Download or read book Molecular and Functional Characterization of Transgenic Peas Pistum Sativum L Containing Coat Protein CP Genes from Pea Seed borne Mosaic Virus PSBMV and Pea Enation Mosaic Virus PEMV written by Ashapurno Biswas and published by . This book was released on 2005 with total page 180 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Mapping of the Pea Seed borne Mosaic Virus PSbMV Viral Determinant of Resistance in Pisum Sativum Conferred by the Recessive Resistance Gene Sbm 2 written by Charlotte Kristiane Hjulsager and published by . This book was released on 2002 with total page 82 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book The P3 6K1 Protein of Pea Seed borne Mosaic Virus PSbMV and Recessive Resistance Sbm 2 in Pisum Sativum written by Ole Søgaard Lund and published by . This book was released on 2002 with total page 96 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Genetic Relationships Between Pea Seedborne Mosaic Virus Resistance and Horticultural Characteristics Powdery Mildew Enation Mosaic Virus and Red Clover Vein Mosaic Virus Resistance in Pisum Sativum L written by Cathy Hampshire Sewall and published by . This book was released on 1985 with total page 154 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Interactions Between Pea Seed borne Mosaic Virus Pathotype 1 and Pisum Sativum Resistance Gene Sbm 1 written by Karen E. Keller and published by . This book was released on 1995 with total page 156 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Investigations Into the in Vitro Translation and Aphid Transmission of Pea Enation Mosaic Virus written by Clifford J. Gabriel and published by . This book was released on 1983 with total page 218 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Inheritance of Resistance to the Lentil Strain of Pea Seedborne Mosaic Virus in Pisum Sativum L written by Kathryn T. Kasimor and published by . This book was released on 1988 with total page 124 pages. Available in PDF, EPUB and Kindle. Book excerpt: The inheritance mode of pea-resistance to the Lentil Strain of Pea Seedborne Mosaic Virus (PSbMV-L) was studied. In addition, commercial pea cultivars, Plant Introduction (P.I.) lines and Oregon State University (OSU) breeding lines were tested for susceptibility to this virus. These lines were also tested for their reaction to Bean Yellow Mosaic Virus (BYMV) and pathotype 1 of Pea Seedborne Mosaic Virus (PSbMV-Pl) to further elucidate the relationships of Pisum resistance to the three viruses. Susceptible cultivars, 'Sounder', 'Abador', 'Quincy' and 'Avon' were hybridized with resistant cultivars, 'Little Marvel' and OSU B445-66. Data on reaction to PSbMV-L inoculation were obtained for Fl, F2, and F3 progeny and selected backcrosses. Fl tests indicated that resistance was recessive. Most F2 populations segregated as ratios of 3 susceptible: 1 resistant indicating that a single recessive gene controls resistance. Populations of most F3 families fit ratios of 3 susceptible or segregating: 1 resistant. The F3 family data fit ratios of 1 susceptible: 2 segregating: 1 resistant in some of the tests. In remaining tests, failure to fit this expected ratio was assumed to be due to random escapes (susceptible, but not infected), or to a klendusic reaction in progenies from 'Quincy'. Segregation of backcrosses from two crosses in a 1 susceptible: 1 resistant ratio supported the single recessive gene hypothesis. Of 202 commercial cultivars screened screened for PSbMV-L resistance, 144 were resistant and 58 were susceptible. Cultivars resistant to PSbMV-L were also resistant to BYMV, and cultivars susceptible to PSbMV-L were susceptible to BYMV except for 'Quincy' and 'Avon', which were susceptible to PSbMV-L but resistant to BYMV. All of 9 OSU lines and all 32 of selected P.I. lines tested were resistant to PSbMV-L. The P.I. lines had been previously tested and were known to be resistant to PSbMV-Pl. All 9 OSU breeding lines and all but one P.I. line were resistant to BYMV, indicating a possible linkage relationship between resistance to PSbMV-L and BYMV. Three of 178 cultivars, 27 of 32 P.I. lines, and all of 9 OSU breeding lines tested were resistant to both PSbMV-Pl and PSbMV-L. The results suggested that there is a tendency for resistance to PSbMV-Pl and resistance to PSbMV-L to be associated in pea cultivars.

Download or read book Pea Seed borne Mosaic written by Walter R. Stevenson and published by . This book was released on 1972 with total page 246 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Pea Enation Mosaic Virus written by J. Victor French and published by . This book was released on 1973 with total page 186 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Biological Studies with the Pea Enation Mosaic Virus written by Tung Chia Wang and published by . This book was released on 1967 with total page 106 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Pathology and Molecular Comparison of a Range of Pea Seed borne Mosaic Virus Isolates written by Akhtar Ali and published by . This book was released on 1999 with total page 286 pages. Available in PDF, EPUB and Kindle. Book excerpt: This thesis describes the development of serological and nucleic acid based diagnostic methods for pea-seed borne mosaic virus (PSbMV), the isolation of specific effects on infected pea plants, the collection and biological comparison of new PSbMV isolates from Pakistan, the cloning and sequencing of specific parts of the genome of selected isolates, nucleotide and amino acid sequence comparisons between selected isolates, and the development of a ribonuclease protection assay (RPA) for identifying genomic differences among the PSbMV isolates. It is the first comparison of a range of geographically different isolates of PSbMV on the basis of both biological and molecular properties.

Download or read book Genomic Localization of the 5 linked Protein Pg of Pea Enation Mosaic Enamovirus written by Christiane Elisabeth Wobus and published by . This book was released on 1997 with total page 230 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Pea Seed borne Mosaic Virus PSbMV in Field Peas and Lentils written by Michael Johannes Wunsch and published by . This book was released on 2014 with total page 4 pages. Available in PDF, EPUB and Kindle. Book excerpt: Pea seed-borne mosaic virus (PSbMV) is an economically damaging viral pathogen of field peas and lentils that can cause significant losses in seed yield and quality, especially when infections occur before or during bloom. It has been observed on field peas and lentils in North Dakota and on field peas in Montana. PSbMV is distributed worldwide and it presumably was introduced to North Dakota and Montana on seed imported from other regions. PSbMV is seed-transmitted and spread between plants by aphids. When aphid populations are high, planting even low levels of infected seed can result in severe epidemics. Infested seed and the movement of aphids from infested crops are important contributors to the local spread of PSbMV.

Download or read book Anatomical Studies of Pisum Sativum L Infected with Pea Enation Mosaic Virus written by Pamela Wen-chau Wang Tsao and published by . This book was released on 1961 with total page 218 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Characteristics of the Lentil Strain of Pea Seedborne Mosaic Virus written by Jody Jellison Goodell and published by . This book was released on 1983 with total page 250 pages. Available in PDF, EPUB and Kindle. Book excerpt: The lentil strain of pea seedborne mosaic virus, PSbMV-L, was distinguished from the standard isolates of PSbMV by a natural inoculum reservoir in lentil seed and by separate resistance genes in Pisum and Lens. PSbMV-L was intermediate in symptom severity between the standard and New Zealand isolates of PSbMV. Seed transmission rates up to 16.7% were observed for PSbMV-L in seed increase plots of infected lentil accessions, but the virus was not detected in commercial lentil plantings. The virus was nonpersistently transmitted by Acyrthosiphon pisum. Maximum rates of aphid transmission occurred with acquisition access periods of 3 to 5 minutes and ranged from 80 to 100%. Aphid transmission rates decreased with increasing temperature. Apparent trimodal transmission of PSbMV-L was observed. PSbMV-L is characterized by intrinsic particle instability. Partial purification was obtained by extracting infected lentil leaves in a neutral buffer, clarification with 7.5% chloroform, centrifugation through PEG (6 %)- sucrose (35%) cushions and equilibrium centrifugation in CsC1 gradients. The absorbance ratio 260/280 was 1.15. PSbMV-SL25 and PSbMV-L were indistinguishable on the basis of protein subunit molecular weight and could not be differentiated using classical serological techniques. The strains could be separated by quantitative ELISA using polyclonal sera produced in rabbit or chicken. Attempts to achieve greater antibody specificity were made with spleen cells from previously immunized mice that were fused with myoloma cells to create antibody-secreting hybridomas. The resultant colonies were screened using a modified ELISA against PSbMV-SL25 and PSbMV-L for the presence of specific antibody production. Selected colonies were expanded. The molecular weight of PSbMV-L RNA was estimated at 3.5 million. DNA complementary to PSbMV-C4 RNA was prepared using the random primer method of Taylor et al. Hybridization kinetics with PSbMV-L, PSbMV-SL25 and BYMV-Gil RNA were studied using Sl-nuclease assays. The lentil strain showed significantly lower levels of hybridization than the homologous control. Heterologous hybridization was severely decreased when the stringency of the assay conditions was increased.

Download or read book Partial Characterization of the P4 Pathotype of Pea Seedborne Mosaic Virus written by Paul D. Kohnen and published by . This book was released on 1992 with total page 192 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biological, serological, and molecular characteristics of the P4 pathotype of pea seedborne mosaic potyvirus (PSbMV) were investigated. The characterization allowed P4 to be differentiated from other pathotypes of the same virus. P4 was distinguished from P1 on the basis of host symptomatology. It induced varied, but often distinctive symptoms in systemically-infected pea (Pisum sativum) cultivars. P4 inoculation of indicator hosts, notably Chenopodium album, resulted in the formation of local lesions. Pathotype P4 was seed transmitted at low frequencies relative to P1. In ten selected pea cultivars, seed transmission of P4 never exceeded 0.7%, while P1 was transmitted at frequencies as high as 33%. In mixed infections with P1, pathotype P4 was seed transmitted at 0- 2% as determined by P4-specific indirect ELISA. However, limited polymerase chain reaction (PCR) data suggest that P4 may be transmitted with P1 at higher rates than indicated by ELISA data. P4 was also aphid transmitted at relatively low rates. Pea aphids (Acyrthosiphon pisum) allowed 3 or 5 min acquisition access periods (AAP's) transmitted P4 at a maximum rate of 16% (3 aphids/plant). P4 transmission required short (1-7 min) AAP's and exhibited no bimodal character within tested parameters. P4 could be serologically distinguished from the other pathotypes using monoclonal and polyclonal antibodies. Tests with cross-reactive P4 antiserum suggest that P4 is more closely related to P2 than P1. P4-specific antiserum was derived by cross-absorbing diluted antiserum with P1- infected pea sap to eliminate P1- reactive antibodies. P1 and P4 RNA was detected and differentiated using pathotype-specific oligonucleotide primers in conjunction with PCR. Viral RNA sequences were amplified in pure preparations as well as crude pea tissue (leaf, root, pollen, and seed) extracts.

Download or read book Viruses and Virus Diseases of Vegetables in the Mediterranean Basin written by Gad Loebenstein and published by Academic Press. This book was released on 2012-06-25 with total page 596 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume of Advances in Virus Research focuses on mycoviruses. The authors and reviews represent the most current and cutting-edge research in the field. A broad range of research is presented from research experts. Contributions from leading authorities Informs and updates on all the latest developments in the field