written by Yick-Yeung Li and published by Open Dissertation Press. This book was released on 2017-01-27 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: This dissertation, "Molecular Characterization and Co-infection of North American and European Porcine Reproductive and Respiratory Syndrome Virus in HongKong" by 李亦揚, Yick-yeung, Li, was obtained from The University of Hong Kong (Pokfulam, Hong Kong) and is being sold pursuant to Creative Commons: Attribution 3.0 Hong Kong License. The content of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation. All rights not granted by the above license are retained by the author. Abstract: Abstract of thesis entitled MOLECULAR CHARACTERIZATION AND CO-INFECTION OF NORTH AMERICAN AND EUROPEAN PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS IN HONG KONG Submitted by Yick Yeung LI for the Degree of Doctor of Philosophy at The University of Hong Kong in March 2008 Porcine reproductive and respiratory syndrome (PPRS), one of the most problematic swine diseases, has been widely spread in most of the swine-producing countries. This disease, characterized by respiratory weakness and reproductive failure was identified in Hong Kong and a previous study revealed the existence of both North American (NA) and European (EU) genotypes in local pig farms, which is rare elsewhere. Therefore, an in-depth study on molecular epidemiology, genomic characterization, prevalence of NA/EU coinfection as well as the possibility of inter-type recombination was carried out. In an epidemiological study, a total of 124 PRRSV strains in which 71 of NA lineage and 53 of EU lineage, were identified between 2001 and 2006 from 54 local pig farms and phylogenetic analysis revealed multiple distinctive clusters existed in both genotypes, possibly due to the multiple introductions of infected swine and purchasing of contaminated frozen semen. In addition, eight full-length genomic sequences (4 NA and 4 EU) of Hong Kong (HK) isolates were analysed and the genomic size of HKNA isolates was characterized to contain 15,411 nucleotides, of which the HKEU isolates have deviated into two sizes: 15,098 and 15,074, where the shortened genome consists of a 24-nucleotide deletion in the hypervariable region located at ORF3/4 overlapping region. Phylogenetic analysis based on ORF3 gene further indicated that the deletion mutants possibly emerged from the non-deleted strains in Hong Kong. It was revealed that 37 tested farms have been coinfected with NA/EU PRRSV from 2001 to 2006, thus characterization of both NA/EU PRRS viruses within a coinfected farm was performed. Among the 35 selected pigs tested, seroconversion was observed but there were no cross-protection detected from heterologous infection. Moreover, the localization of NA and EU PRRS viruses from a persistently coinfected pig was further analyzed by immuno-histochemistry (IHC) and in situ hybridization (ISH). NA/EU coinfection was demonstrated within left cranial and caudal lobe of the lung, but single-cell coinfection was not detected. In this study, the emergence of deletion mutants of EU lineage was identified in Hong Kong pig farms. Together with the full-length sequence analysis, valuable data were provided towards the European PRRS virus genomic characterization which is still very much lacking around the world. Furthermore, cases of NA/EU coinfection have been identified within single pig, suggesting that the infection of one genotype would not exclude the infection of another genotype. In addition, localization of NA and EU PRRS viruses in coinfected tissues, together with negative detection of single-cell coinfection suggested the possibility of the generation of an inter-type recombinant virus in nature is low. However, the interaction of both genotypes on the entry pathway as well as the effects on host immune system remains to be elucidated. DOI: 10.5353/th_b3955817 Subjects: Porcine reproduct

Download or read book Molecular Characterization and Co infection of North American and European Porcine Reproductive and Respiratory Syndrome Virus in Hong Kong written by Yick-yeung Li and published by . This book was released on 2008 with total page 304 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Molecular Characterization and Co infection of North American and European Porcine Reproductive and Respiratory Syndrome Virus in Hong Kong written by and published by . This book was released on 2008 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Molecular and Phylogenetic Analysis of Porcine Reproductive and Respiratory Syndrome Virus Prrsv and Porcine Circovirus Type 2 Pcv2 written by Yick-Yeung Li and published by Open Dissertation Press. This book was released on 2017-01-27 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: This dissertation, "Molecular and Phylogenetic Analysis of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) and Porcine Circovirus Type 2 (PCV2)" by Yick-yeung, Li, 李亦揚, was obtained from The University of Hong Kong (Pokfulam, Hong Kong) and is being sold pursuant to Creative Commons: Attribution 3.0 Hong Kong License. The content of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation. All rights not granted by the above license are retained by the author. Abstract: Abstract of thesis entitled MOLECULAR AND PHYLOGENETIC ANALYSIS OF PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS (PRRSV) AND PORCINE CIRCOVIRUS TYPE 2 (PCV2) Submitted by Yick Yeung LI for the Degree of Master of Philosophy (MPhil) at The University of Hong Kong in November 2003 Porcine reproductive and respiratory syndrome virus (PRRSV) and postweaning multisystemic wasting syndrome (PMWS) have caused a huge economical loss in the global swine industry. The aim of this study is to characterize the prevalence and phylogenetic relationship of local PRRSV and porcine circovirus type 2 (PCV2, causative agent of PMWS), and to develop a DNA vaccine against PRRSV. In this study, PRRSV (26% of tested pigs) and PCV2 (15% of tested pigs) were identified in local swineherds. Phylogenetic analysis on eighteen local PRRSVs from different pig farms demonstrated the existence of both European (45%) and North American (55%) genotypes. Also, the local European typed virus and North American typed virus showed that the amino acid identity of nucleocapsid protein ranged from 89.1% to 95.3% and 94.4% to 98.4% when compared to their respective prototypes. However, the average pairwise genetic distances of the European genotype and North American genotype were found to be 0.047 and 0.065, respectively, suggesting that the PRRSVs with North American genotype was more diverse than those with European genotype. In addition, two sub-branches of European typed PRRSVs and at least four sub-branches of North American typed PRRSVs were identified in local pig farms. Using the phylogenetic tree analysis on the basis of complete nucleotide sequence of local PCV2 viruses and PCV2 isolates around the world, the nucleotide homology of local viruses ranged from 96.1% to 99.9% whereas the nucleotide homology of that with other PCV2 strains ranged from 94.2% to 99.9%. Also, the amino acid identity of the capsid protein of the 10 local PCV2 ranged from 93.2% to 99.6%. Besides, eight PCV2 infected pigs were found to be co-infected with PRRSV. Among them, six were co-infected with North American PRRSV genotypes, one with European genotype and one with both genotypes. To summarize, at least three different PCV2 sub-branches were identified and co-infection with both PRRSV and PCV2 were found in Hong Kong. Apart from the phylogenetic analysis of the two viruses, four mutants with truncated putative signal sequence (Δ 5.4, Δ 5.5, Δ5.6 and Δ5.7) were constructed, based on the hydropathy profile of the PRRSV (strain AV), for DNA vaccine development against PRRSV. Among all mutants, only mutants Δ5.6 and Δ5.7 demonstrated a high level of expression in E. coli. when compared to the full-length (ORF5) construct and were expressed in transfected COS-7 cell lines. Both mutants triggered humoral responses in immunized mice and pigs. In mice, the antibody titer peaked at week 3 and was sustained for at least another three weeks while serum neutralizing antibody was detected in pigs at week 4 and maintained until week 8 at variable levels. Therefore, these two deletion mutants are potential candidates for DNA vaccine development. DOI: 10.5353/th_b2929710 Subjects: Swine - Virus diseases - Genetic aspects DNA vaccines

Download or read book Molecular and Phylogenetic Analysis of Porcine Reproductive and Respiratory Syndrome Virus PRRSV and Porcine Circovirus Type 2 PCV2 written by Yick-yeung Li and published by . This book was released on 2003 with total page 242 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Characterization of Porcine Reproductive and Respiratory Syndrome Virus PRRSV induced Cell Death written by Taeg Su Kim and published by . This book was released on 2001 with total page 140 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Molecular Analysis of the ORFs 3 to 7 of Porcine Reproductive and Respiratory Syndrome Virus Quebec Reference Strain written by H. Mardassi and published by . This book was released on 1995 with total page 14 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Preliminary Characterization of the Transmission of Porcine Reproductive and Respiratory Syndrome Virus written by Robert William Wills and published by . This book was released on 1996 with total page 306 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Molecular Response of Alveolar Macrophages to Porcine Reproductive and Respiratory Syndrome Virus Infection written by Xuexian Zhang and published by . This book was released on 2000 with total page 446 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Structural and Functional Characterization of Porcine Reproductive and Respiratory Syndrome Virus N glycans written by Juan Li and published by . This book was released on 2011 with total page 105 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Characterization of B cell Response to Porcine Reproductive and Respiratory Syndrome Virus Infection in Pigs written by Koti Venkata Ratna Prasad Mulupuri and published by . This book was released on 2006 with total page 154 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Molecular Characterization of Porcine Circovirus written by J. Seetha a/p Jaganathan and published by . This book was released on 2012 with total page 246 pages. Available in PDF, EPUB and Kindle. Book excerpt: